Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,549,932 T→A 16.8% intergenic (+222/+21) fimH → / ← gntP minor component of type 1 fimbriae/fructuronate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,549,9320TA16.8% 451.2 / 79.6 186intergenic (+222/+21)fimH/gntPminor component of type 1 fimbriae/fructuronate transporter
Reads supporting (aligned to +/- strand):  ref base T (78/76);  new base A (14/17);  total (92/94)
Fisher's exact test for biased strand distribution p-value = 6.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.55e-01

Only 100 of 189 total aligned reads displayed.

CTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTC  >  NC_000913/4549833‑4550031
                                                                                                   |                                                                                                   
 tttGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCAtt                                                                                                    <  2:1733009/100‑1 (MQ=255)
  ttGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTg                                                                                                   <  2:2516473/100‑1 (MQ=255)
    gCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGc                                                                                                 <  2:2618277/100‑1 (MQ=255)
     cTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCa                                                                                                <  2:3789724/100‑1 (MQ=255)
       ggTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAgg                                                                                              <  1:1051669/100‑1 (MQ=255)
       ggTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAgg                                                                                              <  2:1793387/100‑1 (MQ=255)
          cTGTTCCCCTCACTCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCAATGGCAGGctc                                                                                           <  1:747137/100‑1 (MQ=255)
          cTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGctc                                                                                           >  2:2559609/1‑100 (MQ=255)
          cTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCAATGGCAGGctc                                                                                           <  2:73783/100‑1 (MQ=255)
          cTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCAATGGCAGGctc                                                                                           <  2:799219/100‑1 (MQ=255)
          cTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCAATGGCAGGctc                                                                                           <  1:2672090/100‑1 (MQ=255)
           tGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCAATGGCAGGctca                                                                                          <  2:4312390/100‑2 (MQ=255)
           tGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCAATGGCAGGctc                                                                                           <  1:2717215/99‑1 (MQ=255)
           tGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCAGAGCCTGCCAATGGCAGGctca                                                                                          >  1:397084/1‑99 (MQ=255)
            gTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCAATGGCAGGctcag                                                                                         <  2:2275118/100‑3 (MQ=255)
            gTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCAGAGCCTGCCAATGGCAGGctcag                                                                                         <  2:397085/100‑3 (MQ=255)
            gTTCCCCTCACCCTAACCCTCGCCCCGGAGGGGCGCGCAGCCTCTCCGCGCACCTCTTTAGCCCGCGTCATCAGTCTGAGCCTCCCATTCGCAGGctct                                                                                          >  1:4219847/1‑99 (MQ=16)
             ttCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTgg                                                                                        <  2:1504354/100‑1 (MQ=255)
             ttCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTgg                                                                                        <  1:4305289/100‑1 (MQ=255)
             ttCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATGGCAGGctcagac                                                                                       <  2:266320/100‑5 (MQ=255)
              tCCCCTCACCCTACCCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCAATGGCAGGctcagac                                                                                       <  1:3065841/100‑5 (MQ=255)
               ccccTCACCCTAGCCCCCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCGGAGCCTGCCAATGGCAGGctcag                                                                                         <  1:3420458/97‑3 (MQ=37)
               ccccTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGgtg                                                                                      <  2:3570032/100‑1 (MQ=255)
               ccccTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGgtg                                                                                      >  1:3570013/1‑100 (MQ=255)
               ccccTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTGTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGgtg                                                                                      <  1:2188498/100‑1 (MQ=255)
                cccTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGctctagatc                                                                                     >  2:2848469/1‑95 (MQ=255)
                cccTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGgtgt                                                                                     >  1:594015/1‑100 (MQ=255)
                 ccTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGgtgt                                                                                     >  1:1031011/1‑99 (MQ=255)
                  cTCACCCTAACCCTCTCCCCGGAGGTGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTcc                                                                                   <  2:2125892/100‑1 (MQ=255)
                      cccTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCtttt                                                                               >  1:2969266/1‑100 (MQ=255)
                      cccTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCtttt                                                                               <  2:3449402/100‑1 (MQ=255)
                       ccTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTa                                                                              <  1:1292556/100‑1 (MQ=255)
                       ccTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTa                                                                              <  2:3055153/100‑1 (MQ=255)
                        cTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTa                                                                              <  1:1761781/99‑1 (MQ=255)
                        cTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTAc                                                                             <  1:2074984/100‑1 (MQ=255)
                        cTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTAc                                                                             <  1:4094323/100‑1 (MQ=255)
                         tAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACg                                                                            <  1:686002/100‑1 (MQ=255)
                         tAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACg                                                                            >  2:2429487/1‑100 (MQ=255)
                         tAACCCGCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACg                                                                            <  1:4090586/100‑1 (MQ=255)
                             ccTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTAc                                                                        <  2:2323443/100‑1 (MQ=255)
                              ctctCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTAcc                                                                       >  1:4257087/1‑100 (MQ=255)
                                 tcCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATg                                                                    <  2:594017/100‑1 (MQ=255)
                                 tcCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATg                                                                    >  1:1864897/1‑100 (MQ=255)
                                 tcCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATg                                                                    >  1:2854974/1‑100 (MQ=255)
                                  ccccGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGc                                                                   >  1:1950420/1‑100 (MQ=255)
                                  ccccGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGc                                                                   >  1:975039/1‑100 (MQ=255)
                                    ccGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCta                                                                 <  2:2585717/100‑1 (MQ=255)
                                    ccGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCt                                                                  <  1:3208032/99‑1 (MQ=255)
                                     cGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCtaa                                                                <  1:402472/100‑1 (MQ=255)
                                       gAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCtaata                                                              <  2:3191407/100‑1 (MQ=255)
                                        aGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTTCCATGCtaataa                                                             <  2:2893289/100‑1 (MQ=255)
                                        aGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCtaataa                                                             <  1:2936426/100‑1 (MQ=255)
                                            gCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAg                                                         >  2:3147975/1‑100 (MQ=255)
                                             cGAGGGGACTGTCCGGGCACATTTTTATATTTTGTCATCAGTCTGAGCCTGCAATTGGCAGGCTCTGGTATCCTATTACGATACCATGATaataataa                                                          >  1:4164736/1‑96 (MQ=255)
                                             cGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGc                                                        <  2:2223265/100‑1 (MQ=255)
                                             cGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGc                                                        <  1:247536/100‑1 (MQ=255)
                                              gAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGca                                                       >  1:3173322/1‑100 (MQ=255)
                                                ggggACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGcaca                                                     >  1:950813/1‑100 (MQ=255)
                                                ggggACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGcaca                                                     <  2:1257167/100‑1 (MQ=255)
                                                  ggACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACaat                                                   <  2:1039168/100‑1 (MQ=255)
                                                   gACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACaata                                                  >  1:1504598/1‑100 (MQ=255)
                                                      tGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATc                                               <  2:2936528/100‑1 (MQ=255)
                                                        tCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAg                                             <  2:1135952/100‑1 (MQ=255)
                                                         ccGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGc                                            >  2:548948/1‑100 (MQ=255)
                                                         ccGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGc                                            >  1:1980256/1‑100 (MQ=255)
                                                         ccGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCAATGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGc                                            <  2:3116636/100‑1 (MQ=255)
                                                          cGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGcc                                           <  1:3448278/100‑1 (MQ=255)
                                                          cGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGcc                                           >  1:510979/1‑100 (MQ=255)
                                                             gCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCaa                                        >  2:1907403/1‑100 (MQ=255)
                                                              cacaTTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAAc                                       >  2:3998094/1‑100 (MQ=255)
                                                                caTTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCa                                     >  2:1864307/1‑100 (MQ=255)
                                                                 aTTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCAc                                    <  2:2043547/100‑1 (MQ=255)
                                                                  tttttAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACg                                   <  2:3425869/100‑1 (MQ=255)
                                                                      tAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGt                               <  1:1184019/100‑1 (MQ=255)
                                                                         aCTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTCCCATGCTAATAATCAGCACAATAATCTGCCCAACCCTGCAGTTg                             <  1:3672703/99‑1 (MQ=255)
                                                                          cTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGAc                           >  1:3093931/1‑100 (MQ=255)
                                                                           tttGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGAcc                          <  1:45983/100‑1 (MQ=255)
                                                                              gTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGc                       <  2:4257117/100‑1 (MQ=255)
                                                                               tcatcaGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCt                      >  2:850886/1‑100 (MQ=255)
                                                                                catcaGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTc                     >  2:1350187/1‑100 (MQ=255)
                                                                                catcaGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTc                     >  2:3350004/1‑100 (MQ=255)
                                                                                 ggacaccagAGCCTGCCAATGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTcc                    >  1:1356179/9‑100 (MQ=38)
                                                                                 atcaGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTcc                    >  1:3264139/1‑100 (MQ=255)
                                                                                  gacaccagAGCCTGCCAATGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCca                   >  1:925443/8‑100 (MQ=39)
                                                                                    caccagAGCCTGCCAATGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACACTAATCAGCCCAACCACGGAGTTGACCAGCTAcagc                 >  1:2004356/6‑100 (MQ=38)
                                                                                    aGTCTGAGCCTGCCATTGGCCGGCTCTGCTTGCATTTTATGCTACTATGCTAATAATCCGCACAACAATCAGCCCAACCACGGGGTTAACCAGCtaaagc                 >  1:2289141/1‑95 (MQ=255)
                                                                                    aGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATTATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCcag                  <  2:1892992/99‑1 (MQ=255)
                                                                                      tCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCcagcag               >  2:1133831/1‑100 (MQ=255)
                                                                                       ntGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGa              >  2:4086582/2‑100 (MQ=255)
                                                                                       cagAGCCTGCCAATGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGa              >  2:1532710/3‑100 (MQ=255)
                                                                                       cagAGCCTGCCAATGGCAGGCGCAGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCcagc                 >  1:4195294/3‑97 (MQ=255)
                                                                                        tGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGAc             <  2:2154831/100‑1 (MQ=255)
                                                                                         gAGCCTGCCAATGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGAcc            >  1:165522/1‑100 (MQ=255)
                                                                                          aGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGActc           <  2:2971462/100‑3 (MQ=255)
                                                                                           gCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGAcccc          >  1:2795515/1‑100 (MQ=255)
                                                                                             cTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAg        >  1:4371499/1‑100 (MQ=255)
                                                                                              tGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGTGTTGACCAGCTCCAGCAGACCCCAgg       <  1:3041324/100‑1 (MQ=255)
                                                                                              tGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCa         >  1:2643499/1‑98 (MQ=255)
                                                                                                  aTTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGtttt   <  1:1252392/100‑1 (MQ=255)
                                                                                                   ttGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTc  <  2:1068509/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
CTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTC  >  NC_000913/4549833‑4550031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: