Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,470,958 G→T 16.4% intergenic (‑45/+28) ridA ← / ← pyrI enamine/imine deaminase, reaction intermediate detoxification/aspartate carbamoyltransferase, regulatory subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,470,9580GT16.4% 510.8 / 81.4 202intergenic (‑45/+28)ridA/pyrIenamine/imine deaminase, reaction intermediate detoxification/aspartate carbamoyltransferase, regulatory subunit
Reads supporting (aligned to +/- strand):  ref base G (85/82);  new base T (19/14);  total (105/97)
Fisher's exact test for biased strand distribution p-value = 5.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-01

Only 100 of 216 total aligned reads displayed.

GTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGG  >  NC_000913/4470859‑4471056
                                                                                                   |                                                                                                  
gTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAgg                                                                                                    <  2:3393507/100‑1 (MQ=255)
gTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAgg                                                                                                    <  2:2337495/100‑1 (MQ=255)
 tACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAggg                                                                                                   >  1:3563687/1‑100 (MQ=255)
  aCGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGa                                                                                                  <  2:896775/100‑1 (MQ=255)
   cGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAg                                                                                                 <  2:4014937/100‑1 (MQ=255)
   cGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAg                                                                                                 >  2:298796/1‑100 (MQ=255)
    gTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAGAGTCTGGCTCATTATAGGGAg                                                                                                 >  1:1027362/1‑99 (MQ=255)
     tAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTCAAAAGACTGGCTCATTATAgg                                                                                                    >  1:668706/1‑95 (MQ=255)
     tAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGcc                                                                                               >  2:4300687/1‑100 (MQ=255)
     tAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGcc                                                                                               <  2:4332448/100‑1 (MQ=255)
     tAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGcc                                                                                               >  1:2208662/1‑100 (MQ=255)
        ggACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGa                                                                                            >  2:1975306/1‑100 (MQ=255)
         gACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGCCAGAc                                                                                           <  2:1021832/100‑1 (MQ=38)
          aCCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGCCAGACt                                                                                          <  2:3100518/100‑1 (MQ=38)
          aCCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACt                                                                                          <  1:2739939/100‑1 (MQ=255)
           ccGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGCCAGACtt                                                                                         <  1:2862319/100‑1 (MQ=38)
           ccGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACtt                                                                                         >  2:2557021/1‑100 (MQ=255)
            cGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTCCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGCCAGAc                                                                                           <  1:4323692/97‑1 (MQ=38)
             gATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACtttt                                                                                       >  1:598332/1‑100 (MQ=255)
                aGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGCCAGACtttttac                                                                                    <  1:1960552/100‑4 (MQ=37)
                 gCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGCCAGACtttttacc                                                                                   <  2:4309045/100‑5 (MQ=25)
                  cTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTttattan                                                                                  <  1:523722/100‑2 (MQ=255)
                  cTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTAc                                                                                  <  2:2218711/100‑1 (MQ=255)
                   tGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTAcc                                                                                 >  1:1149335/1‑100 (MQ=255)
                   tGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTAcc                                                                                 >  2:2179192/1‑100 (MQ=255)
                   tGCCGGTGCATTTTCCGTCGCGATAGTTTTGCCCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTAcc                                                                                 <  2:178963/100‑1 (MQ=255)
                     ccGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCaa                                                                               >  1:2359909/1‑100 (MQ=255)
                     ccGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTGATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCaa                                                                               <  1:614222/100‑1 (MQ=255)
                       ggTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAAcc                                                                             <  2:3331362/100‑1 (MQ=255)
                         tGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGc                                                                           <  2:168692/100‑1 (MQ=255)
                            aTTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATATGGAGCCAGACTTTTATTACCAACCGCaat                                                                        >  1:4267025/1‑100 (MQ=255)
                            aTTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCaat                                                                        >  2:4003587/1‑100 (MQ=255)
                            aTTTTCCGTCGCGATAGTTTTGCTCATGATATCACCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTATCAACCGCaat                                                                        >  1:2890031/1‑100 (MQ=255)
                             ttttCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCaatt                                                                       <  2:1212043/100‑1 (MQ=255)
                               ttCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCaattaa                                                                     <  2:240674/100‑1 (MQ=255)
                                tCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCaattaat                                                                    >  1:2494310/1‑100 (MQ=255)
                                 ccGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGTAGCCAGACTTTTATTACCAACCGCaattaatt                                                                   <  2:4181341/100‑1 (MQ=255)
                                     cgcgATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACAACCGCAATTAATTGGCCa                                                              <  1:1165083/100‑1 (MQ=255)
                                       cgATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAg                                                             >  2:1637399/1‑100 (MQ=255)
                                       cgATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAg                                                             >  1:2412013/1‑100 (MQ=255)
                                       caaTAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAg                                                             >  2:2966381/3‑100 (MQ=255)
                                        gATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGc                                                            >  1:3662970/1‑100 (MQ=255)
                                        gATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGc                                                            <  2:1329336/100‑1 (MQ=255)
                                            gTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGcacca                                                        >  2:589896/1‑100 (MQ=255)
                                              tttGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGcaccac                                                       >  1:3440687/1‑99 (MQ=255)
                                                 gCTCATGATTTCTCCTTTATTACATCAGTAATAAGTCTGGCTCATTATAGGGAGCCAGCCTTTTATTACCAACCTCAATTTCTTGGCCAGCTCCACAtca                                                   <  1:586448/100‑3 (MQ=255)
                                                  cTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTAt                                                  >  1:1856806/1‑100 (MQ=255)
                                                   tCATGATTTCTCCTTTATTAAAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTAt                                                  >  1:2686506/1‑99 (MQ=255)
                                                    cATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATgg                                                <  2:3814553/100‑1 (MQ=255)
                                                    cATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATgg                                                >  2:780625/1‑100 (MQ=255)
                                                        aTTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGaaa                                            >  2:653418/1‑100 (MQ=255)
                                                         tttCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGaaaa                                           >  2:1855951/1‑100 (MQ=255)
                                                             tcCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAActct                                       >  2:1725937/1‑100 (MQ=255)
                                                                tttATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCtttt                                    >  1:1090767/1‑100 (MQ=255)
                                                                  tattaCAGCGGTAAAAAGTCTGGCTCATTATATGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTc                                  <  1:391305/100‑1 (MQ=255)
                                                                  tattaCAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTc                                  <  2:3320582/100‑1 (MQ=255)
                                                                    ttaCAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTcac                                <  2:861179/100‑1 (MQ=255)
                                                                    ttaCAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTcac                                >  1:3435710/1‑100 (MQ=255)
                                                                    ttaCAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTcac                                <  2:3854/100‑1 (MQ=255)
                                                                    ttaCAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTcac                                <  1:1264840/100‑1 (MQ=255)
                                                                     taCAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTcaca                               <  1:3886543/100‑1 (MQ=255)
                                                                     taCAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTcaca                               >  1:1601724/1‑100 (MQ=255)
                                                                      aCAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTGTGGGAAAACTCTTTTTCACAg                              <  2:3140367/100‑1 (MQ=255)
                                                                       cAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGt                             <  2:3425566/100‑1 (MQ=255)
                                                                            gTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTg                        <  1:4195421/100‑1 (MQ=255)
                                                                            gTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTg                        <  2:2656637/100‑1 (MQ=255)
                                                                            gTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTg                        <  2:2883672/100‑1 (MQ=255)
                                                                            aataaaaGTCTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTg                        >  1:918450/4‑100 (MQ=37)
                                                                             tAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGc                       <  2:3493535/100‑1 (MQ=255)
                                                                              taaaaGTCTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCa                      >  2:1452612/2‑100 (MQ=37)
                                                                              taaaaGTCTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCa                      >  2:3188375/2‑100 (MQ=37)
                                                                              taaaaGTCTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCa                      >  1:3811847/2‑100 (MQ=37)
                                                                              aaaaaGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCa                      <  2:3615249/100‑1 (MQ=255)
                                                                              aaaaaGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCa                      >  2:1225057/1‑100 (MQ=255)
                                                                                aaaGTCTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCAtt                    >  1:1754207/1‑100 (MQ=38)
                                                                                aaaGTCTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATATGCAtt                    >  1:3813583/1‑100 (MQ=37)
                                                                                aaaGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCAtt                    <  1:2990303/100‑1 (MQ=255)
                                                                                 aaGTCTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCAttt                   >  2:2491883/1‑100 (MQ=38)
                                                                                 aaGTCTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTAGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCAtt                    >  1:4221976/1‑99 (MQ=37)
                                                                                 aaGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCAttt                   >  2:2093004/1‑100 (MQ=255)
                                                                                  aGTCTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCAttt                   >  1:2083294/1‑99 (MQ=38)
                                                                                  aGTCTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTg                  >  2:530098/1‑100 (MQ=38)
                                                                                  aGTCTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTg                  >  1:2855697/1‑100 (MQ=38)
                                                                                  aGTCTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTACATTTg                  >  1:573023/1‑100 (MQ=37)
                                                                                  aGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTg                  <  2:3905469/100‑1 (MQ=255)
                                                                                    tCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAg                <  1:3068239/100‑1 (MQ=255)
                                                                                     cTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAgc               >  2:2902702/1‑100 (MQ=255)
                                                                                        gCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGcgcg            <  1:3841277/100‑1 (MQ=255)
                                                                                        gCTCATTATAGGGAGCCACACTTTTATTACCAACAGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAg                <  1:1994485/96‑1 (MQ=255)
                                                                                         cTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGa           <  2:1532470/100‑1 (MQ=255)
                                                                                         cTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGa           >  2:3311048/1‑100 (MQ=255)
                                                                                         cTCATTATAGGGAGCCAGACTTTTATTACAAACCACAATTAATAAAAAAGCAGCACAGTATGGGAAAACGCTTGTTCACAGTATTTGCGTGTGAGCGCGa           >  1:1458169/1‑100 (MQ=255)
                                                                                          tCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGat          >  2:2776075/1‑100 (MQ=255)
                                                                                           cATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGata         >  2:4331158/1‑100 (MQ=255)
                                                                                           cATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGata         >  1:1338005/1‑100 (MQ=255)
                                                                                                taGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCAtt    <  2:962629/100‑1 (MQ=255)
                                                                                                 aGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTg   <  2:3117307/100‑1 (MQ=255)
                                                                                                 aGGGAGCCAGACTTTACTTATCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTg   <  1:2571609/100‑1 (MQ=255)
                                                                                                  gggAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTgg  >  2:2176252/1‑100 (MQ=255)
                                                                                                  gggAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTgg  >  2:663780/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
GTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGG  >  NC_000913/4470859‑4471056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: