Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,007,473 G→A 16.6% intergenic (+21/‑37) ygeW → / → ygeX putative carbamoyltransferase/2,3‑diaminopropionate ammonia lyase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,007,4730GA16.6% 498.1 / 75.2 209intergenic (+21/‑37)ygeW/ygeXputative carbamoyltransferase/2,3‑diaminopropionate ammonia lyase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base G (74/99);  new base A (12/23);  total (86/123)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 213 total aligned reads displayed.

ATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCA  >  NC_000913/3007375‑3007572
                                                                                                  |                                                                                                   
atCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGCAAGAAGCGCAAGAACGCGTGCAATAACCCCTCGGGCCGATCAGCTGGt                                                                                                    >  1:735127/1‑100 (MQ=255)
   cTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCgg                                                                                                 <  1:3022263/100‑1 (MQ=255)
   cTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCgg                                                                                                 <  1:3247075/100‑1 (MQ=255)
    tGTCCCGTAGATACGCCAAACCAGGTGCACTGCTCGGGCAACTGATGAAAGAAGCGCAACAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCgg                                                                                                 >  1:1708570/1‑99 (MQ=255)
     gTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGACCAGCTGATCGGcc                                                                                               <  2:936409/100‑1 (MQ=255)
     gTCCCGTAAATACGCCAAACCAGGTGCACAGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAGATAACCCCTCGGGCCGCTGAGCTGGTCGGc                                                                                                >  1:1168525/1‑99 (MQ=255)
      tCCCGTAAATACGCTAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGACCAGCTGATCGGccc                                                                                              <  2:27127/100‑1 (MQ=255)
      tCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGccc                                                                                              <  2:2761917/100‑1 (MQ=255)
      tCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGccc                                                                                              <  1:605433/100‑1 (MQ=255)
      tCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGccc                                                                                              <  2:1706857/100‑1 (MQ=255)
      tCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGACCAGCTGATCGGccc                                                                                              <  2:389327/100‑1 (MQ=255)
      tCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGACCAGCTGATCGGccc                                                                                              <  2:3521227/100‑1 (MQ=255)
       cccGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGcc                                                                                               <  1:3545238/98‑1 (MQ=255)
       cccGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCct                                                                                             >  1:2259150/1‑100 (MQ=255)
       cccGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGACCAGCTGATCGGccc                                                                                              <  1:18879/99‑1 (MQ=255)
       cccGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCGGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGACCAGCTGATCGGcccg                                                                                             <  2:2495613/100‑2 (MQ=255)
        ccGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCctc                                                                                            >  2:1403799/1‑100 (MQ=255)
         cGTAAATACGCCAAACCAGGTGCACTGCTCGAGCCACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGACCAGCTGATCGGcccgag                                                                                           <  2:199730/100‑4 (MQ=255)
         cGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGACCAGCTGATCGGcccgag                                                                                           <  2:153363/100‑4 (MQ=255)
          gTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTa                                                                                          <  2:1047073/100‑1 (MQ=255)
          gTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTa                                                                                          <  2:1671440/100‑1 (MQ=255)
          gTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGACCAGCTGATCGGcccgagg                                                                                          <  2:3366157/100‑5 (MQ=39)
           tAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGACCAGCTGATCGGcccgaggg                                                                                         <  2:3026655/100‑6 (MQ=39)
              aTACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTc                                                                                      <  1:104293/100‑1 (MQ=255)
               tACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTcc                                                                                     <  2:4254047/100‑1 (MQ=255)
                 cGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCag                                                                                   <  1:2594220/100‑1 (MQ=255)
                 cGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCag                                                                                   >  2:1828142/1‑100 (MQ=255)
                 cGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCag                                                                                   >  2:3796102/1‑100 (MQ=255)
                 cGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCag                                                                                   >  2:4005/1‑100 (MQ=255)
                 cGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCag                                                                                   >  2:2947523/1‑100 (MQ=255)
                 cGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCag                                                                                   >  2:712468/1‑100 (MQ=255)
                  gCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCaga                                                                                  <  2:497866/100‑1 (MQ=255)
                   ccAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCTAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCagag                                                                                 <  1:3093678/100‑1 (MQ=255)
                   ccAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCagag                                                                                 <  2:3655362/100‑1 (MQ=255)
                   ccAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCagag                                                                                 <  2:57142/100‑1 (MQ=255)
                   ccAAACCAGGTGCACTGCTCGAGCAACTGCAGAAAGAAGCGCAAGAACGAGTGAACTAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCa                                                                                    >  1:2870224/1‑97 (MQ=255)
                    cAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAgg                                                                                <  2:1222635/100‑1 (MQ=255)
                      aaCCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGc                                                                               <  2:3639267/99‑1 (MQ=255)
                       aCCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCa                                                                             <  1:1801055/100‑1 (MQ=255)
                        ccAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGc                                                                               <  1:4291550/97‑1 (MQ=255)
                           ggTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGCAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAag                                                                         >  2:1361789/1‑100 (MQ=255)
                              gCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggat                                                                      >  2:2657304/1‑100 (MQ=255)
                              gCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggat                                                                      >  1:1128873/1‑100 (MQ=255)
                              gCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggat                                                                      >  1:760388/1‑100 (MQ=255)
                               cACTGCTGGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCACAcggata                                                                     >  1:3678993/1‑100 (MQ=255)
                                aCTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggatag                                                                    <  2:652809/100‑1 (MQ=255)
                                  tGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggatagga                                                                  >  1:2401181/1‑100 (MQ=255)
                                  tGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggatagg                                                                   >  1:1805947/1‑99 (MQ=255)
                                   gCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggataggat                                                                 >  1:1285679/1‑100 (MQ=255)
                                   gCTCGAGCAACCGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggataggat                                                                 <  1:1320514/100‑1 (MQ=255)
                                     tCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGatat                                                               >  2:3048734/1‑100 (MQ=255)
                                       gAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGt                                                             >  1:395482/1‑100 (MQ=255)
                                          cAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCg                                                          <  2:1173160/100‑1 (MQ=255)
                                             ctgctgAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGttt                                                       <  1:3189299/100‑1 (MQ=255)
                                                  gAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCa                                                  >  2:1282124/1‑100 (MQ=255)
                                                  gAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCa                                                  >  1:1024893/1‑100 (MQ=255)
                                                  gAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCa                                                  >  2:1312885/1‑100 (MQ=255)
                                                  gAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCa                                                  >  2:1161263/1‑100 (MQ=255)
                                                   aaaGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCAt                                                 <  1:2206154/100‑1 (MQ=255)
                                                    aagaagCGCACGAACGCGTGAAATAACCCCTAGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCGtt                                                >  2:819584/1‑100 (MQ=255)
                                                     agaagCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTg                                               >  2:537115/1‑100 (MQ=255)
                                                        agCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAg                                            <  1:3786856/100‑1 (MQ=255)
                                                         gcgcAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGa                                           >  2:4121651/1‑100 (MQ=255)
                                                           gcAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGAtt                                         >  1:4077487/1‑100 (MQ=255)
                                                            cAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTg                                        <  2:95650/100‑1 (MQ=255)
                                                            cAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTg                                        >  1:3742373/1‑100 (MQ=255)
                                                             aaGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGa                                       <  2:2423224/100‑1 (MQ=255)
                                                             aaGAACGCGTGAAATAACCCCTCGGGCCGAACAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGa                                       <  1:1449249/100‑1 (MQ=255)
                                                                  cgcgTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCg                                  <  1:2733072/100‑1 (MQ=255)
                                                                    cgTGAAATAACCCCTCGGGTCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGcc                                <  1:1542408/100‑1 (MQ=255)
                                                                    cgTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGcc                                <  2:1128877/100‑1 (MQ=255)
                                                                      tGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGa                              <  2:451784/100‑1 (MQ=255)
                                                                       gAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGAt                             <  1:3184087/100‑1 (MQ=255)
                                                                         aaTAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATaa                           <  2:1417520/100‑1 (MQ=255)
                                                                         aaTAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATaa                           >  2:1760118/1‑100 (MQ=255)
                                                                          aTAACCCCTCGGGCCGATCAGCTGTTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAAc                          <  1:1579656/100‑1 (MQ=255)
                                                                             aCCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACaaa                       <  2:3845999/100‑1 (MQ=255)
                                                                             aCCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACaaa                       >  1:759766/1‑100 (MQ=255)
                                                                             aCCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACaa                        >  1:3611949/1‑99 (MQ=255)
                                                                              ccccTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAAt                      >  1:2607550/1‑100 (MQ=255)
                                                                                 cTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAAtttt                   <  1:1514012/100‑1 (MQ=255)
                                                                                 agagggCCGACCAGCTGATCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAAtttt                   >  2:2026108/4‑100 (MQ=255)
                                                                                  tCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAAttttt                  <  2:858817/100‑1 (MQ=255)
                                                                                   cGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTc                 <  1:4259553/100‑1 (MQ=255)
                                                                                    gggCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCa                <  1:1924932/100‑1 (MQ=255)
                                                                                     ggCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCaa               <  2:312489/100‑1 (MQ=255)
                                                                                     ggCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCaa               >  1:21487/1‑100 (MQ=255)
                                                                                      nccGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAAc              >  2:768279/2‑100 (MQ=255)
                                                                                      gCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAAc              >  1:3829562/1‑100 (MQ=255)
                                                                                       ccGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACg             <  1:4121622/100‑1 (MQ=255)
                                                                                          aTCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCg          <  2:2085024/100‑1 (MQ=255)
                                                                                          aTCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCg          <  2:1908712/100‑1 (MQ=255)
                                                                                          aTCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCg          <  2:1630566/100‑1 (MQ=255)
                                                                                            cAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTAAAGATTGATATCGGCGATAACAAATTTTTGAACg             >  1:1083805/1‑95 (MQ=255)
                                                                                             aGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGaaa       <  1:3100749/100‑1 (MQ=255)
                                                                                              gCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGaaa       >  1:2321689/1‑99 (MQ=255)
                                                                                              gCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAAc      >  2:37227/1‑100 (MQ=255)
                                                                                              gCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAAc      <  2:2347213/100‑1 (MQ=255)
                                                                                               cTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGTTGAAAAATTTGTTATCGGCGatatc     <  1:3441877/100‑5 (MQ=25)
                                                                                                 ggTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATc   <  2:4295993/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
ATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCA  >  NC_000913/3007375‑3007572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: