Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,950,306 C→G 15.4% G73R (GGC→CGC)  aspS ← aspartyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,950,3060CG15.4% 426.0 / 67.3 163G73R (GGC→CGC) aspSaspartyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (64/73);  new base G (18/7);  total (83/80)
Fisher's exact test for biased strand distribution p-value = 2.85e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.75e-02

Only 100 of 165 total aligned reads displayed.

GCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTCGCGGTC  >  NC_000913/1950209‑1950405
                                                                                                   |                                                                                                   
gcgcGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCg                                                                                                   >  1:3977982/1‑100 (MQ=255)
gcgcGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCg                                                                                                   <  2:3438354/100‑1 (MQ=255)
 cgtggTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCg                                                                                                   <  1:2532180/96‑1 (MQ=255)
  gcgGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTg                                                                                                 <  2:253426/100‑1 (MQ=255)
     gTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGC‑ACCGTAC‑GTGCGCGTGAcg                                                                                              <  2:3911784/100‑2 (MQ=38)
     gTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGtta                                                                                                >  1:1719680/1‑96 (MQ=255)
     gTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGAcc                                                                                              <  2:1072081/100‑1 (MQ=255)
       tgattaTCGTCAGCGAGGACGCCGGTAATTCGATTACGCGGGTCGCCATATCGCGGTTAATTTTTTTTTCGTCACGGGCTC‑CGTAC‑GTGCGCGTGAcgaa                                                                                            <  1:99127/100‑4 (MQ=16)
       tgatgaTAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGC‑ACCGTAC‑GTGCGCGTGAcgaa                                                                                            <  2:588472/100‑4 (MQ=37)
       tgatgaTAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGC‑ACCGTAC‑GTGCGCGTGAcgaa                                                                                            <  2:4326837/100‑4 (MQ=37)
       tgatgaTAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTg                                                                                            <  1:1543737/100‑1 (MQ=255)
         atgatAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGaa                                                                                          >  2:4052007/1‑100 (MQ=255)
         atgatAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGaa                                                                                          >  2:3978640/1‑100 (MQ=255)
          tgatAGTCAGCGAGGACGCCAGAACTTCGATTTAGTCGGTGGCCATATCGCGGTTAATATTTTTTTCGTTACGCGC‑A‑CGTAAGGTGGCCGTGACCTGaa                                                                                          >  1:2651104/1‑99 (MQ=255)
             tAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGc                                                                                       <  1:2246495/99‑1 (MQ=255)
             tAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCa                                                                                      <  2:2132309/100‑1 (MQ=255)
              aggcAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAg                                                                                     <  1:1051647/97‑1 (MQ=255)
               gTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGa                                                                                    <  1:924193/100‑1 (MQ=255)
               gTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGa                                                                                    >  1:1804965/1‑100 (MQ=255)
               gTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGa                                                                                    >  2:3481154/1‑100 (MQ=255)
                 cAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAAc                                                                                  >  2:1598245/1‑100 (MQ=255)
                  aGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAAc                                                                                  >  1:70664/1‑99 (MQ=255)
                  aGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACt                                                                                 <  1:2672211/100‑1 (MQ=255)
                    cGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCa                                                                               <  1:1058035/100‑1 (MQ=255)
                    cGAGGACGCCAGCACTTCGATATCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATACAGAACTCa                                                                               >  1:4264138/1‑100 (MQ=255)
                    cGAGGACGCCAGCACTTCGATATCGCCGGTAGCCATATCGCGGTTAATATTTTTTTCTTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCa                                                                               >  1:498005/1‑100 (MQ=255)
                       ggACGCCAGCCCTTCGATTTCTCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACACGC‑A‑CGTACGGTGCACGTGACCTGATTTCAGAACTCATTa                                                                            >  1:3816659/1‑100 (MQ=255)
                        gACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTAc                                                                           <  2:3344185/100‑1 (MQ=255)
                         aCGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACg                                                                          <  1:226788/100‑1 (MQ=255)
                          cGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGc                                                                         >  2:260646/1‑100 (MQ=255)
                          cGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGc                                                                         >  2:260967/1‑100 (MQ=255)
                           gCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCa                                                                        >  1:4067131/1‑100 (MQ=255)
                            ccAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAg                                                                       <  2:958163/100‑1 (MQ=255)
                              aGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTa                                                                            >  1:1034375/1‑93 (MQ=255)
                               gCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTc                                                                    >  2:3637710/1‑100 (MQ=255)
                                  cTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCaga                                                                 >  1:2412858/1‑100 (MQ=255)
                                    tCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAgg                                                               <  2:524176/100‑1 (MQ=255)
                                     cGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGc                                                              >  1:3759312/1‑100 (MQ=255)
                                      gATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGcc                                                             >  1:2023085/1‑100 (MQ=255)
                                      gATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGcc                                                             <  2:948377/100‑1 (MQ=255)
                                      gATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGcc                                                             >  1:128693/1‑100 (MQ=255)
                                       aTTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCa                                                            <  1:562576/100‑1 (MQ=255)
                                        tttCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAg                                                           >  2:2203726/1‑100 (MQ=255)
                                        tttCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAg                                                           <  1:1161912/100‑1 (MQ=255)
                                         ttCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGCCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGc                                                          <  1:1923430/100‑1 (MQ=255)
                                          tCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGc                                                          >  1:529914/1‑99 (MQ=255)
                                          tCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCt                                                         >  1:1671441/1‑100 (MQ=255)
                                          tCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCt                                                         <  1:2104144/100‑1 (MQ=255)
                                            gCCGGTCGCCATATCGCGGTTAATATTTTTTTCTTCACGTGC‑A‑CGTACGGTGCCCGTGACCTCAATGCAGACCTCATTACGAAGTTCAGAGGCCAGCttt                                                       >  1:677124/1‑100 (MQ=255)
                                            gCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCttt                                                       <  2:4067160/100‑1 (MQ=255)
                                            gCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCttt                                                       >  2:1273122/1‑100 (MQ=255)
                                               ggTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAAc                                                    <  2:3080717/100‑1 (MQ=255)
                                                gTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAAcg                                                   >  1:3465046/1‑100 (MQ=255)
                                                  cGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAAcgcg                                                 <  1:2203718/100‑1 (MQ=255)
                                                  cGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAAcgcg                                                 >  2:418233/1‑100 (MQ=255)
                                                    ccATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTGAAcgcg                                                 >  2:491899/1‑98 (MQ=255)
                                                      atatCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCg                                             <  2:902835/100‑1 (MQ=255)
                                                        atCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCa                                           >  1:1558931/1‑100 (MQ=255)
                                                          cgcgGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGCCCTGAATGCAGAACTCATTAAGCAGTTCAAAGGCCCGCTTTAACGCGTCCGCACg                                         >  2:2355527/1‑100 (MQ=255)
                                                           gcgGTTAATATTTTTTTCGTCACGCGC‑C‑CGTACGGTGCCCGTAACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGCCCGCACGa                                        >  2:2656956/1‑100 (MQ=255)
                                                                 aaTTTTTTTTTCCTCACGCGC‑A‑CGTACGTGGCCCGTGCCCTGAGTGCAGACCTCATCACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCgca                                  <  1:2827036/100‑3 (MQ=255)
                                                                  atatTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGAt                                 >  2:3021393/1‑100 (MQ=255)
                                                                    atTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCg                               <  2:1278957/100‑1 (MQ=255)
                                                                    atTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCAGTGACCTGGATGCAGAACACATCACGCAGTTCTGAGGCCAGCTATAACGCGTCGGCCCGAGCAGGacc                                >  1:3614158/1‑97 (MQ=255)
                                                                     tttttttCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCCGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGa                              >  2:1692606/1‑100 (MQ=255)
                                                                       tttttCGTCACGCGC‑A‑CGTACGGTGCCCGTGTCCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGaaa                            <  1:102825/100‑1 (MQ=255)
                                                                       tttttCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGaaa                            <  1:300047/100‑1 (MQ=255)
                                                                       tttttCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGaaa                            <  2:2008275/100‑1 (MQ=255)
                                                                       tgtttCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGGCCGCACGATCCGGATCGaaa                            <  1:113921/98‑1 (MQ=255)
                                                                        ttttCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGaaaa                           <  2:2850803/100‑1 (MQ=255)
                                                                           tCGTCACGCGC‑A‑CGTACGGTGCCCGGGACCTGAACGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGAACCGGATCGAAAAATa                        <  1:3183759/100‑1 (MQ=255)
                                                                            ggTCACGGGC‑ACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAc                       >  2:1642417/2‑100 (MQ=38)
                                                                            ggTCACGGGC‑ACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAc                       >  2:154344/2‑100 (MQ=38)
                                                                            cGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATa                        <  2:2818952/99‑1 (MQ=255)
                                                                            cGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAc                       >  1:3335356/1‑100 (MQ=255)
                                                                            cGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGAGTCCGGACGATCAGGATCGAAAAATa                        >  1:1142083/1‑99 (MQ=255)
                                                                             gTCACGGGC‑ACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc                      >  1:237733/1‑100 (MQ=38)
                                                                             gTCACGGGC‑ACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc                      >  1:3307411/1‑100 (MQ=38)
                                                                             gTCACGGGC‑ACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc                      >  1:2483560/1‑100 (MQ=38)
                                                                             gTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc                      >  2:1325404/1‑100 (MQ=255)
                                                                             gTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc                      >  2:3581431/1‑100 (MQ=255)
                                                                             atCACGGGC‑ACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAAAGCGTCCGCACGATCCGGATCGAAAAATa                        >  1:2295573/2‑98 (MQ=37)
                                                                               cACGGGC‑ACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTg                    >  1:7800/1‑100 (MQ=255)
                                                                               cACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTg                    <  1:4092077/100‑1 (MQ=255)
                                                                                aCGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGc                   <  1:3867656/100‑1 (MQ=255)
                                                                                 cgcgC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGAGCGAAAAATACCTGCa                  >  2:4333092/1‑100 (MQ=255)
                                                                                 acgggcaccGTAC‑GTGCGCGTGACCTGAATGCAGCACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGc                   >  2:2186332/9‑100 (MQ=255)
                                                                                   cgc‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACg                <  2:2289399/100‑1 (MQ=255)
                                                                                         cGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATAc            >  1:2393224/1‑100 (MQ=255)
                                                                                         cGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATAc            <  2:803741/100‑1 (MQ=255)
                                                                                           tACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCt          >  1:3024614/1‑100 (MQ=255)
                                                                                           tACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCt          >  2:1960304/1‑100 (MQ=255)
                                                                                              ggTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTcg       >  2:4222828/1‑100 (MQ=255)
                                                                                               gTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCt          >  1:404424/1‑96 (MQ=255)
                                                                                               gTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTcgc      <  1:418231/100‑1 (MQ=255)
                                                                                               gTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTcg       >  2:1522053/1‑99 (MQ=255)
                                                                                                 gCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTCGCgg    <  2:3969553/100‑1 (MQ=255)
                                                                                                 gCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTCGCgg    <  2:2890724/100‑1 (MQ=255)
                                                                                                 gCCCGTGACCTGAATGCAGAACTCACTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCCCGATCCGGATCGAAAAATACCTGCACGATACCTTCGCgg    <  1:3883063/100‑1 (MQ=255)
                                                                                                   ccGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTCGCGGTc  <  2:2345433/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
GCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGC‑A‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTCGCGGTC  >  NC_000913/1950209‑1950405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑