BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,226,832	T→G	16.6%	F78C (TTT→TGT)	ycgJ →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,226,832	0	T	G	16.6%	421.5 / 81.3	180	F78C (TTT→TGT)	ycgJ	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base T (86/63); new base G (16/14); total (102/78)
Fisher's exact test for biased strand distribution p-value = 6.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.30e-01

Only 100 of 182 total aligned reads displayed.

GAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAAT  >  NC_000913/1226734‑1226929

 aaCTCAGATGGCATTTCCCCAGGGTTAACAAGGAAATATCTCGGCTAAAAAGCCGTTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTa                                                                                                 <  2:3486525/100‑1 (MQ=255)

 aaCTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTa                                                                                                 >  1:2416658/1‑100 (MQ=255)

 aaCTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTa                                                                                                 <  2:2416669/100‑1 (MQ=255)

  aCTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTAc                                                                                                >  2:640381/1‑100 (MQ=255)

  aCTCAGATGGCATTTCCCCAGAGTTAACAAGGAAAAAAAAAAAAAAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTAc                                                                                                <  1:3916027/100‑1 (MQ=255)

   cTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTGCAATCATTACAAGGCTACGATCCCAGCGAATTTACa                                                                                               >  1:159137/1‑100 (MQ=255)

   cTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACa                                                                                               <  1:2206862/100‑1 (MQ=255)

    tCAGATGGCATTTCCCCAGAGTTAACAAGAAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACAt                                                                                              <  1:1564013/100‑1 (MQ=255)

     cAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACAtt                                                                                             >  2:2598870/1‑100 (MQ=255)

     cAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACAtt                                                                                             >  2:1683192/1‑100 (MQ=255)

     aaGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCACTGAAAACTTACAATCATTATAAGGCTACGATCCCAGGGAATTTAc                                                                                                >  1:4241933/2‑97 (MQ=255)

        aTGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGc                                                                                          <  1:3365149/100‑1 (MQ=255)

         tGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCt                                                                                         <  1:2848960/100‑1 (MQ=255)

          ggCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTa                                                                                        >  1:1934193/1‑100 (MQ=255)

           ggaTTTCCCCAGAGTTAAAAAGGAAATAGCTCGGCGAAAAAGCAGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATGTAAATTCGCtgg                                                                                       <  1:1348471/98‑3 (MQ=255)

           gCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATGTAAATTCGCtgg                                                                                       <  2:1472761/100‑3 (MQ=255)

           gCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATGTAAATTCGCtgg                                                                                       <  2:2044927/100‑3 (MQ=255)

           gCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATGTAAATTCGCtgg                                                                                       <  1:2877691/100‑3 (MQ=255)

            cATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAAc                                                                                      >  1:3006502/1‑100 (MQ=255)

            cATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATGTAAATTCGCtggg                                                                                      <  2:1432184/100‑4 (MQ=255)

            cATTTCCCCAGAGGTAACAAGGAAATATCTCGGCGAAACAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAAc                                                                                      >  1:2713649/1‑100 (MQ=255)

             aTTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATGTAAATTCGCtggg                                                                                      <  2:3935354/99‑4 (MQ=255)

              tttCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACgg                                                                                    <  1:1552847/100‑1 (MQ=255)

              tttCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACgg                                                                                    <  2:1159428/100‑1 (MQ=255)

              tttCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATGTAAATTCGCtgggat                                                                                    <  1:3709490/100‑6 (MQ=255)

                tCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGgtg                                                                                  >  1:1002384/1‑100 (MQ=255)

                tCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGgtg                                                                                  <  2:4188659/100‑1 (MQ=255)

                 ccccAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTAAATTCGCTAACGgtgt                                                                                 >  1:2657778/1‑100 (MQ=255)

                  cgcAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTa                                                                                >  2:2985470/3‑100 (MQ=255)

                  cccAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTa                                                                                >  1:177277/1‑100 (MQ=255)

                   ccAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTAt                                                                               >  1:3413483/1‑100 (MQ=255)

                      gagTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTTCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTa                                                                                <  2:2932023/96‑1 (MQ=255)

                        gTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGc                                                                          >  2:472174/1‑100 (MQ=255)

                         ttAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCg                                                                         <  2:2469258/100‑1 (MQ=255)

                          tAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGa                                                                        <  1:1227549/100‑1 (MQ=255)

                           aaCAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGAt                                                                       <  2:2639584/100‑1 (MQ=255)

                            aCAAGGAAATATCTCGGCGAAAAAGCCGCTGAAGACTTACAATCATTACAAGGCTACGATCCCAGCGAATGTACATTCGCTAACGGTGTATTTTGCGATg                                                                      >  1:2652356/1‑100 (MQ=255)

                              aaGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGtt                                                                    >  1:708091/1‑100 (MQ=255)

                               aGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTa                                                                   <  1:3886992/100‑1 (MQ=255)

                                 gAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCCGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTaaa                                                                 >  2:3458522/1‑100 (MQ=255)

                                  aaaTATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAg                                                                >  1:2126542/1‑100 (MQ=255)

                                     tatCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGaa                                                              <  2:75894/99‑1 (MQ=255)

                                      atCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGaaaa                                                            >  2:2578695/1‑100 (MQ=255)

                                       tctcGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGaaaaa                                                           >  2:1338330/1‑100 (MQ=255)

                                        ctcGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATTCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAAt                                                          <  2:1061947/100‑1 (MQ=255)

                                        ctcGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAAt                                                          >  1:424760/1‑100 (MQ=255)

                                        ctcGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAAt                                                          >  1:3063958/1‑100 (MQ=255)

                                        ctcGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAAt                                                          >  2:3271325/1‑100 (MQ=255)

                                         tcGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAAtt                                                         >  1:2046908/1‑100 (MQ=255)

                                          cGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTa                                                        >  1:4014737/1‑100 (MQ=255)

                                          cGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATATACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTa                                                        <  1:3265264/100‑1 (MQ=255)

                                            gCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATg                                                      >  2:485046/1‑100 (MQ=255)

                                                  aaGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGtga                                                <  2:3134103/100‑1 (MQ=255)

                                                    gCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGtgatg                                              >  1:2746427/1‑100 (MQ=255)

                                                    gCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGtgatg                                              >  2:221532/1‑100 (MQ=255)

                                                    gCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGtgatg                                              >  2:1766720/1‑100 (MQ=255)

                                                      cGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTgatgat                                            <  2:4245210/100‑1 (MQ=255)

                                                        cTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCg                                          <  1:2948202/100‑1 (MQ=255)

                                                            aaaCTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTAt                                      <  2:1227728/100‑1 (MQ=255)

                                                             aaCTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTAtt                                     <  2:639008/100‑1 (MQ=255)

                                                             aaCTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTAt                                      >  1:3140100/1‑99 (MQ=255)

                                                              aCTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTAttt                                    >  1:3398278/1‑100 (MQ=255)

                                                               cTTGCAATCATTAAAAGGCTACGATCCCAGCGAATTTACATTTGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTAttt                                    <  1:2271213/99‑1 (MQ=255)

                                                                ttAGAATCAATACAAGGCAACGGTTACAGAAGAGGTATATTCGCTAACGGTTTATTTTGAAAAAAAAAAAAAAAATTATGTCGTGATGATCGTTATTTTg                                  <  1:2976603/100‑1 (MQ=255)

                                                                 tACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTgg                                 >  2:600550/1‑100 (MQ=255)

                                                                  aCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGgt                                <  2:4045023/100‑1 (MQ=255)

                                                                  aCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGgt                                >  1:903217/1‑100 (MQ=255)

                                                                   cAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGgtg                               <  2:3196224/100‑1 (MQ=255)

                                                                    aaTCATTACAAGGCTACGATCACAGCGAATTTACATTCGTTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGgtgt                              <  2:239284/100‑1 (MQ=255)

                                                                      tCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGtgtg                             >  1:1246959/1‑99 (MQ=255)

                                                                      tCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTgg                            >  1:3852491/1‑100 (MQ=255)

                                                                      tCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTgg                            >  2:2950134/1‑100 (MQ=255)

                                                                      tCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTgg                            <  2:3236661/100‑1 (MQ=255)

                                                                       cATTACAAGGCTACGATCTCATCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATTATCGTTATTTTGGTGTGGa                           <  2:1999954/100‑1 (MQ=255)

                                                                        aTTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGAt                          <  2:4262951/100‑1 (MQ=255)

                                                                         ttACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGAt                          <  1:3247829/99‑1 (MQ=255)

                                                                          tACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATgg                        <  1:1381315/100‑1 (MQ=255)

                                                                              aGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTAAGTCGTGATGATCGTTATTTTGGTGCGGAAGGTAAg                    >  1:347961/1‑100 (MQ=255)

                                                                               ggCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGc                   >  2:3800691/1‑100 (MQ=255)

                                                                                gCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCg                  <  1:4212452/100‑1 (MQ=255)

                                                                                 cTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCgg                 >  2:256374/1‑100 (MQ=255)

                                                                                 cTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCgg                 <  2:2320849/100‑1 (MQ=255)

                                                                                 cTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCgg                 <  2:3732325/100‑1 (MQ=255)

                                                                                  tACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGa                >  1:4363940/1‑100 (MQ=255)

                                                                                   aCGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAg               <  2:2746442/100‑1 (MQ=255)

                                                                                    cGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAACAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGt              >  2:1427996/1‑100 (MQ=255)

                                                                                    cGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGt              >  1:2764576/1‑100 (MQ=255)

                                                                                     gATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTg             <  1:285663/100‑1 (MQ=255)

                                                                                     gATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTg             >  2:1122169/1‑100 (MQ=255)

                                                                                      ccgttaGCGAATGTAAATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTgg            >  2:1583928/6‑100 (MQ=255)

                                                                                        gttaGCGAATGTAAATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAACATTATGTCGTGATGATCGTTCTTTTGGTGTGGATGGTAAGCGGAGGGGaa          >  2:4273124/4‑100 (MQ=255)

                                                                                         ttaGCGAATGTAAATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGaaa         >  2:934021/3‑100 (MQ=255)

                                                                                         ccAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGaaa         >  2:3625851/1‑100 (MQ=255)

                                                                                         ccAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGaa          <  1:609312/99‑1 (MQ=255)

                                                                                          cAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGCCGTGATGATCGTTATTTTGGTGTGGATGCTAAGCGGAGTGGaaa         >  1:364348/1‑99 (MQ=255)

                                                                                             cGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAaatc     <  2:96177/100‑1 (MQ=255)

                                                                                              gAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAaatca    <  2:846147/100‑1 (MQ=255)

                                                                                                aTTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTTTATGGTAAGCGGAGTGGAAAaatcaat  <  1:3037048/100‑1 (MQ=255)

                                                                                                aTTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAaatcaat  <  1:2552751/100‑1 (MQ=255)

                                                                                                 tttACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAaatcaa   <  1:3852869/98‑1 (MQ=255)

GAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAAT  >  NC_000913/1226734‑1226929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`