Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,078,996	G→T	intergenic (+558/‑301)	yiiD → / → yiiE	GNAT family putative N‑acetyltransferase/CopG family putative transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,078,996	0	G	T	100.0%	297.8 / NA	81	intergenic (+558/‑301)	yiiD/yiiE	GNAT family putative N‑acetyltransferase/CopG family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (43/38);  total (43/38)

TTTAGAAAAACAACTTTCCCGAACTGTTACCAACAAAATTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAAGATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGAATATAATAT  >  NC_000913/4078899‑4079091                                                                                                  |                                                                                                tttAGAAAAACAACTTTCCCGAACTGTTACCAACAAAATTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAatat                                                                                               <  2:1074550/100‑1 (MQ=255)  ttAGAAAAACAACTTTCCCGAACTGTTACCAACAAAATTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATAtt                                                                                              >  2:766357/1‑100 (MQ=255)  ttAGAAAAACAACTTTCCCGAACTGTTACCAACAAAATTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATAtt                                                                                              <  1:400477/100‑1 (MQ=255)   tAGAAAAACAACTTTCCCGAACTGTTACCAACAAAATTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTa                                                                                             >  2:1301733/1‑100 (MQ=255)    aGAAAAACAACTTTCCCGAACTGTTACCAACAAAATTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTaa                                                                                            >  2:2357171/1‑100 (MQ=255)     gAAAAACAACTTTCCCGAACTGTTACCAACAAAATTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAAt                                                                                           >  2:880550/1‑100 (MQ=255)      aaaaaCAACTTTCCCGAACTGTTACCAACAAAATTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAAtt                                                                                          <  1:1077601/100‑1 (MQ=255)      aaaaaCAACTTTCCCGAACTGTTACCAACAAAATTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAAtt                                                                                          <  1:1115531/100‑1 (MQ=255)       aaaaCAACTTTCCCGAACTGTTACCAACAAAATTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAAttt                                                                                         >  2:215078/1‑100 (MQ=255)                ttCCCGAACTGTTACCAACAAAATTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTaaa                                                                                >  2:857183/1‑100 (MQ=255)                 tCCCGAACTGTTACCAACAAAATTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAAt                                                                               >  1:971098/1‑100 (MQ=255)                   ccGAACTGTTACCAACAAAATTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATcc                                                                             >  2:1276378/1‑100 (MQ=255)                   ccGAACTGTTACCAACAAAATTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATcc                                                                             >  2:1436051/1‑100 (MQ=255)                     gAACTGTTACCAACAAAATTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTg                                                                           <  1:1984839/100‑1 (MQ=255)                      aaCTGTTACCAACAAAATTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTgg                                                                          <  2:1187047/100‑1 (MQ=255)                      aaCTGTTACCAACAAAATTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTgg                                                                          <  2:975647/100‑1 (MQ=255)                         tGTTACCAACAAAATTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGc                                                                       >  1:28095/1‑100 (MQ=255)                         tGTTACCAACAAAATTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGc                                                                       <  2:81921/100‑1 (MQ=255)                         tGTTACCAACAAAATTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGc                                                                       <  2:1192137/100‑1 (MQ=255)                         tGTTACCAACAAAATTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGc                                                                       >  1:736713/1‑100 (MQ=255)                          gTTACCAACAAAATTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCt                                                                      >  2:985940/1‑100 (MQ=255)                           ttACCAACAAAATTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTg                                                                     <  2:28095/100‑1 (MQ=255)                           ttACCAACAAAATTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTg                                                                     <  2:971100/100‑1 (MQ=255)                            tACCAACAAAATTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGt                                                                    >  1:293707/1‑100 (MQ=255)                             aCCAACAAAATTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGtt                                                                   >  2:1665757/1‑100 (MQ=255)                             aCCAACAAAATTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGtt                                                                   <  1:634037/100‑1 (MQ=255)                               caacaaAATTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAg                                                                 >  1:1453601/1‑100 (MQ=255)                                aacaaAATTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAgg                                                                >  1:2171337/1‑100 (MQ=255)                                aacaaAATTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAgg                                                                <  2:2358215/100‑1 (MQ=255)                                  caaAATTATAACGATGAGCAAGTAAAGAGCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGa                                                               <  2:1227236/99‑1 (MQ=255)                                    aaaTTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGc                                                            <  2:1322944/100‑1 (MQ=255)                                    aaaTTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGc                                                            <  1:985938/100‑1 (MQ=255)                                       ttATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGa                                                         <  1:1067839/100‑1 (MQ=255)                                       ttATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGa                                                         <  1:280192/100‑1 (MQ=255)                                        tataACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGAc                                                        >  1:1670621/1‑100 (MQ=255)                                            aCGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATACTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGttt                                                    >  1:933439/1‑100 (MQ=255)                                             cGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGtttt                                                   <  2:2019338/100‑1 (MQ=255)                                             cGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGtttt                                                   >  2:1012875/1‑100 (MQ=255)                                             agATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGtttt                                                   >  1:1208364/2‑100 (MQ=255)                                               aTGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTc                                                 <  2:1121/100‑1 (MQ=255)                                                  aGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGTTGCTGTTAGGATGCTGACGTTTTTCGGc                                              >  2:148480/1‑100 (MQ=255)                                                  aGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGc                                              >  1:1037114/1‑100 (MQ=255)                                                  aGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGc                                              <  2:89641/100‑1 (MQ=255)                                                  aGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGc                                              <  1:2357174/100‑1 (MQ=255)                                                   gCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCt                                             <  1:1665756/100‑1 (MQ=255)                                                    cAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGTCTa                                            >  1:1492032/1‑100 (MQ=255)                                                      aGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTaca                                          >  1:1662763/1‑100 (MQ=255)                                                           aGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCAtt                                     >  1:244649/1‑100 (MQ=255)                                                           aGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCAt                                      <  2:1431530/99‑1 (MQ=255)                                                                ggTTGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGAt                                >  1:1187392/1‑100 (MQ=255)                                                                  ttGGTGGCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTa                              <  1:1455315/100‑1 (MQ=255)                                                                   tggtggCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTAt                             >  2:480790/1‑100 (MQ=255)                                                                    ggtggCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATg                            <  1:1970168/100‑1 (MQ=255)                                                                     gtggCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGc                           <  1:779832/100‑1 (MQ=255)                                                                      tggCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCa                          <  2:614857/100‑1 (MQ=255)                                                                      tggCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCa                          <  2:569165/100‑1 (MQ=255)                                                                      tggCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCa                          <  2:1208365/100‑1 (MQ=255)                                                                       ggCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCaa                         >  1:1579565/1‑100 (MQ=255)                                                                       ggCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCaa                         >  2:2112416/1‑100 (MQ=255)                                                                        gCAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCaaa                        >  2:2332854/1‑100 (MQ=255)                                                                         cAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCaaat                       <  2:1174958/100‑1 (MQ=255)                                                                         cAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCaaat                       >  1:115936/1‑100 (MQ=255)                                                                         cAGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCaaat                       <  2:810321/100‑1 (MQ=255)                                                                          aGTACTGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCaaata                      <  2:509888/100‑1 (MQ=255)                                                                             aCTGACCTATCTGTGATTAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACTTTTTTCGGCTACACCATTCAGATTATGCaaataaa                    <  1:1385121/99‑1 (MQ=255)                                                                               tGACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAatat                 >  1:332865/1‑100 (MQ=255)                                                                                gACCGGTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGCATACAACATTAAGCTTATGCAaataaata                  >  1:439740/1‑98 (MQ=255)                                                                                    ggTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTg            <  2:333730/100‑1 (MQ=255)                                                                                    ggTCTGTGATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTg            <  2:1341578/100‑1 (MQ=255)                                                                                    ccgatctgatgaAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTg            <  2:2327512/94‑1 (MQ=255)                                                                                         gtgATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGaatat       >  1:1406190/1‑100 (MQ=255)                                                                                         gtgATGAAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGaatat       >  1:2091455/1‑100 (MQ=255)                                                                                          tgatgaAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGaatata      <  2:754133/100‑1 (MQ=255)                                                                                           gatgaAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGagatcgg     >  1:2327514/1‑94 (MQ=255)                                                                                           gatgaAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGaatataa     <  2:1670622/100‑1 (MQ=255)                                                                                           gatgaAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGaatataa     >  1:1141071/1‑100 (MQ=255)                                                                                           gatgaAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGaatataa     >  2:2373630/1‑100 (MQ=255)                                                                                           gatgaAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGaatataa     >  1:556257/1‑100 (MQ=255)                                                                                             tgaAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGaatataata   >  1:1869905/1‑100 (MQ=255)                                                                                              gaAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGaatataatat  >  1:598610/1‑100 (MQ=255)                                                                                              gaAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGaatataatat  >  1:728907/1‑100 (MQ=255)                                                                                              gaAATATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGaatataatat  >  1:597703/1‑100 (MQ=255)                                                                                                  |                                                                                                TTTAGAAAAACAACTTTCCCGAACTGTTACCAACAAAATTATAACGATGAGCAAGTAAAGACCGGTTGGTGGCAGTACTGACCGGTCTGTGATGAAAGATTAATTTCACCTTAAATCCTGGTGCTGTTAGGATGCTGACGTTTTTCGGCTACACCATTCAGATTATGCAAATAAATATCTCTGAATATAATAT  >  NC_000913/4078899‑4079091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑