Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,705,985 T→C 40.0% intergenic (‑195/+113) dppB ← / ← dppA dipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,9850TC40.0% 346.7 / inf 332intergenic (‑195/+113)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base T (106/93);  new base C (126/6);  total (233/99)
Fisher's exact test for biased strand distribution p-value = 1.15e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.38e-04

Only 100 of 340 total aligned reads displayed.

GCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTT  >  NC_000913/3705887‑3706084
                                                                                                  |                                                                                                            
 ctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTAt                                                                                                            >  2:1117413/1‑100 (MQ=255)
 ctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTAt                                                                                                            >  2:2558601/1‑100 (MQ=255)
   gcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATcc                                                                                                          >  2:2894380/1‑100 (MQ=255)
      ggATGGCGCTGTACGCAGAGGCACAGCGCCACCGTGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGc                                                                                                       <  1:1011624/100‑1 (MQ=25)
      ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGc                                                                                                       <  2:522833/100‑1 (MQ=37)
       gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                                      <  1:62179/100‑1 (MQ=255)
            cgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTCATCCGGCCTACaa                                                                                                <  1:2714990/100‑1 (MQ=255)
               tGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAAt                                                                                             <  1:3445655/100‑1 (MQ=255)
               tGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAAt                                                                                             >  2:1259397/1‑100 (MQ=255)
               tGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTAGGCCGGATAAGGCGTTAACGCCGCATCCGGcataaacaaa                                                                                              <  2:4276910/100‑10 (MQ=21)
                gCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATc                                                                                            >  1:437807/1‑100 (MQ=255)
                 cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCg                                                                                           <  2:3665069/100‑1 (MQ=255)
                   cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                                         <  1:1842167/100‑1 (MQ=255)
                    gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                                        <  2:300097/100‑1 (MQ=255)
                        agGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAt                                                                                    >  1:2859126/1‑100 (MQ=255)
                        agGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAt                                                                                    <  1:2630240/100‑1 (MQ=255)
                                cgcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACa                                                                            <  2:1835161/100‑1 (MQ=255)
                                  ccACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAtt                                                                          >  1:1619533/1‑100 (MQ=255)
                                  ccACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAtt                                                                          >  2:922712/1‑100 (MQ=255)
                                   cACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTg                                                                         <  2:2727898/100‑1 (MQ=255)
                                       gggTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGa                                                                    >  1:1415152/1‑100 (MQ=255)
                                        ggTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGaa                                                                  >  1:1493618/1‑100 (MQ=255)
                                        ggTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGaa                                                                  >  2:1548510/1‑100 (MQ=255)
                                            taCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACCAAATCGTGCAAATTCAATACATTGCAGAAtt‑‑‑t                                                           >  2:2488582/1‑99 (MQ=255)
                                              cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑g                                                      <  2:2125560/100‑1 (MQ=255)
                                               aGATTGTGGACAAAGTGGGGGTTGTTTATGCCGGATGGGGGGTTAACGCCTTATCCGGCGTACAAAATCGTGCAAATTCAATACATTGGAGAAtt‑‑‑t                                                           >  1:4036073/1‑96 (MQ=14)
                                                gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTa                                                    >  1:4115884/1‑100 (MQ=255)
                                                gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTa                                                    <  1:3733186/100‑1 (MQ=255)
                                                  gcataaaCAAAGCGCACTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAgg                                                  >  2:975526/7‑100 (MQ=18)
                                                   tgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGc                                                 >  2:2514801/1‑100 (MQ=255)
                                                   tgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGc                                                 <  2:3929540/100‑1 (MQ=255)
                                                    gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGcc                                                <  2:1356045/100‑1 (MQ=255)
                                                    gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGcc                                                <  1:867488/100‑1 (MQ=255)
                                                      tgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTg                                              >  1:2002146/1‑100 (MQ=255)
                                                      tgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTg                                              <  2:4063820/100‑1 (MQ=255)
                                                       gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCACATTCACTACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCt                                               >  1:1551668/1‑98 (MQ=255)
                                                       gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGa                                             <  1:3223997/100‑1 (MQ=255)
                                                          aaaGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATaa                                          >  1:254817/1‑100 (MQ=255)
                                                            aGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGaaaa                                          >  1:1657215/1‑98 (MQ=255)
                                                              tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCgg                                      <  2:2403523/100‑1 (MQ=255)
                                                                gattttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAg                                    <  2:1141612/97‑1 (MQ=255)
                                                                cgcTTTGTTTACGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACACAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAg                                    <  1:2811269/100‑1 (MQ=255)
                                                                 gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATATGCGGAgc                                   <  2:2314723/100‑1 (MQ=255)
                                                                  cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgcg                                  >  1:532550/1‑100 (MQ=255)
                                                                  cTTTGTTTATGCCGAATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCTCGCAAATTCAATACATTGCCGAATT‑‑‑TTC‑‑GTAGGGCCGATAAGccg                                      >  1:3558499/1‑94 (MQ=17)
                                                                    ctttgtgggtcGGATAAGGCGTTCACGCCGCATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAAC‑‑‑GAT‑‑GTAGGCCTGATAAGCGTAGCGCa                                >  1:2015075/11‑100 (MQ=21)
                                                                     tttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTA‑‑‑TTG‑‑GTAGGCCTGATAAGCGCAGCGCAt                               >  2:449426/8‑100 (MQ=21)
                                                                     tGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCAt                               >  2:3691175/1‑100 (MQ=255)
                                                                     tGTTTATGCCGGAGGCTGCGTTAACGACTTACCCGGCATACAAAATCGTGCCAATTCACTACATTGCAGAATT‑‑‑TGC‑‑GTAGGCCTGATAAGCGGAgcg                                  <  1:3117850/97‑1 (MQ=17)
                                                                     cttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATAT‑‑‑GAC‑‑GTAGGCCTGATAAGCGTAGCGCATc                              >  2:4157011/8‑100 (MQ=18)
                                                                       tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCa                             <  1:754853/100‑1 (MQ=255)
                                                                       tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCa                             <  2:3407641/100‑1 (MQ=255)
                                                                        gtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAACATATTGCAATTCT‑‑‑CTT‑‑GTAGGCATGATAAGAGTAACGCATCAg                            >  1:2275138/5‑100 (MQ=12)
                                                                        gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCATGAGC‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCa                             >  2:3738793/5‑99 (MQ=17)
                                                                        gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAg                            >  2:1053409/5‑100 (MQ=17)
                                                                        gtaggCCGGATAAGGCGTTAACGCCGCATCCGGCATACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAg                            >  1:1720797/5‑100 (MQ=18)
                                                                         taggCCGGATAAGGCGTTCACGCCGCATCCGGCATACAAAACTTTGCAAATTCAATATATTGCAATC‑‑‑TCCGTGTAGGCCTGATAAGCGTAGCGCATCAgg                           >  2:3139000/4‑100 (MQ=18)
                                                                          aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGAAA‑‑‑TTC‑‑‑‑AGGCCTGATAAGCGTAGCGCATCAGGct                         >  2:1468175/3‑99 (MQ=25)
                                                                          aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                          >  1:1750264/3‑100 (MQ=14)
                                                                          aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGCAATC‑‑‑TCCGTGTAGGCCTGATAAGCGTAGCGCATCAGGc                          >  1:21078/3‑100 (MQ=18)
                                                                          aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATT‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                          >  2:2828031/3‑100 (MQ=17)
                                                                          aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAGT‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                         >  2:1998899/3‑100 (MQ=18)
                                                                          aggCCGGATAAGGCGTTCACGCCGCAACCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGAT‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                          >  2:2673004/3‑100 (MQ=17)
                                                                           tGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCa                         >  2:3325062/1‑100 (MQ=255)
                                                                           tGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCa                         >  1:1295317/1‑100 (MQ=255)
                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGACC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                         >  1:1321910/2‑100 (MQ=18)
                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTA‑‑‑TTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCa                         >  2:4007534/2‑100 (MQ=21)
                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTA‑‑‑TTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCa                         >  1:657558/2‑100 (MQ=21)
                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATC‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                         >  2:753226/2‑100 (MQ=21)
                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGC‑‑‑TGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                         >  1:3386122/2‑100 (MQ=17)
                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGCAATCTC‑‑‑CGT‑‑GTAGTCCTGATAAGCGTAGCGCATCAgg                           >  1:4079169/2‑98 (MQ=18)
                                                                            gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt                        >  1:4197984/1‑100 (MQ=255)
                                                                            gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAAC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag                        >  2:665223/1‑99 (MQ=18)
                                                                            gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAACAGTGCAAATTCAATATATTGCAATCTT‑‑‑CTG‑‑GTAGGCCTGATAAGCATCGCGCATCAGGCaa                        >  2:4168318/1‑99 (MQ=18)
                                                                            gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATC‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaa                        >  1:1085725/1‑99 (MQ=21)
                                                                            gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATAGCAGAGAT‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaa                        >  1:3081300/1‑99 (MQ=17)
                                                                             cgggataaggcgTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATT‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaat                       >  2:2978717/9‑98 (MQ=17)
                                                                             ccggataaggcgTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTT‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagt                       >  2:4344735/9‑98 (MQ=17)
                                                                             ccGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtt                       <  1:3007260/100‑1 (MQ=255)
                                                                             ccGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAAC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagt                       >  1:2759637/1‑98 (MQ=18)
                                                                              cggataaggcgTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTT‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgtt                      >  1:222894/8‑100 (MQ=17)
                                                                               ggataaggcgTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAAC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                     >  2:2073193/7‑100 (MQ=18)
                                                                               ggataaggcgTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTT‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                     >  2:132579/7‑100 (MQ=17)
                                                                               ggataaggcgTTTACGCCGCATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTT‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCaatt                      >  2:2369838/7‑97 (MQ=21)
                                                                               ggataaggcgTTCACGCCGCATCCTGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGAT‑‑‑GGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtttt                     >  1:3475694/7‑100 (MQ=14)
                                                                               ggataaggcgTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGACC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                     >  2:3068104/7‑100 (MQ=18)
                                                                               ggataaggcgTTCACGCCGCATCCGGCCTACGAAATCGTGCTAATTCAATATATTGTGGGCTC‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                     >  1:2413417/7‑100 (MQ=18)
                                                                               ggataaggcgTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTA‑‑‑TTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCaatct                     >  2:3560582/7‑96 (MQ=25)
                                                                               ggataaggcgTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAGG‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAttta                     >  2:1297258/7‑98 (MQ=18)
                                                                               ggATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtttt                     >  2:1594833/1‑100 (MQ=255)
                                                                               ggATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtttt                     <  2:1972368/100‑1 (MQ=255)
                                                                                  taaggcgTTCACGCCGCATCCTGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGAT‑‑‑GGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCATTtttgc                  >  2:4205153/4‑100 (MQ=14)
                                                                                  tGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgc                  <  2:1482304/100‑1 (MQ=255)
                                                                                     ggcgTTCACGCCGCATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCacgctcgcatg               >  1:326761/1‑90 (MQ=18)
                                                                                     ggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctt               >  2:224091/1‑100 (MQ=255)
                                                                                        gTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgc            <  1:3655507/100‑1 (MQ=255)
                                                                                         ttAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgcc           <  1:1043851/100‑1 (MQ=255)
                                                                                          tAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCa          >  2:1740001/1‑100 (MQ=255)
                                                                                                 ttATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCt   <  2:2639548/100‑1 (MQ=255)
                                                                                                  tATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCtt  <  1:4307040/100‑1 (MQ=255)
                                                                                                  |                                                                                                            
GCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTT  >  NC_000913/3705887‑3706084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: