Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,344,036 G→C 15.6% T104S (ACT→AGT)  yciT ← global regulator of transcription; DeoR family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,344,0360GC15.6% 441.2 / 64.7 160T104S (ACT→AGT) yciTglobal regulator of transcription; DeoR family
Reads supporting (aligned to +/- strand):  ref base G (79/56);  new base C (14/11);  total (93/67)
Fisher's exact test for biased strand distribution p-value = 8.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 166 total aligned reads displayed.

CACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCG  >  NC_000913/1343938‑1344135
                                                                                                  |                                                                                                   
cACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGt                                                                                                    >  2:3569137/1‑100 (MQ=255)
cACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGt                                                                                                    >  1:2857400/1‑100 (MQ=255)
  ccGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTcc                                                                                                  <  1:128286/100‑1 (MQ=255)
   cGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCg                                                                                                 <  2:52307/100‑1 (MQ=255)
   cGAGCAAAATAACTTCACAAAGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTcc                                                                                                  >  1:18071/1‑99 (MQ=255)
    gAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCgg                                                                                                >  2:922346/1‑100 (MQ=255)
    gAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCgg                                                                                                <  1:2843845/100‑1 (MQ=255)
       cAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCg                                                                                             <  2:2867675/100‑1 (MQ=255)
        aaaaTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGAGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGa                                                                                            >  2:2169180/1‑100 (MQ=255)
          aaTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCGGACTCTGGGCGAgc                                                                                          <  1:360271/100‑2 (MQ=255)
           aTAACTTCACAAGGCGCGTCTTTGAGCAAATGTGCGATGAAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTCCTCGCCCGGACTCTGGGCGAgca                                                                                         <  1:3084272/100‑3 (MQ=255)
            tAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCGGACTCTGGGCGAGCAg                                                                                        <  1:2859718/100‑1 (MQ=255)
            tAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAg                                                                                        >  2:3443113/1‑100 (MQ=255)
             aaCTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTACTCGCCCGGACTCTGGGCGAGCAga                                                                                       <  1:779541/100‑2 (MQ=38)
              aCTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAggg                                                                                      <  1:223914/100‑1 (MQ=255)
               cTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGc                                                                                     <  2:3619411/100‑1 (MQ=255)
               cTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGc                                                                                     >  2:2231197/1‑100 (MQ=255)
                  cacaAGGCGCGTCTTTCAGCAACTGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCAtt                                                                                  >  1:2560048/1‑100 (MQ=255)
                  cacaAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCAtt                                                                                  >  2:2246747/1‑100 (MQ=255)
                    caAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgc                                                                                <  2:764338/100‑1 (MQ=255)
                    caAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgc                                                                                <  2:1489779/100‑1 (MQ=255)
                    caAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgc                                                                                >  2:1369041/1‑100 (MQ=255)
                       ggCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgctgc                                                                             >  1:1058132/1‑100 (MQ=255)
                       ggCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgctgc                                                                             >  1:2228091/1‑100 (MQ=255)
                                ttCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGTCCTTGCTGCTGCCAtttt                                                                    >  1:2620512/1‑100 (MQ=255)
                                ttCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCAtttt                                                                    >  1:974906/1‑100 (MQ=255)
                                ttCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGGGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCCGCCAtttt                                                                    >  1:2613598/1‑100 (MQ=255)
                                 tCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTc                                                                   <  2:1326464/100‑1 (MQ=255)
                                  cAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCg                                                                  <  2:4087523/100‑1 (MQ=255)
                                   ngCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGa                                                                 >  1:1381844/2‑100 (MQ=255)
                                   aGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGa                                                                 >  1:1031343/1‑100 (MQ=255)
                                    gCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGAt                                                                >  2:246153/1‑100 (MQ=255)
                                    gCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGAt                                                                >  1:2771525/1‑100 (MQ=255)
                                    gCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGAt                                                                <  2:360504/100‑1 (MQ=255)
                                    gCAAATGCGCGAGGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGAt                                                                <  2:284541/100‑1 (MQ=255)
                                       aaTGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATaaa                                                             <  1:2367362/100‑1 (MQ=255)
                                        aTGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAg                                                            <  1:3964776/100‑1 (MQ=255)
                                         tGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGa                                                           <  2:679817/100‑1 (MQ=255)
                                          gcgcGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGAt                                                          >  2:1934844/1‑100 (MQ=255)
                                          gcgcGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGAt                                                          >  1:4125314/1‑100 (MQ=255)
                                            gcgATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGTGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATgg                                                        >  2:3094976/1‑100 (MQ=255)
                                            gcgATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATgg                                                        <  1:1914632/100‑1 (MQ=255)
                                            gcgATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATgg                                                        <  1:3965875/100‑1 (MQ=255)
                                                tGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCGCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTc                                                    <  1:764730/100‑1 (MQ=255)
                                                   aGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGcc                                                 >  2:779716/1‑100 (MQ=255)
                                                    gctgctGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCACAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGccc                                                >  2:117873/1‑100 (MQ=255)
                                                      tgctgACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCgg                                              <  2:2649871/100‑1 (MQ=255)
                                                       gctgACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTcagatcgg                                             >  1:353043/1‑93 (MQ=255)
                                                       gctgACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGt                                             >  1:53040/1‑100 (MQ=255)
                                                       gctgACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGAGAGAAGGGCATTGCTGCTGCCATTTTCGATCAAGATGGTTTCGCCCGGt                                             >  1:1389953/1‑100 (MQ=255)
                                                        ctgACCGTGATGATAGTGACATTTTTCTTCGGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGtt                                            <  1:3025765/100‑1 (MQ=255)
                                                         tgACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTg                                           >  1:3818479/1‑100 (MQ=255)
                                                          gACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTg                                           >  1:2579453/1‑99 (MQ=255)
                                                            ccGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAAc                                        >  2:1714285/1‑100 (MQ=255)
                                                            ccGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAAc                                        >  2:3244977/1‑100 (MQ=255)
                                                             cGTGATTATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAAcc                                       <  2:2085783/100‑1 (MQ=255)
                                                             cGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGTCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAAcc                                       <  1:2819230/100‑1 (MQ=255)
                                                             cGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAAcc                                       >  1:37005/1‑100 (MQ=255)
                                                             cGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCGGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAAcc                                       <  1:3990894/100‑1 (MQ=255)
                                                                 atgatAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCg                                   <  2:597457/100‑1 (MQ=255)
                                                                  tgatAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGa                                  >  1:441563/1‑100 (MQ=255)
                                                                  tgatAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGa                                  >  2:1622268/1‑100 (MQ=255)
                                                                   gatAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGAc                                 >  2:2362551/1‑100 (MQ=255)
                                                                    atAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGAcg                                >  2:1753595/1‑100 (MQ=255)
                                                                     tAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGAcgc                               <  1:3439819/100‑1 (MQ=255)
                                                                       gTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCgg                             >  1:1525154/1‑100 (MQ=255)
                                                                        tGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGc                            <  2:4252221/100‑1 (MQ=255)
                                                                         gACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTGGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCa                           >  1:1426779/1‑100 (MQ=255)
                                                                                ttcttcTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCgg                    <  2:3656994/100‑1 (MQ=255)
                                                                                 ttccctacTCGCCCGGACTCTGGACGAGTAGGGCGTTGCTGCTGCCATTTTCGCAAAAGATGGTGCCGCCCGGTTGAACCAGCGCCGCGGCAAACTc                      >  1:4164589/8‑97 (MQ=255)
                                                                                 tcttctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGc                   <  2:1064821/100‑1 (MQ=255)
                                                                                 tcttctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGc                   <  2:3778874/100‑1 (MQ=255)
                                                                                  cttctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCg                  <  2:3544540/100‑1 (MQ=255)
                                                                                   ccctacTCGCCCGGACTCTGGGCGAGCAGGGCATTGCTGCTGCCATTTTCGATAAAGATAGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGa                 >  2:3625463/6‑100 (MQ=37)
                                                                                   ccctacTCGCACGGACTCTGGCCGAGCAGGGCATTGCTGCTGCCATATTCGATACAGCTGGTTTCGCCCGGCTGAACCAGCGACCCGGCAAAcgcg                     >  1:3579425/6‑96 (MQ=255)
                                                                                    cctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAg                >  2:1022195/5‑100 (MQ=255)
                                                                                    cctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAg                >  1:985370/5‑100 (MQ=255)
                                                                                    cctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAg                >  2:4166124/5‑100 (MQ=255)
                                                                                    cctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAg                >  1:2007387/5‑100 (MQ=255)
                                                                                    cctacTCGCCCGGACTCTGGGCGAGCAGGGCATTGCTGCTGCCATTATCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCgg                    >  1:887927/5‑96 (MQ=37)
                                                                                     ctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGt               >  2:3847640/1‑100 (MQ=255)
                                                                                       gCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTc             >  1:2138541/1‑100 (MQ=255)
                                                                                       gCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTc             >  2:1919471/1‑100 (MQ=255)
                                                                                       acTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTc             >  1:178105/2‑100 (MQ=255)
                                                                                        cTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCa            >  1:3102554/1‑100 (MQ=255)
                                                                                        cTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCa            >  1:3204822/1‑100 (MQ=255)
                                                                                          cGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACg          >  2:2787719/1‑100 (MQ=255)
                                                                                          cGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTCGAACCAGCGACGCGGCAAACTCGGCGAGTTCACg          >  1:2319058/1‑100 (MQ=255)
                                                                                           gCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGc         <  2:2641970/100‑1 (MQ=255)
                                                                                           gCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGc         <  2:2403410/100‑1 (MQ=255)
                                                                                            cccAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCt        >  1:1428581/1‑100 (MQ=255)
                                                                                            cccAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCt        >  1:2745309/1‑100 (MQ=255)
                                                                                            cccAGAGTCCGGGCGAGTAGGGCATTGATGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGc         >  1:2109492/1‑99 (MQ=255)
                                                                                             ccAGAGTCCGGGCGAGTAGGGCATTGCTGCCGCCATTTTCGATAAAGCTGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACg          <  1:3310169/97‑1 (MQ=255)
                                                                                              cAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTc      >  1:3784490/1‑100 (MQ=255)
                                                                                               agagTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCa     >  1:533406/1‑100 (MQ=255)
                                                                                                gagTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAg    <  2:1219271/100‑1 (MQ=255)
                                                                                                gagTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAg    >  2:3751062/1‑100 (MQ=255)
                                                                                                  gTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGGCGCGGCAAACTCGGCGAGTTCACGCTTCAg    <  1:3881358/98‑1 (MQ=255)
                                                                                                  gTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCg  <  2:3503607/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
CACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCG  >  NC_000913/1343938‑1344135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑