Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,028,159 T→A 17.8% intergenic (‑142/+29) yedQ ← / ← yodC putative membrane‑anchored diguanylate cyclase/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,028,1590TA17.8% 269.7 / 48.1 112intergenic (‑142/+29)yedQ/yodCputative membrane‑anchored diguanylate cyclase/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (46/46);  new base A (7/13);  total (53/59)
Fisher's exact test for biased strand distribution p-value = 3.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.47e-01

Only 100 of 122 total aligned reads displayed.

CTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGA  >  NC_000913/2028060‑2028258
                                                                                                   |                                                                                                   
cTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGAtgt                                                                                                     >  1:1347302/1‑100 (MQ=255)
cTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGAtgt                                                                                                     <  1:2171977/100‑1 (MQ=255)
  ggCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGa                                                                                                   <  2:3963847/100‑1 (MQ=255)
    ngATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATc                                                                                                 >  2:1020120/2‑100 (MQ=255)
    cGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATc                                                                                                 >  2:692961/1‑100 (MQ=255)
    cGATAGCGTACCGCAAAACAGCGATAATTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATcc                                                                                                >  2:3884619/1‑100 (MQ=255)
       tAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCgg                                                                                              <  1:312711/100‑1 (MQ=255)
         gCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGcc                                                                                            <  1:3406550/100‑1 (MQ=255)
         gCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGcc                                                                                            >  2:638992/1‑100 (MQ=255)
         gCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGcc                                                                                            >  2:638767/1‑100 (MQ=255)
         gCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGcc                                                                                            <  2:2099253/100‑1 (MQ=255)
          cGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACATCAGGCCGGATCACATCCGGCCt                                                                                           <  1:4398074/100‑1 (MQ=38)
          cGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACATCAGGCCGGATCACATCCGGCCt                                                                                           <  2:1644696/100‑1 (MQ=38)
          cGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCt                                                                                           <  2:48496/100‑1 (MQ=255)
          cGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCt                                                                                           <  2:691410/100‑1 (MQ=255)
          cGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCt                                                                                           <  1:2190504/100‑1 (MQ=255)
          cGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATCACATCCGGCCt                                                                                           <  1:2676646/100‑1 (MQ=255)
          cGTACCGCAAAACAGCGATAATCTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATCACATCCGGCCt                                                                                           <  2:3422835/100‑1 (MQ=255)
           gTACCGCTAAAAAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTg                                                                                          >  1:2301938/1‑100 (MQ=255)
           gTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTg                                                                                          <  1:2883879/100‑1 (MQ=255)
           gTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATCACATCCGGCCTg                                                                                          <  2:1856450/100‑1 (MQ=255)
           gTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATCACATCCGGCCTg                                                                                          <  2:2033701/100‑1 (MQ=255)
            tACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTg                                                                                          >  2:3602267/1‑99 (MQ=255)
            tACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGa                                                                                         <  2:2820577/100‑1 (MQ=255)
            tACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATCACATCCGGCCTgg                                                                                         <  2:3067435/100‑2 (MQ=255)
              ccGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATg                                                                                       >  1:3535739/1‑100 (MQ=255)
               cGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACATCAGGCCGGATCACATCCGGCCTGgtgt                                                                                      <  1:2926269/100‑1 (MQ=38)
                gCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGtt                                                                                     >  2:1469136/1‑100 (MQ=255)
                gCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGtt                                                                                     >  2:1408098/1‑100 (MQ=255)
                 cAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACATCAGGCCGGATCACATCCGGCCTGgtgtgc                                                                                    <  2:3409330/100‑3 (MQ=37)
                  aaagCAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACATCAGGCCGGATCACATCCGGCCTGgtgtgc                                                                                    <  1:2988943/99‑3 (MQ=25)
                   aaaCAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACATCAGGCCGGATCACATCCGGCCTGgtgtgc                                                                                    <  1:2989159/98‑3 (MQ=37)
                   aaaCAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAAt                                                                                  <  2:3686394/100‑1 (MQ=255)
                   aaaCAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAAt                                                                                  <  2:1141156/100‑1 (MQ=255)
                    aaCAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATg                                                                                 >  2:1933332/1‑100 (MQ=255)
                    aaCAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATg                                                                                 <  1:2202674/100‑1 (MQ=255)
                       aGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATa                                                                              >  1:2247231/1‑100 (MQ=255)
                       aGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATa                                                                              >  2:2466846/1‑100 (MQ=255)
                        gCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATAc                                                                             >  1:3876494/1‑100 (MQ=255)
                         cGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATAcc                                                                            <  1:2936116/100‑1 (MQ=255)
                         cGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATAcc                                                                            >  1:2633473/1‑100 (MQ=255)
                            tAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCtt                                                                         <  2:343057/100‑1 (MQ=255)
                               ttttATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTca                                                                      <  1:3435953/100‑1 (MQ=255)
                               ttttATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTca                                                                      >  1:1423564/1‑100 (MQ=255)
                               ttttATAATGGTGGCATGATAAGACGCGACTATCATTCATTACAGGCATTAGCACACAAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTca                                                                      >  1:3720755/1‑100 (MQ=255)
                                tttATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTcac                                                                     <  1:661926/100‑1 (MQ=255)
                                  tataATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCacac                                                                   <  2:221489/100‑1 (MQ=255)
                                    taATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACActt                                                                 >  1:261660/1‑100 (MQ=255)
                                        ggtggCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGGCTGATGTTAATGATACCCTTTCACACttcttc                                                             <  2:3816103/100‑1 (MQ=255)
                                            gCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGc                                                          >  1:3391536/1‑99 (MQ=255)
                                             cATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAg                                                        <  2:1949142/100‑1 (MQ=255)
                                                      aCGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActcc                                               <  1:769265/100‑1 (MQ=255)
                                                       cgcgACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActccc                                              <  1:2743405/100‑1 (MQ=255)
                                                             tATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActcccctccc                                         <  1:1700645/99‑1 (MQ=255)
                                                               tcattcatGCAGCCATTAGCACACCAGGCCGGATGTGCTCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTccccc                                       <  1:1765022/98‑1 (MQ=255)
                                                                cattcattGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCgg                                     >  2:2863279/1‑100 (MQ=255)
                                                                cattcattGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCgg                                     >  2:1425555/1‑100 (MQ=255)
                                                                cattcattGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCgg                                     >  1:1632662/1‑100 (MQ=255)
                                                                 attcattGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGa                                    >  1:1705135/1‑100 (MQ=255)
                                                                 attcattGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGa                                    >  1:2799394/1‑100 (MQ=255)
                                                                 attcattGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGa                                    >  2:1629040/1‑100 (MQ=255)
                                                                  ttcattGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGaa                                   <  2:865235/100‑1 (MQ=255)
                                                                     attGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACaa                                <  1:844368/100‑1 (MQ=255)
                                                                        gCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGc                              >  2:22150/1‑99 (MQ=255)
                                                                         cAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTc                            >  2:2234322/1‑100 (MQ=255)
                                                                          aGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCg                           >  2:4095552/1‑100 (MQ=255)
                                                                          aGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCg                           <  1:3522252/100‑1 (MQ=255)
                                                                          aGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCg                           <  2:808838/100‑1 (MQ=255)
                                                                           ggCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACTCTTCTTCCGCAGAACGTCTCCCCTCCCCCGGAACAAGCTCgg                          >  1:3213935/1‑100 (MQ=255)
                                                                           ggCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCgg                          <  2:1331962/100‑1 (MQ=255)
                                                                           ggCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCgg                          >  1:1159589/1‑100 (MQ=255)
                                                                              aTTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGttt                       <  2:499914/100‑1 (MQ=255)
                                                                              aTTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGttt                       <  2:496919/100‑1 (MQ=255)
                                                                               ttAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTc                      <  1:3886375/100‑1 (MQ=255)
                                                                                tAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCa                     >  2:2131630/1‑100 (MQ=255)
                                                                                attaacatcAGGCCGGATCACATCCGGCCTGGTGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCa                     >  1:1388873/9‑100 (MQ=25)
                                                                                 ttaacatCAGGCCGGATCACATCCGGCCTGGTGTTAATGCTACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCAt                    >  2:1789811/4‑100 (MQ=25)
                                                                                 aGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCAt                    <  2:3609688/100‑1 (MQ=255)
                                                                                 aGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCAt                    <  1:2055722/100‑1 (MQ=255)
                                                                                 aGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCAt                    >  1:171274/1‑100 (MQ=255)
                                                                                    acatCAGGCCGGATCACATCCGGCCTGGTGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTCTCATGaa                 >  1:4027514/1‑100 (MQ=37)
                                                                                    acacCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGaa                 <  2:3201923/100‑1 (MQ=255)
                                                                                     catcAGGCCGGATCACATCCGGCCTGGTGTTAATGATACCCTTTCACACTTCTTCCGGAGGACGACTGCCCTCCCCCGGAACAAGCTCGGTTTCATGaaa                >  1:3087463/4‑100 (MQ=25)
                                                                                     catcAGGCCGGATCACATCCGGCCTGATATTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCa                     >  1:1365402/4‑95 (MQ=38)
                                                                                     cacCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGaaa                >  1:2748874/1‑100 (MQ=255)
                                                                                      cccAGGCCGCCAGTGCTCCGGCCTGAGTTGAATGATGCCCCTTCACACTTCGTCCGCAGAACGACTCCCCCCCCCCGGAACAAGCTCGGTTTCAt                    <  1:3631013/94‑1 (MQ=255)
                                                                                      acCTGGCCGGATGTGATCCGGCCCGATGTTAATGCTACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATg                   <  1:246585/96‑1 (MQ=255)
                                                                                      acCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACCTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGaaaa               <  1:3946861/100‑1 (MQ=255)
                                                                                       tcAGGCCGGATCACATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAg              >  2:2851220/2‑100 (MQ=255)
                                                                                       ccAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAg              >  1:4104327/1‑100 (MQ=255)
                                                                                         aGGCCGGATGTGATCCGGCATGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAAGTACTACCCTCCCCCGGAACAAGATCGGTTTCATGAAAAGc             >  1:2927989/1‑99 (MQ=255)
                                                                                          ggCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCtt           >  1:3380942/1‑100 (MQ=255)
                                                                                            ccGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTcc         >  2:2678958/1‑100 (MQ=255)
                                                                                                aTGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCt     <  2:1362776/100‑1 (MQ=255)
                                                                                                 tgtgATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCtt    <  1:4198192/100‑1 (MQ=255)
                                                                                                  gtgATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTg   <  2:458486/100‑1 (MQ=255)
                                                                                                  gtgATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTg   >  2:3502914/1‑100 (MQ=255)
                                                                                                  gtgATCCGGCCTGATGTTAATGACACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTg   >  1:2917003/1‑100 (MQ=255)
                                                                                                   tgATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGa  >  1:3299696/1‑100 (MQ=255)
                                                                                                   tgATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGa  >  1:3953593/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
CTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGA  >  NC_000913/2028060‑2028258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: