Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,434,094 G→A 15.4% R216R (CGC→CGT dedA ← DedA family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,434,0940GA15.4% 439.7 / 52.2 179R216R (CGC→CGTdedADedA family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (74/76);  new base A (12/16);  total (87/92)
Fisher's exact test for biased strand distribution p-value = 5.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 188 total aligned reads displayed.

GCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAA  >  NC_000913/2433995‑2434191
                                                                                                   |                                                                                                 
gCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTgcggcg                                                                                                   <  2:1637471/100‑1 (MQ=255)
  ccccAACAGACCATGAATGTTGCACATTAAAACATACCAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGcgcg                                                                                                 >  2:3096737/1‑100 (MQ=255)
  ccccAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGcgcg                                                                                                 <  1:4252114/100‑1 (MQ=255)
    ccAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTg                                                                                               <  1:4140458/100‑1 (MQ=255)
    ccAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTg                                                                                               <  1:3211393/100‑1 (MQ=255)
     cAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGc                                                                                              <  1:1936005/100‑1 (MQ=255)
      aaCAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGcc                                                                                             >  2:2392340/1‑100 (MQ=255)
         aGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCa                                                                                          <  1:1133661/100‑1 (MQ=255)
          gACCGTGAATGTTGCACATTAAAACATAAGAGCCCGAAACGTTGTATAAAAAATGGGGGGAGCCGCGTTGTTACTTTTTATTTTGCGTCGCGTGCCGCAg                                                                                         >  1:2563063/1‑100 (MQ=255)
          gACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAg                                                                                         >  1:1019220/1‑100 (MQ=255)
          gACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAg                                                                                         >  2:3737015/1‑100 (MQ=255)
            ccATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCACGCGCCGCaaaa                                                                                       <  1:3923452/100‑4 (MQ=255)
            ccATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCACGCGCCGCaaaa                                                                                       <  2:1200039/100‑4 (MQ=255)
             cATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAgcgc                                                                                      >  2:651202/1‑100 (MQ=255)
             cATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCACGCGCCGCaaaat                                                                                      <  2:2862011/100‑5 (MQ=255)
              aTGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGTACGCGCCGCaaaat                                                                                      <  1:1738510/99‑5 (MQ=255)
              aTGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGcgcg                                                                                     <  1:4055439/100‑1 (MQ=255)
              aTGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCACGCGCCGCaaaata                                                                                     <  2:1748576/100‑6 (MQ=255)
              aTGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCACGCGCCGCaaaat                                                                                      <  1:2023320/99‑5 (MQ=255)
                gAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGtt                                                                                   <  2:1682465/100‑1 (MQ=255)
                  aTGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTtg                                                                                 <  1:921785/100‑1 (MQ=255)
                  aTGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTtg                                                                                 <  1:3257829/100‑1 (MQ=255)
                    gTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTtgtg                                                                               <  1:2743537/100‑1 (MQ=255)
                      tGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGAc                                                                             >  2:2790247/1‑100 (MQ=255)
                        cacaTTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACgg                                                                           >  1:743047/1‑100 (MQ=255)
                        cacaTTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACgg                                                                           <  1:3722772/100‑1 (MQ=255)
                            ttAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGatta                                                                       >  1:3551485/1‑100 (MQ=255)
                             tAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGattat                                                                      >  1:2606167/1‑100 (MQ=255)
                              aaaaCATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGattatt                                                                     >  2:2213941/1‑100 (MQ=255)
                               aaaCATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTAttt                                                                    >  2:1049193/1‑100 (MQ=255)
                               aaaCATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTAttt                                                                    <  1:2220779/100‑1 (MQ=255)
                                aaCATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTc                                                                   >  2:583670/1‑100 (MQ=255)
                                aaCATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTc                                                                   <  1:2473010/100‑1 (MQ=255)
                                  cATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGa                                                                 <  1:2845150/100‑1 (MQ=255)
                                  cATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGa                                                                 <  2:2771544/100‑1 (MQ=255)
                                   aTAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGAt                                                                <  2:3541486/100‑1 (MQ=255)
                                    tAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATg                                                               <  1:4108013/100‑1 (MQ=255)
                                     aaCAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGa                                                              >  2:2941420/1‑100 (MQ=255)
                                      aCAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGAc                                                             <  2:3581016/100‑1 (MQ=255)
                                       cAGCCCGAAACTTTGTATAAAAAAGTGGTCGACCCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATg                                                               <  1:1036840/97‑1 (MQ=255)
                                         gCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGcc                                                          <  2:2915563/100‑1 (MQ=255)
                                          cccGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGccc                                                         >  2:2098720/1‑100 (MQ=255)
                                           ccGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCg                                                        >  2:497580/1‑100 (MQ=255)
                                           ccGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCg                                                        >  1:2246628/1‑100 (MQ=255)
                                           ccGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCg                                                        >  2:1977895/1‑100 (MQ=255)
                                               aaCTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCaa                                                    <  1:3095694/100‑1 (MQ=255)
                                                 cTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACAGATTATTTCGATGACGCCCGGCaaaa                                                  <  1:3976776/100‑1 (MQ=255)
                                                  tttGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAAt                                                 >  2:686291/1‑100 (MQ=255)
                                                  tttGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAAt                                                 <  1:3488014/100‑1 (MQ=255)
                                                    tGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAg                                               >  1:2986401/1‑100 (MQ=255)
                                                       aTAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGaaa                                            >  2:3286997/1‑100 (MQ=255)
                                                        tAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAAc                                           >  2:859947/1‑100 (MQ=255)
                                                        tAAAAAAGTGGTCGAACCGCGGAGATACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGATTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAAc                                           >  1:1017004/1‑100 (MQ=255)
                                                         aaaaaaGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAAcc                                          >  2:4217775/1‑100 (MQ=255)
                                                            aaaGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACcaca                                       <  1:3676177/100‑1 (MQ=255)
                                                             aaggagTCGCACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTATGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAACCcac                                        <  1:4164959/93‑1 (MQ=255)
                                                             aaGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCACACTAGAACCCACaa                                      >  2:1462591/1‑100 (MQ=255)
                                                              aGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACaat                                     <  2:3800970/100‑1 (MQ=255)
                                                              aGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACaa                                      >  1:2639996/1‑99 (MQ=255)
                                                                 ggTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACaataat                                  <  1:3590821/100‑1 (MQ=255)
                                                                  gTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATc                                 <  1:1355480/100‑1 (MQ=255)
                                                                   tCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATcc                                <  2:4076791/100‑1 (MQ=255)
                                                                    cGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGATTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATcc                                >  1:2195256/1‑99 (MQ=255)
                                                                     gAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCcg                              >  1:521712/1‑100 (MQ=255)
                                                                     gAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCcg                              >  1:969184/1‑100 (MQ=255)
                                                                      aaCCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCcga                             >  2:776007/1‑100 (MQ=255)
                                                                        ncgcgGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCcgacg                           >  1:979686/2‑100 (MQ=255)
                                                                        ccGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCcgacg                           >  1:3035113/1‑100 (MQ=255)
                                                                        ccGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCcgacg                           >  2:2909624/1‑100 (MQ=255)
                                                                        ccGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCcgac                            >  1:713057/1‑99 (MQ=255)
                                                                          gcgGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGAt                         >  2:2005500/1‑100 (MQ=255)
                                                                           cgGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATc                        <  1:4278456/100‑1 (MQ=255)
                                                                           cgGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATc                        <  1:484384/100‑1 (MQ=255)
                                                                           cgGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATc                        <  2:4259337/100‑1 (MQ=255)
                                                                             gAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATc                        >  1:1579453/1‑98 (MQ=255)
                                                                              aGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATcagc                     <  2:978710/100‑1 (MQ=255)
                                                                               gTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATcagca                    >  1:115961/1‑100 (MQ=255)
                                                                                 tACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCagcagc                  <  2:1328922/100‑1 (MQ=255)
                                                                                    caaacgcgctGCGGCACGCGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTa               >  2:2506790/10‑100 (MQ=255)
                                                                                    caaacgcgctGCGGCACGCGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTa               >  2:930397/10‑100 (MQ=255)
                                                                                    caaacgcgctGCGGCACGCGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTa               >  2:3648369/10‑100 (MQ=255)
                                                                                    caaacgcgctGCGGCACGCGCCGCAGCGCGTTTGTGACGGATTATTCCGATGACGCCCGGTCAAATAGAAACCACAATAATACCGACGATCAGGAGCTTa               >  1:2928027/10‑100 (MQ=255)
                                                                                      tttATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAg             <  2:1522725/100‑1 (MQ=255)
                                                                                       ttATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAgg            <  1:2104233/100‑1 (MQ=255)
                                                                                        tATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGt           <  2:1521615/100‑1 (MQ=255)
                                                                                         gcgctGCGGCACGCGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGtt          >  2:3632757/5‑100 (MQ=255)
                                                                                          ttttGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTa         >  2:2331606/1‑100 (MQ=255)
                                                                                          cgctGCGGCACGCGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTa         >  2:2235022/4‑100 (MQ=255)
                                                                                          cgctGCGGCACGCGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTa         >  1:1635249/4‑100 (MQ=255)
                                                                                          cgctGCGGCACGCGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTa         >  1:1534217/4‑100 (MQ=255)
                                                                                          cgctGCGGCACGCGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTa         >  1:1205124/4‑100 (MQ=255)
                                                                                          cgctGCGGCACGCGCCGCAACACGATAGTAACGGATTAGTTCGATGACGCCCGGCAAAATACAAACCACAATAATCCCCACGATCAGCAGCTTaatg            >  1:1488342/4‑95 (MQ=255)
                                                                                             tGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATcc      <  2:3833985/100‑1 (MQ=255)
                                                                                             tGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATcc      >  1:3714678/1‑100 (MQ=255)
                                                                                             tGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATcc      <  2:3533800/100‑1 (MQ=255)
                                                                                             tGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATcc      <  1:2281408/100‑1 (MQ=255)
                                                                                             tGCGGCGCGTGCCGCAGCGCGTTCGCGACGGATTATTTCGATGACGCCCGGCAAAATAGAATCCACAATAATCCCGACGATCAGCAGCTTAAGGTTATcc      <  1:850548/100‑1 (MQ=255)
                                                                                             tGCGGCGCGTGCCGCAGCGCGGTTTTGACGGATTATTTCGATGACACCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATcc      <  1:2413878/100‑1 (MQ=255)
                                                                                              gcggcgCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCt     <  2:2434154/100‑1 (MQ=255)
                                                                                               cggcgCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTg    >  2:3910951/1‑100 (MQ=255)
                                                                                                   |                                                                                                 
GCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAA  >  NC_000913/2433995‑2434191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: