Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,322,398 C→G 16.7% V183V (GTG→GTC)  trpE ← component I of anthranilate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,322,3980CG16.7% 464.3 / 85.3 188V183V (GTG→GTC) trpEcomponent I of anthranilate synthase
Reads supporting (aligned to +/- strand):  ref base C (85/70);  new base G (24/7);  total (111/77)
Fisher's exact test for biased strand distribution p-value = 2.70e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.85e-01

Only 100 of 191 total aligned reads displayed.

ACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAG  >  NC_000913/1322299‑1322497
                                                                                                   |                                                                                                   
aCGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATc                                                                                                     <  1:596669/100‑1 (MQ=255)
 cGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATc                                                                                                     >  2:2402325/1‑99 (MQ=255)
 cGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCa                                                                                                    <  2:3959395/100‑1 (MQ=255)
 cGTAGTTCGTTCAGGAGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCa                                                                                                    <  2:2851943/100‑1 (MQ=255)
    aGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCa                                                                                                 >  2:1240352/1‑100 (MQ=255)
      ttcgttcAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATc                                                                                               <  2:1820180/100‑1 (MQ=255)
      ttcgttcAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATc                                                                                               >  2:3177523/1‑100 (MQ=255)
        cgttcAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAATCAGGCTGGCCTTAATACGGTTGCTTTTTTTCTGATGGTCAAGCACCATCAg                                                                                             <  1:844749/100‑1 (MQ=255)
        cgttcAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTGATTGACCATCAg                                                                                             <  2:2297107/100‑1 (MQ=255)
        cgttcAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTGATTGACCATCAg                                                                                             <  2:3727539/100‑1 (MQ=255)
        cgttcAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAg                                                                                             >  1:3353635/1‑100 (MQ=255)
         gttcGGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGc                                                                                            >  1:1465289/1‑100 (MQ=255)
         gttcAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGc                                                                                            >  1:1592541/1‑100 (MQ=255)
         gttcAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGc                                                                                            >  1:792456/1‑100 (MQ=255)
          ttcAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTGATTGACCATCAgaa                                                                                           <  1:1115269/100‑3 (MQ=255)
           tcAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTGATTGACCATCAgaaa                                                                                          <  1:3650129/100‑4 (MQ=255)
              ggCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTc                                                                                       <  1:1364227/100‑1 (MQ=255)
              ggCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTc                                                                                       >  2:3308978/1‑100 (MQ=255)
                cGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTGATTGACCATCAgaaaaaaag                                                                                     <  1:4091385/100‑9 (MQ=38)
                cGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAg                                                                                     >  2:928398/1‑100 (MQ=255)
                   gCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCga                                                                                  <  1:1805202/100‑1 (MQ=255)
                   gCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCga                                                                                  >  2:666348/1‑100 (MQ=255)
                    cAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCg                                                                                   >  1:946190/1‑98 (MQ=255)
                      gTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGAt                                                                               <  2:607060/100‑1 (MQ=255)
                       tGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATa                                                                              <  1:4062719/100‑1 (MQ=255)
                       tGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATa                                                                              <  1:3056118/100‑1 (MQ=255)
                            cGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAAc                                                                         <  1:2763166/100‑1 (MQ=255)
                             gttgttTTTCTTCTTCATTCGGATCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAAc                                                                         <  2:2568268/99‑1 (MQ=255)
                               tgttTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGa                                                                      >  1:2029871/1‑100 (MQ=255)
                                gttTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGaa                                                                     >  2:1688582/1‑100 (MQ=255)
                                 tttttCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGaaa                                                                    <  2:1604693/100‑1 (MQ=255)
                                   tttCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATc                                                                  >  2:1123996/1‑100 (MQ=255)
                                   nttcttcttcATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATc                                                                  >  2:3099953/2‑100 (MQ=255)
                                      cttcttcATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAgg                                                               >  2:547837/1‑100 (MQ=255)
                                       ttcttcATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAggg                                                              <  2:3600068/100‑1 (MQ=255)
                                        tcttcATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTTATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGc                                                             >  1:2624975/1‑100 (MQ=255)
                                        tcttcATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGc                                                             >  1:3437830/1‑100 (MQ=255)
                                         cgtcATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCa                                                            >  2:1913799/3‑100 (MQ=255)
                                           tcATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGt                                                          >  2:3582293/1‑100 (MQ=255)
                                             aTTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTa                                                        >  2:3145054/1‑100 (MQ=255)
                                             aTTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTa                                                        >  1:3139707/1‑100 (MQ=255)
                                                 ggAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTAtttt                                                    >  1:1879970/1‑100 (MQ=255)
                                                   aGCAAACAGGCTGGCCTTAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTcc                                                  <  1:2009129/100‑1 (MQ=255)
                                                   aGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCCGGGCCGATATTTTc                                                   >  2:4118286/1‑99 (MQ=255)
                                                   aGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTcc                                                  >  2:4001113/1‑100 (MQ=255)
                                                    gCAAACAGGCTGGCCTGAATACGTGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTAAGGGAGATAAAAACAGAAATCAGGGCAGTTATTTTc                                                   <  1:1450093/98‑1 (MQ=255)
                                                     cAAACAGGCTGGCCTTAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGc                                                <  2:4329044/100‑1 (MQ=255)
                                                     cAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGc                                                <  2:4189531/100‑1 (MQ=255)
                                                     cAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGc                                                <  1:2999913/100‑1 (MQ=255)
                                                       aaCAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTg                                              >  2:2716915/1‑100 (MQ=255)
                                                         cAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGAc                                            <  1:4341122/100‑1 (MQ=255)
                                                           ggCTGGCCTGAATACGGGTGCTTTTTTTTTTTTTTTTTATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACa                                           >  1:4122161/1‑99 (MQ=255)
                                                           ggCTGGCCTGAATACGGGTGCTTTTTTTTTTTTTGTTTATCACCATCAGCGTTTCAGCGAGATAAATACAGAAATCAGGGCAGTTATTTTCCGCTGACAg                                          >  1:402401/1‑100 (MQ=255)
                                                           ggCTGGCCTGAATACGGGTGCTTTTTTTTTTATTGTCTATCACCATCAGCGTTTCACCGAGATAAAAACAGAATTCAGGGCAGTTATTTTCCGCTGACAg                                          >  1:1514507/1‑100 (MQ=255)
                                                           ggCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCCTCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGAc                                            <  2:1586039/98‑1 (MQ=255)
                                                            gccagCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCCGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGt                                         >  2:335526/5‑100 (MQ=255)
                                                            gCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGAc                                            <  1:3566257/97‑1 (MQ=255)
                                                             cgggCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGtt                                        >  2:2990889/3‑100 (MQ=255)
                                                               ggCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGc                                      >  1:232679/1‑100 (MQ=255)
                                                                gCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAAAAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCg                                     >  2:706119/1‑100 (MQ=255)
                                                                  cTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGt                                   >  1:660759/1‑100 (MQ=255)
                                                                      aTACGGGTGCTTTTTTTTTTTTTTTTTATCACCATAAGCGTTTCAGCGAGATTAAATCAGATATAAGGGCAGTTTTTTTCCACTTACATTTTCGGTAAAt                               >  1:3176714/1‑100 (MQ=255)
                                                                       tACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATc                              >  2:2864954/1‑100 (MQ=255)
                                                                            gTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCaa                         <  2:954254/100‑1 (MQ=255)
                                                                            gTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCaa                         <  1:971298/100‑1 (MQ=255)
                                                                             tGCTTTTTTTCTGATAGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCaaa                        <  2:1176010/100‑1 (MQ=255)
                                                                              gctgaaacgcTGATGGTGATTGACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAAt                       >  2:2202813/10‑100 (MQ=37)
                                                                               cTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGCGATAAAAACAGAAATCAGCGCAGTTATATTCCGCTCACAGTTGCGGTCAATCTTCAAATc                      >  1:3078681/1‑100 (MQ=255)
                                                                               cTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATc                      >  2:456430/1‑100 (MQ=255)
                                                                                tttttttCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAAttc                     >  1:2429331/1‑98 (MQ=255)
                                                                                tttttttCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATcc                     >  2:2087032/1‑100 (MQ=255)
                                                                                tttttttCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATcc                     >  1:2915969/1‑100 (MQ=255)
                                                                                 ttttttCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATccc                    <  2:1677569/100‑1 (MQ=255)
                                                                                  tttttCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCg                   <  1:4197643/100‑1 (MQ=255)
                                                                                    acgcTGATGGTGATTGACCATCAGCGTTTCAGCGAGCTAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCAGcc                 >  1:2252503/4‑100 (MQ=255)
                                                                                     ttCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCca                >  2:2616257/1‑100 (MQ=255)
                                                                                     cgcTGATGGTGATTGACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCTCca                >  1:2876444/3‑100 (MQ=255)
                                                                                     cgcTGATGGTGATTGACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGcc                 >  1:132572/3‑99 (MQ=255)
                                                                                     cgcTGATGGTGATTGACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCca                >  2:3407708/3‑100 (MQ=255)
                                                                                     cgcTGATGGTGATTGACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCca                >  2:3523595/3‑100 (MQ=255)
                                                                                     cgcTGATGGTGATTGACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGATGACAGTTGCGGTAAATCTTCAAATCCCGCca                >  1:1945407/3‑100 (MQ=255)
                                                                                      gcTGATGGTGATTGACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCcac               >  2:2585797/2‑100 (MQ=255)
                                                                                      gcTGATGGTGATTGACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCcac               >  1:1112344/2‑100 (MQ=255)
                                                                                      gcTGATGGTGATTGACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCcac               >  2:3709192/2‑100 (MQ=255)
                                                                                      gcTGATGGTGATTGACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATATTCAAATCCCGCcac               >  2:4341747/2‑100 (MQ=255)
                                                                                       cTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCcaca              <  1:666346/100‑1 (MQ=255)
                                                                                       cTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCcaca              <  2:1520969/100‑1 (MQ=255)
                                                                                       cTGATGGACAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCGGACAGTTGCGGTAGATCTTCAAATCCAGCcac               <  1:239940/99‑1 (MQ=255)
                                                                                         gATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAg            >  1:3389592/1‑100 (MQ=255)
                                                                                          aTGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAgg           <  2:3422415/100‑1 (MQ=255)
                                                                                          aTGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAgg           <  2:1879976/100‑1 (MQ=255)
                                                                                           tGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGt          <  2:3437848/100‑1 (MQ=255)
                                                                                           tGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGt          >  1:925401/1‑100 (MQ=255)
                                                                                           tGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGt          >  2:174950/1‑100 (MQ=255)
                                                                                             gTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGTGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCa        <  2:800676/100‑1 (MQ=255)
                                                                                              tCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCAt       <  1:716803/100‑1 (MQ=255)
                                                                                               cAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAACTCTTCAAATCCCGCCACAAGGTCATa      <  2:2675533/100‑1 (MQ=255)
                                                                                               cAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATa      <  2:2029879/100‑1 (MQ=255)
                                                                                                aaTCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATaa     <  1:3166231/100‑1 (MQ=255)
                                                                                                aaTCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATaa     <  2:2816407/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
ACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAG  >  NC_000913/1322299‑1322497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑