Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,181,298 A→T 17.5% H293L (CAT→CTT)  ygiC → ATP‑Grasp family ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,181,2980AT17.5% 430.6 / 80.3 183H293L (CAT→CTT) ygiCATP‑Grasp family ATPase
Reads supporting (aligned to +/- strand):  ref base A (82/68);  new base T (14/18);  total (97/86)
Fisher's exact test for biased strand distribution p-value = 3.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.00e-01

Only 100 of 199 total aligned reads displayed.

GGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGG  >  NC_000913/3181199‑3181397
                                                                                                   |                                                                                                   
ggAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGCATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGatca                                                                                                     >  1:2820155/1‑100 (MQ=255)
ggAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGatca                                                                                                     >  2:558992/1‑100 (MQ=255)
   agagCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACTCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATcc                                                                                                  <  1:637964/100‑1 (MQ=255)
   agagCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATcc                                                                                                  >  2:2627634/1‑100 (MQ=255)
   agagCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATcc                                                                                                  <  1:2033777/100‑1 (MQ=255)
      gCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCa                                                                                               >  2:3277957/1‑100 (MQ=255)
       cATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCaa                                                                                              >  2:507547/1‑100 (MQ=255)
        aTTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGATGATCATCTTCCGCaaa                                                                                             <  2:920357/100‑1 (MQ=255)
        aTTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCaaa                                                                                             >  2:996330/1‑100 (MQ=255)
        aTTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCaaa                                                                                             >  1:1400489/1‑100 (MQ=255)
         ttATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGATGATCATCTTCCGCaaaa                                                                                            <  2:1450886/100‑2 (MQ=39)
          tATCTCCAACACGGCACTTCTCCCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATg                                                                                           <  2:3506912/100‑1 (MQ=255)
          tATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGATGATCATCTTCCGCaaaat                                                                                           <  1:1260299/100‑3 (MQ=38)
          tATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATg                                                                                           <  1:1606144/100‑1 (MQ=255)
          tATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATg                                                                                           <  2:1592843/100‑1 (MQ=255)
           atttcCAACAAGGCACGTGTACAGCGATTGTGGGTGATGTTCTCGAAGCACCCGAACCTGCTGCTAGCTTATTTTGCGGAAGATGATCATCCGCAAATgg                                                                                          >  1:918701/4‑100 (MQ=255)
           aTCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGATGATCATCTTCCGCaaaata                                                                                          >  1:1564316/1‑97 (MQ=38)
           aTCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGATGATCATCTTCCGCaaaat                                                                                           <  2:1564321/99‑3 (MQ=39)
            tctcCAACAAGGCACTTCTACCCCTACTGTTGGAGATTTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGATGATCATCTTCCGCaaaat                                                                                           <  2:2937762/98‑3 (MQ=37)
             ctcCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGaa                                                                                        >  1:2369675/1‑100 (MQ=255)
             ctcCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGaa                                                                                        <  2:4355473/100‑1 (MQ=255)
             ctcCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGaa                                                                                        >  1:751669/1‑100 (MQ=255)
              tcCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGATGATCATCTTCCGCaaagtaagc                                                                                       <  1:3276002/100‑7 (MQ=38)
              tcCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGATGATCATCTTCCGCaaaataagc                                                                                       <  1:4383467/100‑7 (MQ=38)
              tcCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGaaa                                                                                       >  2:2281433/1‑100 (MQ=255)
              tcCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGaaa                                                                                       >  2:2357906/1‑100 (MQ=255)
              tcCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGaaa                                                                                       <  2:3994282/100‑1 (MQ=255)
               ccAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGATGATCATCTTCCGCaaaataagcg                                                                                      <  1:4263219/100‑8 (MQ=37)
               ccAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGaaaa                                                                                      >  1:3146539/1‑100 (MQ=255)
               ccAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGaaaa                                                                                      >  2:2776575/1‑100 (MQ=255)
                caacaaGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGATGATCATCTTCCGCaaaataagcgg                                                                                     <  2:1984636/100‑9 (MQ=37)
                  acaaGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAata                                                                                   <  1:3827690/100‑1 (MQ=255)
                    aaGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATAtg                                                                                 <  2:764409/100‑1 (MQ=255)
                     aGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATAtgt                                                                                <  2:2842831/100‑1 (MQ=255)
                      ggCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATAtgtg                                                                               <  1:737898/100‑1 (MQ=255)
                      ggCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCCGACGATCATCATCCGCAAATGCAAAAatat                                                                                  >  1:3525169/1‑97 (MQ=255)
                       gCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAGTATGTgg                                                                              <  2:2305250/100‑1 (MQ=255)
                       gCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTgg                                                                              <  2:4114755/100‑1 (MQ=255)
                        cACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGt                                                                             >  1:3957736/1‑100 (MQ=255)
                         aCTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCAACAACAAATAGAAAAATAtgtg                                                                               <  1:3332189/97‑1 (MQ=255)
                            tCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTaaa                                                                         >  1:2234253/1‑100 (MQ=255)
                             cTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAAc                                                                        <  1:935691/100‑1 (MQ=255)
                              tACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAAcc                                                                       <  1:566597/100‑1 (MQ=255)
                              tACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAAcc                                                                       >  1:425137/1‑100 (MQ=255)
                              tACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAAcc                                                                       <  2:2667875/100‑1 (MQ=255)
                               aCCGCTACTGTGGGAGAAGTTACCGAATCACCCGAACCTCCTGCCCGCTTATTTTGTGAAAGACGATTATCCGCAAATGGAAAAATATGGGGTTCAAcc                                                                       >  1:1998395/1‑99 (MQ=255)
                                     aCTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGAtcttc                                                                <  2:948356/100‑1 (MQ=255)
                                      cTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGAtcttct                                                               <  1:3078697/100‑1 (MQ=255)
                                        gtgGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTcc                                                             >  1:784158/1‑100 (MQ=255)
                                           ggAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTATGCGGAAGATGATCATCCGAAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGt                                                          >  1:2398188/1‑100 (MQ=255)
                                             agaTGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGa                                                        >  1:3402823/1‑100 (MQ=255)
                                             agaTGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGa                                                        <  1:2440218/100‑1 (MQ=255)
                                                tGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAgg                                                     >  1:3697336/1‑100 (MQ=255)
                                                   tCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGgcgc                                                  >  2:3781713/1‑100 (MQ=255)
                                                   tCCCGAATCACCCGAAAATGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGgcg                                                   >  1:1863047/1‑99 (MQ=255)
                                                       gAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAAc                                              <  1:4115105/100‑1 (MQ=255)
                                                        aaTCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACg                                             <  2:3607884/100‑1 (MQ=255)
                                                            aCCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACgtgt                                          >  1:1924597/1‑99 (MQ=255)
                                                             cccGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCGTCTCCCGTGAAGGCGCAAACGTGTCg                                        <  2:2679323/100‑1 (MQ=255)
                                                                   ccttttgcCTGCTTATTTTGCGTAAGTTGATTATCCGCAATTGGAAAAATATGTGGTTAACCCTATCTTCTCCCTTGATGGCGTAAACgtg                                           <  1:2862325/86‑1 (MQ=255)
                                                                    ctgctgCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTg                                 <  2:2398199/100‑1 (MQ=255)
                                                                     tgctgcCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTga                                >  1:1675311/1‑100 (MQ=255)
                                                                     tgctgcCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTg                                 <  2:3927033/99‑1 (MQ=255)
                                                                      gctgcCCGCTTATTTTGCGGAAGATGCTCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCGCCCGTGAAGGCGCAAACGTGTCGATCATTgag                               <  1:2418220/100‑1 (MQ=255)
                                                                      gctgcCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTgag                               <  2:1206371/100‑1 (MQ=255)
                                                                        tgcCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGaa                             >  1:1763333/1‑100 (MQ=255)
                                                                           ccGCTTATTTTGCGGAAGATGATCATCCGCAAATGTAAAAATAGGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACg                           <  2:3096310/99‑1 (MQ=255)
                                                                            cGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGc                         >  2:1218644/1‑100 (MQ=255)
                                                                            cGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGc                         >  2:1803708/1‑100 (MQ=255)
                                                                            cGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGc                         <  2:2883613/100‑1 (MQ=255)
                                                                            cGCTTATTTTACGGAAGATGATCATCCCCAAATGGCAAAATATGTGGTTAAACCGATCTGCTCCCGTGAAGGCACAATCGTGTCGATCATTGAGAAc                            <  1:3005833/97‑1 (MQ=255)
                                                                               tccatttGCGGATGATCATCTTCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCaaa                      >  2:3129084/5‑100 (MQ=38)
                                                                               tccatttGCGGATGATCATCTTCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCaaa                      >  2:1263547/5‑100 (MQ=38)
                                                                                ccatttGCGGATGATCATCTTCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCaaaa                     >  2:2183584/4‑100 (MQ=38)
                                                                                 catttGCGGATGATCATCTTCCGCAAATGGAAAAATATGTGGTTAAACCGCTCTTCTCCTGTGAACGCGCCAACGTGTCGACCATTGAGAACGGCaaaa                     >  1:1277968/3‑99 (MQ=37)
                                                                                 catttGCGGATGATCATCTTCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAAc                    >  2:1068791/3‑100 (MQ=39)
                                                                                 catttGCGGATGATCATCTTCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAAc                    >  1:824238/3‑100 (MQ=39)
                                                                                 aTTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAAc                    >  1:4149291/1‑100 (MQ=255)
                                                                                 aTTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAAc                    <  1:1329892/100‑1 (MQ=255)
                                                                                 aTTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAAc                    >  2:1690996/1‑100 (MQ=255)
                                                                                  atttGCGGATGATCATCTTCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAAcc                   >  2:2293952/2‑100 (MQ=39)
                                                                                   tttGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCa                  <  2:814176/100‑1 (MQ=255)
                                                                                    ttGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCAt                 <  2:852549/100‑1 (MQ=255)
                                                                                      gCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTg               <  1:1706779/100‑1 (MQ=255)
                                                                                      gCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTg               <  2:3314278/100‑1 (MQ=255)
                                                                                      gCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTg               <  1:3460782/100‑1 (MQ=255)
                                                                                       cGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGa              >  2:1761770/1‑100 (MQ=255)
                                                                                       cGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGa              >  1:2633309/1‑100 (MQ=255)
                                                                                         gAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAag            >  2:4232388/1‑100 (MQ=255)
                                                                                         gAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAag            >  2:742993/1‑100 (MQ=255)
                                                                                          aaGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAagc           <  2:4263758/100‑1 (MQ=255)
                                                                                             atgatCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAagcagc        <  1:3485382/100‑1 (MQ=255)
                                                                                             atgatCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAagcagc        <  1:4083461/100‑1 (MQ=255)
                                                                                              tgatCATCCGCAATTGGAAAAATATGTGGTTAAACCGAGCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCg       >  1:2714747/1‑100 (MQ=255)
                                                                                                atcatcCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGa     >  2:373966/1‑100 (MQ=255)
                                                                                                atcatcCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGa     >  1:2541331/1‑100 (MQ=255)
                                                                                                 tcatcCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGaa    <  2:2525726/100‑1 (MQ=255)
                                                                                                 tcatcCGCAAATGGAAAAATAGGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGAACATTGAGAACGGCAAAACCATTGAAGCAGCGGaa    <  1:682630/100‑1 (MQ=255)
                                                                                                  catcCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAg   >  1:1541069/1‑100 (MQ=255)
                                                                                                   atcCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAgg  >  1:209127/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
GGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGG  >  NC_000913/3181199‑3181397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑