Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,423,244 C→T 15.0% intergenic (+50/+179) yhdZ → / ← rrfF putative amino acid ABC transporter ATPase/5S ribosomal RNA of rrnD operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,423,2440CT15.0% 680.8 / 76.8 232intergenic (+50/+179)yhdZ/rrfFputative amino acid ABC transporter ATPase/5S ribosomal RNA of rrnD operon
Reads supporting (aligned to +/- strand):  ref base C (100/97);  new base T (33/2);  total (133/99)
Fisher's exact test for biased strand distribution p-value = 4.03e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.56e-01

Only 100 of 237 total aligned reads displayed.

ATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCC  >  NC_000913/3423145‑3423343
                                                                                                   |                                                                                                   
  ccTAAATCAGCGCGTACGAGGGCATTTTTAGCGCAGGGAATCCATGAATTGAATGTTAGTACGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCg                                                                                                    <  1:4312796/99‑1 (MQ=255)
   cTAAATCAGAGCGTACGAGGGCATTTTTATGGCTGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATAGCCTTTCGCt                                                                                                  >  1:2899113/1‑100 (MQ=255)
   cTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCt                                                                                                  >  2:3716300/1‑100 (MQ=255)
   cTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCt                                                                                                  <  2:3094830/100‑1 (MQ=255)
   cTAAATAAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCACTTAATTGAATGTTAGTTGGAAAAGAAAAAACGCCATCCTTTCGGATGGCCTTTCGCt                                                                                                  >  1:874710/1‑100 (MQ=255)
     aaaTCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCttg                                                                                                >  2:2702459/1‑100 (MQ=255)
     aaaTCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCttg                                                                                                >  2:1615093/1‑100 (MQ=255)
     aaaTCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCttg                                                                                                <  2:2079527/100‑1 (MQ=255)
     aaaTCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCttg                                                                                                <  1:3033214/100‑1 (MQ=255)
       aTCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTCAATGTTAGTTCGAAACGCAAAAAGGCCATCCTTTCGGATGGCCCTTCGCttgat                                                                                              >  2:3940586/1‑100 (MQ=255)
        tCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCttgatt                                                                                             >  1:2616369/1‑100 (MQ=255)
          agagCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCttgatttg                                                                                           <  1:2444327/100‑1 (MQ=255)
           gagCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCttgatttga                                                                                          <  1:4355229/100‑1 (MQ=255)
           gagCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCttgatttga                                                                                          <  2:3653173/100‑1 (MQ=255)
             gCGTACGAGGGCATTTTTCTCGCAGGTCATCCATTCCTTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATg                                                                                        <  1:2560740/100‑1 (MQ=38)
               gTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTc                                                                                      >  2:3508480/1‑100 (MQ=255)
                tACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCt                                                                                     >  2:3754357/1‑100 (MQ=255)
                tACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCt                                                                                     >  1:2885066/1‑100 (MQ=255)
                 aCGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTg                                                                                    <  1:1568430/100‑1 (MQ=255)
                  cGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTgg                                                                                   >  2:319979/1‑100 (MQ=255)
                   gAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGc                                                                                  <  1:4155459/100‑1 (MQ=255)
                   gAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGc                                                                                  <  2:758863/100‑1 (MQ=255)
                   gAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGc                                                                                  <  1:1835633/100‑1 (MQ=255)
                     gggCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAg                                                                                <  1:2292186/100‑1 (MQ=255)
                     gggCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAg                                                                                <  1:1144255/100‑1 (MQ=255)
                     gggCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTAATTTGATGTCTGGccg                                                                                >  1:1948251/1‑98 (MQ=255)
                        cATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGttt                                                                             <  2:137399/100‑1 (MQ=255)
                               aTCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATggcgg                                                                      >  2:1787337/1‑100 (MQ=255)
                               aTCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATggcgg                                                                      <  2:3882703/100‑1 (MQ=255)
                                 cGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATggcggg                                                                     >  2:1912874/1‑99 (MQ=255)
                                   cAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGt                                                                  <  2:2452875/100‑1 (MQ=255)
                                    aGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATggcgggcagg                                                                 <  2:4029812/100‑4 (MQ=255)
                                     ggTAATCCATTAATTGAATGTTAGTGCGAAAAGCAAAAAGGCCATCCTTTCGGTTGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTcc                                                                <  1:1089922/100‑1 (MQ=255)
                                       tAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATggcgggcaggacg                                                              <  1:1583506/100‑7 (MQ=255)
                                       tAATCCATTAATTGAATGTTAGTTCGAAAAGAAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGGGTCCTg                                                              >  1:4050416/1‑100 (MQ=255)
                                            ccgatcttGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGc                                                         <  2:3157964/94‑1 (MQ=255)
                                              ttAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGcc                                                        >  1:4247915/1‑99 (MQ=255)
                                              ttAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCa                                                       <  2:3621748/100‑1 (MQ=255)
                                               tAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCAc                                                      <  1:1395785/100‑1 (MQ=255)
                                                aaTTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCAcc                                                     >  2:1733105/1‑100 (MQ=255)
                                                aaTTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCAcc                                                     <  2:4238236/100‑1 (MQ=255)
                                                 aTTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCAccc                                                    >  1:163859/1‑100 (MQ=255)
                                                 aTTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCAccc                                                    >  1:2194437/1‑100 (MQ=255)
                                                 aTTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCAccc                                                    >  2:2567769/1‑100 (MQ=255)
                                                 aTTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCAccc                                                    >  1:1173234/1‑100 (MQ=255)
                                                      aTGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCgg                                               >  2:3363539/1‑100 (MQ=255)
                                                      aTGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCg                                                >  1:3125114/1‑99 (MQ=255)
                                                       tGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCggg                                              <  2:63627/100‑1 (MQ=255)
                                                         ttAGTTCGAAAAGCAAAAAGGCCATCCTTGCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGGTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGc                                             >  1:3221173/1‑99 (MQ=255)
                                                          tAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCg                                           <  2:1511969/100‑1 (MQ=255)
                                                           aGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGt                                          <  1:1390939/100‑1 (MQ=255)
                                                            gTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGtt                                         <  1:2006244/100‑1 (MQ=255)
                                                            gTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCGTCCCGCCACCCTCCGGGCCg                                           >  1:1331408/1‑98 (MQ=255)
                                                             ttCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTg                                        <  2:918680/100‑1 (MQ=255)
                                                              tCGAAAAGCAAAAAGGCCATCTTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGc                                       <  1:588556/100‑1 (MQ=255)
                                                               cGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCt                                      >  2:1360076/1‑100 (MQ=255)
                                                                 aaaaGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTc                                    <  2:924027/100‑1 (MQ=255)
                                                                 aaaaGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTc                                    >  1:4282288/1‑100 (MQ=255)
                                                                  aaaGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCg                                   <  2:545328/100‑1 (MQ=255)
                                                                  aaaGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCg                                   <  1:1703947/100‑1 (MQ=255)
                                                                   taagcagaaGGCCATCCTGACGGATGGCCTTCTGCTTAATTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGc                                  >  2:3402742/8‑100 (MQ=1)
                                                                     tgcagaaGGCCATCCTGACGGATGGCCTTCTGCTTAATTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCaa                                <  2:495581/95‑1 (MQ=1)
                                                                     gCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCaa                                >  2:3030351/1‑100 (MQ=255)
                                                                      gcagaaGGCCATCCTGACGGATGGCCTTCTGCTTAATTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCaaa                               >  1:495579/5‑99 (MQ=1)
                                                                      cAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAAc                               >  1:2958132/1‑100 (MQ=255)
                                                                      cAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAAc                               >  2:3421687/1‑100 (MQ=255)
                                                                      cAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGAGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAAc                               >  1:244510/1‑100 (MQ=255)
                                                                       cagaaGGCCATCCTGACGGATGGCCTTCTGCTTAATTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACg                              >  2:1000637/4‑100 (MQ=1)
                                                                       aaaaaGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACg                              >  2:1463203/1‑100 (MQ=255)
                                                                       aaaaaGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACg                              <  2:1816098/100‑1 (MQ=255)
                                                                       aaaaaGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACg                              <  2:1079742/100‑1 (MQ=255)
                                                                        gaaaGGCCATCCGAAAGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGt                             >  1:766897/2‑100 (MQ=11)
                                                                        aaaaGGCCAGCCTTTCGGATGGCCTTTCGCTTGATTTGAGGTCTGGCAGTTTATGGCGGGCGCCCTGCCCGCCACCCTCCGGGCCGTTGCGTc                                    >  1:3952143/1‑93 (MQ=18)
                                                                         gaaGGCCATCCTGACGGATGGCCTTCTGCTTAATTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGtt                            >  1:2677020/2‑100 (MQ=1)
                                                                         gaaGGCCATCCTGACGGATGGCCTTCTGCTTAATTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGtt                            >  1:825401/2‑100 (MQ=1)
                                                                         aaaGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGtt                            <  2:1160839/100‑1 (MQ=255)
                                                                         aaaGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGtt                            <  1:3435302/100‑1 (MQ=255)
                                                                           aGGCCATCCTGACGGATGGCCTTCTGCTTAATTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCCACGTTc                           >  1:3769189/1‑99 (MQ=1)
                                                                           aGGCCATCCTGACGGATGGCCTTCTGCTTAATTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCa                          >  2:2365993/1‑100 (MQ=1)
                                                                           aGGCCATCCTGACGGATGGCCTTCTGCTTAATTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCa                          >  2:3277075/1‑100 (MQ=1)
                                                                           aGGCCATCCTGACGGATGGCCTTCTGCTTAATTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCa                          >  2:4366175/1‑100 (MQ=1)
                                                                           aGGCCATCCTGACGGATGGCCTTCTGCTTAATTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCa                          >  2:839310/1‑100 (MQ=1)
                                                                            ggCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCaa                         <  1:3297295/100‑1 (MQ=255)
                                                                            ggCCATCCTGACGGATGGCCTTCTGCTTAATTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCaa                         >  2:3311472/1‑100 (MQ=1)
                                                                            ggCCATCCTGACGGATGGCCTTCTGCTTAATTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCCTCGCAACGTTCaa                         >  1:676614/1‑100 (MQ=1)
                                                                             gCCATCCTGACGGATGGCCTTCTGCTTAATTTGATGCCTGGCAGTTTATGGCGGGGGTCCTGCCCGCCACCCGCCGGGCCGTTGCTTCGCAACGTTCaaa                        >  1:362215/1‑100 (MQ=1)
                                                                             gCCATCCTGACGGATGGCCTTCTGCTTAATTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCaaa                        >  2:582647/1‑100 (MQ=1)
                                                                             gCCATCCTGACGGATGGCCTTCTGCTTAATTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCaaa                        >  2:2427610/1‑100 (MQ=1)
                                                                             gCCATCCTGACGGATGGCCTTCTGCTTAATTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCaaa                        >  2:1052762/1‑100 (MQ=1)
                                                                              ncATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAAt                       >  2:283932/2‑100 (MQ=255)
                                                                                aTCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATcc                     <  2:1325178/100‑1 (MQ=255)
                                                                                 tCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCCGTTTATGGCGTGCGTCCTGCCCGCGACCCTCCGGGCCGTTGCTTCGCCACGGTCAACTCCg                    >  1:1472940/1‑100 (MQ=17)
                                                                                 tCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCg                    <  1:319978/100‑1 (MQ=255)
                                                                                 tCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCg                    <  2:2885082/100‑1 (MQ=255)
                                                                                 tCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCg                    <  2:1947788/100‑1 (MQ=255)
                                                                                  ccTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGc                   <  2:2393288/100‑1 (MQ=255)
                                                                                            ggCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGa         <  1:2363197/100‑1 (MQ=255)
                                                                                             gCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCcggcggct        >  2:3993777/1‑98 (MQ=255)
                                                                                               cTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGAttt      >  1:358210/1‑100 (MQ=255)
                                                                                                  tCGCTTGTTTTGATGTGTGGCTGGTTATGGTGCGCGCCCTGCCCGCTACCCTCCGGGCCGTTGCTTCGCAGCGTTGAAATCCGCTCCCGGCGGATTTGTc   <  1:3603415/100‑1 (MQ=14)
                                                                                                   |                                                                                                   
ATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCC  >  NC_000913/3423145‑3423343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: