Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,756,529	A→T	T278S (ACC→TCC)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,529	0	A	T	98.8%	283.3 / NA	80	T278S (ACC→TCC)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (43/36);  minor base G (1/0);  total (44/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGT  >  NC_000913/1756432‑1756623                                                                                                   |                                                                                               cGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTcc                                                                                              <  2:2275241/100‑1 (MQ=255)     cTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGa                                                                                          <  2:2056295/100‑1 (MQ=255)     cTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGa                                                                                          <  2:451376/100‑1 (MQ=255)         gtgtAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCt                                                                                      >  1:104376/1‑100 (MQ=255)         gtgtAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCt                                                                                      >  2:2031873/1‑100 (MQ=255)          tgtAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTg                                                                                     >  2:244574/1‑100 (MQ=255)          tgtAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTg                                                                                     >  2:1179647/1‑100 (MQ=255)          tgtAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTg                                                                                     >  2:704325/1‑100 (MQ=255)          tgtAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTg                                                                                     <  2:1239861/100‑1 (MQ=255)          tgtAGAAATCCCGGGAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTAGCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTg                                                                                     <  1:2347593/100‑1 (MQ=255)             aGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGAt                                                                                  >  2:361246/1‑100 (MQ=255)             aGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGAt                                                                                  >  2:983374/1‑100 (MQ=255)             aGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGAt                                                                                  >  2:1488431/1‑100 (MQ=255)              gAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGAtt                                                                                 >  2:312528/1‑100 (MQ=255)              gAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGAtt                                                                                 >  1:1908993/1‑100 (MQ=255)              gAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGAtt                                                                                 >  2:167036/1‑100 (MQ=255)               aaaTCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTc                                                                                >  2:2210541/1‑100 (MQ=255)                  tCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCAt                                                                             <  1:792752/100‑1 (MQ=255)                   cccGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATg                                                                            >  1:303477/1‑100 (MQ=255)                     cGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGAt                                                                          <  2:1495707/100‑1 (MQ=255)                     cGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGAt                                                                          <  1:2350007/100‑1 (MQ=255)                      ggTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATc                                                                         >  2:147228/1‑100 (MQ=255)                      ggTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATc                                                                         >  1:40908/1‑100 (MQ=255)                       gTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCa                                                                        <  2:862311/100‑1 (MQ=255)                         agaagaAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCaaa                                                                      <  1:1050362/100‑1 (MQ=255)                            agaagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAAc                                                                   >  1:2262323/1‑100 (MQ=255)                            agaagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAAc                                                                   <  2:40908/100‑1 (MQ=255)                             gaagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTTTCCGGTCCCGGAAAAGTC‑TTAACCCTTCGGCCCCGGTGGTGGGTTCCATGATCAAAAAcc                                                                  >  1:1681153/1‑100 (MQ=255)                                gTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCAc                                                               >  2:879048/1‑100 (MQ=255)                                gTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCAc                                                               >  2:2223558/1‑100 (MQ=255)                                  tATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGc                                                             <  1:869156/100‑1 (MQ=255)                                  tATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGc                                                             >  1:870644/1‑100 (MQ=255)                                     cTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCg                                                          <  1:879047/100‑1 (MQ=255)                                     cTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCg                                                          >  2:2017001/1‑100 (MQ=255)                                     cTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCg                                                          >  2:1386723/1‑100 (MQ=255)                                       tCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGAc                                                        <  1:324993/100‑1 (MQ=255)                                       tCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGAc                                                        >  2:1326621/1‑100 (MQ=255)                                        cGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACt                                                       >  1:1746391/1‑100 (MQ=255)                                        cGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACt                                                       >  2:1435306/1‑100 (MQ=255)                                         gCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTc                                                      >  2:757624/1‑100 (MQ=255)                                          cccAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTcg                                                     <  2:285304/100‑1 (MQ=255)                                          cccAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTcg                                                     >  2:734236/1‑100 (MQ=255)                                          cccAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGAAAAGTCGTTATCACTGCGTCCCATATGCTAGATTCCATGATCAAAAACCCACGCCCGACTcg                                                     >  1:872623/1‑100 (MQ=255)                                           ccAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTCAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGAc                                                        <  2:600566/96‑1 (MQ=255)                                              gaagaTGATGATCGAGAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCa                                                 <  1:1189774/100‑1 (MQ=255)                                              gaagaTGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCa                                                 >  1:395778/1‑100 (MQ=255)                                                  atgatgatCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAg                                             <  2:1746392/100‑1 (MQ=255)                                                   tgatgatCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGc                                            >  1:832662/1‑100 (MQ=255)                                                   tgatgatCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGc                                            >  2:1170249/1‑100 (MQ=255)                                                   tgatgatCGAAAAATGTATCC‑GTGAACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCAACGCCCGACTCGCGCAGAAGc                                            >  2:208008/1‑100 (MQ=255)                                                    gatgatCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGcc                                           <  1:2210543/100‑1 (MQ=255)                                                    gatgatCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGcc                                           <  2:1656067/100‑1 (MQ=255)                                                      tgatCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCgg                                         <  2:122890/100‑1 (MQ=255)                                                       gatCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGt                                        >  2:1250694/1‑100 (MQ=255)                                                        atCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTg                                       >  1:5969/1‑100 (MQ=255)                                                         tCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGa                                      <  1:70182/100‑1 (MQ=255)                                                          cGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGAc                                     <  1:147229/100‑1 (MQ=255)                                                          cGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGAc                                     >  1:987631/1‑100 (MQ=255)                                                             aaaaTGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGtt                                  <  2:402308/100‑1 (MQ=255)                                                              aaaTGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTg                                 <  2:395778/100‑1 (MQ=255)                                                               aaTGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGc                                >  2:688809/1‑100 (MQ=255)                                                               aaTGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCACGACTCGCGCAGAAGCCGGTGACGTTGc                                >  2:1077067/1‑100 (MQ=255)                                                                aTGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCa                               <  2:1790453/100‑1 (MQ=255)                                                                  gTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCaaa                             >  1:592787/1‑100 (MQ=255)                                                                  gTATCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCaaa                             <  1:2008524/100‑1 (MQ=255)                                                                     tCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGc                          <  1:312528/100‑1 (MQ=255)                                                                     tCC‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGc                          <  1:1326620/100‑1 (MQ=255)                                                                       c‑GTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCa                        >  2:2146418/1‑100 (MQ=255)                                                                           gCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATcc                     <  1:361246/100‑1 (MQ=255)                                                                           gCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATcc                     <  1:2017003/100‑1 (MQ=255)                                                                            cACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCt                    >  1:1659734/1‑100 (MQ=255)                                                                              cGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCg                  >  1:1607228/1‑100 (MQ=255)                                                                                  aaGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACgg              <  1:1353973/100‑1 (MQ=255)                                                                                    gTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTa            >  2:2189847/1‑100 (MQ=255)                                                                                    gTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTa            >  2:666465/1‑100 (MQ=255)                                                                                       gTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTg         <  2:2177071/100‑1 (MQ=255)                                                                                        ttATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGa        <  1:2189849/100‑1 (MQ=255)                                                                                         tATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGAc       >  1:1794250/1‑100 (MQ=255)                                                                                         tATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGAc       <  2:832663/100‑1 (MQ=255)                                                                                              cTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGt  <  2:1607229/100‑1 (MQ=255)                                                                                                   |                                                                                               CGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCC‑GTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGT  >  NC_000913/1756432‑1756623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑