Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,761,620 T→G 16.7% K641Q (AAG→CAG)  yfjK ← radiation resistance protein; DEAD/H helicase‑like protein; CP4‑57 putative defective prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,761,6200TG16.7% 595.4 / 112.7 241K641Q (AAG→CAG) yfjKradiation resistance protein; DEAD/H helicase‑like protein; CP4‑57 putative defective prophage
Reads supporting (aligned to +/- strand):  ref base T (98/102);  new base G (24/16);  total (123/118)
Fisher's exact test for biased strand distribution p-value = 2.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 249 total aligned reads displayed.

GCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGC  >  NC_000913/2761522‑2761719
                                                                                                  |                                                                                                   
gCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCttt                                                                                                    >  2:1176091/1‑100 (MQ=255)
 ccAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTa                                                                                                   >  2:3997067/1‑100 (MQ=255)
 ccAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTa                                                                                                   <  2:1383337/100‑1 (MQ=255)
    aTCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAg                                                                                                >  1:4325937/1‑100 (MQ=255)
       tcttctAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCgt                                                                                             >  2:3572935/1‑100 (MQ=255)
        cttctAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCgtg                                                                                            >  2:2253076/1‑100 (MQ=255)
        cttctAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCgtg                                                                                            >  2:490314/1‑100 (MQ=255)
         ttctAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTGTAAAGCgtgg                                                                                           <  1:2383637/100‑1 (MQ=255)
          tctAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATGGACTTTGCTGAGTATACCACGCTTTACAGCgtgg                                                                                           >  1:3997066/1‑99 (MQ=255)
          tctAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCgtggt                                                                                          >  2:2829074/1‑100 (MQ=255)
           ctAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACACCgtggt                                                                                          >  2:2377972/1‑99 (MQ=255)
            tAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGgtgt                                                                                        >  1:2607623/1‑100 (MQ=255)
            tAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGgtgt                                                                                        <  2:81842/100‑1 (MQ=255)
            tAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTGTAAAGCgtggtat                                                                                        <  1:2633172/100‑3 (MQ=255)
            tAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTGTAAAGCgtggtan                                                                                        <  2:606215/100‑3 (MQ=255)
             aaGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGtt                                                                                       >  2:1364038/1‑100 (MQ=255)
              aGGCATTGATACCTGGTGGTAGATGGATACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGttt                                                                                      <  1:1342733/100‑1 (MQ=255)
              aGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTGTAAAGCgtggtatac                                                                                      <  1:70423/100‑5 (MQ=255)
              aGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGATGCGTATAGCACGCTTTACAGCGTGGTGttt                                                                                      >  1:2963299/1‑100 (MQ=255)
               ggCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTa                                                                                     >  1:2970450/1‑100 (MQ=255)
                gCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTGTAAAGCgtggtatacgc                                                                                    <  2:4391580/100‑7 (MQ=39)
                gCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTGTAAAGCgtggtatacgc                                                                                    <  2:3758517/100‑7 (MQ=39)
                gCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTGTAAAGCgtggtatacgc                                                                                    <  2:4189255/100‑7 (MQ=39)
                  aTTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACt                                                                                  <  2:3679880/100‑1 (MQ=255)
                  aTTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTGTAAAGCgtggtatacgcag                                                                                  <  2:1899060/100‑9 (MQ=38)
                   ttGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTa                                                                                 <  2:1072678/100‑1 (MQ=255)
                        acCTGGTGGTAGATGGAAACTTTCGAAAGCCAACTTGACATGTGTGTAATAGACTTTGCTGCGTATACGCCGGTCTACACCGTGGTGTTTAACTACAtc                                                                             >  1:4385550/1‑99 (MQ=255)
                         ccTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACAtcct                                                                           >  1:645180/1‑100 (MQ=255)
                         ccTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACAtcct                                                                           <  1:2349941/100‑1 (MQ=255)
                         ccTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACAtccc                                                                           >  1:2797414/1‑99 (MQ=255)
                            ggtggtAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCa                                                                        <  1:1985390/100‑1 (MQ=255)
                               ggtAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCa                                                                     >  1:614687/1‑100 (MQ=255)
                               ggtAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCa                                                                     <  2:2097640/100‑1 (MQ=255)
                                  aGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGc                                                                  >  1:3390394/1‑100 (MQ=255)
                                    aTGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTg                                                                <  1:3776003/100‑1 (MQ=255)
                                    aTGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTg                                                                <  2:3505900/100‑1 (MQ=255)
                                    aTGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTg                                                                >  1:2216249/1‑100 (MQ=255)
                                     tGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACACCCTCCATCAAGCTGa                                                               >  2:4334410/1‑100 (MQ=255)
                                     tGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTACTGCGTATACCACGCTTTCCATCGTGGTTTTTAACTACATCCTCCATCAAGc                                                                  >  1:2683941/1‑97 (MQ=255)
                                      ggAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAg                                                              <  2:3220893/100‑1 (MQ=255)
                                      ggAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAg                                                              <  2:1233009/100‑1 (MQ=255)
                                       gAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGc                                                             <  2:2028709/100‑1 (MQ=255)
                                       gAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGATGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCAACACGCTGAg                                                              <  1:3693415/99‑1 (MQ=255)
                                        aaaCTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCa                                                            <  1:80495/100‑1 (MQ=255)
                                           cTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCt                                                         >  2:2473860/1‑100 (MQ=255)
                                             ttCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTta                                                       <  1:506047/100‑1 (MQ=255)
                                             ttCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTta                                                       <  2:2549405/100‑1 (MQ=255)
                                              tCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtag                                                      <  1:1944776/100‑1 (MQ=255)
                                                   cGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggtagg                                                 >  2:867491/1‑100 (MQ=255)
                                                    gCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggtagg                                                 <  1:3122524/99‑1 (MQ=255)
                                                    gCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAggg                                                <  2:2769974/100‑1 (MQ=255)
                                                     ccAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGt                                               <  2:1304415/100‑1 (MQ=255)
                                                      cAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGta                                              <  2:1735543/100‑1 (MQ=255)
                                                         tttGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAg                                           <  2:3363556/100‑1 (MQ=255)
                                                          ttGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAg                                           <  1:1157759/99‑1 (MQ=255)
                                                          ttGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGc                                          <  1:2014034/100‑1 (MQ=255)
                                                           tGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGcc                                         <  2:69976/100‑1 (MQ=255)
                                                            gACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCg                                        <  2:920397/100‑1 (MQ=255)
                                                              cATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGaa                                      >  2:3521403/1‑100 (MQ=255)
                                                              cATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGa                                       >  2:3784918/1‑99 (MQ=255)
                                                                tgtgtgTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAgg                                    <  2:2316376/100‑1 (MQ=255)
                                                                  tgtgtAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTa                                  <  2:3386308/100‑1 (MQ=255)
                                                                   gtgtAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTAt                                 >  2:2670749/1‑100 (MQ=255)
                                                                   gtgtAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCAGCAGGCTGAGCACCTTAGGTAGGGTATAGCCGAAgg                                    <  1:4133378/97‑1 (MQ=255)
                                                                    tgtAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTAtt                                <  2:3925612/100‑1 (MQ=255)
                                                                      tAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTgg                              <  1:94478/100‑1 (MQ=255)
                                                                       aaTCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGt                             <  1:110857/100‑1 (MQ=255)
                                                                       aaTCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGt                             >  1:1751066/1‑100 (MQ=255)
                                                                       aaTCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGt                             <  1:3181800/100‑1 (MQ=255)
                                                                          cGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTAt                          >  2:2674306/1‑100 (MQ=255)
                                                                           gACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATg                         >  1:1050742/1‑100 (MQ=255)
                                                                            aCTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGa                        <  2:3141979/100‑1 (MQ=255)
                                                                             cTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCGCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGa                        <  1:1544739/99‑1 (MQ=255)
                                                                              tttGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGc                      <  2:431852/100‑1 (MQ=255)
                                                                                ctctgcGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCtt                    <  2:1644575/98‑1 (MQ=255)
                                                                                 gctgcGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTc                   <  2:771737/100‑1 (MQ=255)
                                                                                 gctgcGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTc                   >  2:784400/1‑100 (MQ=255)
                                                                                 gcagcGTATACCACGTTTTACAGCGTGGTGTTTCACTACTGCCACCCTCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGGTATGAGCTTc                   <  1:1889056/97‑1 (MQ=255)
                                                                                  ctgcGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCa                  >  2:1922871/1‑100 (MQ=255)
                                                                                  ctgcGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCa                  >  1:935576/1‑100 (MQ=255)
                                                                                    ttaaacaCCACGCTGTAAAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCaaa                >  2:679545/7‑100 (MQ=39)
                                                                                    gcGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCaaa                >  2:3048360/1‑100 (MQ=255)
                                                                                     cGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAAt               <  1:3910624/100‑1 (MQ=255)
                                                                                      gTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATc              <  1:4288957/100‑1 (MQ=255)
                                                                                       aacaCCACGCTGTAAAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTCGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATc              >  2:3916751/4‑99 (MQ=255)
                                                                                       aacaCCACGCTGTAAAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCa             >  1:2461968/4‑100 (MQ=255)
                                                                                        ataCCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAATCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCAt            <  1:2258374/100‑1 (MQ=255)
                                                                                        acaCCACGCTGTAAAGCGTGGTGTTTAACTACATCCCCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCCaataat            >  1:816901/3‑100 (MQ=255)
                                                                                         taCCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCAtt           >  2:3003158/1‑100 (MQ=255)
                                                                                         caCCACGCTGTAAAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGGATTGGTTATGAGCTTCAAATCa             >  1:998589/2‑98 (MQ=255)
                                                                                          aCCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCAttg          <  1:308276/100‑1 (MQ=255)
                                                                                          aCCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCAtt           >  1:1684907/1‑99 (MQ=255)
                                                                                          aCCACGCTGTAAAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCAttg          >  2:2051535/1‑100 (MQ=255)
                                                                                          aCCACGCTGTAAAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCAttg          >  2:2264106/1‑100 (MQ=255)
                                                                                           ccACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGACGGTATTGGTTATGAGCTTCAAATCAttgt         >  1:3520275/1‑100 (MQ=255)
                                                                                           ccACGCTGTAAAGCGTGGTGTTTAACTACATCCTCCATCACGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCAttgt         >  1:2156792/1‑100 (MQ=255)
                                                                                           ccACGCTGTAAAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCAttgt         >  2:2345571/1‑100 (MQ=255)
                                                                                               gCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTGAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAg     <  1:746540/100‑1 (MQ=255)
                                                                                                  ttACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGc  <  2:24326/100‑1 (MQ=255)
                                                                                                  ttACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGc  <  2:2216257/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
GCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGC  >  NC_000913/2761522‑2761719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: