Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,956,978 Δ1 bp 15.2% coding (843/894 nt) ilvY ← transcriptional activator of ilvC; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,956,9780T.15.2% 508.5 / 88.3 171coding (843/894 nt)ilvYtranscriptional activator of ilvC; autorepressor
Reads supporting (aligned to +/- strand):  ref base T (73/72);  new base . (8/18);  total (81/90)
Fisher's exact test for biased strand distribution p-value = 8.76e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 176 total aligned reads displayed.

AAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTT  >  NC_000913/3956879‑3957077
                                                                                                    |                                                                                                   
aaaaTGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGt                                                                                                     >  1:1402510/1‑100 (MQ=255)
 aaaTGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGATCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCGCATGTa                                                                                                    >  2:691537/1‑100 (MQ=255)
   aTGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGc                                                                                                  >  1:2805411/1‑100 (MQ=255)
       cTGATAGCGCTTCGCTTATCAGGCCTACCCGCTCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTACATGAGCCGCt                                                                                              <  2:588814/100‑1 (MQ=255)
       cTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTACATGAGCCGCt                                                                                              <  2:551523/100‑1 (MQ=255)
       cTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCt                                                                                              <  1:719637/100‑1 (MQ=255)
        tGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCtt                                                                                             >  1:1491218/1‑100 (MQ=255)
        tGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCCGCTCATGTAGCCGCtt                                                                                             >  2:2449807/1‑100 (MQ=255)
        tGATAGCGCTTCGCTTATCACGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCtt                                                                                             <  2:1912060/100‑1 (MQ=255)
          aTAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTACATGAGCCGCtaat                                                                                           <  1:1246756/100‑4 (MQ=255)
          aTAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTACATGAGCCGCtaat                                                                                           <  1:551254/100‑4 (MQ=255)
          aTAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCtttt                                                                                           <  2:378218/100‑1 (MQ=255)
           tAGCTCTTTGCTTATCAGGCCTACCCGCGCGACAAGGTCATTGGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTACATGAGCCGCtaatt                                                                                          <  2:3387186/100‑5 (MQ=255)
             gcgcTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTACATGAGCCGCtaattga                                                                                        <  1:1700217/100‑7 (MQ=255)
             gcgcTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCt                                                                                        >  1:1277766/1‑100 (MQ=255)
             gcgcTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCt                                                                                        >  2:3313392/1‑100 (MQ=255)
             gcgcTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCt                                                                                        >  1:520400/1‑100 (MQ=255)
              cgcttcgcttATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTg                                                                                       <  2:2222990/100‑1 (MQ=255)
               gcttcgcttATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTACATGAGCCGCtaattgagg                                                                                      <  1:3269484/100‑9 (MQ=38)
               gcttcgcttATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTACATGAGCCGCtaattgagg                                                                                      <  1:1241314/100‑9 (MQ=38)
               gcttcgcttATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTACATGAGCCGCtaattgagg                                                                                      <  2:2302923/100‑9 (MQ=38)
               gcttcgcttATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTgc                                                                                      <  2:2095421/100‑1 (MQ=255)
               gcttcgcttATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTgc                                                                                      <  1:1873422/100‑1 (MQ=255)
                cttcgcttATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATTCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTgcg                                                                                     >  1:3991140/1‑100 (MQ=255)
                  tcgcttATCATGCCTAAGCGCTCCAAACCGTCATTTGTGGTTCGGCAAAATCTTCCATAATGCCTCAATTAGCGGCTACATGAGCCGCtaattgaggc                                                                                     <  2:958850/98‑10 (MQ=16)
                  tcgcttATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGca                                                                                   <  1:645078/100‑1 (MQ=255)
                  tcgcttATCAGGCCTACCCGCGCGACAACGCCATTTGTGGTTCGGCAAACTCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGca                                                                                   >  2:1188592/1‑100 (MQ=255)
                    gcttATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGcaca                                                                                 <  1:1863324/100‑1 (MQ=255)
                    gcttATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGcaca                                                                                 <  2:1613349/100‑1 (MQ=255)
                     catATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGcacac                                                                                >  1:2831810/3‑100 (MQ=255)
                      ttATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGcacaca                                                                               <  1:1588074/100‑1 (MQ=255)
                       tATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCacacac                                                                              >  1:3028738/1‑100 (MQ=255)
                       tATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCacacac                                                                              >  2:1085155/1‑100 (MQ=255)
                          cAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGcc                                                                           >  2:2973016/1‑100 (MQ=255)
                          cAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGcc                                                                           >  1:966093/1‑100 (MQ=255)
                            ggCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCa                                                                         >  2:3951759/1‑100 (MQ=255)
                              ccTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGc                                                                       <  2:402213/100‑1 (MQ=255)
                              ccTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGc                                                                       >  2:630198/1‑100 (MQ=255)
                               cTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCt                                                                      <  2:3787123/100‑1 (MQ=255)
                                tACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGc                                                                       <  2:2542496/98‑1 (MQ=255)
                                 aCCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCa                                                                    >  2:2292715/1‑100 (MQ=255)
                                  cccGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCaa                                                                   >  1:3914548/1‑100 (MQ=255)
                                  cccGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCa                                                                    >  2:3669643/1‑99 (MQ=255)
                                   ccGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCaaa                                                                  >  1:3031880/1‑100 (MQ=255)
                                   ccGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTACAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACAAACGCCCAGCTCaaa                                                                  >  2:1040801/1‑100 (MQ=255)
                                    cgcgcgACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAAc                                                                 >  1:1934558/1‑100 (MQ=255)
                                    cgcgcgACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAAc                                                                 <  2:3877155/100‑1 (MQ=255)
                                       gcgACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGc                                                              <  1:224063/100‑1 (MQ=255)
                                        cgACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCg                                                             <  1:1086338/100‑1 (MQ=255)
                                        cgACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCg                                                             <  1:3159100/100‑1 (MQ=255)
                                           cAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGttt                                                          <  1:797526/100‑1 (MQ=255)
                                           cAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGttt                                                          >  1:3395970/1‑100 (MQ=255)
                                           cAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGttt                                                          <  1:3775935/100‑1 (MQ=255)
                                            aaCGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGtttt                                                         >  2:421131/1‑100 (MQ=255)
                                             tcGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTtc                                                        <  2:4042907/99‑1 (MQ=255)
                                              cGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTtct                                                       >  1:2361206/1‑100 (MQ=255)
                                               gTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTtctc                                                      <  1:2431550/100‑1 (MQ=255)
                                                tCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCa                                                     >  1:2372477/1‑100 (MQ=255)
                                                  aTTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATc                                                   <  1:1318360/100‑1 (MQ=255)
                                                   tttGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCg                                                  <  1:3671031/100‑1 (MQ=255)
                                                    ttGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGc                                                 <  2:2590676/100‑1 (MQ=255)
                                                      gtgGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTg                                               <  1:3091720/100‑1 (MQ=255)
                                                       tgGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTgc                                              >  2:531025/1‑100 (MQ=255)
                                                             ggCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTa                                        >  1:2477920/1‑100 (MQ=255)
                                                              gCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTaa                                       >  2:110857/1‑100 (MQ=255)
                                                              gCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTATCTCATCGCTGCGCTCTaa                                       >  1:892376/1‑100 (MQ=255)
                                                               cAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCATACGGCGTTTTCTCATCGCTGCGCTCTaaa                                      >  2:2524379/1‑100 (MQ=255)
                                                                    tCTTCCAGAATGCCTCCATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACGCGCCCAGCTCAAACGGCGTTTTCTGATCGCTGCGCTCTACAatca                                  <  2:1553537/99‑1 (MQ=255)
                                                                    tCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAatcat                                 <  1:3313407/100‑1 (MQ=255)
                                                                         ccgAATGTCTCAATTACCGGCTCATGTAGCCGCTTTTTCTCCGCACACACGCCCAGCTCAAACCGCGTTTTCTCATCGCTGCGCTCTAAAATAATCAggc                            >  2:3152178/3‑100 (MQ=255)
                                                                          aGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACGCGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAAGCAGCAcgcg                           <  2:820981/100‑1 (MQ=255)
                                                                             aTGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGtt                        >  2:3098063/1‑100 (MQ=255)
                                                                              gcgcagaaaaaGCGGCTACATGAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTa                       >  2:1736021/11‑100 (MQ=37)
                                                                                ccTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTatg                     <  2:235849/100‑3 (MQ=255)
                                                                                ccTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACg                     <  2:2477914/100‑1 (MQ=255)
                                                                                ccTCAATTAGCGGCCCATGTAGCCGCTTTTTCTGCGCACACACGCCCCGCTCAACCGGCGCTTTCTCATCCCTGCGCTCTAAAATCATCACGCGGTTCCg                     >  1:116529/1‑100 (MQ=255)
                                                                                 cTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACGCGCCCAGCTCAAACGGCGTCTTCTCACCGCTGCGCTCTAAAATCATCACGCGGTTACGc                    <  2:281237/100‑1 (MQ=255)
                                                                                 cTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGc                    <  1:2014323/100‑1 (MQ=255)
                                                                                 cTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGc                    >  1:110806/1‑100 (MQ=255)
                                                                                 cTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGc                    <  1:456509/100‑1 (MQ=255)
                                                                                   gaaaaaGCGGCTACATGAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCAc                  >  1:1089156/6‑100 (MQ=255)
                                                                                    aaTTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCAcc                 <  2:2802031/100‑1 (MQ=255)
                                                                                    aaTTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCAcc                 <  1:2922717/100‑1 (MQ=255)
                                                                                    aaTTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCAcc                 <  2:1441582/100‑1 (MQ=255)
                                                                                     aaaaGCGGCTACATGAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCg                >  2:779704/4‑100 (MQ=255)
                                                                                          cGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCg           <  2:858419/100‑1 (MQ=255)
                                                                                           ggCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCgg          >  1:233250/1‑100 (MQ=255)
                                                                                           ggCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCgg          <  1:2555/100‑1 (MQ=255)
                                                                                            gCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCggg         >  1:4182595/1‑100 (MQ=255)
                                                                                            gCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCggg         <  1:674371/100‑1 (MQ=255)
                                                                                             ntCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCgggg        >  1:229834/2‑100 (MQ=255)
                                                                                             cTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCgggg        <  2:3603804/100‑1 (MQ=255)
                                                                                              tCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGc       <  2:527278/100‑1 (MQ=255)
                                                                                                 aTGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTg     <  1:2712241/100‑1 (MQ=255)
                                                                                                  tGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTg     >  1:4044889/1‑99 (MQ=255)
                                                                                                  tGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGt    >  1:3855204/1‑100 (MQ=255)
                                                                                                  tGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGt    >  1:2207388/1‑100 (MQ=255)
                                                                                                  tGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGt    <  2:1432885/100‑1 (MQ=255)
                                                                                                   gTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGtt   <  1:3974196/100‑1 (MQ=255)
                                                                                                    tAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGttt  >  1:3749114/1‑100 (MQ=255)
                                                                                                    |                                                                                                   
AAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTT  >  NC_000913/3956879‑3957077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: