BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,856,334	C→A	18.7%	E199* (GAG→TAG) ‡	ydjH ←	putative kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,856,334	0	C	A	18.7%	347.5 / 68.1	143	E199* (GAG→TAG)	ydjH	putative kinase
Reads supporting (aligned to +/- strand): ref base C (56/60); new base A (10/17); total (66/77)
Fisher's exact test for biased strand distribution p-value = 3.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.55e-01

Only 100 of 144 total aligned reads displayed.

CGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAA  >  NC_000913/1856235‑1856432

cGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGGGAGTAATTTTGCCTc                                                                                                    >  2:4329610/1‑100 (MQ=255)

   cTTTACCCGTTTTCATCACCACCGTTTTTACTCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCCCTTTCCCGGTGAGTAATTTTGCCTCGGc                                                                                                 <  1:56973/100‑1 (MQ=255)

      tACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCaaa                                                                                              >  1:2050412/1‑100 (MQ=255)

       aCCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCaaa                                                                                              >  1:1088727/1‑99 (MQ=255)

        cccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAAt                                                                                            <  2:860437/100‑1 (MQ=255)

        cccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAAt                                                                                            >  2:4050212/1‑100 (MQ=255)

         ccattttAATCACCACCGTTTTTACGCCGCCCGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAAtt                                                                                           <  1:1012128/97‑1 (MQ=255)

         ccGTTTTAATCAGCACCGGTTTTGCGCCGCACGCAAGAAGGCAGTCAGCAATTTCATCCGGTGTCTCTTTCCCGGTGAGTCATTTTGCCTCGGCAAAAtt                                                                                           <  1:4208688/100‑1 (MQ=255)

         ccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAAtt                                                                                           <  2:2259262/100‑1 (MQ=255)

         ccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAAtt                                                                                           <  2:1251968/100‑1 (MQ=255)

          cGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTa                                                                                          >  1:1468904/1‑100 (MQ=255)

           gTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTac                                                                                         <  1:4034691/100‑2 (MQ=255)

           gTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTCTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAg                                                                                         <  1:2640700/100‑1 (MQ=255)

             tttAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTactc                                                                                       <  2:1237247/100‑4 (MQ=255)

             tttAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTactc                                                                                       <  1:2761240/100‑4 (MQ=255)

              ttAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTactca                                                                                      <  2:272254/100‑5 (MQ=39)

              ttAATCACCACCGGTTTTACGCCGAACGCAAGAAAGCAGTCAGTAATTACATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAgg                                                                                        <  1:59800/98‑1 (MQ=255)

                aaTCATCACTGTTTTTACGCCGCACGCAAGAAAGGAGTCAGCAATTTCATCCAGTGTCTCTTTCGCGGTGAGTAATTTTGCTGAGGCAAAATTactcacc                                                                                    <  1:3309034/100‑7 (MQ=255)

                aaTCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAAc                                                                                    <  2:4009495/100‑1 (MQ=255)

                aaTCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAAc                                                                                    >  2:288864/1‑100 (MQ=255)

                aaTCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTactcacc                                                                                    <  2:687481/100‑7 (MQ=38)

                aaTCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGCGAGTAATTTTGCCTCGGCAAAATTAGGAAAc                                                                                    >  1:1447545/1‑100 (MQ=255)

                aaTCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAACAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTactcacc                                                                                    <  2:1566637/100‑7 (MQ=38)

                 aTCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACa                                                                                   <  2:2031955/100‑1 (MQ=255)

                   caccacCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGa                                                                                 >  1:397087/1‑100 (MQ=255)

                     ccaccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATa                                                                               >  2:3803911/1‑100 (MQ=255)

                     ccaccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATa                                                                               >  2:4106340/1‑100 (MQ=255)

                     ccaccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATa                                                                               >  2:1604728/1‑100 (MQ=255)

                       accGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAAt                                                                             <  1:3011405/100‑1 (MQ=255)

                       accGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAAt                                                                             >  2:2083090/1‑100 (MQ=255)

                        ccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATc                                                                            <  2:4111558/100‑1 (MQ=255)

                         cGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCg                                                                           >  1:853380/1‑100 (MQ=255)

                         cGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCg                                                                           >  2:2500674/1‑100 (MQ=255)

                         cGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCg                                                                           <  2:3136542/100‑1 (MQ=255)

                         cGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCg                                                                           <  1:4146263/100‑1 (MQ=255)

                         cGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATGAGGAAACAGATAATCg                                                                           >  1:2573908/1‑100 (MQ=255)

                           tttttACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGAc                                                                         <  1:3349317/100‑1 (MQ=255)

                                aCGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAAc                                                                    >  2:268514/1‑100 (MQ=255)

                                    cgcacgcaAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGTAAACAGATAATCGACATAACTCaa                                                                >  1:2139638/1‑100 (MQ=255)

                                     gcacgcaAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAAt                                                               >  1:4096224/1‑100 (MQ=255)

                                     gcacgcaAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAAt                                                               <  2:3311745/100‑1 (MQ=255)

                                     gcacgcaAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAAt                                                               >  1:829336/1‑100 (MQ=255)

                                     gcacgcaAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCCCGGCAAAATTAGGAAACAGATAATCGACATAACTCAAt                                                               <  1:2707527/100‑1 (MQ=255)

                                         gcaAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCtt                                                           >  1:667047/1‑100 (MQ=255)

                                            aGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGc                                                        <  1:2571224/100‑1 (MQ=255)

                                             gAAAGCAGTCAGCAATTTCATCTAGTGTCTCGTTCCCGGTGAGTAATGTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCa                                                       <  1:2968844/100‑1 (MQ=255)

                                             gAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCa                                                       <  2:1973595/100‑1 (MQ=255)

                                              aaaGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCan                                                      <  2:2516616/100‑2 (MQ=255)

                                               aaGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCaaa                                                     <  2:4090398/100‑1 (MQ=255)

                                                aGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAat                                                    <  2:205508/100‑1 (MQ=255)

                                                 gCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAata                                                   <  2:3942539/100‑1 (MQ=255)

                                                 gCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAata                                                   <  2:3038969/100‑1 (MQ=255)

                                                 gCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAata                                                   >  2:2024667/1‑100 (MQ=255)

                                                  caggcaaaaaTTTCAGCCAGGATCTCTTTTCCGTTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATACCTAAATGCTTCGCAAatat                                                  <  1:479265/92‑1 (MQ=255)

                                                     tCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATatcat                                               >  2:2371950/1‑100 (MQ=255)

                                                      cAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATatcatc                                              >  2:1376868/1‑100 (MQ=255)

                                                      cAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCATCGCAAATatcatc                                              >  1:2998151/1‑100 (MQ=255)

                                                        gCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCa                                            >  1:1292492/1‑100 (MQ=255)

                                                         cAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCGTCGCAAATATCATCCAg                                           <  1:132780/100‑1 (MQ=255)

                                                              tCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGttt                                      <  1:1452971/100‑1 (MQ=255)

                                                              tCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGttt                                      <  2:3901006/100‑1 (MQ=255)

                                                              tCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGttt                                      >  2:1686884/1‑100 (MQ=255)

                                                               cATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttc                                     <  2:215414/100‑1 (MQ=255)

                                                                 tCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttcat                                   <  1:4289035/100‑1 (MQ=255)

                                                                  ccAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttcatt                                  >  2:395625/1‑100 (MQ=255)

                                                                  ccAGTGTCTCTTTCCCGGTGAGTAATGTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTTAATGCTTCGCAAATATCATCCAGCGTttcatt                                  <  1:1153123/100‑1 (MQ=255)

                                                                   ctctgtCTCGTTCCCGGTGAGTAATTTTCCCTCGCCAGAATTAAGAAGCAGATATTCGACATATCTCTATGCATCGCAAATATCCTCCATCGTttccttc                                 <  1:3180484/97‑1 (MQ=255)

                                                                   cAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAGATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGAttcatt                                  >  1:1010779/1‑99 (MQ=255)

                                                                     gtgtCTCTTTCCCGGTGAGTAATTTTGCCCCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttcattc                                 <  1:2078836/98‑1 (MQ=255)

                                                                      tgtCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAAc                              <  2:2474882/100‑1 (MQ=255)

                                                                         ctctTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACcgc                           >  1:4356538/1‑100 (MQ=255)

                                                                         ctctTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACcgc                           <  2:2233193/100‑1 (MQ=255)

                                                                          tctTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACcgcg                          >  1:1655253/1‑100 (MQ=255)

                                                                          tctTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACcgcg                          >  2:2122495/1‑100 (MQ=255)

                                                                           ctTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATTACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCgg                         >  2:346775/1‑100 (MQ=255)

                                                                           ctTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCgg                         <  2:1288447/100‑1 (MQ=255)

                                                                           ctTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCgg                         <  2:2050420/100‑1 (MQ=255)

                                                                            tttCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGt                        <  1:3619533/100‑1 (MQ=255)

                                                                              tCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGttt                      <  2:3638935/100‑1 (MQ=255)

                                                                              tCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGttt                      >  1:3659296/1‑100 (MQ=255)

                                                                                ccGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGa                    >  2:4318445/1‑100 (MQ=255)

                                                                                ccGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTCCATTCAACCGCGGTTTGa                    >  2:1115557/1‑100 (MQ=255)

                                                                                  ggTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATc                  <  1:1374488/100‑1 (MQ=255)

                                                                                   gTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCa                 <  2:1394604/100‑1 (MQ=255)

                                                                                    ttcctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTGCATTCAACCGCGGTTTGATCat                >  1:199220/5‑100 (MQ=39)

                                                                                     tcctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCata               >  1:3329582/4‑100 (MQ=255)

                                                                                     tcctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCata               >  2:146116/4‑100 (MQ=255)

                                                                                     tcctAATTTTGCAGAGGCAAAATTGGGAAACAGATAATCGACATAACTGAATGCTTCGCAAATATGATCCAGAGTTTCATTCAAACGCGGTTTAATCata               >  1:3761494/4‑100 (MQ=255)

                                                                                     gAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCata               >  2:3510061/1‑100 (MQ=255)

                                                                                      cctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCatat              >  1:1484070/3‑100 (MQ=255)

                                                                                       gTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATc             >  2:3895625/1‑100 (MQ=255)

                                                                                       ctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATc             >  2:1562848/2‑100 (MQ=255)

                                                                                        tAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCg            <  1:288863/100‑1 (MQ=255)

                                                                                         aaTTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCgg           >  1:159582/1‑100 (MQ=255)

                                                                                         aaTTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCgg           >  2:2031537/1‑100 (MQ=255)

                                                                                          aTTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGc          <  2:1037367/100‑1 (MQ=255)

                                                                                             ttGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGcaca       <  2:2584551/100‑1 (MQ=255)

                                                                                                ncTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGAAAATATCATCAAGCGTTACATTCAACCGCGGTTTGATCATATCGGCACAgct    >  1:1566150/2‑98 (MQ=255)

                                                                                                 cTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATa   >  2:3758436/1‑100 (MQ=255)

                                                                                                  tCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATaa  >  1:209094/1‑100 (MQ=255)

CGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAA  >  NC_000913/1856235‑1856432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`