Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,888,219 T→A 17.5% intergenic (+27/‑216) mnmE → / → tnaC tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase/tryptophanase leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,888,2190TA17.5% 496.5 / 95.2 207intergenic (+27/‑216)mnmE/tnaCtRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase/tryptophanase leader peptide
Reads supporting (aligned to +/- strand):  ref base T (87/83);  new base A (17/19);  total (104/103)
Fisher's exact test for biased strand distribution p-value = 7.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.83e-02

Only 100 of 215 total aligned reads displayed.

CGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAG  >  NC_000913/3888120‑3888318
                                                                                                   |                                                                                                    
 gAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTg                                                                                                     <  2:1416736/100‑1 (MQ=255)
    aTCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCa                                                                                                  <  2:3845484/100‑1 (MQ=255)
      cACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTAATGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGa                                                                                               >  1:2809909/1‑100 (MQ=255)
       aCCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGAt                                                                                              >  1:864381/1‑100 (MQ=255)
        ccGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATg                                                                                             >  2:1537824/1‑100 (MQ=255)
        ccGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATg                                                                                             >  1:2023402/1‑100 (MQ=255)
         cGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGc                                                                                            <  2:1429299/100‑1 (MQ=255)
         cGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGc                                                                                            <  2:4184629/100‑1 (MQ=255)
           gggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGATAGTCAGAATGCgg                                                                                          <  1:2723466/100‑1 (MQ=38)
            ggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCGgc                                                                                         <  1:1407154/100‑1 (MQ=38)
            ggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCGgc                                                                                         <  2:2956793/100‑1 (MQ=38)
             gAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgct                                                                                        <  2:841358/100‑1 (MQ=255)
              aaTTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCGgc                                                                                         <  2:1260513/98‑1 (MQ=38)
               aTTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCGTTCTGACTGTCAGATGCGgcttc                                                                                      >  1:2230436/1‑100 (MQ=255)
               aTTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttc                                                                                      <  1:2540359/100‑1 (MQ=255)
                tttACTTGAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcg                                                                                     <  1:1928508/100‑1 (MQ=255)
                tttACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcg                                                                                     <  1:3240287/100‑1 (MQ=255)
                 ttACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCGgcttcgt                                                                                    <  1:780023/100‑2 (MQ=37)
                    cTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcgcttc                                                                                 >  2:268572/1‑100 (MQ=255)
                    cTTCAGACGACCTGCTGGGGCGGATTTTCGCCCGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcgcttc                                                                                 >  1:1373913/1‑100 (MQ=255)
                      tCAGACGACCTGCTGGGGCGGATTTTGTCGAGCTGCTGTATTGGTAGGTAACTGCGCTTACGAAGCCGCATTGTGTCTGTTAGATCCGgcttcgc                                                                                    >  1:986155/1‑95 (MQ=255)
                      tCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCAt                                                                               >  1:3277149/1‑100 (MQ=255)
                        aGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTg                                                                             <  1:1542167/100‑1 (MQ=255)
                         gacgacCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTGCGAAGCCGCATCTGACAGTCAGAATGCGgcttcgtaagcgcgg                                                                            <  2:146558/100‑10 (MQ=18)
                         gacgacCTGCTGGGGCGGATTTTCTCCAGCTACTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGt                                                                            <  1:599357/100‑1 (MQ=255)
                           tctccTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTa                                                                          <  1:1660924/97‑1 (MQ=255)
                            gacCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTAc                                                                         <  1:1825727/100‑1 (MQ=255)
                            gacCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTAc                                                                         <  2:2174558/100‑1 (MQ=255)
                            gacCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTAc                                                                         <  1:517770/100‑1 (MQ=255)
                             acCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTAcc                                                                        >  2:2222716/1‑100 (MQ=255)
                             acCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTAcc                                                                        <  1:1068817/100‑1 (MQ=255)
                              ccTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTAcc                                                                        <  1:462786/99‑1 (MQ=255)
                              ccTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCa                                                                       >  2:3178121/1‑100 (MQ=255)
                               ctgctgGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCAc                                                                      <  2:3134837/100‑1 (MQ=255)
                                  ctgGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTcc                                                                   <  2:3975512/100‑1 (MQ=255)
                                    ggggCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTg                                                                 <  2:3258129/100‑1 (MQ=255)
                                      ggCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGtt                                                               >  2:584333/1‑100 (MQ=255)
                                       gCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTa                                                              >  1:293838/1‑100 (MQ=255)
                                        cGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTAt                                                             >  1:1478039/1‑100 (MQ=255)
                                        cGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTAt                                                             >  1:3378928/1‑100 (MQ=255)
                                         ggATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTTCTGTTAt                                                             >  2:1515416/1‑99 (MQ=255)
                                         ggATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTAtt                                                            <  2:327391/100‑1 (MQ=255)
                                          gATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTc                                                           >  2:2708075/1‑100 (MQ=255)
                                           aTTTTCTCCAGCTTCTGTATTGGTAATCAACCGCGCTTACGAATCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTa                                                              <  1:3141957/96‑1 (MQ=255)
                                           aTTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTcc                                                          <  1:626121/100‑1 (MQ=255)
                                                ctcCCGCTTCTGTATTAGTAAGAAACCACGCTTACGAAGCCGCCTTCTGACTGTCAGATGCGGCTTCGCTTAATCGTCACGACTCCTGTTATTCATCaa                                                      >  1:4017809/1‑99 (MQ=255)
                                                ctcCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGACGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAAc                                                     >  1:2354932/1‑100 (MQ=255)
                                                ctcCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAAc                                                     <  2:914434/100‑1 (MQ=255)
                                                 tcCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAAcc                                                    >  2:3529209/1‑100 (MQ=255)
                                                 tcCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAAcc                                                    >  2:3812979/1‑100 (MQ=255)
                                                 tcCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAAcc                                                    >  1:2841924/1‑100 (MQ=255)
                                                   cAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCt                                                  >  2:4266285/1‑100 (MQ=255)
                                                     gCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCttt                                                >  2:2585815/1‑100 (MQ=255)
                                                      cTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCtttt                                               <  1:3706460/100‑1 (MQ=255)
                                                      cTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCtttt                                               <  2:1799772/100‑1 (MQ=255)
                                                        tCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCtttttt                                             >  1:2767805/1‑100 (MQ=255)
                                                          tGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTTATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTa                                           <  1:693507/100‑1 (MQ=255)
                                                           gTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTaa                                          >  2:3373961/1‑100 (MQ=255)
                                                           gTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTaa                                          >  2:63558/1‑100 (MQ=255)
                                                           gTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTaa                                          <  2:108038/100‑1 (MQ=255)
                                                            tATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTaaa                                         <  2:3622588/100‑1 (MQ=255)
                                                             aTTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAAc                                        <  2:1200521/100‑1 (MQ=255)
                                                                ggTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACAtt                                     >  1:121311/1‑100 (MQ=255)
                                                                  taagtaaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTaa                                   <  1:2585605/100‑1 (MQ=255)
                                                                    agtaaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTaaaa                                 >  2:4057053/1‑100 (MQ=255)
                                                                       aaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCTGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTc                              <  2:1142083/100‑1 (MQ=255)
                                                                        cttccgatctcGAAGCCGCATCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCt                             <  1:1111218/90‑1 (MQ=25)
                                                                        aCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCt                             >  2:2409961/1‑100 (MQ=255)
                                                                          cgcgCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTa                           <  2:900266/100‑1 (MQ=255)
                                                                          cgcgCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTa                           >  1:1991595/1‑100 (MQ=255)
                                                                          cgcgCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTAAACATTAAAATTCTTAc                          <  2:4396615/100‑1 (MQ=255)
                                                                             gcTTACGATGCCTCATTCTGACTGTGATATGCGGGTTCGCTTCATTGTTACCACTACTGTTATTCCTGAACCCTGTTTTTAAACATTAAAATTCt                             <  1:4219833/95‑1 (MQ=255)
                                                                              cTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTa                       <  1:1801982/100‑1 (MQ=255)
                                                                                tACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAAt                     <  1:3543952/100‑1 (MQ=255)
                                                                                tACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAAt                     >  2:3418023/1‑100 (MQ=255)
                                                                                  cGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAAttt                   >  2:4128246/1‑100 (MQ=255)
                                                                                  cGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAAttt                   >  1:1769561/1‑100 (MQ=255)
                                                                                   gAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTta                  <  1:2485725/100‑1 (MQ=255)
                                                                                   gAAGCCGCATCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAAttt                   >  2:2019889/1‑99 (MQ=37)
                                                                                    aaGCCGCATCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTtat                 >  2:4211198/1‑100 (MQ=38)
                                                                                     aGCCGCATCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTACTTtata                >  1:3638590/1‑100 (MQ=38)
                                                                                     aGCCGCATCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTtata                >  2:2787428/1‑100 (MQ=38)
                                                                                      gCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTtata                <  1:554335/99‑1 (MQ=255)
                                                                                      gCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATaa               >  1:4386646/1‑100 (MQ=255)
                                                                                      gCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATaa               >  2:2397971/1‑100 (MQ=255)
                                                                                       ccGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAAt              <  2:3002781/100‑1 (MQ=255)
                                                                                         gCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCt            <  1:2924399/100‑1 (MQ=255)
                                                                                         gCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCt            >  2:237882/1‑100 (MQ=255)
                                                                                         gCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCt            <  1:4071962/100‑1 (MQ=255)
                                                                                         gCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCt            >  1:4359009/1‑100 (MQ=255)
                                                                                         gCATTCTGACTCTCAGATGTCTCTTCGCTTCATTGTTACCATTACTGTTATTCCTTAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAAt              <  1:3519556/98‑1 (MQ=255)
                                                                                           aTTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCttt          <  2:3945938/100‑1 (MQ=255)
                                                                                             tCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTaa        <  2:1731906/100‑1 (MQ=255)
                                                                                             tCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTa         >  2:1850659/1‑100 (MQ=255)
                                                                                              cTGACTGTCAGATGCGGCTTCGCTTTATTGTTACCATTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTaaa       <  1:3892739/100‑1 (MQ=255)
                                                                                              cTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTTTATTTTTTTATTCTTACGTAATTTATAATCTTTaaa       >  1:811642/1‑100 (MQ=255)
                                                                                                  cTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTaaaaaaa   <  2:1101571/100‑1 (MQ=255)
                                                                                                   tGTCAGATGCGGCTTCGCTTCATTGTTCCCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAg  <  1:2626548/100‑1 (MQ=255)
                                                                                                   tGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAg  <  2:3966688/100‑1 (MQ=255)
                                                                                                   tGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAg  <  2:1672930/100‑1 (MQ=255)
                                                                                                   |                                                                                                    
CGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAG  >  NC_000913/3888120‑3888318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: