Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,081,688 A→T 17.0% I287N (ATT→AAT)  dacD ← D‑alanyl‑D‑alanine carboxypeptidase; penicillin‑binding protein 6b

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,081,6880AT17.0% 350.5 / 61.7 146I287N (ATT→AAT) dacDD‑alanyl‑D‑alanine carboxypeptidase; penicillin‑binding protein 6b
Reads supporting (aligned to +/- strand):  ref base A (58/63);  new base T (15/10);  total (73/73)
Fisher's exact test for biased strand distribution p-value = 3.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01

Only 100 of 151 total aligned reads displayed.

GGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGA  >  NC_000913/2081589‑2081787
                                                                                                   |                                                                                                   
 gTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAAt                                                                                                    >  1:4238343/1‑100 (MQ=255)
    gCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTg                                                                                                 <  2:3263290/100‑1 (MQ=255)
    gCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTg                                                                                                 <  1:3478535/100‑1 (MQ=255)
    gCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTg                                                                                                 >  2:4078462/1‑100 (MQ=255)
    gCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTg                                                                                                 >  2:693029/1‑100 (MQ=255)
     cACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGc                                                                                                <  2:1789109/100‑1 (MQ=255)
      aCCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTTTCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCa                                                                                               >  1:3115649/1‑100 (MQ=255)
       ccATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCAc                                                                                              <  1:2643993/100‑1 (MQ=255)
       ccATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCAc                                                                                              <  1:1218929/100‑1 (MQ=255)
        cATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCaag                                                                                             >  1:1294771/1‑98 (MQ=255)
         aTCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAATTTTGCACCg                                                                                            <  2:2027484/100‑1 (MQ=255)
          tgcaggtctctTGTTCCGTTCCCAGGTTTATATTTTCTTTATCGCCATACCCGATGCGTTCCGTTCCGTCCTTTTTCCCACGGTGCAAAATTTGCACCgt                                                                                           <  1:3601398/93‑1 (MQ=255)
           ccAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAATTTTGCACCgtg                                                                                          <  1:306461/100‑2 (MQ=255)
            cAGAGCTCTTGTTCCGTTCCCGGGTCGATATTGTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAATTTTGCACCgtgg                                                                                         <  1:176705/100‑3 (MQ=255)
            cAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAATTTTGCACCgtgg                                                                                         <  1:142082/100‑3 (MQ=255)
            cAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAATTTTGCACCgtgg                                                                                         <  1:3666074/100‑3 (MQ=255)
            cAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCgtag                                                                                         >  2:2318286/1‑100 (MQ=255)
               aaCTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTCGCACCGTAGTaa                                                                                      <  2:2889142/100‑1 (MQ=255)
                aCTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTaaa                                                                                     >  2:2999040/1‑100 (MQ=255)
                 ctctTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAg                                                                                    >  2:1889548/1‑100 (MQ=255)
                        tccgttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTttgtt                                                                             >  2:1006617/1‑100 (MQ=255)
                           gttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGcc                                                                          <  2:1301131/100‑1 (MQ=255)
                           gttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTCTCCCACGGTGTAAAATTTGCACCGTAGTTAAGTTttgttgtc                                                                          <  2:2971580/100‑3 (MQ=255)
                             tccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGcccc                                                                        <  2:1350761/100‑1 (MQ=255)
                             tccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGcccc                                                                        >  2:1072637/1‑100 (MQ=255)
                             tccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGcccc                                                                        <  1:2006627/100‑1 (MQ=255)
                              cccAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGccccc                                                                       <  2:2878369/100‑1 (MQ=255)
                                   gTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGc                                                                  <  1:4078433/100‑1 (MQ=255)
                                   gTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTACACCGTAGTAAAGTTTTGTTGCCCCCAACGc                                                                  >  1:622626/1‑100 (MQ=255)
                                     cGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAg                                                                <  2:3186809/100‑1 (MQ=255)
                                     cGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAg                                                                <  2:2007841/100‑1 (MQ=255)
                                        tatTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTa                                                              >  1:2677699/1‑99 (MQ=255)
                                           tttCTTTATCGCCATACCAGATGCGTTCCGTTTTGACCTTTTTCCCACGGTGCAAAATTTGCACCCTAATAAAGTTTTGTTGCCCCCAACACAGTCAt                                                            >  1:4158954/1‑98 (MQ=255)
                                            ttCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAAtttt                                                         >  1:3713899/1‑100 (MQ=255)
                                              cTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCt                                                       <  1:2666384/100‑1 (MQ=255)
                                               gttAGCGCCATACCAAATGCGTTCCGTTCCGACCTGTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTTGCCCCAACGCAGTAATTTTc                                                        <  1:1759562/97‑1 (MQ=255)
                                                ttATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTg                                                     <  2:3392879/100‑1 (MQ=255)
                                                ttATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTg                                                     <  1:2482416/100‑1 (MQ=255)
                                                 tATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGc                                                    >  1:91291/1‑100 (MQ=255)
                                                  atctccATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGcc                                                   <  1:954762/96‑1 (MQ=255)
                                                  aTCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGcc                                                   <  2:504714/100‑1 (MQ=255)
                                                  aTCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGcc                                                   <  2:2755736/100‑1 (MQ=255)
                                                    cGCCATACCATATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAATTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCctc                                                 >  1:1482459/1‑100 (MQ=255)
                                                    cGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCctc                                                 <  2:3968490/100‑1 (MQ=255)
                                                     gCCATACCAGATGCGTTCCGTTCCGACCTTTTTTTTTTTTTTTTAAATTTGCACCGTACTACAGTTTTGTTGCCCCCAACGCAGTAATTTTCtt                                                      >  1:3775307/1‑94 (MQ=255)
                                                     gCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCctct                                                >  2:2917814/1‑100 (MQ=255)
                                                     gCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCctct                                                >  2:2300774/1‑100 (MQ=255)
                                                      ccATACCAGCTGCGTTCCGTGCCGACCTTTTTCCCCCGTTTCAAAATTGGCACCGTAGTAAAGTTTTGTTCCCCCCAACGCAGTAATTTTCTTccc                                                   <  1:4078211/96‑1 (MQ=255)
                                                      ccATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGccagat                                               >  2:954766/1‑96 (MQ=255)
                                                      ccATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCtt                                               >  1:3291812/1‑100 (MQ=255)
                                                       cATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTc                                              >  2:2757364/1‑100 (MQ=255)
                                                       cATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTc                                              >  1:3625884/1‑100 (MQ=255)
                                                        aTACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTcc                                             <  2:4198455/100‑1 (MQ=255)
                                                           ccAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCGCCAACGCAGTAATTTTCTTGCCTCTTCCTCa                                          >  1:2969802/1‑100 (MQ=255)
                                                           ccAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCa                                          <  2:1831786/100‑1 (MQ=255)
                                                           ccAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCa                                          <  2:3625905/100‑1 (MQ=255)
                                                            cAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCa                                          <  1:627874/99‑1 (MQ=255)
                                                             aGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACg                                        >  2:1050907/1‑100 (MQ=255)
                                                                tGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACTCAGTCATTTTCTTGCCTCTTCCTCACGccc                                     >  1:4266139/1‑100 (MQ=255)
                                                                tGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGAc                                      >  1:1086988/1‑99 (MQ=255)
                                                                  cgttccgttcCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCtt                                   >  2:4277952/1‑100 (MQ=255)
                                                                     tccgttccgACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTg                                <  1:3315255/100‑1 (MQ=255)
                                                                       cgttccgACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa                              >  2:3863563/1‑100 (MQ=255)
                                                                          tccgACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTg                           >  2:1511745/1‑100 (MQ=255)
                                                                            cgACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTc                         >  2:2344032/1‑100 (MQ=255)
                                                                            cgACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTc                         >  2:3523646/1‑100 (MQ=255)
                                                                            cgACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTc                         <  2:3443719/100‑1 (MQ=255)
                                                                            cgACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTc                         <  2:1057977/100‑1 (MQ=255)
                                                                             gACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCa                        >  2:1260887/1‑100 (MQ=255)
                                                                              aCCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAg                       <  2:531257/100‑1 (MQ=255)
                                                                                cTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGcc                     <  2:78040/100‑1 (MQ=255)
                                                                                  ttttCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGcccc                   <  2:2400262/100‑1 (MQ=255)
                                                                                    ttCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCa                 <  2:871603/100‑1 (MQ=255)
                                                                                     tCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCAt                >  2:2918866/1‑100 (MQ=255)
                                                                                      cccACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCAtt               <  2:1472500/100‑1 (MQ=255)
                                                                                      cccACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCAtt               <  2:1022530/100‑1 (MQ=255)
                                                                                      cccACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCAtt               >  2:865046/1‑100 (MQ=255)
                                                                                      actaCGGTGCAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCAtt               >  2:604208/4‑100 (MQ=255)
                                                                                      actaCGGTGCAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCAtt               >  2:1745374/4‑100 (MQ=255)
                                                                                       ctaCGGTGCACATTTTGCACCGTAGTAAAGTTTTGTTGCTCCTAACGCAGTAATTTTCTTGCCTCTTCTTCACGACCTTTTGCACTGTCAGCCCCCATTa              >  1:1906023/3‑100 (MQ=255)
                                                                                       ctaCGGTGCAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCAtt               >  2:3638930/3‑99 (MQ=255)
                                                                                       ctaCGGTGCAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTa              >  2:3965189/3‑100 (MQ=255)
                                                                                       ctaCGGTGCAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTa              >  2:1990669/3‑100 (MQ=255)
                                                                                       ccACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTa              <  1:2980062/100‑1 (MQ=255)
                                                                                       ccACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTa              >  2:3382334/1‑100 (MQ=255)
                                                                                        taCGGTGCAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTAc             >  2:3914062/2‑100 (MQ=255)
                                                                                        cACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACGGTCAGCCCCCATTAc             >  2:4328769/1‑100 (MQ=255)
                                                                                         aCGGTGCAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTAcc            >  1:4153129/1‑100 (MQ=255)
                                                                                         aCGGTGCAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTAcc            >  2:1148686/1‑100 (MQ=255)
                                                                                         aCGGTGCAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTAcc            >  2:797292/1‑100 (MQ=255)
                                                                                          cGGTGCAACATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCa           <  1:3485302/100‑1 (MQ=255)
                                                                                          cGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCa           <  2:1043259/100‑1 (MQ=255)
                                                                                           ggTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTGCTCACGACCTTTTGCACTGTCAGCCCCCATTACCAc          <  2:3110222/100‑1 (MQ=255)
                                                                                            gTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACt         >  2:2423957/1‑100 (MQ=255)
                                                                                             tGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTg        <  2:2159435/100‑1 (MQ=255)
                                                                                              gCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGc       <  2:3499968/100‑1 (MQ=255)
                                                                                               cAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCa      >  2:1648665/1‑100 (MQ=255)
                                                                                               cAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCa      >  1:3844920/1‑100 (MQ=255)
                                                                                                  aaTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATg   <  1:1006613/100‑1 (MQ=255)
                                                                                                   aTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATga  <  2:1942457/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
GGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGA  >  NC_000913/2081589‑2081787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: