Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,148,049 C→G 15.1% G90A (GGC→GCC)  yijO ← AraC family putative transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,148,0490CG15.1% 631.2 / 92.7 231G90A (GGC→GCC) yijOAraC family putative transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (95/100);  new base G (14/21);  total (110/121)
Fisher's exact test for biased strand distribution p-value = 3.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.07e-01

Only 100 of 242 total aligned reads displayed.

AACCTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTC  >  NC_000913/4147951‑4148148
                                                                                                  |                                                                                                   
 aCCTATGCGTGGGCCTCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCa                                                                                                   <  2:4339274/100‑1 (MQ=255)
  ccTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCaa                                                                                                  >  2:2062718/1‑100 (MQ=255)
  ccTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCaa                                                                                                  >  2:28910/1‑100 (MQ=255)
   cTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCaaa                                                                                                 >  2:2839091/1‑100 (MQ=255)
    tATGCGTGGGGCGCGTCAGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAAc                                                                                                <  1:1914669/100‑1 (MQ=255)
    tATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAAc                                                                                                <  2:1478598/100‑1 (MQ=255)
    tATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAAc                                                                                                <  1:2077424/100‑1 (MQ=255)
     aTGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACa                                                                                               <  2:3920553/100‑1 (MQ=255)
       gCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGGCAAACAGc                                                                                             <  1:3740823/100‑1 (MQ=255)
        cGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCa                                                                                            <  2:2690422/100‑1 (MQ=255)
        cGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCa                                                                                            <  2:531111/100‑1 (MQ=255)
        cGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCa                                                                                            >  2:4094773/1‑100 (MQ=255)
          tGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGGCAAACAGCaac                                                                                          <  2:1052416/100‑3 (MQ=255)
           gggCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGGCAAACAGCaact                                                                                         <  2:2809917/100‑4 (MQ=255)
            ggCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGGCAAACAGCaactc                                                                                        <  2:3736537/100‑5 (MQ=255)
            ggCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCt                                                                                        <  2:3216570/100‑1 (MQ=255)
             gCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGGCAAACAGCaactcg                                                                                       <  2:1793619/100‑6 (MQ=255)
             gCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGGCAAACAGCaactcg                                                                                       <  2:395657/100‑6 (MQ=255)
             gCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGGCAAACAGCaactcg                                                                                       <  1:3184442/100‑6 (MQ=255)
              ccGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGGCAAACAGCaactcgg                                                                                      <  2:3250769/100‑7 (MQ=255)
              ccGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGGCAAACAGCaactcgg                                                                                      <  2:782716/100‑7 (MQ=255)
              ccGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGGCAAACAGCaactcgg                                                                                      <  1:3231283/100‑7 (MQ=255)
              ccGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGGTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGGCAAACAGCaactcgg                                                                                      <  2:2622593/100‑7 (MQ=255)
                gcgTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAg                                                                                    <  1:67541/100‑1 (MQ=255)
                gcgTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAg                                                                                    >  2:1602988/1‑100 (MQ=255)
                gcgACGGGCGACGTGTTGCTTCGCCAGATTTTGATCTTGTTTGCCATCCCGTTGCACGCCGCTGAAGCCGAGTGGCTGTTTGGCAAACAGCaactcggct                                                                                    <  1:1053736/100‑9 (MQ=37)
                 cgTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAgg                                                                                   <  1:2374777/100‑1 (MQ=255)
                 cgTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAgg                                                                                   >  2:548932/1‑100 (MQ=255)
                   tCGGGGGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCTATTCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGGCAAACAGCaactcggct                                                                                    <  1:2826604/97‑9 (MQ=37)
                    cGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAgatcg                                                                                >  2:3530257/1‑96 (MQ=255)
                     gggCGACGTGTTGCTTCGCCAGATTTTGAGATTGTTTGCCATTCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGgtag                                                                                <  1:229309/99‑1 (MQ=255)
                       gCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATGGCACGACGCTGAAGACGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGtagtag                                                                             >  1:1570329/1‑100 (MQ=255)
                            gtgtTGCTTCGCCAGATTTTGATCTTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCgggg                                                                        <  1:4268248/100‑1 (MQ=255)
                             tgtTGCTTCGCCAGATTTTGATATTGTTTGCCGTCCCATTGCACGACGCTGAAGCCGATTTGCTGTTTGCCGCACAGCACGCAAAAGgtagtat                                                                             >  1:65008/1‑93 (MQ=255)
                             tgtTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCggggg                                                                       >  2:3542842/1‑100 (MQ=255)
                                  cTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGc                                                                  <  2:3260328/100‑1 (MQ=255)
                                  cTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGc                                                                  <  1:1601468/100‑1 (MQ=255)
                                  cTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGc                                                                  <  1:3687963/100‑1 (MQ=255)
                                   ttCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGcc                                                                 >  1:2228141/1‑100 (MQ=255)
                                    tccccAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCa                                                                <  1:2774977/97‑1 (MQ=255)
                                     cGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCAt                                                               <  1:356981/100‑1 (MQ=255)
                                      gCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCAtt                                                              >  2:685292/1‑100 (MQ=255)
                                      gCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCAtt                                                              >  2:3790161/1‑100 (MQ=255)
                                       ccAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATtg                                                             >  2:1805114/1‑100 (MQ=255)
                                        cAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATtgt                                                            <  2:4188987/100‑1 (MQ=255)
                                         ggATTTTGATGTTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATtgtg                                                           <  1:4025683/99‑1 (MQ=255)
                                         aGATTTTGAGATGGGTTGCCAGCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATtgtg                                                           <  1:2417042/100‑1 (MQ=255)
                                             tttGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGaaa                                                       >  2:2173231/1‑100 (MQ=255)
                                             tttGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGaaa                                                       >  2:1267433/1‑100 (MQ=255)
                                               tGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGGAGCGGGGGCTTGCCATTGTGGAAAAt                                                     <  2:2671978/100‑1 (MQ=255)
                                                gATATTGTGTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAAtt                                                    <  1:1685362/100‑1 (MQ=255)
                                                    ttGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAg                                                <  2:3879335/100‑1 (MQ=255)
                                                    ttGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAg                                                <  2:1769779/100‑1 (MQ=255)
                                                    ttGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAg                                                <  1:2729751/100‑1 (MQ=255)
                                                     tGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCa                                                 <  1:3845294/98‑1 (MQ=255)
                                                     tGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGc                                               >  1:1833765/1‑100 (MQ=255)
                                                      gTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGCAAATTCCAGcc                                              >  1:1804117/1‑100 (MQ=255)
                                                      gTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGcc                                              >  2:1347655/1‑100 (MQ=255)
                                                      gTTTGCCATCCCATTGCACGAAGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAg                                                >  1:497849/1‑98 (MQ=255)
                                                       tttGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGcca                                             <  2:4061070/100‑1 (MQ=255)
                                                         tGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGccacc                                           >  2:1474058/1‑100 (MQ=255)
                                                           ccATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTAGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACcag                                         >  1:2354741/1‑100 (MQ=255)
                                                             aTCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACcagca                                       <  1:830962/100‑1 (MQ=255)
                                                                   ttGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACg                                 <  2:3667866/100‑1 (MQ=255)
                                                                     gCACGACGCTGAAGCCGAGTTGCTGTTTGTCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTa                               >  1:3139607/1‑100 (MQ=255)
                                                                     gCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTa                               >  1:2499243/1‑100 (MQ=255)
                                                                       acgacgCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACa                             >  1:60301/1‑100 (MQ=255)
                                                                        cgacgCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAg                            <  2:1980505/100‑1 (MQ=255)
                                                                         gacgCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGc                           <  1:3425896/100‑1 (MQ=255)
                                                                         gacgCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGc                           <  2:448344/100‑1 (MQ=255)
                                                                         gacgCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGc                           <  2:313161/100‑1 (MQ=255)
                                                                         gacgCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGc                           >  2:481933/1‑100 (MQ=255)
                                                                          acgCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGca                          <  1:759194/100‑1 (MQ=255)
                                                                            gCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGcaca                        <  2:1550629/100‑1 (MQ=255)
                                                                             cTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCTGGGGCTTGCCAGTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACAt                       >  1:3506972/1‑100 (MQ=255)
                                                                             cTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACAt                       >  2:2346292/1‑100 (MQ=255)
                                                                              tGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATc                      >  2:4017702/1‑100 (MQ=255)
                                                                              tGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGGGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATc                      >  2:3072037/1‑100 (MQ=255)
                                                                               gAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATc                      >  1:3656524/1‑99 (MQ=255)
                                                                                 aGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGcc                   >  2:1111950/1‑100 (MQ=255)
                                                                                 aGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGGAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACAGCGcc                   >  1:3893252/1‑100 (MQ=255)
                                                                                   ccGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCgg                 <  1:1204549/100‑1 (MQ=255)
                                                                                    cGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCggg                >  1:3867245/1‑100 (MQ=255)
                                                                                    cGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCggg                <  2:3404423/100‑1 (MQ=255)
                                                                                    cGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCggg                >  2:4234151/1‑100 (MQ=255)
                                                                                      gcgtGCTGTTTGGCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACATCACATCCCCg                  >  1:2581358/4‑96 (MQ=255)
                                                                                      gcgtGCTGTTTGGCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGaa              >  1:1242845/4‑100 (MQ=255)
                                                                                      aGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGaa              <  1:1978074/100‑1 (MQ=255)
                                                                                       gTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAAc             <  2:122513/100‑1 (MQ=255)
                                                                                       cgtGCTGTTTGGCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAAc             >  1:1121831/3‑100 (MQ=255)
                                                                                        gtGCTGTTTGGCAAACAGCACGCTAAAGGTAGTAGCAGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACt            >  2:824385/2‑100 (MQ=255)
                                                                                        gtGCTGTTNGGCAAACAGCACGCTAANGGTANTANCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGCACGTACAGCACATCGCCNGGAACt            >  2:3848824/2‑100 (MQ=255)
                                                                                          gCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTaa          >  2:644989/1‑100 (MQ=255)
                                                                                           cTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAAc         >  2:308970/1‑100 (MQ=255)
                                                                                            tGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTaa          >  1:1002468/1‑98 (MQ=255)
                                                                                             gTTTGCCAAACAGCACGCTAAATGTAGTAGCGGGGGCTTGCCATTGTAGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGt       <  1:3110347/100‑1 (MQ=255)
                                                                                               ttGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTg     <  1:2000097/100‑1 (MQ=255)
                                                                                                 gCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCt   >  1:4369484/1‑100 (MQ=255)
                                                                                                  ccAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTc  >  1:177047/1‑100 (MQ=255)
                                                                                                  ccAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCAATGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTc  <  1:817015/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
AACCTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGGGCTTGCCATTGTGGAAAATTCCAGCCACCAGCAGGAACGTACAGCACATCGCCGGGAACTAACGTTGCTC  >  NC_000913/4147951‑4148148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: