Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,714,168 Δ1 bp 17.2% coding (2227/2334 nt) bisC ← biotin sulfoxide reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,714,1680A.17.2% 664.0 / 142.7 238coding (2227/2334 nt)bisCbiotin sulfoxide reductase
Reads supporting (aligned to +/- strand):  ref base A (96/99);  new base . (20/21);  total (118/120)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.51e-02

Only 100 of 240 total aligned reads displayed.

TGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCTTCGTGAATGCAAAT  >  NC_000913/3714069‑3714267
                                                                                                    |                                                                                                   
 ggCCGGTGGTTCAAACGCTGTAAGTTTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAg                                                                                                    >  1:2928758/1‑100 (MQ=255)
 ggCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAg                                                                                                    >  1:3302103/1‑100 (MQ=255)
  gCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGc                                                                                                   >  1:2575258/1‑100 (MQ=255)
     ggtggtTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTgg                                                                                                <  1:2916166/100‑1 (MQ=255)
       tggtTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCTCGCGGCTgggg                                                                                              <  1:3638521/100‑1 (MQ=255)
       tggtTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCTCGCGGCTgggg                                                                                              <  2:301881/100‑1 (MQ=255)
       tggtTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGCGCTgggg                                                                                              >  2:2631937/1‑100 (MQ=255)
       tggtTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTgggg                                                                                              <  2:3150950/100‑1 (MQ=255)
        ggtTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCTCGCGGCTGGGga                                                                                             <  1:1897680/100‑1 (MQ=255)
        ggtTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCTCGCGGCTGGGga                                                                                             <  2:1705095/100‑1 (MQ=255)
        ggtTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGga                                                                                             >  1:3770844/1‑100 (MQ=255)
        ggtTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGga                                                                                             <  2:2203371/100‑1 (MQ=255)
         gtTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGgag                                                                                            >  1:383712/1‑100 (MQ=255)
          ttCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCTCGCGGCTGGGgaat                                                                                           <  1:1691909/100‑3 (MQ=255)
          ttCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCTCGCGGCTGGGgaa                                                                                            <  2:650860/99‑2 (MQ=255)
          ttCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGgag                                                                                            >  2:1165519/1‑99 (MQ=255)
           tCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCTCGCGGCTGGGgaatg                                                                                          <  1:3019793/100‑4 (MQ=255)
           tCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCTCGCGGCTGGGgaatg                                                                                          <  1:3337933/100‑4 (MQ=255)
           tCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCTCGCGGCTGGGgaatg                                                                                          <  2:2558206/100‑4 (MQ=255)
            cAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCTCGCGGCTGGGgaatgg                                                                                         <  1:2501497/100‑5 (MQ=255)
            cAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATc                                                                                         >  1:347452/1‑100 (MQ=255)
             aaaCGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCt                                                                                        >  1:2399476/1‑100 (MQ=255)
             aaaCGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCt                                                                                        <  1:1055684/100‑1 (MQ=255)
             aaaCGCTGTAAGTATCAGTTCCGGACCGTTGAATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCt                                                                                        >  1:2830666/1‑100 (MQ=255)
                cGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCtt                                                                                       <  2:1019341/98‑1 (MQ=255)
                cGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTg                                                                                     <  2:2389784/100‑1 (MQ=255)
                  cTGTACGAGTCAGTTCCGGACCGTTGTAATTTTACAGCCAAGACCGCGCCGTATGTCCCGCACCGCCATTCCCCGGCCGCTATCTGGGGATATCTTTgg                                                                                    <  2:343011/99‑1 (MQ=255)
                    gTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCa                                                                                 <  1:1010988/100‑1 (MQ=255)
                       aGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCa                                                                              <  1:326735/100‑1 (MQ=255)
                       aGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCa                                                                              <  2:2862961/100‑1 (MQ=255)
                        gtgtCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCAc                                                                             <  2:1426710/100‑1 (MQ=255)
                          gtCATTTCCGGACCGTTGTGTGTTTCCAGCCATGCGGGCGCCGTATTACCCGCACATCCATTCCTTAGCCGCGAGCTGGGGAGACCTTTAGTCAGCAc                                                                             <  2:1156193/98‑1 (MQ=255)
                          gtCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGt                                                                           >  1:3941753/1‑100 (MQ=255)
                           tCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGtt                                                                          <  1:3477899/100‑1 (MQ=255)
                           tCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGtt                                                                          <  1:1951306/100‑1 (MQ=255)
                            cAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGCGCTGGGGAGATCTTTGGTCAGCACGTTc                                                                         >  2:3926923/1‑100 (MQ=255)
                            cAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTc                                                                         >  1:433377/1‑100 (MQ=255)
                            cAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTc                                                                         >  2:3369869/1‑100 (MQ=255)
                             aGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCCCAGCCCTTCCCCAGCCGCGAGCTGGGGAGCTCTTTGGTCAGCACGt                                                                           >  2:3907022/1‑97 (MQ=255)
                             aGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCa                                                                        >  2:3175065/1‑100 (MQ=255)
                               tcccGGTCCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCAAAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCAc                                                                       <  2:527170/97‑1 (MQ=255)
                                tCCGCACCGTTGCATTTTTCCAGCCTTCCCATCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGCAGATCTTTGGTCAGCACGTTCACt                                                                      <  2:4058947/99‑1 (MQ=255)
                                 ccGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTgc                                                                    >  2:3101454/1‑100 (MQ=255)
                                  cGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTgcg                                                                   >  1:2094554/1‑100 (MQ=255)
                                     aCCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCg                                                                <  1:3796416/100‑1 (MQ=255)
                                      ccGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGt                                                               >  1:1348225/1‑100 (MQ=255)
                                        gTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGttt                                                             >  1:113280/1‑100 (MQ=255)
                                          tGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGttttt                                                           >  1:2666451/1‑100 (MQ=255)
                                            tATTTTTTCATCCATTCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGCGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTAc                                                         >  2:3975745/1‑100 (MQ=255)
                                            tATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTAc                                                         >  2:291087/1‑100 (MQ=255)
                                            tATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTAc                                                         <  2:347452/100‑1 (MQ=255)
                                             aTTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTAc                                                         <  1:3899224/99‑1 (MQ=255)
                                             aTTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACa                                                        >  1:3689186/1‑100 (MQ=255)
                                             aTTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACa                                                        <  1:2698228/100‑1 (MQ=255)
                                              tttttCCAGCCATGCCAGCGCCGTATTACCCGAACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACaa                                                       <  1:3533276/100‑1 (MQ=255)
                                                  tCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATAc                                                   <  1:89902/100‑1 (MQ=255)
                                                   ccAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTCTGGTCTGCACGTTCACTGCGCCGTTTTTACAAATAcc                                                  >  2:2821418/1‑100 (MQ=255)
                                                    cAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCg                                                 >  1:2796399/1‑100 (MQ=255)
                                                      gCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGACCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTc                                               >  2:1636302/1‑100 (MQ=255)
                                                         aTGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGc                                            >  1:395037/1‑100 (MQ=255)
                                                         aTGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGc                                            >  1:1531636/1‑100 (MQ=255)
                                                          tGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCg                                           <  2:461438/100‑1 (MQ=255)
                                                            ccAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGt                                         >  1:582408/1‑100 (MQ=255)
                                                              aGCGCCGTATTACCCGCACAGCCATTCCCCAGTCGCGAGGTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTa                                       <  2:2939374/100‑1 (MQ=255)
                                                               gcgcCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTaa                                      >  1:2242449/1‑100 (MQ=255)
                                                                cgcCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTaaa                                     <  1:31126/100‑1 (MQ=255)
                                                                   cGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATcc                                  <  2:2157019/100‑1 (MQ=255)
                                                                   cGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATcc                                  <  1:934710/100‑1 (MQ=255)
                                                                    gTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCa                                 <  1:3101466/100‑1 (MQ=255)
                                                                    gTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCa                                 <  1:653641/100‑1 (MQ=255)
                                                                    gTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCa                                 >  2:2129160/1‑100 (MQ=255)
                                                                    gTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCa                                 <  1:245057/100‑1 (MQ=255)
                                                                    cttccgatctcacaGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCa                                 <  1:3594672/90‑1 (MQ=255)
                                                                      aTTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGa                               >  1:207458/1‑100 (MQ=255)
                                                                       ttACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGAt                              <  1:4158060/100‑1 (MQ=255)
                                                                          cccGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATGTTTGGTCAGGACGGTCACTGCGCGGTGTTTACAAATACAGTAAGCGGGTAAAGCCa                                 >  2:3790371/1‑94 (MQ=255)
                                                                          cccGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCg                           >  1:2336187/1‑100 (MQ=255)
                                                                           ccGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCgg                          >  1:1893307/1‑100 (MQ=255)
                                                                            cGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGc                         >  2:2296153/1‑100 (MQ=255)
                                                                              cacaGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCaagatcggaag                       >  2:3594655/1‑90 (MQ=255)
                                                                              cacaGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCa                       <  2:3570661/100‑1 (MQ=255)
                                                                                caGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATg                     >  1:1224128/1‑100 (MQ=255)
                                                                                  gCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGcc                   <  2:150841/100‑1 (MQ=255)
                                                                                  gCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGcc                   >  1:2408865/1‑100 (MQ=255)
                                                                                   gatctCCCCAGCTCGCGGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGccc                  >  2:93770/5‑100 (MQ=255)
                                                                                      ctCCCCAGCTCGCGGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCtt               >  2:2482443/2‑100 (MQ=255)
                                                                                       tCCCCAGCTCGCGGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCTTc              >  2:3260518/1‑100 (MQ=255)
                                                                                       tCCCCAGCTCGCGGCTGGGGAGATCTTTGGTCAACACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCGATGCCCCTTc              >  2:2762725/1‑100 (MQ=255)
                                                                                        ccccAGCTCGCGGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCTTCg             >  1:4110163/1‑100 (MQ=255)
                                                                                        ccccAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCTTCg             >  2:1055976/1‑100 (MQ=255)
                                                                                        ccccAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCTTCg             >  1:793497/1‑100 (MQ=255)
                                                                                           cAGCCGCGAGCTGGGGAGATCTTTGGTCAGCATGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCTTCGTg           <  2:1904167/99‑1 (MQ=255)
                                                                                           cAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCTTCGTGa          >  2:4013976/1‑100 (MQ=255)
                                                                                             gCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCTTCGTGAAt        <  1:156290/100‑1 (MQ=255)
                                                                                              ccGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCTTCGTGAATg       >  2:306389/1‑100 (MQ=255)
                                                                                              ccGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCTTCGTGAATg       <  2:1980472/100‑1 (MQ=255)
                                                                                              ccGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCTTCGTGAATg       <  2:1397109/100‑1 (MQ=255)
                                                                                                cgcgAGCTGGGGAGATCTTTTGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCTTCGTGAATg       <  2:1178208/99‑1 (MQ=255)
                                                                                                cgcgAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCTTCGTGAATGc      >  1:2628030/1‑100 (MQ=255)
                                                                                                    aGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCTTCGTGAATGCAAAt  <  2:798563/100‑1 (MQ=255)
                                                                                                    |                                                                                                   
TGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCTTCGTGAATGCAAAT  >  NC_000913/3714069‑3714267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: