Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 83,590 G→T 18.3% intergenic (‑61/+32) leuA ← / ← leuL 2‑isopropylmalate synthase/leu operon leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091383,5900GT18.3% 571.5 / 120.1 241intergenic (‑61/+32)leuA/leuL2‑isopropylmalate synthase/leu operon leader peptide
Reads supporting (aligned to +/- strand):  ref base G (82/115);  new base T (23/21);  total (105/136)
Fisher's exact test for biased strand distribution p-value = 2.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.24e-03

Only 100 of 243 total aligned reads displayed.

GCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATA  >  NC_000913/83491‑83688
                                                                                                   |                                                                                                  
gcgcAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATg                                                                                                    >  1:2454349/1‑100 (MQ=255)
gcgcAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATg                                                                                                    >  1:575096/1‑100 (MQ=255)
     aTGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGcgcg                                                                                               <  1:287754/100‑1 (MQ=255)
      tgtgGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCgg                                                                                              <  1:821380/100‑1 (MQ=255)
      tgtgGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCgg                                                                                              <  2:4105755/100‑1 (MQ=255)
       gtgGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGGCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCggn                                                                                             <  1:836595/100‑2 (MQ=255)
        tgGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCGGGt                                                                                            <  1:3408016/100‑1 (MQ=255)
        tgGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCGGGt                                                                                            <  2:1422615/100‑1 (MQ=255)
        tgGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCGGGt                                                                                            <  1:497556/100‑1 (MQ=255)
        tgGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCGGGt                                                                                            <  2:3967669/100‑1 (MQ=255)
         ggTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCGGGtt                                                                                           <  2:3138360/100‑1 (MQ=255)
         ggTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCGGGtt                                                                                           <  2:1490205/100‑1 (MQ=255)
          gtgtCGAAAATGATGACTTGCTGGCTCAGGGTTAGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGTGCATAAAAAAACCCGTGCCATTGCGCGGGtt                                                                                           <  1:3718087/96‑1 (MQ=255)
           tATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCGGGtttt                                                                                         <  1:3320948/100‑1 (MQ=255)
           tATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGtttt                                                                                         <  2:47041/100‑1 (MQ=255)
           tATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGtttt                                                                                         <  1:1489055/100‑1 (MQ=255)
           tATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTCTAGAGCGCCTCGCTGCGGGCATAAAAAATCCCGCGCAATGGCGCGGGttt                                                                                          <  1:456658/99‑1 (MQ=255)
            aTCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCGGGttttt                                                                                        <  2:2911472/100‑1 (MQ=255)
            aTCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGttttt                                                                                        <  2:1060976/100‑1 (MQ=255)
            aTCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGttttt                                                                                        >  2:2569161/1‑100 (MQ=255)
            aTCGAAAATAATGACTTGCTGGCTAATGGTTTGGGTCCTTGTCTCTTTTTGCGCTCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGttttt                                                                                        <  1:3458833/100‑1 (MQ=255)
             tCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCGGGtttttt                                                                                       <  2:2105023/100‑1 (MQ=255)
             tCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGtttttt                                                                                       <  2:2860206/100‑1 (MQ=255)
              cGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttg                                                                                      >  2:2241289/1‑100 (MQ=255)
              cGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttg                                                                                      >  2:1351757/1‑100 (MQ=255)
              cGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttg                                                                                      >  2:1143523/1‑100 (MQ=255)
               gAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgt                                                                                     <  1:1096893/100‑1 (MQ=255)
               gAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgt                                                                                     <  2:1721854/100‑1 (MQ=255)
               gAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgt                                                                                     >  1:1420206/1‑100 (MQ=255)
                 aaaTAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgttt                                                                                   >  2:1449157/1‑100 (MQ=255)
                  aataatGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgtttg                                                                                  <  1:1013083/100‑1 (MQ=255)
                  aataatGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgtttg                                                                                  <  2:2055533/100‑1 (MQ=255)
                  aataatGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgtttg                                                                                  <  1:4319496/100‑1 (MQ=255)
                   ataatGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgtttg                                                                                  >  2:4393779/1‑99 (MQ=255)
                    taatGCCTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGTTGCGGGCATAAAAAAACCCTCGCAATGGCGCGGGTTTTTTGTTTGAc                                                                                <  1:1924842/100‑1 (MQ=255)
                    taatGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCGGGtttttttatgc                                                                                  <  2:1197333/98‑6 (MQ=37)
                    taatGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGAc                                                                                >  1:3030902/1‑100 (MQ=255)
                    taatGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGAc                                                                                >  1:1449896/1‑100 (MQ=255)
                       tGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGc                                                                             >  2:4295878/1‑100 (MQ=255)
                         aCTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGt                                                                           >  2:652074/1‑100 (MQ=255)
                         aCTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGt                                                                           <  1:243928/100‑1 (MQ=255)
                         aCTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGt                                                                           <  1:1216541/100‑1 (MQ=255)
                           ttGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGc                                                                         <  2:1934124/100‑1 (MQ=255)
                            tGCTGACTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCt                                                                        >  2:2512778/1‑100 (MQ=255)
                             gCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTg                                                                       <  2:2148685/100‑1 (MQ=255)
                             gCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTg                                                                       <  2:2618852/100‑1 (MQ=255)
                             gCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTg                                                                       <  1:4058232/100‑1 (MQ=255)
                              cTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTgg                                                                      <  2:4358081/100‑1 (MQ=255)
                                ggCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCt                                                                    >  1:228424/1‑100 (MQ=255)
                                ggCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCt                                                                    >  2:734231/1‑100 (MQ=255)
                                 gCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCtt                                                                   >  1:411184/1‑100 (MQ=255)
                                  cTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTa                                                                  >  2:3371664/1‑100 (MQ=255)
                                   tCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTaa                                                                 <  2:3226477/100‑1 (MQ=255)
                                      tGGTTTGGGTCCTTGTCTCTTTTAGAGCACCTCGCATCGGGCATATAAAAAACCGCGAAATGGCGCGCGAGTTTTGTTTGAATGCGTCCTGGCTTAATGc                                                              >  1:559027/1‑100 (MQ=255)
                                        gTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGc                                                              >  1:2659571/1‑98 (MQ=255)
                                        gTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGTTg                                                            >  2:2192763/1‑100 (MQ=255)
                                        gTTTAGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACACGCGCAATGGCGCGGTTTGTTTGCTTGACTGCGTGCTGGCTTAAt                                                                >  1:2757913/1‑96 (MQ=255)
                                           tGGGTCCTTGTCTCTTTTAGAGCGCCTTGCTTCGGGCATAAAAAATCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGAt                                                         <  1:2401610/100‑1 (MQ=255)
                                               tCCTTGTCGCTTTTGCAGCGGCTCGTTTCGGGTTTTAAAAGAGTAGCTCAATGGCGCGGGTTTTTTGTTTTAATGCGTGCTGGCTTATTGCTGGATGCCg                                                     <  1:1961196/100‑1 (MQ=255)
                                                  ttGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAGAAGCCCGCGCGATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTc                                                  <  1:1314710/100‑1 (MQ=255)
                                                      ctctTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTc                                              >  2:3307077/1‑100 (MQ=255)
                                                      ctctTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTc                                              >  1:2251451/1‑100 (MQ=255)
                                                      ctctTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTc                                              >  1:1246970/1‑100 (MQ=255)
                                                      ctctTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTc                                              <  2:3701016/100‑1 (MQ=255)
                                                       tctTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCg                                             >  2:535755/1‑100 (MQ=255)
                                                       tctTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCg                                             <  2:4141861/100‑1 (MQ=255)
                                                       tctTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCg                                             <  2:3884075/100‑1 (MQ=255)
                                                       tctTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCg                                             <  1:770934/100‑1 (MQ=255)
                                                       tctTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCg                                             <  2:513184/100‑1 (MQ=255)
                                                        tctttAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGTTGGCTTAATGCTGGATGCCGCTCACTCGt                                            >  2:3860927/3‑100 (MQ=255)
                                                          tttAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCt                                          <  2:880387/100‑1 (MQ=255)
                                                                  gcCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCgcgc                                   >  1:311050/1‑99 (MQ=255)
                                                                      cGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACCCGTCTACCGCGCAaaga                              <  1:882706/100‑1 (MQ=255)
                                                                         ttCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAaagaag                            <  2:606480/99‑1 (MQ=255)
                                                                           cGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATg                         >  2:2649255/1‑100 (MQ=255)
                                                                           agtcaaacaaaaaaCCCGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATg                         >  1:2405975/9‑100 (MQ=37)
                                                                            gggCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGc                        >  1:2887154/1‑100 (MQ=255)
                                                                            gggCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGc                        >  2:1719787/1‑100 (MQ=255)
                                                                             ggCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCg                       <  2:1251445/100‑1 (MQ=255)
                                                                             ggCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCg                       >  1:4338109/1‑100 (MQ=255)
                                                                               cATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGCTGACGCTCACTGGTCTACCGCGCAAAGAAGATGCGt                      >  1:344212/1‑99 (MQ=255)
                                                                                aTAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGttt                    <  2:2809174/100‑1 (MQ=255)
                                                                                 tAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTta                   >  1:2373828/1‑100 (MQ=255)
                                                                                   aaaaaaCCCGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCg                       >  1:4305594/1‑94 (MQ=255)
                                                                                   aaaaaaCCCGCGCCATTGCGCGGGTTGTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagt                 >  2:2682271/1‑100 (MQ=39)
                                                                                   aaaaaaCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagt                 <  2:3057377/100‑1 (MQ=255)
                                                                                   aaaaaaCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagt                 <  1:2959211/100‑1 (MQ=255)
                                                                                   aaaaaaCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagt                 >  2:4014929/1‑100 (MQ=255)
                                                                                   aaaaaaCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagt                 <  2:3452995/100‑1 (MQ=255)
                                                                                   aaaaaaCACGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagt                 >  2:2310125/1‑100 (MQ=255)
                                                                                     aaaaCCCGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtag               >  2:751703/1‑100 (MQ=255)
                                                                                     aaaaCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtag               <  2:3639774/100‑1 (MQ=255)
                                                                                      aaaCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagt              <  2:82149/100‑1 (MQ=255)
                                                                                         cccGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtagt           <  2:3695204/100‑1 (MQ=255)
                                                                                         cccGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtag            <  1:1636410/99‑1 (MQ=255)
                                                                                          ccGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtagta          <  2:3481088/100‑1 (MQ=255)
                                                                                           cgcgCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtagtag         <  2:2453174/100‑1 (MQ=255)
                                                                                               cAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCg     >  1:3264881/1‑100 (MQ=255)
                                                                                               cAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCCCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCg     <  1:1072198/100‑1 (MQ=255)
                                                                                                  tGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGTCGCGCACTCATCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATa  <  1:3745996/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
GCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATA  >  NC_000913/83491‑83688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: