Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,312,083 G→C 15.1% intergenic (‑41/+18) alsB ← / ← alsR D‑allose ABC transporter periplasmic binding protein/d‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,312,0830GC15.1% 583.8 / 92.6 219intergenic (‑41/+18)alsB/alsRD‑allose ABC transporter periplasmic binding protein/d‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR
Reads supporting (aligned to +/- strand):  ref base G (86/99);  new base C (17/16);  total (103/116)
Fisher's exact test for biased strand distribution p-value = 7.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.41e-01

Only 100 of 224 total aligned reads displayed.

TGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGC  >  NC_000913/4311985‑4312182
                                                                                                  |                                                                                                   
tGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATgg                                                                                                    <  2:2481956/100‑1 (MQ=255)
 ngTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGc                                                                                                   >  1:2273990/2‑100 (MQ=255)
   ttGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACGGGTAACTCCGCGATGGCgg                                                                                                 <  2:2570230/100‑1 (MQ=255)
   ttGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCgg                                                                                                 <  1:1074490/100‑1 (MQ=255)
     gACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGaa                                                                                               <  2:1047496/100‑1 (MQ=255)
      acaacaTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAAt                                                                                              <  2:3512232/100‑1 (MQ=255)
       caacaTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAAtt                                                                                             >  2:1246531/1‑100 (MQ=255)
       caacaTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAAtt                                                                                             >  1:2238003/1‑100 (MQ=255)
       caacaTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAAtt                                                                                             <  2:3320649/100‑1 (MQ=255)
         acaTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTAc                                                                                           >  2:666703/1‑100 (MQ=255)
          caTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTAcc                                                                                          <  2:1459434/100‑1 (MQ=38)
          caTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTAcc                                                                                          <  1:3685986/100‑1 (MQ=255)
           aTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCt                                                                                         <  2:4282058/100‑1 (MQ=255)
            ttAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtg                                                                                        <  1:607590/100‑2 (MQ=38)
            ttAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtg                                                                                        <  2:529593/100‑2 (MQ=38)
            ttAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCtt                                                                                        >  1:4353210/1‑100 (MQ=255)
             tAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtg                                                                                        <  1:4103874/99‑2 (MQ=38)
             tAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtg                                                                                        <  2:1668347/99‑2 (MQ=38)
               aGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtgtcc                                                                                     <  1:3630680/100‑5 (MQ=37)
                gtccACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTc                                                                                    <  1:4346771/98‑1 (MQ=255)
                gCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTc                                                                                    >  2:2933926/1‑100 (MQ=255)
                 cccACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCCCGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACATTTTTCt                                                                                   >  1:352043/1‑100 (MQ=255)
                 cccACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCt                                                                                   <  1:679184/100‑1 (MQ=255)
                   cACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTa                                                                                 >  2:803636/1‑100 (MQ=255)
                    aCGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTAt                                                                                <  1:907340/100‑1 (MQ=255)
                     cGAGTGTGTCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGTACAGGTAACTCCGCGATTGCGGAATTACCTTTTTCTTAtt                                                                               <  1:4391078/100‑1 (MQ=255)
                       aGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTAtt                                                                               <  1:3155509/98‑1 (MQ=255)
                       aGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTc                                                                             <  1:1734129/100‑1 (MQ=255)
                        gtgtGCCGCTTAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTc                                                                             <  2:4272272/99‑1 (MQ=255)
                          gtgCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgc                                                                          <  1:115125/100‑1 (MQ=255)
                          gtgCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgc                                                                          <  1:1473304/100‑1 (MQ=255)
                             gcGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgcgcg                                                                       <  1:453340/99‑1 (MQ=255)
                               gCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgcgcgc                                                                      <  1:2792622/99‑1 (MQ=255)
                               gCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGcc                                                                     <  2:2022872/100‑1 (MQ=255)
                               gCTGAAATATTTCAGATATTTATTCATAATAATGTTCGCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgcgcgcn                                                                     <  1:1423264/100‑2 (MQ=255)
                                cTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCt                                                                    >  2:410697/1‑100 (MQ=255)
                                   aaaTATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGcc                                                                     <  1:1304220/96‑1 (MQ=255)
                                      tatTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTgag                                                              <  2:790270/100‑1 (MQ=255)
                                       atTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCTGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTgaga                                                             >  2:2631783/1‑100 (MQ=255)
                                        tttCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGagaa                                                            <  1:847048/100‑1 (MQ=255)
                                         ttCAGATATTTATTCATAATAATGTTCTCACGGTAATGGGACAGGTAACTCCGCTATGGCGTAATTACCTTTTTCTTATTTCATCGCGCCTGGTGagaag                                                           >  2:1299380/1‑100 (MQ=255)
                                           cAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAgaagaa                                                         >  2:4398959/1‑100 (MQ=255)
                                           cAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAgaagaa                                                         >  1:1643186/1‑100 (MQ=255)
                                              atatTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATc                                                      <  1:1389467/100‑1 (MQ=255)
                                               tatttattCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATca                                                     >  2:960975/1‑100 (MQ=255)
                                               tatttattCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATca                                                     >  1:4186494/1‑100 (MQ=255)
                                                atttattCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATcaa                                                    <  1:2580671/100‑1 (MQ=255)
                                                atttattCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATcaa                                                    <  1:1521764/100‑1 (MQ=255)
                                                    attCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATcaacaa                                                 >  2:2824567/1‑99 (MQ=255)
                                                      tCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTg                                              <  2:1322741/100‑1 (MQ=255)
                                                       cATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTgc                                             >  2:2297442/1‑100 (MQ=255)
                                                        ataataATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTgc                                             >  2:1407118/1‑99 (MQ=255)
                                                        ataataATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTgcgc                                           <  1:2536802/100‑1 (MQ=255)
                                                         taataaTGTTCTCACGGTAAGGGGACAGGCAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGGGAAGAAATCAACAATTgcgc                                           >  1:563515/1‑100 (MQ=255)
                                                                  tctcACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGtttttt                                  >  1:1243095/1‑100 (MQ=255)
                                                                   tctaCGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTg                                 <  1:824573/97‑1 (MQ=255)
                                                                   ctcACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTg                                 <  1:2481132/100‑1 (MQ=255)
                                                                   ctcACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTg                                 <  2:4220522/100‑1 (MQ=255)
                                                                      aCGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCat                              <  2:2246978/100‑1 (MQ=255)
                                                                        ggTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTgagatc                            >  1:3045441/1‑95 (MQ=255)
                                                                         gTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatatt                           >  1:3322158/1‑100 (MQ=255)
                                                                          tAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatatta                          >  1:177059/1‑100 (MQ=255)
                                                                           taagtataaGGTAATTCCGCCTTCGCGTAGTCACTTTTTTCTTATTTCAGCGCGACTGGTGAGCAGAAATCTACAATTGCGTCGGTTTTTTTCatat                            >  1:2952869/9‑97 (MQ=255)
                                                                            aGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaat                        <  2:1776762/100‑1 (MQ=255)
                                                                            aGGGGACAGGTAACTCCGCGATGGCGGAATTACATTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaat                        >  1:3843499/1‑100 (MQ=255)
                                                                             agaaaaaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaata                       >  2:2894411/7‑100 (MQ=25)
                                                                              gggACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaatat                      >  1:1594241/1‑100 (MQ=255)
                                                                              gggACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaata                       <  2:3670789/99‑1 (MQ=255)
                                                                                gACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaatatta                    <  2:174220/100‑1 (MQ=255)
                                                                                gACAGGTAACTCCGCGATGGCGGAATTACCTTTTCCTTACTTCAGCGCGCCTGGTGAGAAGAAAGCAAAAAGTGCGCCGGTCTTTTGCataataatatta                    >  1:1696022/1‑100 (MQ=255)
                                                                                 aaaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaatatt                     >  1:3397818/3‑98 (MQ=37)
                                                                                 aaaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAg                   >  1:1000544/3‑100 (MQ=38)
                                                                                 aCAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAg                   <  2:3600425/100‑1 (MQ=255)
                                                                                  ccggTAACTCCGCGATGACGGAATTAACTTTTTCTTAATTCAACGACCCTGGAGAAAATAAATCAACAATTACGCAGGATATTTCCatatta                          >  1:1987073/3‑92 (MQ=255)
                                                                                  cAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGc                  <  2:3237940/100‑1 (MQ=255)
                                                                                  aaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGc                  >  2:2199948/2‑100 (MQ=38)
                                                                                  aaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGc                  >  1:1611678/2‑100 (MQ=38)
                                                                                  aaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGc                  >  2:737807/2‑100 (MQ=38)
                                                                                   nggTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCt                 >  2:164522/2‑100 (MQ=38)
                                                                                   aGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTTCATATTAATATTAg                   >  1:483373/1‑98 (MQ=38)
                                                                                   aGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGAGCCGGTTTTTTGCATATTAATATTAGCt                 >  2:1546467/1‑100 (MQ=38)
                                                                                   aGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCTCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCt                 <  1:399112/100‑1 (MQ=255)
                                                                                   aGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCt                 <  2:3037993/100‑1 (MQ=255)
                                                                                   aGGTAACTCCGCGATGGCGGAATTACCTTGTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACGATTGCGCCGGTTTTTTGCATATTAATATTAGCt                 <  1:2640075/100‑1 (MQ=255)
                                                                                    ggTAATTTCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAATTCAACAATTGCGCAGGTTTTTTTCATATTAATCTTAACtt                >  1:3696822/1‑100 (MQ=255)
                                                                                    ggTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCtt                >  1:139554/1‑100 (MQ=38)
                                                                                    ggTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCt                 >  2:153595/1‑99 (MQ=255)
                                                                                       aaCTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGtt             >  2:3742531/1‑100 (MQ=255)
                                                                                         cTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTc            <  2:1285417/99‑1 (MQ=255)
                                                                                           ccGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAAt         >  2:2469371/1‑100 (MQ=255)
                                                                                            cgcgATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATg        <  1:2100037/100‑1 (MQ=255)
                                                                                            cgcgATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATg        <  2:2748741/100‑1 (MQ=255)
                                                                                            cgcgATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATg        <  1:525100/100‑1 (MQ=255)
                                                                                             gcgATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAAt         <  1:515103/98‑1 (MQ=255)
                                                                                             gcgATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGt       <  2:3395924/100‑1 (MQ=255)
                                                                                             gcgATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGt       <  1:2881400/100‑1 (MQ=255)
                                                                                               gATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTg     <  2:4353241/100‑1 (MQ=255)
                                                                                               gATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTg     <  2:1060183/100‑1 (MQ=255)
                                                                                               gATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTg     >  1:1101635/1‑100 (MQ=255)
                                                                                                  ggCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGc  <  2:2856093/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
TGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGC  >  NC_000913/4311985‑4312182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: