Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,976,320 T→G 16.5% intergenic (‑306/‑279) aas ← / → galR fused 2‑acylglycerophospho‑ethanolamine acyl transferase/acyl‑acyl carrier protein synthetase/galactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,976,3200TG16.5% 532.6 / 102.0 220intergenic (‑306/‑279)aas/galRfused 2‑acylglycerophospho‑ethanolamine acyl transferase/acyl‑acyl carrier protein synthetase/galactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base T (87/96);  new base G (22/14);  total (110/110)
Fisher's exact test for biased strand distribution p-value = 1.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.16e-01

Only 100 of 224 total aligned reads displayed.

GCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCAC  >  NC_000913/2976221‑2976419
                                                                                                   |                                                                                                   
gCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCAt                                                                                                     >  1:172436/1‑100 (MQ=255)
  cAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCt                                                                                                   >  2:375027/1‑100 (MQ=255)
     cGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTgcg                                                                                                <  2:3433504/100‑1 (MQ=255)
      gagaAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTgcgc                                                                                               >  2:820169/1‑100 (MQ=255)
       agaAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCa                                                                                              <  1:883473/100‑1 (MQ=255)
         aaaaCGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCAGATGCGCAgg                                                                                            <  1:2335335/100‑1 (MQ=255)
          aaaCGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCAGATGCGCAggt                                                                                           <  2:3252814/100‑2 (MQ=255)
          aaaCGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCAGATGCGCAggt                                                                                           <  1:831547/100‑2 (MQ=255)
           aaCGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCt                                                                                          >  2:1131995/1‑100 (MQ=255)
           aaCGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCt                                                                                          <  1:2034854/100‑1 (MQ=255)
            aCGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCAGATGCGCAggttt                                                                                         <  2:2348454/100‑4 (MQ=255)
            aCGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCAGATGCGCAggttt                                                                                         <  2:2658081/100‑4 (MQ=255)
             cggaaaGGAAGGAAGGGAAATGCAACGAGGGGTGTAAATTGTCGGTGACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTg                                                                                         <  1:655823/96‑1 (MQ=255)
             cGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCt                                                                                          >  2:1698679/1‑98 (MQ=255)
              gCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGgt                                                                                       >  2:1767737/1‑100 (MQ=255)
               cAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGgtg                                                                                      >  1:534736/1‑100 (MQ=255)
                aaaGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCAGATGCGCAggttttttt                                                                                     <  2:1081931/100‑8 (MQ=255)
                   ggAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAGCCTGCGCAGATGCGCAggttttttttgc                                                                                  <  2:4400982/100‑11 (MQ=37)
                   ggAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCAGATGCGCAggttttttttgc                                                                                  <  2:1911350/100‑11 (MQ=38)
                       tGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCAt                                                                              >  1:2849874/1‑100 (MQ=255)
                        gAAGGGAAATGCAACGAGGTTTGTAAATTGTCGGTTACGGGTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTGATTCAtg                                                                             <  1:624810/100‑1 (MQ=255)
                        gAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCAtg                                                                             <  2:4350805/100‑1 (MQ=255)
                          aGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCAtgtg                                                                           <  2:1813267/100‑1 (MQ=255)
                          aGGAAAATGCAACCAAGTAAGGAAATTGTCCGGAACTGTTAAAGATTAAAAAAAAAAAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCAtg                                                                             <  1:4127465/98‑1 (MQ=12)
                           gggAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGc                                                                          >  2:3027090/1‑100 (MQ=255)
                           gggAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCAACTGCCCAGGCTGGTGTAATTCAAGTGc                                                                          >  1:1827677/1‑100 (MQ=255)
                            ggAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCt                                                                         >  2:1522138/1‑100 (MQ=255)
                                 tGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAAc                                                                     >  2:3558920/1‑99 (MQ=255)
                                  gCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAAccc                                                                   <  1:3883467/100‑1 (MQ=255)
                                  gCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAAccc                                                                   >  1:3740138/1‑100 (MQ=255)
                                    aaCGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAAcc                                                                    <  1:552105/97‑1 (MQ=255)
                                    aaCGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGa                                                                 >  2:3335980/1‑100 (MQ=255)
                                        aGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGt                                                              <  1:1202399/99‑1 (MQ=255)
                                          gtgtgtAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGa                                                           <  1:993247/100‑1 (MQ=255)
                                           tgtgtAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGAc                                                          <  2:4242339/100‑1 (MQ=255)
                                              gtAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTc                                                       >  2:911782/1‑100 (MQ=255)
                                              gtAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTc                                                       >  2:3915806/1‑100 (MQ=255)
                                              gtAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTc                                                       <  2:557480/100‑1 (MQ=255)
                                               tAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCa                                                      >  1:36599/1‑100 (MQ=255)
                                               tAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCa                                                      >  2:3170896/1‑100 (MQ=255)
                                               tAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCa                                                      >  1:2606872/1‑100 (MQ=255)
                                               tAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCa                                                      <  2:4253004/100‑1 (MQ=255)
                                                 aaTTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCAcc                                                    <  2:2420019/100‑1 (MQ=255)
                                                 aaTTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCAcc                                                    >  2:503262/1‑100 (MQ=255)
                                                   ttGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTa                                                  <  2:2606885/100‑1 (MQ=255)
                                                     gTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATc                                                <  1:3335963/100‑1 (MQ=255)
                                                     gTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATc                                                <  2:1549348/100‑1 (MQ=255)
                                                     gTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATc                                                <  1:1767731/100‑1 (MQ=255)
                                                       cGGGTACTGTTACAGGTTGATGACCGGCAAAAAAAAACTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTTAACCCGAAGTTGACTTCACCTa                                                  >  1:1992549/1‑96 (MQ=255)
                                                         gTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATa                                            <  1:3513338/100‑1 (MQ=255)
                                                           tACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTTACTTCACCTATCAATAcc                                          <  1:1434951/100‑1 (MQ=255)
                                                           tACTGTTACAGATTGAAGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAGTTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATAcc                                          <  1:2403601/100‑1 (MQ=255)
                                                             cTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACctc                                        <  2:1025375/100‑1 (MQ=255)
                                                               gTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTg                                      <  1:1172660/100‑1 (MQ=255)
                                                               gTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTg                                      >  2:1592075/1‑100 (MQ=255)
                                                               gTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTg                                      <  1:3816088/100‑1 (MQ=255)
                                                               gTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTg                                      >  1:4283894/1‑100 (MQ=255)
                                                                 tACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTggg                                    >  1:1017383/1‑100 (MQ=255)
                                                                 tACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTggg                                    >  2:2254996/1‑100 (MQ=255)
                                                                  aCAGGTTGATGACTGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTggg                                    <  1:2180782/99‑1 (MQ=255)
                                                                  aCAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGa                                   <  1:503260/100‑1 (MQ=255)
                                                                       tagatgaGCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAAGTTATGTGCGCAACCCGAAGGTGACTTCACCTATCAATACCTCTGGGATca                                <  1:4214631/96‑1 (MQ=38)
                                                                         gatgaCCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATcaccac                            >  1:1894418/1‑100 (MQ=255)
                                                                          atgaCCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACt                           <  2:2204057/100‑1 (MQ=255)
                                                                          atgaCCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACt                           <  1:355206/100‑1 (MQ=255)
                                                                            naCCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACttt                         >  1:3304962/2‑100 (MQ=255)
                                                                              ccGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAg                       <  2:4318052/100‑1 (MQ=255)
                                                                              ccGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAg                       >  1:2489604/1‑100 (MQ=255)
                                                                              ccGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAg                       <  1:2549582/100‑1 (MQ=255)
                                                                              ccGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAg                       >  2:4012240/1‑100 (MQ=255)
                                                                              ccGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAg                       <  2:3705861/100‑1 (MQ=255)
                                                                              ccGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAg                       <  2:3296793/100‑1 (MQ=255)
                                                                              ccGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATACATGTGCTCAACCCGAACTTGAATTCAACTATCAATACCTCAGGGATcaccac                            >  1:1232331/1‑95 (MQ=38)
                                                                               cGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGc                      <  1:787533/100‑1 (MQ=255)
                                                                               cGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGc                      >  2:2857165/1‑100 (MQ=255)
                                                                                ggCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCa                     >  2:1570641/1‑100 (MQ=255)
                                                                                  cAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGc                      <  1:1551825/97‑1 (MQ=255)
                                                                                   tacaccagccTGCGCAGATGCGCAGGTTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAAcc                  >  2:2502892/9‑100 (MQ=38)
                                                                                    acaccagccTGCGCAGATGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCt                 >  1:3932082/8‑100 (MQ=255)
                                                                                     caccagccTGCGCAGATGCGCAGGTTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTg                >  2:4077615/7‑100 (MQ=38)
                                                                                     aaaaaaCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTg                >  2:3772865/1‑100 (MQ=255)
                                                                                      accagccTGCGCAGATGCGCAGGCTGGGGTAATGCATGTGCTCAACACGAAGTGTACGTCACCGATCAATACCGCTGGGAGCACCACTTTAGCAACCTGa               >  2:3084086/6‑100 (MQ=255)
                                                                                       ccagccTGCGCAGATGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGaa              >  2:765147/5‑100 (MQ=255)
                                                                                       ccagccTGCGCAGATGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGaa              >  2:4259625/5‑100 (MQ=255)
                                                                                       aaaaCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGaa              <  1:3108589/100‑1 (MQ=255)
                                                                                       aaaaCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGaa              >  2:463064/1‑100 (MQ=255)
                                                                                        aaaCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAg             <  1:3414402/100‑1 (MQ=255)
                                                                                         agccTGCGCAGATGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGCACTTCACTTCCCCTATCAAAACCTCTGGCATCACCACTATAGCAACCTGacg             >  1:2570583/3‑97 (MQ=255)
                                                                                         agccTGCGCAGATGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGaa              >  1:2942912/3‑98 (MQ=255)
                                                                                         agccTGCGCAGATGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGc            >  2:547605/3‑100 (MQ=255)
                                                                                         aaCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGc            <  2:3181675/100‑1 (MQ=255)
                                                                                         aaCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGc            <  2:606075/100‑1 (MQ=255)
                                                                                          gccTGCGCAGATGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGcc           >  1:20877/2‑100 (MQ=255)
                                                                                          gccTGCGCAGATGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGcc           >  2:3379631/2‑100 (MQ=255)
                                                                                          aCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGcc           <  2:351814/100‑1 (MQ=255)
                                                                                           ccTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCa          >  2:3058502/1‑100 (MQ=255)
                                                                                           ccTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCa          >  2:1397081/1‑100 (MQ=255)
                                                                                             tGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCaaa        >  1:305873/1‑100 (MQ=255)
                                                                                                 cATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGcc    >  2:1219431/1‑100 (MQ=255)
                                                                                                  aTCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGcca   <  2:2582093/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
GCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCAC  >  NC_000913/2976221‑2976419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: