BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	691,390	G→C	15.3%	A132G (GCC→GGC)	ybeX ←	putative ion transport

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	691,390	0	G	C	15.3%	606.4 / 89.3	220	A132G (GCC→GGC)	ybeX	putative ion transport
Reads supporting (aligned to +/- strand): ref base G (94/92); new base C (14/20); total (108/112)
Fisher's exact test for biased strand distribution p-value = 3.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.68e-01

Only 100 of 224 total aligned reads displayed.

ATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGG  >  NC_000913/691291‑691488

aTGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAgg                                                                                                    <  1:3130045/100‑1 (MQ=255)

 tgtgGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGc                                                                                                   <  2:1577585/100‑1 (MQ=255)

  gtgGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCt                                                                                                  <  1:3229664/100‑1 (MQ=255)

  gtgGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGAAAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCt                                                                                                  >  1:1957583/1‑100 (MQ=255)

    ggTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTc                                                                                                >  2:2754564/1‑100 (MQ=255)

    ggTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGATTAGTTTAAGGTACGACAACCGCCTGACGTAACGCGTGGTTCATGCTGAAGGCtt                                                                                                 >  1:4006492/1‑99 (MQ=255)

      tAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAg                                                                                              >  1:1944783/1‑100 (MQ=255)

         cGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGCCTTCAGCAt                                                                                           <  2:3640561/100‑1 (MQ=255)

          gCTGATAGCGAAACTCTTTCAGCATCCGGGCGACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTGTGGACATGCTGAAGCCGCCAGCAtg                                                                                          <  1:211454/100‑2 (MQ=255)

          gCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGCCGTAACACTTTGTCCATGCTGAAGCCTTCAGCAtg                                                                                          <  1:4324240/100‑2 (MQ=255)

          gCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATc                                                                                          <  2:3737413/100‑1 (MQ=255)

          gCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGCCTTCAGCAtg                                                                                          <  2:1539059/100‑2 (MQ=255)

          gCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGCCTTCAGCAtg                                                                                          <  2:3875410/100‑2 (MQ=255)

           cTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCAt                                                                                           >  1:1739699/1‑98 (MQ=255)

           cTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGCCTTCAGCAtgg                                                                                         <  2:3708453/100‑3 (MQ=255)

           cTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGCCTTCAGCAtgg                                                                                         <  2:1173889/100‑3 (MQ=255)

            tGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGCCTTCAGCAtgga                                                                                        <  2:2117228/100‑4 (MQ=255)

             gagaGCGAAACTCTATCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGCCTTCAGCAtggac                                                                                       <  1:2996660/100‑5 (MQ=255)

              agagCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTg                                                                                      <  2:3438107/100‑1 (MQ=255)

                agCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTgcg                                                                                    <  2:2247887/100‑1 (MQ=255)

                 gCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTgcgc                                                                                   <  2:853108/100‑1 (MQ=255)

                 gCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTgcgc                                                                                   >  2:1863931/1‑100 (MQ=255)

                  cGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCa                                                                                  <  2:4233397/100‑1 (MQ=255)

                  cGAAACTCTGTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCa                                                                                  <  1:2793007/100‑1 (MQ=255)

                   gAAACTCTTTCAGCATCCAGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCAt                                                                                 >  2:1794077/1‑100 (MQ=255)

                    aaaCTCTATCATCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTAGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATa                                                                                >  1:2305890/1‑100 (MQ=255)

                         ctTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACgg                                                                           >  2:2865672/1‑100 (MQ=255)

                           ttCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGgca                                                                         <  1:585711/100‑1 (MQ=255)

                           ttCAGCATCCGGTCTAAGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGgca                                                                         >  1:1277307/1‑100 (MQ=255)

                            tCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGgcag                                                                        <  2:1437764/100‑1 (MQ=255)

                            tCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGgcag                                                                        <  1:1127222/100‑1 (MQ=255)

                            tCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGgcag                                                                        <  1:1371792/100‑1 (MQ=255)

                             cAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTTAAGGCTTCAGCATCGCTGCGCATAAACGgcagc                                                                       <  1:2636746/100‑1 (MQ=255)

                              aGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGgcagca                                                                      >  1:2862668/1‑100 (MQ=255)

                              aGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGgcagca                                                                      <  2:3530380/100‑1 (MQ=255)

                                 aTCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCaa                                                                     >  1:3701906/1‑98 (MQ=255)

                                 aTCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGt                                                                   >  2:1395720/1‑100 (MQ=255)

                                 aTCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGt                                                                   <  2:13122/100‑1 (MQ=255)

                                  tCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTc                                                                  <  1:3902093/100‑1 (MQ=255)

                                  tCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTc                                                                  <  2:1781023/100‑1 (MQ=255)

                                  tCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCACTGCGCATAAACGGCAGCAAGTc                                                                  <  1:765619/100‑1 (MQ=255)

                                    cGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCtt                                                                <  2:1616178/100‑1 (MQ=255)

                                       tCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCg                                                             <  2:2191654/100‑1 (MQ=255)

                                        cTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGc                                                            >  1:853827/1‑100 (MQ=255)

                                           cgcgCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCAt                                                         >  1:912828/1‑100 (MQ=255)

                                            gcgcTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATc                                                        <  1:1454242/100‑1 (MQ=255)

                                                   ctgtCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAACCGGCAGCAAGTCTTACGCCATCAGAATcc                                                 <  1:345107/97‑1 (MQ=255)

                                                   cTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATcc                                                 >  2:906338/1‑100 (MQ=255)

                                                    tttCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCCGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATccc                                                >  2:3575642/1‑100 (MQ=255)

                                                    tttCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATccc                                                <  1:1840843/100‑1 (MQ=255)

                                                     ttCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCt                                               >  1:4160986/1‑100 (MQ=255)

                                                     ttCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCt                                               <  1:125531/100‑1 (MQ=255)

                                                      tCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCtt                                              <  2:4017350/100‑1 (MQ=255)

                                                       cAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTc                                             <  2:101867/100‑1 (MQ=255)

                                                       cAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTc                                             <  2:1081986/100‑1 (MQ=255)

                                                         ggAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCaa                                           >  1:3689821/1‑100 (MQ=255)

                                                         ggAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCaa                                           >  2:1733642/1‑100 (MQ=255)

                                                         ggAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCaa                                           >  1:4098018/1‑100 (MQ=255)

                                                          gAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAAt                                          >  2:4290336/1‑100 (MQ=255)

                                                           aaCGACTACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCTTCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAAtg                                         <  1:1041968/100‑1 (MQ=255)

                                                           aaCGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAAtg                                         <  1:497903/100‑1 (MQ=255)

                                                             cgacgaCCGCCTGACGTAACACTTTGTCCATGCTGAAGGCCTCAGCATAGCTGCGCATAAACGGCAGCAAGTCTCTCGCCCTCAGAATCCCTTCAAtgtg                                       >  1:1803055/1‑100 (MQ=255)

                                                              gACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGa                                      >  2:4160207/1‑100 (MQ=255)

                                                                cAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATc                                    >  1:2486331/1‑100 (MQ=255)

                                                                cAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATc                                    >  2:2849529/1‑100 (MQ=255)

                                                                 aaCCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCt                                   >  2:3782781/1‑100 (MQ=255)

                                                                   ccGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCttt                                 >  2:1531863/1‑100 (MQ=255)

                                                                   ccGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCttt                                 >  2:4068817/1‑100 (MQ=255)

                                                                     gCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGt                               >  2:11442/1‑100 (MQ=255)

                                                                     gCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGt                               >  2:1281145/1‑100 (MQ=255)

                                                                      ccTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTc                              <  2:3898533/100‑1 (MQ=255)

                                                                         gACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTc                           <  2:1496242/100‑1 (MQ=255)

                                                                         gACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTc                           >  2:124486/1‑100 (MQ=255)

                                                                            gTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCt                        <  1:3065966/100‑1 (MQ=255)

                                                                            gTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCt                        <  2:13367/100‑1 (MQ=255)

                                                                            gTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCt                        >  1:675012/1‑100 (MQ=255)

                                                                                cacTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATc                    >  2:3299227/1‑100 (MQ=255)

                                                                                cacTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATc                    >  1:3022145/1‑100 (MQ=255)

                                                                                cacTTTGTCCATGCTGAAGGCTTCAGCATAGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGTATCCCTTCAATGTGATCTTTGTCTTCGCTAATc                    <  1:2638823/100‑1 (MQ=255)

                                                                                  cTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCAc                  >  2:1030275/1‑100 (MQ=255)

                                                                                     tGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAACCGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCCCCgg               <  1:1522206/100‑1 (MQ=255)

                                                                                     tGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCgg               >  2:3581979/1‑100 (MQ=255)

                                                                                     cagcgaTGCTGAAGCCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCgg               >  2:3455271/6‑100 (MQ=255)

                                                                                      gTCCATGCTGAAGGCTTCATCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCggg              <  1:1615577/100‑1 (MQ=255)

                                                                                       cgatctcTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGa             <  1:801992/94‑1 (MQ=255)

                                                                                        cgaTGCTGAAGCCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGaa            >  2:3215403/3‑100 (MQ=255)

                                                                                        ccATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGaa            >  2:777742/1‑100 (MQ=255)

                                                                                         cATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGaaa           >  2:494970/1‑100 (MQ=255)

                                                                                         cATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGaaa           >  2:864863/1‑100 (MQ=255)

                                                                                           tGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACg         <  2:1815454/100‑1 (MQ=255)

                                                                                           tGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACg         >  1:2068746/1‑100 (MQ=255)

                                                                                           tGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGACTTTCGCCATCAGAATCCCATCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACg         >  1:2401305/1‑100 (MQ=255)

                                                                                            gCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGt        <  1:3883639/100‑1 (MQ=255)

                                                                                            gCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGt        >  2:3417079/1‑100 (MQ=255)

                                                                                              tGAAGCCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGa      >  2:4267997/1‑100 (MQ=255)

                                                                                               gAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCCATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAg     >  2:2453185/1‑100 (MQ=255)

                                                                                               gAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAg     >  1:1032238/1‑100 (MQ=255)

                                                                                               gAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAg     <  1:4107137/100‑1 (MQ=255)

                                                                                                 aGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTg   <  1:3104072/100‑1 (MQ=255)

                                                                                                 aGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTg   <  2:2780674/100‑1 (MQ=255)

ATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGG  >  NC_000913/691291‑691488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`