Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,920,796 T→C 15.8% A31A (GCA→GCG atpF ← F0 sector of membrane‑bound ATP synthase, subunit b

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,920,7960TC15.8% 588.2 / 96.4 254A31A (GCA→GCGatpFF0 sector of membrane‑bound ATP synthase, subunit b
Reads supporting (aligned to +/- strand):  ref base T (100/113);  new base C (18/22);  total (118/136)
Fisher's exact test for biased strand distribution p-value = 8.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.84e-01

Only 100 of 255 total aligned reads displayed.

TGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAA  >  NC_000913/3920698‑3920895
                                                                                                  |                                                                                                   
tGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTg                                                                                                    <  1:3191592/100‑1 (MQ=255)
tGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTg                                                                                                    <  1:2734971/100‑1 (MQ=255)
tGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTg                                                                                                    >  2:4007559/1‑100 (MQ=255)
 ggtcggtcGCGTTGGGCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTg                                                                                                    <  1:347766/99‑1 (MQ=255)
 ggtcggtcGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGc                                                                                                   >  1:855057/1‑100 (MQ=255)
 ggtcggtcGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGc                                                                                                   >  2:4078482/1‑100 (MQ=255)
  gtcggtcgCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGcc                                                                                                  >  2:1875185/1‑100 (MQ=255)
  gtcggtcgCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGcc                                                                                                  <  2:3711289/100‑1 (MQ=255)
    cggtcgCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGcc                                                                                                  >  1:2596952/1‑98 (MQ=255)
        cgcgCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAAt                                                                                            >  1:1153651/1‑100 (MQ=255)
         gcgcTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATg                                                                                           >  2:235561/1‑100 (MQ=255)
         gcgaTGGCTTTTGCAAGGTCAATGTCCTTATGTGCGCGTTCTGCGGAAGCAATGCCGTCAGCAATTTCTTTTTGGCGTTTTTCGATGGCTGCCATTAATg                                                                                           <  1:1371457/100‑1 (MQ=255)
          cgcTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATg                                                                                           <  1:3396030/99‑1 (MQ=255)
             tGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATggcgg                                                                                       <  2:2184880/100‑1 (MQ=255)
              ggCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATggcagc                                                                                      >  2:3695628/1‑97 (MQ=255)
              ggCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATggcagc                                                                                      <  1:161980/100‑1 (MQ=255)
               gCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGcc                                                                                     <  2:3140836/100‑1 (MQ=255)
               gCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGcc                                                                                     <  1:3695605/100‑1 (MQ=255)
                ccTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCa                                                                                    >  2:3895256/1‑100 (MQ=255)
                ccTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCa                                                                                    >  2:2683008/1‑100 (MQ=255)
                ccTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCa                                                                                    <  1:4330382/100‑1 (MQ=255)
                 cTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCAt                                                                                   <  2:1513536/100‑1 (MQ=255)
                 cTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAt                                                                                   <  2:765324/100‑1 (MQ=255)
                  tttGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGTCGTTTTTCGATGGCTGCCATTAATGGCGGCCATa                                                                                  <  2:4125384/100‑1 (MQ=255)
                  tttGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAtc                                                                                  <  2:1275672/100‑2 (MQ=255)
                  tttGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAtc                                                                                  <  1:3199734/100‑2 (MQ=255)
                  tttGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAtc                                                                                  <  2:1192359/100‑2 (MQ=255)
                   ttGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAtcg                                                                                 <  1:3331561/100‑3 (MQ=255)
                   ctGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATAc                                                                                 <  1:4186394/99‑1 (MQ=255)
                    tGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAtcga                                                                                <  2:2334232/100‑4 (MQ=255)
                     gCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATAcag                                                                               >  2:4186424/1‑98 (MQ=255)
                     gCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGAAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACg                                                                                >  2:3109865/1‑99 (MQ=255)
                      caaggtcaaggtCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTa                                                                              >  1:2540913/1‑100 (MQ=255)
                      caaggtcaaggtCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTa                                                                              >  1:2051047/1‑100 (MQ=255)
                        aggtcaaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCACTAATGGCGGCCAtcgaaaaa                                                                            <  1:472457/100‑8 (MQ=255)
                          gtcaaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTc                                                                          <  2:1593938/100‑1 (MQ=255)
                          gtcaaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTc                                                                          <  2:1617892/100‑1 (MQ=255)
                           tcaaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCTGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCa                                                                         >  1:989574/1‑100 (MQ=255)
                           tcaaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCa                                                                         <  1:3310127/100‑1 (MQ=255)
                           ccgatctcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCa                                                                         <  1:2843571/94‑1 (MQ=255)
                              aggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGc                                                                      <  2:1706479/100‑1 (MQ=255)
                               ggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGca                                                                     >  1:765023/1‑100 (MQ=255)
                                  ccTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCaagatcgg                                                                  >  2:2843587/1‑93 (MQ=255)
                                   cTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcagaac                                                                 <  2:1932443/100‑1 (MQ=255)
                                   cTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcagaac                                                                 >  1:55835/1‑100 (MQ=255)
                                     tATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcagaacag                                                               >  2:1320980/1‑100 (MQ=255)
                                      aTGTGCTCGTTCTGCGGAAGCAAGTCCGTCAGCATTTTCGTTTTGGCGTTTTTCGATGGCTGCCATTAATGGGGGCCATACGTACTTCATGcagaacaga                                                              <  1:3558831/100‑1 (MQ=255)
                                       tgtgCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcagatcggaa                                                             >  1:3016049/1‑94 (MQ=255)
                                        gtgCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCagaacagaac                                                            <  2:2540926/100‑1 (MQ=255)
                                        gtgCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCagaacagaac                                                            <  2:812819/100‑1 (MQ=255)
                                        gtgCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCagaacagaac                                                            <  1:1215217/100‑1 (MQ=255)
                                         tgCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAgaacg                                                           >  2:1032323/1‑100 (MQ=255)
                                         tgCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAAAAgaacg                                                           >  1:3367657/1‑100 (MQ=255)
                                          gCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAgaacga                                                          <  1:2746339/100‑1 (MQ=255)
                                          gCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAgaacga                                                          >  2:43723/1‑100 (MQ=255)
                                            tCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAgaacgaac                                                        >  2:3371242/1‑100 (MQ=255)
                                             cGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACa                                                       <  2:511767/100‑1 (MQ=255)
                                             cGTTCTGCGGAAGCAAGGCCGTCAGCAATGTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACa                                                       <  1:2639404/100‑1 (MQ=255)
                                              gTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAg                                                      >  2:3281529/1‑100 (MQ=255)
                                                tCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGa                                                    <  1:1813365/100‑1 (MQ=255)
                                                 cTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGAc                                                   <  2:2611683/100‑1 (MQ=255)
                                                   gCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACaa                                                 >  2:4314340/1‑100 (MQ=255)
                                                           aaGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtg                                         <  2:573380/100‑1 (MQ=255)
                                                           aaGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtg                                         <  1:3161011/100‑1 (MQ=255)
                                                             ggCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtggc                                       >  2:4260551/1‑100 (MQ=255)
                                                             ggCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtgg                                        >  2:195782/1‑99 (MQ=255)
                                                              gCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtggcc                                      <  2:902284/100‑1 (MQ=255)
                                                                  tCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtggcctggc                                  <  1:167197/100‑1 (MQ=255)
                                                                     ncAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGa                               >  1:242905/2‑100 (MQ=255)
                                                                     gCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGa                               <  1:905556/100‑1 (MQ=255)
                                                                     gCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGa                               >  2:785082/1‑100 (MQ=255)
                                                                       aaTTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAgg                             >  2:3432225/1‑100 (MQ=255)
                                                                        aTTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGa                            <  1:4078453/100‑1 (MQ=255)
                                                                           tCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAttg                         <  2:222078/100‑1 (MQ=255)
                                                                           tCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAttg                         <  2:3855019/100‑1 (MQ=255)
                                                                            cTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAttgt                        <  1:4261310/100‑1 (MQ=255)
                                                                              ttttGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAttgttn                      <  1:1701293/100‑2 (MQ=255)
                                                                              ttttGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAttgttg                      <  1:3098736/100‑1 (MQ=255)
                                                                              ttttGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAttgttg                      <  2:460240/100‑1 (MQ=255)
                                                                              ttttGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAttgttg                      >  1:1256139/1‑100 (MQ=255)
                                                                              ttttGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAttgttg                      <  1:1081087/100‑1 (MQ=255)
                                                                              ttttGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAttgttg                      <  2:1652718/100‑1 (MQ=255)
                                                                               tttGACGTTTTTCGATGGCTGCCATTAATGGCGGCCAAACGTACCTCATGCAGAACAGAACGAACAGGACAAACCCGATGACCTGGACGAGGATTGTTGc                     <  1:2266867/100‑1 (MQ=255)
                                                                                ttGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCg                    >  1:3578889/1‑100 (MQ=255)
                                                                                   aCGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTa                 <  2:2923613/100‑1 (MQ=255)
                                                                                   aCGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTa                 <  2:540875/100‑1 (MQ=255)
                                                                                    cGTTTTTCGATTTTTTCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTTCGTTaa                >  1:57455/1‑100 (MQ=255)
                                                                                    cGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTaa                <  2:3627528/100‑1 (MQ=255)
                                                                                    cGTTTTTCGATGGCTGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCAAGGATTGTTGCGTTaa                >  1:2578062/1‑100 (MQ=255)
                                                                                      tttttCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGa              <  1:3844211/100‑1 (MQ=255)
                                                                                      agtacgtaTGGCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGa              >  1:3711573/8‑100 (MQ=38)
                                                                                        tacgtaTGGCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGAtt            >  1:1932231/6‑100 (MQ=38)
                                                                                        tacgtaTGGCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGAtt            >  1:3068754/6‑100 (MQ=38)
                                                                                          tCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTca          >  2:3231777/1‑100 (MQ=255)
                                                                                            taTGGCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTcaca        >  2:724876/2‑100 (MQ=255)
                                                                                            taTGGCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTcaca        >  2:2503327/2‑100 (MQ=255)
                                                                                              tGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTcacacc      <  2:2970788/100‑3 (MQ=255)
                                                                                              tGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAg       <  1:1911888/99‑1 (MQ=255)
                                                                                              tGGCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGc      >  2:1358818/1‑100 (MQ=255)
                                                                                               ggCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGACCAGGACAAACGCGATGGCCTGGCCGAGGCTTGTTGCGTTAAGATTCACAGca     >  1:2882256/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
TGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAA  >  NC_000913/3920698‑3920895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: