BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,810,759	G→A	100%	intergenic (+80/‑11)	ygaH → / → mprA	putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,810,759	0	G	A	100.0%	609.7 / ‑6.7	182	intergenic (+80/‑11)	ygaH/mprA	putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand): ref base G (1/0); major base A (82/97); minor base C (2/0); total (85/97)

Only 100 of 183 total aligned reads displayed.

GTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGAC  >  NC_000913/2810662‑2810858

gTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATc                                                                                                   >  2:1167692/1‑100 (MQ=255)

gTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCCGAAATTAATGAGATc                                                                                                   <  1:1449223/100‑1 (MQ=255)

 tGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCa                                                                                                  <  1:4088755/100‑1 (MQ=255)

  gATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCAt                                                                                                 >  2:2620070/1‑100 (MQ=255)

  gATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCAt                                                                                                 >  2:2596805/1‑100 (MQ=255)

     ggCAATTATATAACCCACAAGAATCATTTTTATAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATAcc                                                                                              <  1:2257887/100‑1 (MQ=255)

       cGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCa                                                                                            <  1:3108093/100‑1 (MQ=255)

          ttATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAAt                                                                                         <  2:3996395/100‑1 (MQ=255)

            cttccgatctcaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATgg                                                                                       <  2:2436575/90‑1 (MQ=255)

            atataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATgg                                                                                       >  1:2598971/1‑100 (MQ=255)

             tataACCCACAAGAATCATTTTTCTAAAAGAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATg                                                                                        <  1:2392597/98‑1 (MQ=255)

               tcaCCCACAAGAACCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATa                                                                                    >  2:2964913/3‑100 (MQ=255)

               taACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATa                                                                                    >  2:976302/1‑100 (MQ=255)

                aaCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAg                                                                                   >  2:2019323/1‑100 (MQ=255)

                 aCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGt                                                                                  >  1:4335214/1‑100 (MQ=255)

                  cccACAAGAATCATTTTTTTAAATTAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAg                                                                                   >  1:3780806/1‑98 (MQ=255)

                  cccACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGtt                                                                                 >  2:2338479/1‑100 (MQ=255)

                   ccACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGtt                                                                                 <  2:1935159/99‑1 (MQ=255)

                   ccACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTc                                                                                >  1:4323696/1‑100 (MQ=255)

                   ccACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTc                                                                                <  1:3757119/100‑1 (MQ=255)

                    cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCg                                                                               <  2:1792185/100‑1 (MQ=255)

                     acaacaATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTAATATATTGGCTGAAATTAATGAGATCATACCCGAATGGATAGTTCGt                                                                              <  1:1787615/100‑1 (MQ=255)

                      caAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGtt                                                                             >  1:104737/1‑100 (MQ=255)

                      caAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGtt                                                                             >  1:1171808/1‑100 (MQ=255)

                        aGAATCATTTTTCTAAAACAATGCATTTACTTTATTTCTCACTGTCGGTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTa                                                                           <  1:2974379/100‑1 (MQ=255)

                        aGAATCATTTTTCTAAAACAATACATGTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGtt                                                                             <  1:1614699/98‑1 (MQ=255)

                          aaTCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACg                                                                         <  2:1327572/100‑1 (MQ=255)

                            tCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGcc                                                                       <  2:1912976/100‑1 (MQ=255)

                             cATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGccc                                                                      >  1:1596431/1‑100 (MQ=255)

                              aTTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGccc                                                                      >  1:4003176/1‑99 (MQ=255)

                              aTTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCa                                                                     >  2:519179/1‑100 (MQ=255)

                                ttttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCAtt                                                                   <  2:3655974/100‑1 (MQ=255)

                                 tttCTAAAACAATACATTTACTTTATTTTTCACTGTCGTTACTATATCGGCTTAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTg                                                                  <  1:1077685/100‑1 (MQ=255)

                                 tttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTg                                                                  >  1:4355220/1‑100 (MQ=255)

                                 tttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTg                                                                  <  2:327064/100‑1 (MQ=255)

                                 tttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTg                                                                  <  1:3435452/100‑1 (MQ=255)

                                 tttCTAAAACAATACATCTAATTTATTTGTCATTGTCTTTACTATATCGGCTGAAATTAATGAACTGATACCAAAATGGATAGTTCGTTTACGCCCAtt                                                                   >  1:276421/1‑99 (MQ=255)

                                   tCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGaa                                                                <  1:2940720/100‑1 (MQ=255)

                                    cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAAc                                                               <  2:4142372/100‑1 (MQ=255)

                                    cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAAc                                                               <  1:664138/100‑1 (MQ=255)

                                      aaaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaa                                                             >  2:310214/1‑100 (MQ=255)

                                        aaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAAt                                                           <  1:3344084/100‑1 (MQ=255)

                                        aaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAAt                                                           <  1:2469051/100‑1 (MQ=255)

                                         aCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATg                                                          <  2:3701909/100‑1 (MQ=255)

                                            aTACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTa                                                       <  1:4173377/100‑1 (MQ=255)

                                               cATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaaaa                                                    >  1:3822755/1‑100 (MQ=255)

                                                aTTTACTTTATTTGTCACTGTCGTTTCTATATAGGCTGAAATTAATGATATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAAt                                                   <  2:1281354/100‑1 (MQ=255)

                                                aTTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAAt                                                   <  1:3404222/100‑1 (MQ=255)

                                                 tttACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAAtt                                                  >  2:3818464/1‑100 (MQ=255)

                                                  ttACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAAttt                                                 >  1:3459867/1‑100 (MQ=255)

                                                  ttACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCACATTGAACAAATGCTAAAAttt                                                 >  1:805656/1‑100 (MQ=255)

                                                   tACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTAGTTTACGCCCATTGAACAAATGCTAAAATTTc                                                <  2:881053/100‑1 (MQ=255)

                                                    aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                               >  2:352287/1‑100 (MQ=255)

                                                    aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                               >  2:4244620/1‑100 (MQ=255)

                                                     cTTTATTTGTCACTGTCGTTACTATATCGGTTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                              <  2:2094351/100‑1 (MQ=255)

                                                     cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                              <  2:3354020/100‑1 (MQ=255)

                                                     cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                              >  2:2721472/1‑100 (MQ=255)

                                                      tttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgcg                                             <  2:4011722/100‑1 (MQ=255)

                                                       ttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCgcgc                                            >  2:3345153/1‑100 (MQ=255)

                                                       ttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCgcgc                                            <  2:2681910/100‑1 (MQ=255)

                                                       ttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCgcgc                                            <  2:673132/100‑1 (MQ=255)

                                                        tATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGcc                                           >  1:2075692/1‑100 (MQ=255)

                                                          tttGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAg                                         <  1:1767608/100‑1 (MQ=255)

                                                           ttGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgc                                        <  2:3092050/100‑1 (MQ=255)

                                                             gTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgccg                                      <  1:2209056/100‑1 (MQ=255)

                                                             gTCACTGTCGTTACTATATCGACTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgccg                                      <  1:3195011/100‑1 (MQ=255)

                                                              tCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgccgc                                     >  1:600194/1‑100 (MQ=255)

                                                                   gTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGa                                >  1:4311420/1‑100 (MQ=255)

                                                                   gTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCAACGa                                >  2:287863/1‑100 (MQ=255)

                                                                    tCGTTACTACATCGGCTGAAATTAATGAGAACAAACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGaa                               >  1:2893590/1‑100 (MQ=255)

                                                                     cGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAg                              >  1:3227678/1‑100 (MQ=255)

                                                                         aCTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAttt                          >  1:2711205/1‑100 (MQ=255)

                                                                            atatCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                       >  2:652676/1‑100 (MQ=255)

                                                                            atatCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                       <  1:1936656/100‑1 (MQ=255)

                                                                            atatCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                       <  1:2183192/100‑1 (MQ=255)

                                                                            atatCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                       <  2:1862076/100‑1 (MQ=255)

                                                                            atatCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                       >  1:1439756/1‑100 (MQ=255)

                                                                              atCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCtt                     >  1:2976130/1‑100 (MQ=255)

                                                                              atCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCCCCACGAAGATTTTCCtt                     >  1:1316525/1‑100 (MQ=255)

                                                                                cGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTa                    <  2:3573126/99‑1 (MQ=255)

                                                                                 ggCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATc                  <  1:3051411/100‑1 (MQ=255)

                                                                                 ggCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATc                  >  2:4097437/1‑100 (MQ=255)

                                                                                  gCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCa                 >  1:1004951/1‑100 (MQ=255)

                                                                                  gCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCa                 <  1:2282051/100‑1 (MQ=255)

                                                                                    tGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAgg               <  2:318550/100‑1 (MQ=255)

                                                                                    tGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAgg               <  1:2833690/100‑1 (MQ=255)

                                                                                     gAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGga              <  2:183433/100‑1 (MQ=255)

                                                                                     gAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGga              <  2:732425/100‑1 (MQ=255)

                                                                                      aaaTTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGgag             <  1:274500/100‑1 (MQ=255)

                                                                                      aaaTTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGgag             <  2:1431102/100‑1 (MQ=255)

                                                                                       agttAATGCGATCATACCCAATTGGATAGTTCGTTTACGCCCGTTGAAGATATGCTAAGCTTTCGCGCCAGCCGCGACGAAGATTTGTCTTATCAggtg             <  1:108132/97‑3 (MQ=255)

                                                                                       aaTTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGgaga            <  2:600196/100‑1 (MQ=255)

                                                                                         ttAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATc          >  1:1671874/1‑100 (MQ=255)

                                                                                           aaTGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTTGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCt        <  2:4011557/100‑1 (MQ=255)

                                                                                           aaTGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCt        >  2:1048485/1‑100 (MQ=255)

                                                                                            aTGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCAAGCCGCCACGAAGATTTTCCTTATCAGgagaac          >  1:734080/1‑95 (MQ=255)

                                                                                             tGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTc      >  1:425517/1‑100 (MQ=255)

                                                                                             tGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTc      <  1:976298/100‑1 (MQ=255)

                                                                                             tGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTc      >  1:3687414/1‑100 (MQ=255)

                                                                                                 gTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCAGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGAc  <  1:2338468/100‑1 (MQ=255)

GTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGAC  >  NC_000913/2810662‑2810858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`