| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,750,720 | C→T | intergenic (‑12/‑111) | grpE ← / → nadK | heat shock protein/NAD kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,750,720 | 0 | C | T | 93.6% | 250.9 / 6.2 | 78 | intergenic (‑12/‑111) | grpE/nadK | heat shock protein/NAD kinase |
| Reads supporting (aligned to +/- strand): ref base C (3/2); new base T (33/40); total (36/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
CTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACA > NC_000913/2750622‑2750816 | cTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCcgcg < 2:254229/100‑1 (MQ=255) cgatcTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCgtgt < 2:1509916/96‑1 (MQ=255) gatcTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGtt < 2:205752/97‑1 (MQ=255) cAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGtt > 2:1434852/1‑100 (MQ=255) aCTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGtttt < 2:2133192/100‑1 (MQ=255) tGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGtttttt > 1:2343167/1‑100 (MQ=255) tGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGtttttt < 2:356542/100‑1 (MQ=255) gCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGttttttt < 1:522449/100‑1 (MQ=255) gCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGttagatc > 1:205752/1‑95 (MQ=255) tcTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCTGTGCTTACACCTCAGGTGTTTTCTGTTCTTTACTACTCATGAATTTTTCCGTGttttttt < 2:649041/98‑1 (MQ=255) ccTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGttttttt > 1:2384470/1‑99 (MQ=255) ccTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTc > 1:1288081/1‑100 (MQ=255) cTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTc > 2:2987/1‑99 (MQ=255) cTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCg > 1:1508263/1‑100 (MQ=255) cTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCg > 1:1508244/1‑100 (MQ=255) ctctTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCa > 2:1555695/1‑100 (MQ=255) ctctTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCa < 1:2215107/100‑1 (MQ=255) tctTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCAt > 1:1817132/1‑100 (MQ=255) ttCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCAtc < 2:668111/99‑1 (MQ=255) tCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCAtctc < 2:2343164/100‑1 (MQ=255) cgatcTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCg < 2:992966/96‑1 (MQ=255) gCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTa < 1:1548765/100‑1 (MQ=255) cTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTaa > 1:2289131/1‑100 (MQ=255) tGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTTTTTTTTTTCTCGCTAAc > 2:843830/1‑100 (MQ=255) gATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCgagatcg > 1:992964/1‑94 (MQ=255) cATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGc > 2:1722801/1‑100 (MQ=255) cATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGc > 2:2044368/1‑100 (MQ=255) cATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGc > 2:596815/1‑100 (MQ=255) tGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCtt < 1:1448774/100‑1 (MQ=255) aaTTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCttatta < 1:2178392/100‑1 (MQ=255) aTTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTtattat > 1:1861075/1‑100 (MQ=255) tcttcCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATggg < 1:2165221/100‑1 (MQ=255) tcttcCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATggg < 2:722342/100‑1 (MQ=255) tcttcCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATggg < 2:721608/100‑1 (MQ=255) cttcCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATgggg < 1:1312679/100‑1 (MQ=255) cttcCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATgggg < 1:1775959/100‑1 (MQ=255) tcCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGAt < 2:243982/100‑1 (MQ=255) ccGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATc > 1:93221/1‑100 (MQ=255) cGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCa < 1:1583550/100‑1 (MQ=255) ggtgCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATc < 2:2216558/95‑1 (MQ=255) ggggCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAg < 2:2001698/100‑1 (MQ=255) ggCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGtt > 2:551276/1‑100 (MQ=255) tGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAg > 2:2304820/1‑100 (MQ=255) cgggatttggCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAgg < 2:512111/92‑1 (MQ=255) ccccTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAggg < 2:290839/100‑1 (MQ=255) ccccTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAggg > 2:654623/1‑100 (MQ=255) cccTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGt > 1:2065104/1‑100 (MQ=255) cTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGttt < 1:1555694/100‑1 (MQ=255) tCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTc > 2:2302507/1‑100 (MQ=255) aGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCaa < 1:2304822/100‑1 (MQ=255) ggCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCaat < 2:897919/100‑2 (MQ=255) ggCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAg > 1:1370963/1‑100 (MQ=255) ggCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAg < 2:2384467/100‑1 (MQ=255) gCGTTGTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAgg > 1:1311009/1‑100 (MQ=255) cGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAggg < 1:1007479/100‑1 (MQ=255) cGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAggg < 2:1534657/100‑1 (MQ=255) cGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTGCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAggg < 2:1534630/100‑1 (MQ=255) ttttCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGaa > 1:2347499/1‑100 (MQ=255) tttCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAg < 1:177546/100‑1 (MQ=255) ttCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGc < 2:278230/100‑1 (MQ=255) ttCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGc < 1:1434851/100‑1 (MQ=255) gTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTc > 1:288459/1‑100 (MQ=255) cTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTcaca > 1:141241/1‑100 (MQ=255) cTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTAGGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTcaca < 2:1288082/100‑1 (MQ=255) tactacTCATGAATTTCTCCGTGTTTTTTTCGTATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTg > 2:469447/1‑100 (MQ=255) tactacTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTg > 1:1556793/1‑100 (MQ=255) tactCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGtca < 1:654622/100‑1 (MQ=255) cgatcttGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGtca < 2:765305/94‑1 (MQ=255) actCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGtcat > 2:1325947/1‑100 (MQ=255) actCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGtcat > 2:1143422/1‑100 (MQ=255) ctCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGtcatc < 1:1722800/100‑1 (MQ=255) cATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCaa > 1:1464442/1‑100 (MQ=255) cATGAATTTCCCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCaa > 2:1340407/1‑100 (MQ=255) aTGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAAt < 1:2044370/100‑1 (MQ=255) tGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGtcaagatcg > 1:765304/1‑94 (MQ=255) tGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATc < 2:1158022/100‑1 (MQ=255) aTTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTc < 2:1556794/100‑1 (MQ=255) tctcCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCCTATTATGGGGATCAGTTTCTGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGca < 2:1311010/100‑1 (MQ=255) tctcCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGca < 2:1631318/100‑1 (MQ=255) ccGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGcaaca > 2:2365434/1‑100 (MQ=255) | CTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACA > NC_000913/2750622‑2750816 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |