Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,314,801 G→T 17.3% Q438H (CAG→CAT) ‡ rcsD → phosphotransfer intermediate protein in two‑component regulatory system with RcsBC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,314,8010GT17.3% 493.4 / 87.8 191Q438H (CAG→CATrcsDphosphotransfer intermediate protein in two‑component regulatory system with RcsBC
Reads supporting (aligned to +/- strand):  ref base G (76/82);  new base T (23/10);  total (99/92)
Fisher's exact test for biased strand distribution p-value = 3.42e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01

Only 100 of 198 total aligned reads displayed.

GGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGAT  >  NC_000913/2314702‑2314900
                                                                                                   |                                                                                                   
ggggATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAg                                                                                                     <  1:89741/100‑1 (MQ=255)
 gggATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAgg                                                                                                    >  1:27093/1‑100 (MQ=255)
  ggATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGa                                                                                                   <  2:1698284/100‑1 (MQ=255)
     ttattCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATcg                                                                                                <  2:3202022/100‑1 (MQ=255)
      tattCCGGCGACGATCAATAACGAGCTGTATGAGATCCGCCTGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTACATTATTCGCGATCAGGATcg                                                                                                >  1:3828954/1‑99 (MQ=255)
      tattCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATcgc                                                                                               <  1:3404732/100‑1 (MQ=255)
       attCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCAAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATcgcg                                                                                              >  1:1957216/1‑100 (MQ=255)
        ttCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGa                                                                                             <  2:4255377/100‑1 (MQ=255)
        ttCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGa                                                                                             <  1:2320435/100‑1 (MQ=255)
           aGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGt                                                                                          <  1:1389034/100‑1 (MQ=255)
            ggCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTg                                                                                         >  2:3006025/1‑100 (MQ=255)
            ggCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTg                                                                                         >  2:3997339/1‑100 (MQ=255)
             gCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCCTGATCGCGaataat                                                                                        <  2:1263955/100‑5 (MQ=255)
              cgacgaTCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCCTGATCGCGaataatg                                                                                       <  2:2706633/100‑6 (MQ=255)
              cgacgaTCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCCTGATCGCGaataatg                                                                                       <  1:1168598/100‑6 (MQ=255)
              cgacgaTCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCt                                                                                       <  2:4186297/100‑1 (MQ=255)
              cgacgaTCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCt                                                                                       <  1:1363377/100‑1 (MQ=255)
               gacgaTCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTg                                                                                      <  1:2980043/100‑1 (MQ=255)
                acgaTCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCCTGATCGCGaataatgaa                                                                                     <  2:929018/100‑8 (MQ=38)
                 cgaTCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGt                                                                                    <  2:2956522/100‑1 (MQ=255)
                 cgaTCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGt                                                                                    >  2:3468709/1‑100 (MQ=255)
                  gaTCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTa                                                                                   >  2:3062912/1‑100 (MQ=255)
                  gaTCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTa                                                                                   >  2:1572417/1‑100 (MQ=255)
                   aTCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTaa                                                                                  >  2:3847468/1‑100 (MQ=255)
                     cAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAAc                                                                                >  1:641823/1‑100 (MQ=255)
                      aaTAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACa                                                                               >  2:2085380/1‑100 (MQ=255)
                      aaTAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACa                                                                               <  2:3699244/100‑1 (MQ=255)
                      aaTAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACa                                                                               <  2:1187611/100‑1 (MQ=255)
                      aaTAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACa                                                                               <  2:817400/100‑1 (MQ=255)
                      aaTAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGATAAACa                                                                               >  1:2157664/1‑100 (MQ=255)
                       aTAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACaa                                                                              <  1:1611131/100‑1 (MQ=255)
                        tAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAg                                                                             <  1:1884183/100‑1 (MQ=255)
                         aaCGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGa                                                                            <  2:1610464/100‑1 (MQ=255)
                         aaCGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGa                                                                            >  2:3236692/1‑100 (MQ=255)
                           cGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGCTCGCGAAGTGCTGGTAAACAAGaaa                                                                          >  1:3725887/1‑100 (MQ=255)
                              gCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTc                                                                       <  1:1035294/100‑1 (MQ=255)
                               cTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCa                                                                      <  2:2674373/100‑1 (MQ=255)
                                 gTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAg                                                                    <  1:2857709/100‑1 (MQ=255)
                                 gTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAg                                                                    >  2:4200379/1‑100 (MQ=255)
                                 gTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAg                                                                    <  1:9826/100‑1 (MQ=255)
                                   aTGAGATCCGCATGTTCCGCAGCCAGGTCGCGCGGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCa                                                                  >  1:2885695/1‑100 (MQ=255)
                                   aTGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGAGCTGGTAAACAAGAAACTCAAGCa                                                                  >  1:3585891/1‑100 (MQ=255)
                                     gagaTCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAgg                                                                >  1:2367543/1‑100 (MQ=255)
                                      agaTCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGCTCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCa                                                                  >  1:4292674/1‑97 (MQ=255)
                                      agaTCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGgc                                                               >  1:2794227/1‑100 (MQ=255)
                                       gaTCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGgcg                                                              >  2:4014549/1‑100 (MQ=255)
                                        aTCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGgcgc                                                             <  1:665348/100‑1 (MQ=255)
                                         tCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCa                                                            >  2:390507/1‑100 (MQ=255)
                                         tCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCa                                                            >  1:708042/1‑100 (MQ=255)
                                           cGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGc                                                          <  2:25269/100‑1 (MQ=255)
                                                gTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGGAGCGTCTg                                                     <  1:880803/100‑1 (MQ=255)
                                                gTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTg                                                     <  2:754454/100‑1 (MQ=255)
                                                gTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTg                                                     >  1:1900983/1‑100 (MQ=255)
                                                 ttCCGCCGCAAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGAACAGGATCGCGAACTGCTGGTAAAAGAGAAACTCAAGCAGGCGCAGCGGCTGt                                                    >  1:3899479/1‑100 (MQ=255)
                                                  tCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTa                                                   <  2:3893406/100‑1 (MQ=255)
                                                  tCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTa                                                   <  2:2183496/100‑1 (MQ=255)
                                                  tCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTa                                                   <  1:2249809/100‑1 (MQ=255)
                                                   cctcAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTAt                                                  <  1:1999131/97‑1 (MQ=255)
                                                   ccGCAGCCGGGTCGCGCCGCGCACACGAATTTTCATTGCTCGCGAGAAGGATTGCGAAGTGCTGGTAAACAAGACATTCAAACAGGCGCAGCGCCTGTAt                                                  >  1:2145962/1‑100 (MQ=255)
                                                    cGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATg                                                 <  2:2222014/100‑1 (MQ=255)
                                                       aGCCAGGTCGCGCCGCGCACATAAATTTTCATTATGCCGGATCAGGGTCGCGAAGTGCTGTTAAACAAGAATCTCAAGCAGGAGCAGCGTCTGTATg                                                 <  1:2922947/97‑1 (MQ=255)
                                                        gCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGaa                                             <  1:4338158/100‑1 (MQ=255)
                                                           aGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGaaaaa                                          >  2:1908496/1‑100 (MQ=255)
                                                             gTCGCGCCGCGCACACAAATTTTCATTATTCGCGATAAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAAcc                                        >  2:1087862/1‑100 (MQ=255)
                                                                gcgccgcgcACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACcagc                                     <  2:1766081/100‑1 (MQ=255)
                                                                gcgccgcgcACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACcagc                                     <  2:3947695/100‑1 (MQ=255)
                                                                gcgccgcgcACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATCAGAAAAACcagc                                     <  2:2888774/100‑1 (MQ=255)
                                                                 cgccgcgcACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACcagca                                    <  1:1087858/100‑1 (MQ=255)
                                                                   ccgcgcACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAgg                                  >  2:4067038/1‑100 (MQ=255)
                                                                    cgcgcACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAggg                                 >  1:3629691/1‑100 (MQ=255)
                                                                     gcgcACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAgggg                                <  2:3502734/100‑1 (MQ=255)
                                                                       gcACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCg                              <  2:666767/100‑1 (MQ=255)
                                                                       gcACACAAATTTTCATTATTCGCAATCAGGATCGCTAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGc                               <  1:4189358/99‑1 (MQ=255)
                                                                         acacaAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGga                            <  1:2461715/100‑1 (MQ=255)
                                                                          cacaAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGgat                           >  2:232968/1‑100 (MQ=255)
                                                                            caAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGgatga                         >  2:1843747/1‑100 (MQ=255)
                                                                            caAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGgatga                         >  2:3085823/1‑100 (MQ=255)
                                                                                   tCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTAt                  >  2:4323388/1‑100 (MQ=255)
                                                                                   tCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTAt                  >  1:3909184/1‑100 (MQ=255)
                                                                                    cATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATg                 <  2:1733422/100‑1 (MQ=255)
                                                                                    cATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATg                 <  1:344230/100‑1 (MQ=255)
                                                                                     gcacttCGCGATCCTGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGa                >  1:2212385/5‑100 (MQ=255)
                                                                                     attattCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGTGGATGATCTTTATGa                >  2:4331683/1‑100 (MQ=255)
                                                                                      ttattCGCGATCAGGATCGCAAAGTGCTGGTAATCAAGAAACTCAAGAAGGCGGAGCGTCTGTAGGAGAGAGACCAGCCGGGGCGGTTGATCTCTATGaa               >  1:4071366/1‑100 (MQ=255)
                                                                                      cacttCGCGATCCTGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGaa               >  2:259713/4‑100 (MQ=255)
                                                                                        cttCGCGATCCTGATCGCGAAGTGCTGGTAAACAAGAATCTCAAGCAGGCGGAGCGTCTGTAAGAGAAAAACCAGAAGGGGGGGATGATCTTTATg                 >  1:2777706/2‑96 (MQ=255)
                                                                                        cttCGCGATCCTGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGaaaa             >  2:2808111/2‑100 (MQ=255)
                                                                                        cttCGCGATCCTGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGaaaa             >  2:519274/2‑100 (MQ=255)
                                                                                        cttCGCGATCCTGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGaaaa             >  2:343630/2‑100 (MQ=255)
                                                                                        cttCGCGATCCTGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGaaaa             >  2:3019018/2‑100 (MQ=255)
                                                                                        cttCGCGATCCTGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGaa               >  1:1571758/2‑98 (MQ=255)
                                                                                         ttCGCGATCCTGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGaaaaa            >  2:3566164/1‑100 (MQ=255)
                                                                                          tCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAAc           <  2:970102/100‑1 (MQ=255)
                                                                                           cgcgATGAGGATCGCGAAGTGATGGTAAAAAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGaaaaa            <  1:4242456/98‑1 (MQ=255)
                                                                                           cgcgATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACa          >  2:2734604/1‑100 (MQ=255)
                                                                                            gcgATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACAt         >  1:2675934/1‑100 (MQ=255)
                                                                                              gATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTg       >  1:3704774/1‑100 (MQ=255)
                                                                                              gATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTg       >  1:460928/1‑100 (MQ=255)
                                                                                                tCAGGATCGCGAAGTGCTGGTAAACAAGAATCTCAAGGAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGc     <  1:2085372/100‑1 (MQ=255)
                                                                                                tCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGc     <  1:803557/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
GGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGAT  >  NC_000913/2314702‑2314900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: