Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,810,873 A→G 15.8% H35R (CAC→CGC) ‡ mprA → transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,810,8730AG15.8% 431.3 / 73.2 191H35R (CAC→CGC) mprAtranscriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand):  ref base A (79/80);  new base G (9/21);  total (90/101)
Fisher's exact test for biased strand distribution p-value = 7.13e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.55e-02

Only 100 of 222 total aligned reads displayed.

ATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGA  >  NC_000913/2810774‑2810972
                                                                                                   |                                                                                                   
aTAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTtgcatgca                                                                                                     <  1:966170/100‑1 (MQ=255)
aTAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTtgcatgca                                                                                                     <  2:2711220/100‑1 (MQ=255)
  aGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGcatgcaca                                                                                                   <  1:934342/100‑1 (MQ=255)
  aGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGcatgcaca                                                                                                   >  2:305063/1‑100 (MQ=255)
      cGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGcatgcacatgca                                                                                               >  2:2581535/1‑100 (MQ=255)
       gTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCaa                                                                                              >  1:2273229/1‑100 (MQ=255)
       gTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCaaa                                                                                             >  2:2308529/1‑100 (MQ=255)
         ttACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCaaan                                                                                            <  2:4033293/100‑2 (MQ=255)
         ttACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaag                                                                                            <  1:2991096/100‑1 (MQ=255)
          tACGCCCATTGAACAAATGCTAAAATTTCGTGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaaga                                                                                           <  2:3184937/100‑2 (MQ=255)
          tACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGTCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaaga                                                                                           <  1:1728516/100‑2 (MQ=255)
          tACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaaga                                                                                           <  2:650389/100‑2 (MQ=255)
           aCGCCCATTGAACAAATGCTAAGATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagac                                                                                          <  1:2417874/100‑3 (MQ=255)
           aCGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagac                                                                                          <  1:1048481/100‑3 (MQ=255)
           aCGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagac                                                                                          <  1:3994932/100‑3 (MQ=255)
           aCGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagac                                                                                          <  2:3477727/100‑3 (MQ=255)
           aCGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagac                                                                                          <  1:1134171/100‑3 (MQ=255)
           aCGCCCATTGAACAAATGCTAAAATTGCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagac                                                                                          <  1:742090/100‑3 (MQ=255)
            cGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaagcaa                                                                                         >  2:1966902/1‑100 (MQ=255)
             gCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagacga                                                                                        <  2:687639/100‑5 (MQ=255)
             gCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaagcaag                                                                                        <  2:104737/100‑1 (MQ=255)
              cccATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagacgag                                                                                       <  2:2432909/100‑6 (MQ=255)
              cccATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagacgag                                                                                       <  1:310213/100‑6 (MQ=255)
              cccATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaagcaagc                                                                                       <  2:2871196/100‑1 (MQ=255)
                 gttGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCATCTTTGCATGCACATGCAAAGCAAgctgc                                                                                    >  1:4370673/2‑100 (MQ=255)
                    gAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTgg                                                                                 >  2:3303359/1‑100 (MQ=255)
                     aaCAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGga                                                                                <  1:2620057/100‑1 (MQ=255)
                      aCAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGgag                                                                               >  2:434312/1‑100 (MQ=255)
                         aaTGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAAc                                                                            >  2:2929887/1‑100 (MQ=255)
                         aaTGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAAc                                                                            <  1:3218100/100‑1 (MQ=255)
                           tGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCg                                                                          >  2:1446815/1‑100 (MQ=255)
                           tGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCg                                                                          <  1:2447083/100‑1 (MQ=255)
                             cTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCa                                                                        <  1:1150612/100‑1 (MQ=255)
                             cTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCa                                                                        >  2:3407143/1‑100 (MQ=255)
                               aaaaTTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAAt                                                                      >  1:1897285/1‑100 (MQ=255)
                                 aaTTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATaa                                                                    >  2:241047/1‑100 (MQ=255)
                                 aaTTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATaa                                                                    <  2:894445/100‑1 (MQ=255)
                                 aaTTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATaa                                                                    >  1:1872011/1‑100 (MQ=255)
                                  aTTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGGACATGCAAAGCAAGCTGCTGGAGAACCGCAATaaa                                                                   >  1:2115427/1‑100 (MQ=255)
                                   tttCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGCGATCCTTCTGACTCGTCTTTTCATGCCCATGCAAAGCAAGCTGCTGGAGAACCGCAATaaaa                                                                  >  1:721743/1‑100 (MQ=255)
                                    ttCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAAt                                                                 <  2:2244297/100‑1 (MQ=255)
                                    ttCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAAt                                                                 >  2:2833559/1‑100 (MQ=255)
                                       gcgcCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCt                                                              <  2:2349979/100‑1 (MQ=255)
                                        cgcCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTg                                                             <  2:1310857/100‑1 (MQ=255)
                                           cAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAg                                                          >  2:3836653/1‑100 (MQ=255)
                                           cAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAg                                                          <  2:276422/100‑1 (MQ=255)
                                                    cGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAgggg                                                 >  1:1343165/1‑100 (MQ=255)
                                                    cGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAgggg                                                 >  2:2354823/1‑100 (MQ=255)
                                                    cGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAgggg                                                 <  2:25222/100‑1 (MQ=255)
                                                    cGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAgggg                                                 >  1:1785374/1‑100 (MQ=255)
                                                    cGAAGATTTTCCTTATCAGGAGATCATTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAgggg                                                 >  1:1089557/1‑100 (MQ=255)
                                                      aaGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGa                                                <  2:1542621/99‑1 (MQ=255)
                                                      aaGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGAt                                               <  2:546835/100‑1 (MQ=255)
                                                       aGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGAtt                                              >  1:152675/1‑100 (MQ=255)
                                                          ttttCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAAc                                           <  1:3903725/100‑1 (MQ=255)
                                                           tttCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACg                                          >  1:102561/1‑100 (MQ=255)
                                                            ttCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTTGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACga                                         >  2:2444246/1‑100 (MQ=255)
                                                                ttATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACg                                     <  1:167466/100‑1 (MQ=255)
                                                                ttATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACg                                     <  1:1589508/100‑1 (MQ=255)
                                                                ttATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACg                                     <  1:3454520/100‑1 (MQ=255)
                                                                 tATCAGGAGATCCTTCTGACTCGTCGTTGCATGCCCATGCAAAGCACGCTGCTGGAGAACTGCAATCAAATGCTGAAGGCTCAGGGGTCTAACgatcgat                                    >  1:368396/1‑95 (MQ=255)
                                                                  aTCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACg                                     <  2:3227695/98‑1 (MQ=255)
                                                                   tCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCGAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGAGTAACGCGCCgtt                                   >  1:2498672/1‑99 (MQ=255)
                                                                   tCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttg                                  >  2:404198/1‑100 (MQ=255)
                                                                    cAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgt                                 <  1:1819045/100‑1 (MQ=255)
                                                                    cAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgt                                 <  2:1074071/100‑1 (MQ=255)
                                                                     aGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgtt                                >  1:1410893/1‑100 (MQ=255)
                                                                      ggAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGttt                               <  1:1851620/100‑1 (MQ=255)
                                                                         gaTCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATg                            <  1:3734465/100‑1 (MQ=255)
                                                                          aTCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATgg                           <  2:2820080/100‑1 (MQ=255)
                                                                           tCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGc                          >  2:2905023/1‑100 (MQ=255)
                                                                           tCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGc                          >  1:1273243/1‑100 (MQ=255)
                                                                           tCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGc                          >  2:427512/1‑100 (MQ=255)
                                                                            ccTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCg                         <  1:3723598/100‑1 (MQ=255)
                                                                             cTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAACTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGt                        >  2:2476381/1‑100 (MQ=255)
                                                                              ttCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGtt                       <  2:1783100/100‑1 (MQ=255)
                                                                               tCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTg                      <  2:3682984/100‑1 (MQ=255)
                                                                                cTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGa                     <  2:4119809/100‑1 (MQ=255)
                                                                                agcagcttgcTTTGCATGTGCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGa                     >  1:305455/10‑100 (MQ=38)
                                                                                 gcagcttgcTTTGCATGTGCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGGTGAAGGCCCAGGGGATTAACGAGCCGTTGTTTATGGCGTTGAt                    >  1:2812426/9‑100 (MQ=37)
                                                                                  gACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGAtt                   >  1:4104968/1‑100 (MQ=255)
                                                                                   aCTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTa                  <  2:591859/100‑1 (MQ=255)
                                                                                    cTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTAc                 >  2:3735893/1‑100 (MQ=255)
                                                                                       tgcTTTGCATGTGCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTAGGCt              >  1:4009217/3‑100 (MQ=255)
                                                                                       gTCTTTGCATGCACATGCAAAGCAAGCTGGTGGAGAACCGCAATAAAATGCTGAAGGCGCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCt              <  1:2189478/100‑1 (MQ=255)
                                                                                       gTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCACGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCt              <  1:2707999/100‑1 (MQ=255)
                                                                                        gcTTTGCATGTGCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTg             >  2:3558397/2‑100 (MQ=255)
                                                                                          tttGTATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAATTGCTTAAGGATCAGGGGATTAACCAGGCGTTGTTTATGGCGTTGATTACg                <  1:589425/95‑1 (MQ=255)
                                                                                           ttGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTggtg          <  2:2967894/100‑3 (MQ=255)
                                                                                           ttGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAg          >  2:1427258/1‑100 (MQ=255)
                                                                                             gcatgtaCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtc        >  1:1218544/1‑100 (MQ=255)
                                                                                             gcatgcaCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtc        >  2:1490585/1‑100 (MQ=255)
                                                                                              catgcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtct       >  1:4273810/1‑100 (MQ=255)
                                                                                                tgcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCa     <  1:3542993/100‑1 (MQ=255)
                                                                                                tgcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCa     <  2:2191684/100‑1 (MQ=255)
                                                                                                tgcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCa     >  2:758365/1‑100 (MQ=255)
                                                                                                 gcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAg    <  2:3247415/100‑1 (MQ=255)
                                                                                                  cacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAgg   <  2:1649979/100‑1 (MQ=255)
                                                                                                  cacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAgg   >  2:627612/1‑100 (MQ=255)
                                                                                                  cacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAgg   >  1:1883762/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
ATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGA  >  NC_000913/2810774‑2810972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: