Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 659,207 A→G 17.9% intergenic (+57/+44) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913659,2070AG17.9% 379.5 / 74.1 180intergenic (+57/+44)tatE/lipATatABCE protein translocation system subunit/lipoate synthase
Reads supporting (aligned to +/- strand):  ref base A (81/66);  new base G (25/7);  total (106/74)
Fisher's exact test for biased strand distribution p-value = 1.76e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.51e-01

Only 100 of 187 total aligned reads displayed.

CGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAA  >  NC_000913/659111‑659306
                                                                                                |                                                                                                   
  ttGATCTTCAGGCTGAAAAGCTCTCTGATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCg                                                                                                >  2:2675264/1‑100 (MQ=255)
   tGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCgg                                                                                               <  2:1298813/100‑1 (MQ=255)
   tGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCgg                                                                                               <  1:3636541/100‑1 (MQ=255)
    gATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGGCGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCggg                                                                                              >  2:2175016/1‑100 (MQ=255)
       ctggaGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATAACTTGTTCGATACAAAAAACCTGCTGCAAAAAGCgcg                                                                                              >  1:3338350/5‑95 (MQ=255)
       cTTCATGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTTTTGAAGCGGGttt                                                                                           <  1:2539370/100‑1 (MQ=38)
       cTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTTTTGAAGCGGGttt                                                                                           <  2:2475705/100‑1 (MQ=39)
        ttCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGtttt                                                                                          >  2:4008260/1‑100 (MQ=255)
         ttacgatgcaaaGCTCTTTCATAAACAAGGAGACGCCGAGCAGGACGGTCACTCAATATCTTGTTTAATACAAAAACCCCGCTTCAAAAAGCGGGgtttt                                                                                         <  1:2956775/91‑1 (MQ=255)
           aGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTa                                                                                       >  1:3416020/1‑100 (MQ=255)
            ggCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTTTTGAAGCGGGttttttg                                                                                       <  1:2432013/99‑2 (MQ=37)
            ggCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTAt                                                                                      <  2:1766402/100‑1 (MQ=255)
             gCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATc                                                                                     <  2:161418/100‑1 (MQ=255)
              cTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTTTTGAAGCGGGttttttgtat                                                                                    <  2:1824848/100‑5 (MQ=37)
              atGAGAAGCTCGCTCATAAAGCGTGACGTGGCGAGCAGGGCGCTCCTTCATTACGTTGTTCGATACAAAAACCCCGCTCCAAAAAGCGGGGTTTTTATca                                                                                    >  1:3245188/2‑100 (MQ=255)
               tGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATcag                                                                                   <  2:3558131/100‑1 (MQ=255)
                gAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGTTACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATcag                                                                                   <  1:666242/99‑1 (MQ=255)
                      ctctctCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATg                                                                            >  2:170408/1‑100 (MQ=255)
                        ctctcATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATgta                                                                          >  2:2349874/1‑100 (MQ=255)
                        ctctcATAAAGAGTGACGTGGCGAGCAGGACGCCCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATgta                                                                          <  2:473992/100‑1 (MQ=255)
                        ctctcATAAAGAGTGACGTGGCGAGCAGAACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATgta                                                                          <  2:877335/100‑1 (MQ=255)
                          ctcATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATgtaag                                                                        <  2:1423324/100‑1 (MQ=255)
                          ctcATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATgtaag                                                                        >  2:2614129/1‑100 (MQ=255)
                           tcATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATgtaagta                                                                      <  2:4031409/100‑1 (MQ=255)
                             aTAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATgtaagtaa                                                                     >  1:2105961/1‑100 (MQ=255)
                             aTAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATgtaagtaa                                                                     >  2:550328/1‑100 (MQ=255)
                                aaGAGTGACGTGGCGAGCAGGACCTTCCCTCAATCTCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAatta                                                                  <  1:4366700/100‑1 (MQ=255)
                                 agagTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAattat                                                                 <  2:208850/100‑1 (MQ=255)
                                    gTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTAc                                                              >  2:2426263/1‑100 (MQ=255)
                                     tGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATAAAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACa                                                             <  2:37611/100‑1 (MQ=255)
                                      gACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAg                                                            >  1:2545404/1‑100 (MQ=255)
                                       aCGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAgg                                                           <  2:381371/100‑1 (MQ=255)
                                        cGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGa                                                          >  2:123945/1‑100 (MQ=255)
                                           ggCGAGCAGGACGCTCGCTCAATATCTTGTTCGATACAAGAAACCCGCTTCAAAAAGGGGGTTTTTCCTCAGACAGATGTAAGTAATTATTACAGGATTa                                                       >  1:3318054/1‑100 (MQ=255)
                                           ggCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTa                                                       >  1:1897168/1‑100 (MQ=255)
                                             cGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACt                                                     >  2:1240167/1‑100 (MQ=255)
                                             cGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACt                                                     >  2:4273227/1‑100 (MQ=255)
                                             cGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACt                                                     >  2:414022/1‑100 (MQ=255)
                                              gAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACtt                                                    <  1:550326/100‑1 (MQ=255)
                                               aGCAGGACGCTCCTTCAATATCTTGTTTGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTa                                                   <  1:159264/100‑1 (MQ=255)
                                                gCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTaa                                                  >  1:524527/1‑100 (MQ=255)
                                                 cAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAAc                                                 <  2:3416038/100‑1 (MQ=255)
                                                  aGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACt                                                >  1:4322449/1‑100 (MQ=255)
                                                   ggACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACtt                                               <  2:1961942/100‑1 (MQ=255)
                                                   ggACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACtt                                               >  2:3450881/1‑100 (MQ=255)
                                                        cTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCAt                                           >  1:4017529/1‑99 (MQ=255)
                                                         tCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATcc                                         <  1:3746946/100‑1 (MQ=255)
                                                         tCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATc                                          <  2:1353696/99‑1 (MQ=255)
                                                           ccTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCt                                       >  1:1687981/1‑100 (MQ=255)
                                                            cTCAATATCTTGTTCTATACAAAAAGGCCGCTTCAAAAAGCTGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCt                                       <  1:3937718/99‑1 (MQ=255)
                                                              cAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTc                                    >  2:1540366/1‑100 (MQ=255)
                                                               aaTATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCg                                   <  1:1384996/100‑1 (MQ=255)
                                                                atatCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGc                                  <  1:3823747/100‑1 (MQ=255)
                                                                 tatCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGcc                                 <  2:1624561/100‑1 (MQ=255)
                                                                  atCTTCTTCGATACAAAAAAAAAAAAAAAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGcc                                 <  1:1066847/99‑1 (MQ=255)
                                                                    gttGGTCGATCCAAAAAACCCGCTTCACAAAGCGGGCTTTTTATCAAACAGATGTATGGAACGACAAGAGGATCACCAAACTTCCATCCCTTTCTCCTGc                              >  1:2493544/2‑100 (MQ=255)
                                                                    cTTGTTCGTTACAAACAACCCTCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTg                               <  1:1656593/99‑1 (MQ=255)
                                                                     ttGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCa                             >  2:571473/1‑100 (MQ=255)
                                                                     ttGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGGTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCa                             >  1:4343654/1‑100 (MQ=255)
                                                                        ttCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAAt                          >  2:3685532/1‑100 (MQ=255)
                                                                         tCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATc                         >  2:667794/1‑100 (MQ=255)
                                                                          cGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCg                        <  2:3880565/100‑1 (MQ=255)
                                                                           gATACAAAAAACCCGCTTCCAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCgg                       <  1:891429/100‑1 (MQ=255)
                                                                           ctgataaaaaaCCCGCTTTTTGAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCgg                       >  1:1733585/6‑100 (MQ=25)
                                                                            tgataaaaaaCCCGCTTTTTGAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGc                      >  2:511923/5‑100 (MQ=37)
                                                                            tgataaaaaaCCCGCTTTTTGAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGc                      >  2:2298274/5‑100 (MQ=37)
                                                                            tgataaaaaaCCCGCTTTTTGAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGc                      >  1:3613187/5‑100 (MQ=37)
                                                                            tgataaaaaaCCCGCTTTGTGAAGCGGGTTTTATATCAGACAGCTGTAAGTACTTATTACAGGATTACTTACCTTACATCCCTTTCGCCTGCaaa                           >  1:804538/5‑95 (MQ=25)
                                                                             tACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCg                     >  2:2551076/1‑100 (MQ=255)
                                                                             gataaaaaaCCCGCTTTTTGAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCg                     >  2:2521908/4‑100 (MQ=37)
                                                                             gataaaaaaCCCGCTTTTTGAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCg                     >  1:3739725/4‑100 (MQ=37)
                                                                             gataaaaaaCCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCg                     <  2:939433/97‑1 (MQ=255)
                                                                              ataaaaaaCCCGCTTTTTGAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGCTTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGt                    >  2:249621/3‑100 (MQ=37)
                                                                               taaaaaaCCCGCTTTTTGAAGCGGGTTTTTTATCAGACAGATGTAAGTTCTTATTACAGGATTACTCAACTTACATCCATTTCGCTTGCAAATCGGCGTg                   >  1:597307/2‑100 (MQ=25)
                                                                               taaaaaaCCCGCTTTTTGAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTAAACTTCAATCCCTTTAGCCTGCAAATCGGCGt                    >  1:4160333/2‑99 (MQ=25)
                                                                               cAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTg                   >  2:436371/1‑100 (MQ=255)
                                                                               cAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTg                   >  2:3151417/1‑100 (MQ=255)
                                                                               cAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTg                   <  2:765940/100‑1 (MQ=255)
                                                                               cAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTg                   >  2:2255266/1‑100 (MQ=255)
                                                                                aaaaaaCCCGCTTTTTGAAGCGGGTTTTTTATCAGACAGATGTAAGTAGTTAGTACAGGATGACTTGACTTCCGTGGCTGTCGCCTGCGTAGCGGCGTgg                  >  2:3507506/1‑100 (MQ=12)
                                                                                aaaaaaCCCGCTTTTTGAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTgg                  >  2:2834332/1‑100 (MQ=38)
                                                                                aaaaaaCCCGCTTTTTGAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTgg                  >  2:3879321/1‑100 (MQ=38)
                                                                                aaaaaaCCCGCTTTTTGAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTAGCCTGCAAATCGGCGt                    >  1:4271475/1‑98 (MQ=37)
                                                                                aaaaaaCCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTgg                  <  2:3235691/100‑1 (MQ=255)
                                                                                 aaaaaCCCGCTTTTTGAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGt                 >  1:1814915/1‑100 (MQ=38)
                                                                                 aaaaaCCCGCTTTTTGAAGCGGGTTTGTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTgg                  >  2:2933082/1‑99 (MQ=38)
                                                                                  aaaaCCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTAATACAGGATTACTTAACATCCATCCCTTGCGCCTACAAATCGGCGTGGTa                >  1:967889/1‑100 (MQ=255)
                                                                                  aaaaCCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATAATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTa                >  1:1802318/1‑100 (MQ=255)
                                                                                  aaaaCCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAAATATTACAGGATTACTTAACTTCCATCACTTTCGCCTACAAATCGGCGTGGTa                >  1:2282789/1‑100 (MQ=255)
                                                                                      cccGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTaagaa            >  1:3161759/1‑100 (MQ=255)
                                                                                        cGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAagaaga          >  2:106231/1‑100 (MQ=255)
                                                                                        cGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAagaaga          <  2:3319120/100‑1 (MQ=255)
                                                                                        cGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAagaaga          >  2:1547083/1‑100 (MQ=255)
                                                                                           ttCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCg       >  2:3903724/1‑100 (MQ=255)
                                                                                            tCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTATTTAACTTCCATCCCTTTCTCCTGCAAATCGGCGTGGTAAGAACAGCg       >  1:1942215/1‑99 (MQ=255)
                                                                                            tCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCgg      >  1:3406535/1‑100 (MQ=255)
                                                                                              aaaaaGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGAc    <  2:3782474/100‑1 (MQ=255)
                                                                                              aaaaaGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGAc    >  1:4240601/1‑100 (MQ=255)
                                                                                              aaaaaGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGAc    >  1:1058643/1‑100 (MQ=255)
                                                                                               aaaaGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACa   <  1:280114/100‑1 (MQ=255)
                                                                                                |                                                                                                   
CGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAA  >  NC_000913/659111‑659306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: