Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,211,610 A→G 18.5% intergenic (+33/+70) icdC → / ← iraM pseudogene, isocitrate dehydrogenase C‑terminal gene fragment; idcC' is a 54 codon 3' gene fragment created during e14 prophage insertion/RpoS stabilzer during Mg starvation, anti‑RssB factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,211,6100AG18.5% 433.1 / 82.8 193intergenic (+33/+70)icdC/iraMpseudogene, isocitrate dehydrogenase C‑terminal gene fragment; idcC' is a 54 codon 3' gene fragment created during e14 prophage insertion/RpoS stabilzer during Mg starvation, anti‑RssB factor
Reads supporting (aligned to +/- strand):  ref base A (84/73);  new base G (21/15);  total (105/88)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 203 total aligned reads displayed.

TCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTAT  >  NC_000913/1211511‑1211709
                                                                                                   |                                                                                                   
tCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGta                                                                                                     >  2:3702517/1‑100 (MQ=255)
 cTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGtat                                                                                                    <  2:1232877/100‑1 (MQ=255)
 cTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGtat                                                                                                    >  1:1987834/1‑100 (MQ=255)
    aTGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAac                                                                                                 <  1:1455954/100‑1 (MQ=255)
     tGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAaca                                                                                                <  1:3139838/100‑1 (MQ=255)
     tGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTCGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAaca                                                                                                <  1:2319095/100‑1 (MQ=255)
      ggAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAacac                                                                                               >  2:3690007/1‑100 (MQ=255)
       gAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACg                                                                                              >  2:192529/1‑100 (MQ=255)
         aGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGcc                                                                                            <  2:2654631/100‑1 (MQ=255)
         aGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGcc                                                                                            <  1:1861456/100‑1 (MQ=255)
         aGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGcc                                                                                            <  2:1911805/100‑1 (MQ=255)
           gcgcTAAGCTGCTGAAATGTTCAGAGTTTGGTGAATCGATCATCGAAAACATGGAATCACCCCCCCTGTTAAATATTGAAACGGGCGTATAACACGcc                                                                                            <  1:1371064/98‑1 (MQ=21)
           gcgcTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGccc                                                                                           <  1:3147599/99‑1 (MQ=255)
            cgcTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTGTTATACGCCCgt                                                                                         <  2:1105159/100‑1 (MQ=25)
            cgcTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTGTTATACGCCCgt                                                                                         <  2:3650564/100‑1 (MQ=25)
            cgcTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCgt                                                                                         <  1:2063856/100‑1 (MQ=255)
            cgcTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCgt                                                                                         >  2:1473257/1‑100 (MQ=255)
              cTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCgttg                                                                                       <  1:3225264/100‑1 (MQ=255)
               tAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTGTTATACGCCCgtttc                                                                                      <  2:1769484/100‑3 (MQ=25)
               tAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCgttgt                                                                                      <  2:971195/100‑1 (MQ=255)
               tAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCgttgt                                                                                      <  2:1173975/100‑1 (MQ=255)
                 aGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTGTTATACGCCCgtttcaa                                                                                    <  2:687768/100‑5 (MQ=25)
                 aGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGttt                                                                                    <  2:2128865/100‑1 (MQ=255)
                   ctgctgAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTttta                                                                                  <  1:1754017/100‑1 (MQ=255)
                    tgctgAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTtttat                                                                                 >  2:288356/1‑100 (MQ=255)
                      ctgAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTtttattt                                                                               <  2:840675/100‑1 (MQ=255)
                        gAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTttatttat                                                                             >  1:839372/1‑100 (MQ=255)
                        gAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTttatttat                                                                             >  1:729427/1‑100 (MQ=255)
                        gAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTttatttat                                                                             >  1:3992623/1‑100 (MQ=255)
                             gTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACTGGCGTATAACACGCCCGTTGTTTTATTTAtgtg                                                                          <  1:4382410/98‑1 (MQ=255)
                              ttCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGat                                                                       >  1:4111583/1‑100 (MQ=255)
                               tCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGata                                                                      >  2:240287/1‑100 (MQ=255)
                               tCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGata                                                                      <  2:2839089/100‑1 (MQ=255)
                               tCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGATTTATTTATGTGGata                                                                      >  1:2570147/1‑100 (MQ=255)
                                 agagTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGAtatt                                                                    >  2:1498717/1‑100 (MQ=255)
                                  gagTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACCCGCCCGTTGTTTTATTTATGTGGAtatta                                                                   >  1:1222097/1‑100 (MQ=255)
                                    gTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATTTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATattatt                                                                 <  2:195906/100‑1 (MQ=255)
                                        ggTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATa                                                             >  2:580319/1‑100 (MQ=255)
                                         gTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAg                                                            >  2:2671152/1‑100 (MQ=255)
                                         gTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAg                                                            >  1:1672486/1‑100 (MQ=255)
                                          tGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGc                                                           <  1:4191396/100‑1 (MQ=255)
                                          tGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGc                                                           <  1:544929/100‑1 (MQ=255)
                                           gAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCa                                                          >  2:4209204/1‑100 (MQ=255)
                                           gAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCa                                                          >  2:2178164/1‑100 (MQ=255)
                                           gAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCa                                                          <  2:3431810/100‑1 (MQ=255)
                                             aGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCata                                                        <  2:3470392/100‑1 (MQ=255)
                                               cGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCatatn                                                      <  2:1929566/100‑2 (MQ=255)
                                               cGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATc                                                      <  2:3599863/100‑1 (MQ=255)
                                                gATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCg                                                     >  2:595949/1‑100 (MQ=255)
                                                  tcatcGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAg                                                   <  2:2554694/100‑1 (MQ=255)
                                                  tcatcGAAAACATGTAATCTCTCCATGTGTTAAATACTGAATCGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAg                                                   <  1:2426197/100‑1 (MQ=255)
                                                   catcGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGc                                                  <  1:3335926/100‑1 (MQ=255)
                                                    atcGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGc                                                  <  1:1312459/99‑1 (MQ=255)
                                                    atcGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCa                                                 <  2:2284285/100‑1 (MQ=255)
                                                    atcGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCa                                                 <  2:541024/100‑1 (MQ=255)
                                                       gAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGCGCatat                                              >  1:1125220/1‑100 (MQ=255)
                                                        aaaaCATGTAATCTCTCCATGGGTTGAATATTTAAACGGGCGTATAACACGACAGTTGTATTATTTAGGTGGATATTATTAATAGCATATCGaagatct                                              >  1:4284136/1‑93 (MQ=255)
                                                         aaaCATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATAttt                                            <  1:288355/100‑1 (MQ=255)
                                                            cATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTtata                                         <  1:1051106/100‑1 (MQ=255)
                                                            cATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTtata                                         <  1:3683927/100‑1 (MQ=255)
                                                              tGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATg                                       <  2:79726/100‑1 (MQ=255)
                                                              tGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATg                                       >  2:3056233/1‑100 (MQ=255)
                                                               gTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGa                                      >  1:3003656/1‑100 (MQ=255)
                                                                 aaTCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAg                                    >  2:464620/1‑100 (MQ=255)
                                                                  aTCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGc                                   <  1:2604688/100‑1 (MQ=255)
                                                                      ctcCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCAt                               <  1:958635/100‑1 (MQ=255)
                                                                       tcCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCAtt                              >  2:1376257/1‑100 (MQ=255)
                                                                         cATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTAc                            <  1:3807598/100‑1 (MQ=255)
                                                                         cATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTAc                            <  2:4380080/100‑1 (MQ=255)
                                                                              gtTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAg                       <  1:1792275/100‑1 (MQ=255)
                                                                              gtTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAg                       >  1:2459081/1‑100 (MQ=255)
                                                                              gtTAAATATTGAAACAGGCGTATAACACGCTCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAg                       <  2:4334003/100‑1 (MQ=255)
                                                                               ttAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGc                      <  2:3272879/100‑1 (MQ=255)
                                                                                tAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGcc                     >  2:2038504/1‑100 (MQ=255)
                                                                                 aaaTATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGccc                    >  2:654791/1‑100 (MQ=255)
                                                                                  aaTATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCa                   >  2:1980099/1‑100 (MQ=255)
                                                                                   ataaaacaaCGGGCGTGTTATACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCat                  >  2:2376903/8‑100 (MQ=37)
                                                                                    taaaacaaCGGGCGTGTTATACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCata                 >  2:3403144/7‑100 (MQ=38)
                                                                                    taaaacaaCGGGCGTGTTATACGCCCGTTGTTTCATTTATGTGGATATTATTAATAGCATATCGAGCATCGTTATATGAAGCCCATTACTTGAGCCCata                 >  1:2640472/7‑100 (MQ=25)
                                                                                      ttGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATg               <  1:1671814/100‑1 (MQ=255)
                                                                                      ttGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATg               >  2:3805569/1‑100 (MQ=255)
                                                                                      aaacaaCGGGCGTGTTATACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATg               >  2:1352574/5‑100 (MQ=38)
                                                                                      aaacaaCGGGCGTGTTATACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATg               >  2:3872861/5‑100 (MQ=38)
                                                                                      aaacaaCGGGCGTGTTATACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATg               >  2:2223837/5‑100 (MQ=38)
                                                                                        gAAACGTGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATggg             <  1:2811961/100‑1 (MQ=255)
                                                                                        gAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATggg             <  1:439767/100‑1 (MQ=255)
                                                                                        acaaCGGGCGTGTTATACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATggg             >  2:2755130/3‑100 (MQ=255)
                                                                                         caaCGGGCGTGTTATACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATgg              >  2:1046116/2‑98 (MQ=255)
                                                                                         caaCGGGCGTGTTATACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGc            >  2:496622/2‑100 (MQ=255)
                                                                                         caaCGGGCGTGTTATACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGc            >  1:2540625/2‑100 (MQ=255)
                                                                                         caaCGGGCGTGTTATACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGc            >  2:2722154/2‑100 (MQ=255)
                                                                                         aaaCGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGc            >  2:918176/1‑100 (MQ=255)
                                                                                           aCGGGCGTGTTATACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCat          >  2:1279236/1‑100 (MQ=255)
                                                                                            cGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCata         >  1:3020349/1‑100 (MQ=255)
                                                                                            cGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCata         <  1:3811226/100‑1 (MQ=255)
                                                                                               gCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATAttt      >  1:2054907/1‑100 (MQ=255)
                                                                                                cGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATAtttt     >  1:2760090/1‑100 (MQ=255)
                                                                                                 gTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATAttttt    >  1:4169965/1‑100 (MQ=255)
                                                                                                   ataACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTtat  <  2:3003211/100‑1 (MQ=255)
                                                                                                   ataACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTtat  <  2:4022147/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
TCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTAT  >  NC_000913/1211511‑1211709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑