Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,731,073 A→T 15.1% noncoding (85/1542 nt) rrsG ← 16S ribosomal RNA of rrnG operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,731,0730AT15.1% 412.3 / 58.2 159noncoding (85/1542 nt)rrsG16S ribosomal RNA of rrnG operon
Reads supporting (aligned to +/- strand):  ref base A (81/54);  new base T (7/17);  total (88/71)
Fisher's exact test for biased strand distribution p-value = 6.97e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.35e-01

Only 100 of 174 total aligned reads displayed.

CATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTC  >  NC_000913/2730974‑2731172
                                                                                                   |                                                                                                   
cATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAgcagca                                                                                                     >  2:1382952/1‑100 (MQ=40)
 aTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCaa                                                                                                    <  2:3450473/100‑1 (MQ=40)
 aTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCaa                                                                                                    >  2:1229983/1‑100 (MQ=40)
   tATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAgc                                                                                                  >  2:3545014/1‑100 (MQ=38)
     tGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAgctg                                                                                                >  2:2186202/1‑100 (MQ=38)
          tATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTcc                                                                                           <  2:3846965/100‑1 (MQ=255)
           aTTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCTTGCTGCTTcgc                                                                                          <  1:1072620/100‑3 (MQ=255)
           aTTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCTTGCTGCTTcgc                                                                                          <  2:117450/100‑3 (MQ=255)
            ttAGCTACCGTTTCCAGTAGTTATCCCCCTCCCTCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCTTGCTGCTTcgct                                                                                         <  1:1559891/100‑4 (MQ=14)
            ttAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCTTGCTGCTTcgct                                                                                         <  2:3483436/100‑4 (MQ=255)
            ttAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCTTGCTGCTTcgct                                                                                         <  2:3355302/100‑4 (MQ=14)
            ttAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCTTGCTGCTTcgct                                                                                         <  2:1638881/100‑4 (MQ=14)
             tAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCTTGCTGCTTcgctg                                                                                        <  2:886602/100‑5 (MQ=14)
              aGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGGAAGCAGCTTGCTGCTTcgctgac                                                                                      <  1:485095/100‑7 (MQ=14)
               gCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTa                                                                                      >  2:566625/1‑100 (MQ=255)
                cTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCTTGCTGCTTcgctgacg                                                                                     <  2:455519/100‑8 (MQ=14)
                 tagcGTTTCCAGTAGTTATCCCCCTCCATCAGGTAGCGTCCCAGACACTACTCACCCGTCCGCAGCTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTAc                                                                                     <  2:1447472/96‑1 (MQ=14)
                 tACCGTTTCCAGTAGTTATCCCTCTACTTCAGGCAGCTTCCCAGACATTAGTCACTCGTCCGCCGCTCGTCATCGAAGCAGCAAGCTGCTTCCTg                                                                                         <  1:915657/95‑1 (MQ=14)
                 tACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGGAAGCAGCTTGCTGCTTcgctgacgag                                                                                   <  1:295825/100‑10 (MQ=14)
                  aCCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCg                                                                                   >  2:1619414/1‑100 (MQ=40)
                        tcgagtagtTATCCCCCTCCATCAGGAAGCTTCCCAGAAATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGAc                                                                             >  1:1004240/4‑100 (MQ=255)
                        tCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGAc                                                                             <  2:1737468/100‑1 (MQ=255)
                         ccAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACt                                                                            <  1:192343/100‑1 (MQ=255)
                          cAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACtt                                                                           >  2:4086653/1‑100 (MQ=255)
                              agtTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCAt                                                                       >  1:178628/1‑100 (MQ=40)
                                ttATCCCCCTCCATCAGGCAGATTCCCAGACATTACCCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCAtg                                                                      <  1:2965387/99‑1 (MQ=255)
                                 tATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCAtgtg                                                                    >  1:3949499/1‑100 (MQ=40)
                                   tCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCAtgtg                                                                    <  1:4196611/98‑1 (MQ=40)
                                   tCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGtt                                                                  >  1:963117/1‑100 (MQ=255)
                                   tCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGtt                                                                  >  1:3922099/1‑100 (MQ=40)
                                   tCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGtt                                                                  >  2:2668874/1‑100 (MQ=255)
                                   tCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGtt                                                                  <  2:2881858/100‑1 (MQ=255)
                                     ccccTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAg                                                                >  1:2571992/1‑100 (MQ=40)
                                     ccccTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAg                                                                >  2:1606222/1‑100 (MQ=255)
                                      cccTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAgg                                                               <  2:1746294/100‑1 (MQ=255)
                                      cccTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAgg                                                               <  2:1296215/100‑1 (MQ=40)
                                       ccTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCATCGAAGCAGCAAGCTGCTACCTGTTACTGTTCGACTTGCATGTGTTAg                                                                >  1:2976270/1‑98 (MQ=255)
                                       ccTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGc                                                              <  1:148861/100‑1 (MQ=255)
                                        cTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGcc                                                             <  2:3940644/100‑1 (MQ=255)
                                          ccATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTg                                                           >  1:677449/1‑100 (MQ=255)
                                           caccAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTgc                                                          >  2:2766073/4‑100 (MQ=255)
                                            aTCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTgcc                                                         <  2:1231777/100‑1 (MQ=255)
                                             tCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTgccg                                                        >  1:4089211/1‑100 (MQ=40)
                                             tCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTCCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTgccg                                                        <  1:2360824/100‑1 (MQ=255)
                                                ggCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCa                                                     >  2:1956584/1‑100 (MQ=255)
                                                 gCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAg                                                    <  2:1615534/100‑1 (MQ=40)
                                                  cAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGGCTTGCATGTGTTAGGCCTGCCGCCAg                                                    <  1:129007/99‑1 (MQ=255)
                                                   aGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCg                                                  <  1:2286104/100‑1 (MQ=255)
                                                    ggttCCCAGACATTACTCACCCGTCCGCTACTCGTCAGCGAAGCAGAAAGCTGCTTCCTGTTACCGTTCGACTTGCATGAGTTAGGCCTGCCGCCAGCGt                                                 >  1:3439426/3‑100 (MQ=11)
                                                    gCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGt                                                 <  1:3345669/100‑1 (MQ=40)
                                                    gCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGt                                                 >  2:1356136/1‑100 (MQ=40)
                                                    gCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGt                                                 <  2:2707477/100‑1 (MQ=40)
                                                      ttCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTc                                               >  1:3051702/1‑100 (MQ=38)
                                                       tCCCAGACATTACTCACCCGTCCGCCACTCGTTAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCa                                              >  2:4174519/1‑100 (MQ=255)
                                                       tCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCa                                              >  2:4278139/1‑100 (MQ=38)
                                                        cccAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCaa                                             >  2:2075369/1‑100 (MQ=35)
                                                              cATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTTTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTg                                         <  1:1418790/98‑1 (MQ=38)
                                                              cATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAg                                       <  2:3637127/100‑1 (MQ=38)
                                                              cATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAg                                       <  2:2031068/100‑1 (MQ=38)
                                                              cATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAg                                       <  1:2807242/100‑1 (MQ=38)
                                                               aTTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGc                                      >  2:2564325/1‑100 (MQ=38)
                                                                ttACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGcc                                     >  1:1922648/1‑100 (MQ=38)
                                                                ttACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGcc                                     >  1:357350/1‑100 (MQ=38)
                                                                ttACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGcc                                     <  1:2668861/100‑1 (MQ=38)
                                                                ttACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGc                                      <  1:839619/99‑1 (MQ=35)
                                                                 tACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGGCTGTTAGGGGTTAGGCCGGCCGCCAGCGTTCAATCTGGGcc                                     >  2:3021980/1‑99 (MQ=11)
                                                                  aCTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCAt                                   >  1:4008701/1‑100 (MQ=38)
                                                                   cTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATg                                  >  1:2465928/1‑100 (MQ=38)
                                                                   cTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATg                                  >  1:61489/1‑100 (MQ=38)
                                                                   cTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATg                                  >  2:731171/1‑100 (MQ=38)
                                                                       cccGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCa                              >  1:1153653/1‑100 (MQ=35)
                                                                       cccGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCa                              >  2:2322008/1‑100 (MQ=35)
                                                                       cccGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCa                              >  2:1749578/1‑100 (MQ=38)
                                                                       cccGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCa                              >  1:1619099/1‑100 (MQ=38)
                                                                          gTCCGCCGCTGGTCAGCGAAGCAGCAAGCTGCTTCCTGTTGCCGTTCGACTTGCATGTGTGAGGCCTGCCGCCAGCGTTCAGTCTGAGCCATGATCAAAc                           <  1:407850/100‑1 (MQ=11)
                                                                            ccGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAActc                         <  1:3661580/100‑1 (MQ=38)
                                                                             cGCCACTCGTCAGCGAAGCAGCAAGCTTCTTCCTGTTACCGTTAGCCGTGCATGTGTTAGGCCCCCCTCCAGCGTTCAATCTGAGCCCTGATCAAActc                         >  1:3974605/1‑99 (MQ=11)
                                                                             cGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAActct                        >  2:2283648/1‑100 (MQ=35)
                                                                             cGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAActct                        >  2:2909535/1‑100 (MQ=38)
                                                                              gCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCtt                       >  2:510283/1‑100 (MQ=35)
                                                                                  cTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAAt                   <  2:2424282/100‑1 (MQ=38)
                                                                                  cTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAAt                   <  2:3750980/100‑1 (MQ=38)
                                                                                     gTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTa                >  2:2011782/1‑100 (MQ=38)
                                                                                       cAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTaaa              >  1:1302841/1‑100 (MQ=38)
                                                                                       acaggAAGCAGCTTGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTaaa              >  1:3169654/5‑100 (MQ=11)
                                                                                        caggAAGCAGCTTGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTaaaa             >  2:2043095/4‑100 (MQ=11)
                                                                                        caggAAGCAGCTTGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTaaaa             >  2:183532/4‑100 (MQ=255)
                                                                                        aGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTaaaa             <  2:218892/100‑1 (MQ=38)
                                                                                         aggAAGCAGCTTGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAg            >  2:1121903/3‑100 (MQ=255)
                                                                                          ggAAGCAGCTTGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGt           >  2:4184939/2‑100 (MQ=255)
                                                                                          cGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGt           >  1:3181089/1‑100 (MQ=38)
                                                                                          cGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGt           >  2:84174/1‑100 (MQ=38)
                                                                                           gAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGtt          >  1:155950/1‑100 (MQ=35)
                                                                                            aaGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGttt         <  1:3781/100‑1 (MQ=35)
                                                                                             agcagcAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTg        <  2:4008728/100‑1 (MQ=35)
                                                                                                agcaAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATg     >  2:62155/1‑100 (MQ=33)
                                                                                                 gcaAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATg     <  2:2385808/99‑1 (MQ=32)
                                                                                                  caAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTTTTAAATTGAAGAGTTTGAtcat   <  1:896882/100‑4 (MQ=11)
                                                                                                  caAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCt   >  2:594092/1‑100 (MQ=33)
                                                                                                   aaGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTTTTAAATTGAAGAGTTTGAtcatg  <  2:3376119/100‑5 (MQ=11)
                                                                                                   |                                                                                                   
CATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTC  >  NC_000913/2730974‑2731172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: