Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,861,419 A→G 15.1% intergenic (‑87/+283) ydjL ← / ← yeaC putative Zn‑dependent NAD(P)‑binding oxidoreductase/DUF1315 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,861,4190AG15.1% 322.7 / 41.5 132intergenic (‑87/+283)ydjL/yeaCputative Zn‑dependent NAD(P)‑binding oxidoreductase/DUF1315 family protein
Reads supporting (aligned to +/- strand):  ref base A (55/56);  new base G (16/4);  total (72/60)
Fisher's exact test for biased strand distribution p-value = 1.44e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 138 total aligned reads displayed.

CAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAA  >  NC_000913/1861321‑1861517
                                                                                                  |                                                                                                  
cAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAacac                                                                                                   <  1:4163354/100‑1 (MQ=255)
cAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAacac                                                                                                   >  2:3226778/1‑100 (MQ=255)
 aGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAAcaca                                                                                                  >  2:897357/1‑100 (MQ=255)
 aGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAAcaca                                                                                                  >  1:2055257/1‑100 (MQ=255)
   tGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGa                                                                                                <  2:781129/100‑1 (MQ=255)
    gCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGAt                                                                                               <  2:951258/100‑1 (MQ=255)
     cTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATa                                                                                              <  2:1081573/100‑1 (MQ=255)
     cTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATa                                                                                              >  2:2892599/1‑100 (MQ=255)
     cTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATa                                                                                              >  2:2661014/1‑100 (MQ=255)
       ttCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAAt                                                                                            <  1:4214976/100‑1 (MQ=255)
       ctCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAAt                                                                                            <  1:2059369/99‑1 (MQ=255)
         cATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAAtag                                                                                          >  2:2059377/1‑98 (MQ=255)
         cATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGa                                                                                          <  2:500181/100‑1 (MQ=255)
          aTTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGAt                                                                                         >  2:3916755/1‑100 (MQ=255)
          aTTGCGTCAGCCTCTGGGTATAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGAt                                                                                         >  1:3870984/1‑100 (MQ=255)
           ttGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGAt                                                                                         >  1:3274785/1‑99 (MQ=255)
              cGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTGAACATTAAATCTGTAAAACACAGATAATGATc                                                                                        >  1:1101098/1‑97 (MQ=255)
                  aTCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACGCAGATCATTATCTGTGttt                                                                                 <  2:3044593/100‑1 (MQ=38)
                   tCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGtttt                                                                                >  1:4015185/1‑100 (MQ=255)
                    ccTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTTTTTTTTTTTTTAAATCTGTAAAACACAGATAATGATCTGCGTTTTa                                                                               >  1:776021/1‑100 (MQ=255)
                    ccTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTa                                                                               >  2:537839/1‑100 (MQ=255)
                     ctctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTAc                                                                              <  2:4292567/100‑1 (MQ=255)
                      tctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACa                                                                             >  1:1589817/1‑100 (MQ=255)
                        tgtgTTTAGTCTTGCTTAGATGGCAATAATACTTACTCAAGTGAGTTTTAAGGTCAACATTAAATATGTAAAACACAAATAATGATCTCCGTATTACaa                                                                            >  1:2776677/1‑99 (MQ=255)
                          tgtTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACGCAGATCATTATCTGTGTTTTACagatt                                                                         <  1:973755/100‑5 (MQ=37)
                           gtTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGAGttgtaaaacgca                                                                        <  1:1386455/100‑11 (MQ=255)
                            tttAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAg                                                                       >  1:1665882/1‑100 (MQ=255)
                            tttAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAg                                                                       <  2:3550321/100‑1 (MQ=255)
                            ttgaGTTTTGCTTAGATGACCAGCATACTTTCTCACAGGAGTTGTGAGGTCAACATTATAGATGTAAAACGCAGATCATTATCTTTGTTTTACagat                                                                          <  1:2810219/94‑4 (MQ=9)
                               aGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATc                                                                    <  1:746459/100‑1 (MQ=255)
                                  ttttCTTAGATGGCCAACACACTTACCCACATGATTTTTCAAGTTAACACTACTTCTGTAAACCAAAGATAACAAACTGCGATTTACACCTCAg                                                                       >  1:2311077/1‑94 (MQ=255)
                                  tttGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATcaca                                                                 <  2:28116/100‑1 (MQ=255)
                                   ttGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGAGTTGTAAAACGCAGATcatta                                                                <  2:283672/100‑4 (MQ=38)
                                    tGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAAt                                                               >  2:622486/1‑100 (MQ=255)
                                    tGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAAt                                                               >  1:3332483/1‑100 (MQ=255)
                                     gCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTCAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAAtt                                                              >  1:2813728/1‑100 (MQ=255)
                                      cTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTa                                                             >  2:2903462/1‑100 (MQ=255)
                                        tAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACg                                                           <  1:1523884/100‑1 (MQ=255)
                                         aGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGc                                                          <  2:1665887/100‑1 (MQ=255)
                                          taTGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCa                                                         <  1:3327135/99‑1 (MQ=255)
                                           aTGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTCCGCAg                                                        <  1:593810/100‑1 (MQ=255)
                                             ggCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGaa                                                      >  2:3595303/1‑100 (MQ=255)
                                             ggCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGaa                                                      >  1:4014445/1‑100 (MQ=255)
                                               ccATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGaaca                                                    >  2:2266951/1‑98 (MQ=255)
                                               ccATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGaaaa                                                    >  1:4140060/1‑100 (MQ=255)
                                                catcatACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAAt                                                   >  1:3077374/1‑100 (MQ=255)
                                                catcatACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAAt                                                   >  2:2314071/1‑100 (MQ=255)
                                                 atcatACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATg                                                  <  2:3740466/100‑1 (MQ=255)
                                                 atcatACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATg                                                  >  1:3537710/1‑100 (MQ=255)
                                                  tcatACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATgg                                                 <  2:2523/100‑1 (MQ=255)
                                                   catACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGc                                                >  1:559621/1‑100 (MQ=255)
                                                       cTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTggg                                            >  2:4105544/1‑100 (MQ=255)
                                                       cTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGAGCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTggn                                            <  2:1918209/100‑2 (MQ=255)
                                                         tACCCACATGATTTTTTAGTTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTc                                          >  1:3459246/1‑100 (MQ=255)
                                                         tACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTc                                          >  1:2523125/1‑100 (MQ=255)
                                                         tACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTc                                          <  2:706326/100‑1 (MQ=255)
                                                           cccACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTg                                        <  1:3457477/100‑1 (MQ=255)
                                                           cccACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTg                                        <  1:4267076/100‑1 (MQ=255)
                                                            tcacaTGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGt                                       <  1:1913850/99‑1 (MQ=255)
                                                             cacaTGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGtt                                      >  1:1027108/1‑100 (MQ=255)
                                                             cacaTGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGt                                       >  1:2204872/1‑99 (MQ=255)
                                                             cacaTGATTTTTGAGGTCAACATAACATCTGTAAAACCCAGATGATGATCTGCGTTTTACCACTCAGCACACAATTAGGCAGAAAATTGCTGGGTCt                                         >  1:3754445/1‑97 (MQ=255)
                                                               caTGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTa                                     <  1:1731804/99‑1 (MQ=255)
                                                                aTGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACa                                   <  1:107018/100‑1 (MQ=255)
                                                                 tGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAg                                  <  2:2360786/100‑1 (MQ=255)
                                                                 tGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAg                                  >  2:828690/1‑100 (MQ=255)
                                                                 tGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAg                                  <  1:4102226/100‑1 (MQ=255)
                                                                  gATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAgg                                 <  2:1721253/100‑1 (MQ=255)
                                                                        tGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATg                           <  2:2813744/100‑1 (MQ=255)
                                                                        tGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATg                           <  2:4162862/100‑1 (MQ=255)
                                                                         gAGGTCAACATTAAATCTGTGAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATgg                          >  1:2693043/1‑100 (MQ=255)
                                                                         gAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAGTGGCTGGGTCTGTTACAGGTTGATgg                          >  1:2217018/1‑100 (MQ=255)
                                                                           ggTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGttagatcgg                        >  1:1654210/1‑93 (MQ=255)
                                                                           ggTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGaa                        >  2:2667409/1‑100 (MQ=255)
                                                                                aCATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCgg                   <  1:1868318/100‑1 (MQ=255)
                                                                                aCATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCgg                   <  2:3537729/100‑1 (MQ=255)
                                                                                 gatctgagttGTAAAACGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCggg                  >  2:508615/10‑100 (MQ=21)
                                                                                  aTTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCgggg                 >  2:4364035/1‑100 (MQ=255)
                                                                                  aTTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCGGTTACAGGTTGATGGAAGGCgggg                 <  1:2428394/100‑1 (MQ=255)
                                                                                    tAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGgcg               <  1:1508196/100‑1 (MQ=255)
                                                                                    tAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGgcg               <  2:1273864/100‑1 (MQ=255)
                                                                                     aaaTCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGgcgc              <  2:1087408/100‑1 (MQ=255)
                                                                                      gagttGTAAAACGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCa             >  2:106594/5‑100 (MQ=25)
                                                                                       aTCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCaa            <  2:2536305/100‑1 (MQ=255)
                                                                                         ttGTAAAACGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCaaaa          >  1:2392221/2‑100 (MQ=37)
                                                                                          tGTAAAACGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCaaaaa         >  1:3812335/1‑100 (MQ=38)
                                                                                          tGTAAAACGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCaaaaa         >  1:9418/1‑100 (MQ=38)
                                                                                          tGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCaaaaa         >  2:1447394/1‑100 (MQ=255)
                                                                                           gTAAAACGCAGATCATTATCTGTGTTTTACACCTCAGATCACAATTACGCAGAAAATGTCTGTGTCTGTAACAGGGTGAGGGAAGGCGGGGCGCAAAAag        >  1:1388433/1‑100 (MQ=25)
                                                                                           gTAAAACGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAag        >  1:4333225/1‑100 (MQ=38)
                                                                                           gTAAAACGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAag        >  2:813158/1‑100 (MQ=38)
                                                                                           gTAAAACGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAag        >  1:2375351/1‑100 (MQ=38)
                                                                                            tAAAACGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAaga       >  2:429851/1‑100 (MQ=38)
                                                                                            tAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAaga       >  2:352593/1‑100 (MQ=255)
                                                                                            tAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAaga       <  2:1977620/100‑1 (MQ=255)
                                                                                            tAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGCTTGATGGAAGGCGGGGCTCAAAAag        <  1:1353507/99‑1 (MQ=255)
                                                                                             aaaaCACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAagag      <  2:2275883/100‑1 (MQ=255)
                                                                                              aaaCGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGc     >  2:170676/1‑100 (MQ=38)
                                                                                                 cacaGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCaaa  >  1:3194512/1‑100 (MQ=255)
                                                                                                 cacaGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCaaa  >  1:967969/1‑100 (MQ=255)
                                                                                                  |                                                                                                  
CAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAA  >  NC_000913/1861321‑1861517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: