BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	4,215,179	Δ4 bp	coding (900‑903/930 nt)	metA →	homoserine O‑transsuccinylase

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	4215179	4215182	4	101 [0]	[0] 103	metA	homoserine O‑transsuccinylase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4215178	0 (0.000)	100 (1.050)	73/196	0.1	100%	coding (899/930 nt)	metA	homoserine O‑transsuccinylase
*	?	NC_000913	4215183 =	0 (0.000)	100 (1.050)	73/196	0.1	100%	coding (904/930 nt)	metA	homoserine O‑transsuccinylase

Only 100 of 103 total aligned reads displayed.

CGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCC  >  NC_000913/4215085‑4215282

gatccgcaaaatacaccgcgagcgagctggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgacg                                                                                                    >  2:1357742‑M2/99‑100 (MQ=255)

 atccgcaaaatacaccgcgagcgagctggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCgg                                                                                                   >  2:1926888‑M2/98‑100 (MQ=255)

  tccgcaaaatacaccgcgagcgagctggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCgg                                                                                                   <  2:70050‑M2/3‑1 (MQ=255)

   ccgcaaaatccaccgcgagcgagctggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGca                                                                                                 >  2:1777752‑M2/96‑100 (MQ=255)

   ccgcaaaatacaccgcgagcgagctggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGca                                                                                                 <  1:1085074‑M2/5‑1 (MQ=255)

   ccgcaaaatacaccgcgagcgagctggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGca                                                                                                 >  1:483761‑M2/96‑100 (MQ=255)

   ccgcaaaatacaccgcgagcgagctggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGca                                                                                                 >  2:2345886‑M2/96‑100 (MQ=255)

   ccgcaaaatacaccgcgagcgagctggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGca                                                                                                 >  2:2338946‑M2/96‑100 (MQ=255)

     gcaaaatacaccgcgagcgagctggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGcaca                                                                                               >  2:1419454‑M2/94‑100 (MQ=255)

     gcaaaatacaccgcgagcgagctggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGcaca                                                                                               >  2:445990‑M2/94‑100 (MQ=255)

     gcaaaatacaccgcgagcgagctggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGcaca                                                                                               >  2:2288052‑M2/94‑100 (MQ=255)

     gcaaaatacaccgcgagcgagctggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGcaca                                                                                               >  1:1729363‑M2/94‑100 (MQ=255)

       aaaatcaaccgagagcgagctggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATg                                                                                             <  1:445990‑M2/9‑1 (MQ=255)

       aaaatacaccgcgagcgagctggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATg                                                                                             <  1:1841300‑M2/9‑1 (MQ=255)

       aaaatacaccgcgagcgagctggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATg                                                                                             <  2:2034715‑M2/9‑1 (MQ=255)

        aaatacaccgcgagcgagctggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGa                                                                                            >  1:554810‑M2/91‑100 (MQ=255)

          atacaccgcgagcgagctggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAAt                                                                                          <  2:372269‑M2/12‑1 (MQ=255)

          atacaccgcgagcgagctggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAAt                                                                                          >  1:2098210‑M2/89‑100 (MQ=255)

           tacaccgcgagcgagctggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATc                                                                                         <  1:121233‑M2/13‑1 (MQ=255)

            acaccgcgagcgagctggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATcc                                                                                        <  1:243905‑M2/14‑1 (MQ=255)

             caccgcgagcgagctggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCa                                                                                       >  2:1000373‑M2/86‑100 (MQ=255)

               ccgcgagcgagctggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAAc                                                                                     >  1:1027674‑M2/84‑100 (MQ=255)

                  cgagcgagctggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCt                                                                                  >  1:1115206‑M2/81‑100 (MQ=255)

                    agcgagctggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTgg                                                                                <  2:309858‑M2/22‑1 (MQ=255)

                    agcgagctggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTgg                                                                                <  2:578782‑M2/22‑1 (MQ=255)

                      cgagctggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGAt                                                                              <  1:906725‑M2/24‑1 (MQ=255)

                      cgagctggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGAt                                                                              >  1:1325070‑M2/77‑100 (MQ=255)

                       gagctggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGAtt                                                                             <  2:1297595‑M2/25‑1 (MQ=255)

                        agctggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTa                                                                            >  2:1977412‑M2/75‑100 (MQ=255)

                          ctggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAAt                                                                          >  2:135829‑M2/73‑100 (MQ=255)

                          ctggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAAt                                                                          >  1:2310194‑M2/73‑100 (MQ=255)

                           tggcgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAAtc                                                                         >  1:1914714‑M2/72‑100 (MQ=255)

                              cgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAAtcttc                                                                      <  1:1700939‑M2/32‑1 (MQ=255)

                              cgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAAtcttc                                                                      >  2:254752‑M2/69‑100 (MQ=255)

                              cgtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAAtcttc                                                                      <  2:790677‑M2/32‑1 (MQ=255)

                               gtagtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAAtcttct                                                                     <  2:82244‑M2/33‑1 (MQ=255)

                                 agtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCtgt                                                                   <  2:1861874‑M2/35‑1 (MQ=255)

                                 agtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCtgt                                                                   >  1:1560075‑M2/66‑100 (MQ=255)

                                 agtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCtgt                                                                   <  2:2312732‑M2/35‑1 (MQ=255)

                                  gtcacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCtgtg                                                                  >  1:1085986‑M2/65‑100 (MQ=255)

                                    cacggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGAt                                                                >  2:26517‑M2/63‑100 (MQ=255)

                                     acggtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGAt                                                                <  2:1560076‑M2/38‑1 (MQ=255)

                                        gtaatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTc                                                            <  2:2310192‑M2/42‑1 (MQ=255)

                                         taatttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCg                                                           <  2:1920535‑M2/43‑1 (MQ=255)

                                           atttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGAt                                                         >  2:2017574‑M2/56‑100 (MQ=255)

                                            tttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATc                                                        >  2:2248315‑M2/55‑100 (MQ=255)

                                             ttactgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCg                                                       >  1:225966‑M2/54‑100 (MQ=255)

                                               actgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGtt                                                     >  1:1127647‑M2/52‑100 (MQ=255)

                                                ctgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTa                                                    >  1:924951‑M2/51‑100 (MQ=255)

                                                ctgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTa                                                    >  2:688677‑M2/51‑100 (MQ=255)

                                                ctgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTa                                                    <  1:114910‑M2/50‑1 (MQ=255)

                                                ctgtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTa                                                    <  1:688676‑M2/50‑1 (MQ=255)

                                                  gtttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAg                                                  >  2:1553307‑M2/49‑100 (MQ=255)

                                                   tttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGc                                                 <  1:604397‑M2/53‑1 (MQ=255)

                                                   tttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGc                                                 <  1:1290553‑M2/53‑1 (MQ=255)

                                                   tttaccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGc                                                 <  1:2248317‑M2/53‑1 (MQ=255)

                                                     taccaactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGa                                               >  1:1303637‑M2/46‑100 (MQ=255)

                                                       ccaactggctcaactattacgtttaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGAtt                                             >  2:1529511‑M2/44‑100 (MQ=255)

                                                       ccaactggctcaactattacgtctaccagatcacgccatacgaCGACACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGAtt                                             >  1:358783‑M2/44‑100 (MQ=255)

                                                        caactggctcaactcttacgtctaccagatcacgccatacgaCGGCGCATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTc                                            >  1:385166‑M2/43‑100 (MQ=255)

                                                        caactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTAATAGTCGATCGTTAAGCGATTc                                            <  1:1777751‑M2/58‑1 (MQ=255)

                                                         aactggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCa                                           >  1:1027623‑M2/42‑100 (MQ=255)

                                                         aactggctcaactattacgtctaccagatcacgccatacgaCGACACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCa                                           <  2:358783‑M2/59‑1 (MQ=255)

                                                          actggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAg                                          >  1:2070693‑M2/41‑100 (MQ=255)

                                                          actggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAg                                          <  1:2288054‑M2/60‑1 (MQ=255)

                                                           ctggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGc                                         <  2:1720939‑M2/61‑1 (MQ=255)

                                                           ctggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGc                                         <  1:254752‑M2/61‑1 (MQ=255)

                                                             ggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCAc                                       >  1:1376340‑M2/38‑100 (MQ=255)

                                                             ggctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCAc                                       >  2:449706‑M2/38‑100 (MQ=255)

                                                              gctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCAcc                                      <  1:2338949‑M2/64‑1 (MQ=255)

                                                               ctcaactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCt                                     >  1:1858726‑M2/36‑100 (MQ=255)

                                                                 caactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTa                                   >  2:458313‑M2/34‑100 (MQ=255)

                                                                  aactattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTAc                                  <  1:1357741‑M2/68‑1 (MQ=255)

                                                                    ctattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCt                                >  2:1654978‑M2/31‑100 (MQ=255)

                                                                     tattacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTc                               <  1:1926889‑M2/71‑1 (MQ=255)

                                                                      attacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCa                              <  2:1027676‑M2/72‑1 (MQ=255)

                                                                       ttacgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAg                             <  2:1376341‑M2/73‑1 (MQ=255)

                                                                         acgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGc                           >  1:383659‑M2/26‑100 (MQ=255)

                                                                         acgtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGc                           <  2:2114834‑M2/75‑1 (MQ=255)

                                                                           gtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCAc                         >  1:1934913‑M2/24‑100 (MQ=255)

                                                                           gtctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCAc                         >  1:847374‑M2/24‑100 (MQ=255)

                                                                            tctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCAcc                        >  2:172408‑M2/23‑100 (MQ=255)

                                                                             ctaccagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCt                       >  2:518768‑M2/22‑100 (MQ=255)

                                                                                 cagatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCgg                   >  2:1651651‑M2/18‑100 (MQ=255)

                                                                                  agatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCggg                  >  2:1917371‑M2/17‑100 (MQ=255)

                                                                                  agatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCggg                  >  2:1917139‑M2/17‑100 (MQ=255)

                                                                                   gatcacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGt                 <  1:2017576‑M2/85‑1 (MQ=255)

                                                                                      cacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTacctcaggcacccgaaggtgcc              <  1:2345889‑M2/88‑11 (MQ=255)

                                                                                      cacgccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGcc              >  1:140661‑M2/13‑100 (MQ=255)

                                                                                         gccatacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCttt           >  1:1606712‑M2/10‑100 (MQ=255)

                                                                                           catacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCttttt         <  1:2019357‑M2/93‑1 (MQ=255)

                                                                                           catacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCttttt         <  1:2019195‑M2/93‑1 (MQ=255)

                                                                                            atacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCtttttt        <  1:1419453‑M2/94‑1 (MQ=255)

                                                                                             tacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTa       <  1:1529510‑M2/95‑1 (MQ=255)

                                                                                             tacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTa       <  2:554811‑M2/95‑1 (MQ=255)

                                                                                             tacgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTa       <  2:1303638‑M2/95‑1 (MQ=255)

                                                                                               cgaCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTAtt     <  2:1115209‑M2/97‑1 (MQ=255)

                                                                                                 aCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTc   <  1:1553306‑M2/99‑1 (MQ=255)

                                                                                                  cGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTcc  <  2:483761/100‑1 (MQ=255)

                                                                                                  cGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTcc  >  2:780509/1‑100 (MQ=255)

CGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCC  >  NC_000913/4215085‑4215282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`