Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 256,459 G→A 19.7% intergenic (+24/‑68) gpt → / → frsA xanthine phosphoribosyltransferase; xanthine‑guanine phosphoribosyltransferase/fermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913256,4590GA19.7% 398.3 / 77.4 177intergenic (+24/‑68)gpt/frsAxanthine phosphoribosyltransferase; xanthine‑guanine phosphoribosyltransferase/fermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity
Reads supporting (aligned to +/- strand):  ref base G (84/58);  new base A (22/13);  total (106/71)
Fisher's exact test for biased strand distribution p-value = 8.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 186 total aligned reads displayed.

ATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTT  >  NC_000913/256361‑256558
                                                                                                  |                                                                                                   
atCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCgg                                                                                                    <  2:4073760/100‑1 (MQ=255)
atCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGAGCGTCGTATTCGTCCCGCCAATTACCGGTTGCTAATCTTTTCAACGCCTGGCACTGCCgg                                                                                                    >  1:2656695/1‑100 (MQ=255)
 tCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCggg                                                                                                   <  2:579570/100‑1 (MQ=255)
 tCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCggg                                                                                                   <  1:4017806/100‑1 (MQ=255)
  cccGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGc                                                                                                  >  1:2246304/1‑100 (MQ=255)
   ccGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCg                                                                                                 >  1:2419779/1‑100 (MQ=255)
   ccGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCg                                                                                                 >  1:4140428/1‑100 (MQ=255)
    cGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCgt                                                                                                >  1:3447083/1‑100 (MQ=255)
     gCAAGATACCTGGATTGAACAGCCGTGTGATATGGGCGTCGTATTCGTCCCGCCAATCTCCCGTCGCTAATCTTTTCAACGCCCGGCAGTGCCAGGCgtt                                                                                               <  1:10084/100‑1 (MQ=255)
      gatcttACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCCGGCAGTGCCAGGCgttg                                                                                              <  2:2518675/95‑1 (MQ=255)
      cAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCgttg                                                                                              <  1:684384/100‑1 (MQ=255)
        aGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCgttg                                                                                              <  2:3852097/98‑1 (MQ=255)
        aGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCCGGCAGTGCCAGGCgttga                                                                                             <  2:2528050/99‑2 (MQ=39)
         gATACCTGGATTGACCCGCCGTGGGGGATGGGCGTCGTATTCGTCCCGCGAGGCTCCGGTCGCTAATCTTTGCAACGCCTGGCACTGCCGGGCgttg                                                                                              <  2:3933256/97‑1 (MQ=255)
         gATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGGCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCCGGCAGTGCCAGGCgttga                                                                                             <  2:2855203/98‑2 (MQ=38)
           tACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCtt                                                                                         >  1:1351031/1‑100 (MQ=255)
           tACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCtt                                                                                         <  2:3161303/100‑1 (MQ=255)
           tACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCtt                                                                                         >  2:2608895/1‑100 (MQ=255)
           tACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCCGGCAGTGCCAGGCgttgagatc                                                                                         >  1:522881/1‑95 (MQ=38)
             ccTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTGGTTCttt                                                                                        >  2:343276/1‑99 (MQ=255)
             ccTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTAGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCtttt                                                                                       >  2:2686771/1‑100 (MQ=255)
              cTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCttttt                                                                                      <  2:2318105/100‑1 (MQ=255)
              cTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCttttt                                                                                      <  1:1120116/100‑1 (MQ=255)
              cTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCttttt                                                                                      >  1:1789742/1‑100 (MQ=255)
              cTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTCGTTCttttt                                                                                      >  2:3541544/1‑100 (MQ=255)
               tGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTa                                                                                     >  2:3414322/1‑100 (MQ=255)
               tGGATTGAACAGCCGTGGGAGATGGGCTTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTa                                                                                     <  2:2398964/100‑1 (MQ=255)
                ggATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTaa                                                                                    <  1:3959950/100‑1 (MQ=255)
                ggATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTaa                                                                                    <  1:3865161/100‑1 (MQ=255)
                  aTTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACt                                                                                  <  1:802433/100‑1 (MQ=255)
                    tGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTc                                                                                <  2:3299737/100‑1 (MQ=255)
                    tGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTc                                                                                >  1:3503816/1‑100 (MQ=255)
                    tGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGGCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTc                                                                                >  1:3929599/1‑100 (MQ=255)
                     gAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCa                                                                               <  1:2103700/100‑1 (MQ=255)
                     gAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCa                                                                               >  1:1748509/1‑100 (MQ=255)
                     gAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCa                                                                               >  2:2719968/1‑100 (MQ=255)
                      aaCAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAg                                                                              >  2:136735/1‑100 (MQ=255)
                        cAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCACTCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTACCGGGCGTTGTTCTTTTTAACTTCAGGc                                                                            >  2:1767795/1‑100 (MQ=255)
                        cAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGc                                                                            >  1:37289/1‑100 (MQ=255)
                          gCCGTTGGATATGGGCGTCGTATTCGTCGCGCTAAGCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGc                                                                            <  2:721723/98‑1 (MQ=255)
                          gCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCgg                                                                          >  2:506732/1‑100 (MQ=255)
                           ccGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCggg                                                                         <  1:1469350/100‑1 (MQ=255)
                            cGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGt                                                                        <  1:2800379/100‑1 (MQ=255)
                                 gATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCATGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTCCaa                                                                   >  1:1678941/1‑100 (MQ=255)
                                     tGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGt                                                               >  2:2239077/1‑100 (MQ=255)
                                      gggCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGtt                                                              <  1:3964867/100‑1 (MQ=255)
                                         cgtcgtATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTcc                                                           <  1:480534/100‑1 (MQ=255)
                                         cgtcgtATTCGTCCCGCCAATCACCGGTCGCTAATCTTGTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGTTTAAACTAGTTTcc                                                           >  2:3977693/1‑100 (MQ=255)
                                           tcgtATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCag                                                         <  1:1256895/100‑1 (MQ=255)
                                            cgtATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCagt                                                        >  2:713647/1‑100 (MQ=255)
                                               aTTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCagtaag                                                     <  1:4099543/100‑1 (MQ=255)
                                               aTTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCagtaag                                                     <  2:3590830/100‑1 (MQ=255)
                                               aTTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCagtaag                                                     <  1:3690886/100‑1 (MQ=255)
                                               aTTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCagtaag                                                     >  2:1696974/1‑100 (MQ=255)
                                               aTTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCAATGCCAGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCagtaa                                                      >  2:2325245/1‑99 (MQ=255)
                                                ttCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCagtaagt                                                    <  1:4054187/100‑1 (MQ=255)
                                                   gTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTAtt                                                 >  1:1912086/1‑100 (MQ=255)
                                                     nccGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCt                                               >  2:2218594/2‑100 (MQ=255)
                                                      ccGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCt                                               >  1:277945/1‑99 (MQ=255)
                                                      ccGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTg                                              >  2:3020228/1‑100 (MQ=255)
                                                             tctcCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTg                                       >  1:2674313/1‑100 (MQ=255)
                                                              ctcCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGc                                      <  2:3819846/100‑1 (MQ=255)
                                                               tcCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCa                                     >  1:553367/1‑100 (MQ=255)
                                                                ccGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCAt                                    >  2:2604530/1‑100 (MQ=255)
                                                                  ggTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATcc                                  >  1:6111/1‑100 (MQ=255)
                                                                     cGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATg                               <  1:331034/100‑1 (MQ=255)
                                                                        taaaaagaacAACGCCCGGCAGTGCCAGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGaca                            >  1:1286882/10‑100 (MQ=37)
                                                                         aaTCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGacac                           >  2:19200/1‑100 (MQ=255)
                                                                          aaaagaacAACGCCCGGCAGTGCCAGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGAcaca                          >  2:2838220/8‑100 (MQ=38)
                                                                          aTCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGAcaca                          >  1:893741/1‑100 (MQ=255)
                                                                           tCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAg                         <  1:218610/100‑1 (MQ=255)
                                                                           tCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAg                         >  2:1623922/1‑100 (MQ=255)
                                                                            cTTTTGAGCGGCTGGCAGTGCCGGGCGTGGTTCTTTTTAACTTCGGGGGGGTTACAATAGTTTCCCGGAGGTATTCTGGAGGCTGCGTCCCTGAcaca                          <  2:2194172/98‑1 (MQ=255)
                                                                            cTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAgg                        >  2:35626/1‑100 (MQ=255)
                                                                            cTTTTCAACGCCTGGCACTGCCGGGCGTTATTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAgg                        <  2:3159366/100‑1 (MQ=255)
                                                                            cTTTTCAACGCCTGGCACTGCCGGGAGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAgg                        >  1:561494/1‑100 (MQ=255)
                                                                             ttttCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGc                       <  1:3686609/100‑1 (MQ=255)
                                                                             ttttCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGc                       >  2:4113994/1‑100 (MQ=255)
                                                                             agaacAACGCCCGGCAGTGCCAGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAg                         >  2:3656224/5‑98 (MQ=38)
                                                                               aacAACGCCCGGCAGTGCCAGGCGTTGTTCTTTTTAACTTCAGCCGGGTTACAATAGTTTCCAGTAAGTATTCTAGAGGCTGCATCCATGACACAGCCaa                     >  2:2790243/3‑100 (MQ=38)
                                                                                acAACGCCCGGCAGTGCCAGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCaaa                    >  1:364106/2‑100 (MQ=39)
                                                                                 cAACGCCCGGCAGTGCCAGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAAc                   >  2:1683639/1‑100 (MQ=255)
                                                                                  aaCGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAAcc                  >  1:2730342/1‑100 (MQ=255)
                                                                                  aaCGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAAcc                  <  1:1623926/100‑1 (MQ=255)
                                                                                  aaCGCCCGGCAGTGCCAGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGCGGCTGCATCCATGACACAGGCAAAcc                  >  1:1217707/1‑100 (MQ=255)
                                                                                  aaCGCCCGGCAGTGCCAGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAAcc                  >  2:1196721/1‑100 (MQ=255)
                                                                                  aaCGCCCGGCAGTGCCAGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAAcc                  >  2:1180958/1‑100 (MQ=255)
                                                                                   aCGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCt                 >  2:3503507/1‑100 (MQ=255)
                                                                                   aCGCCCGGCAGTGCCAGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCTGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCt                 >  2:3456892/1‑100 (MQ=255)
                                                                                   aCGCCCGGCAGTGCCAGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCt                 >  1:959219/1‑100 (MQ=255)
                                                                                   aCGCCCGGCAGTGCCAGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCt                 >  1:958714/1‑100 (MQ=255)
                                                                                    cGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGCGGCTGCATCCATTACACAGGCAAAcat                 >  1:302663/1‑97 (MQ=255)
                                                                                    cGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTg                <  1:601963/100‑1 (MQ=255)
                                                                                      ccTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAg              >  1:2061949/1‑100 (MQ=255)
                                                                                        tGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCg            >  1:626251/1‑100 (MQ=255)
                                                                                           cACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGaaa         >  1:47049/1‑100 (MQ=255)
                                                                                            aCTGCCGGGCGTTGTTCTTTTTAACTTCAGGCTGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAAc        <  2:1333910/100‑1 (MQ=255)
                                                                                               gCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCt     >  2:2630342/1‑100 (MQ=255)
                                                                                               gCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAAGCTGCATCCATGACACAGGCAAACCTGAACGAAACCCt     >  2:486514/1‑100 (MQ=255)
                                                                                                 cGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGt   <  1:258698/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
ATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTT  >  NC_000913/256361‑256558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: