Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,255,991 A→T 19.7% K213* (AAA→TAA) ‡ yeiL → putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,255,9910AT19.7% 373.7 / 85.4 169K213* (AAA→TAA) yeiLputative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (77/58);  new base T (19/14);  total (96/73)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.36e-01

Only 100 of 172 total aligned reads displayed.

TCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGC  >  NC_000913/2255893‑2256090
                                                                                                  |                                                                                                   
  attcaCGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAAt                                                                                                  <  1:1019007/100‑1 (MQ=255)
  attcaCGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAAt                                                                                                  <  2:2922322/100‑1 (MQ=255)
    tcaCGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATtc                                                                                                >  1:1677360/1‑100 (MQ=255)
     caCGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATtct                                                                                               >  2:2087245/1‑100 (MQ=255)
     caCGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATtct                                                                                               >  2:1072869/1‑100 (MQ=255)
         acgGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCgg                                                                                           <  2:91832/100‑1 (MQ=255)
          cgGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATTTATTCTCCggg                                                                                          <  1:3780700/100‑1 (MQ=255)
          cgGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATTTATTCTCCggg                                                                                          <  1:1944911/100‑1 (MQ=255)
          cgGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCgg                                                                                           <  2:247007/99‑1 (MQ=255)
           gtttgagtaattaaAAGCAAGAAATGGGATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATTTATTCTCCgggt                                                                                         <  2:2422781/93‑2 (MQ=255)
           ggTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATTTATTCTCCgggt                                                                                         <  2:1101003/100‑2 (MQ=255)
           ggTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATTTATTCTCCgggt                                                                                         <  1:2902602/100‑2 (MQ=255)
           ggTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATTTATTCTCCgggt                                                                                         <  1:4031105/100‑2 (MQ=255)
              ttattaATTAAAAGCAAGAACGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGgatga                                                                                      >  2:2564966/1‑100 (MQ=255)
               tattaATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCCATTGTCAGGAATGGCGCTGGAGATGGACACGGAGAATAAATTCTCCGGgatgat                                                                                     >  2:1994457/1‑100 (MQ=255)
                    attaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGt                                                                                >  1:4044529/1‑100 (MQ=255)
                    attaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCGGGAGATGAACCCGGAGAATAAATTCTCCGGGATGATGCAGt                                                                                >  1:2496847/1‑100 (MQ=255)
                     ttaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTa                                                                               <  2:1674055/100‑1 (MQ=255)
                     ttaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTa                                                                               >  1:3674481/1‑100 (MQ=255)
                       aaaaGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGATGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTa                                                                               >  2:3921620/1‑98 (MQ=255)
                        aaaGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTaaaa                                                                            <  1:2493104/100‑1 (MQ=255)
                          aGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAat                                                                          <  1:3319928/100‑1 (MQ=255)
                           gCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGCTGGACCCGGAGAATAAATTCTCCGGGAGGAAGCAGTAAAAatt                                                                         >  2:3910224/1‑100 (MQ=255)
                            cAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGACAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAatta                                                                        >  2:3268936/1‑100 (MQ=255)
                             aaGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAattat                                                                       >  1:682617/1‑100 (MQ=255)
                              aGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAattatt                                                                      <  1:2084651/100‑1 (MQ=255)
                               gAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTAttt                                                                     <  2:2308516/100‑1 (MQ=255)
                                 aaGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTc                                                                   <  1:3348171/100‑1 (MQ=255)
                                 aaGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTc                                                                   >  1:708801/1‑100 (MQ=255)
                                  aagttATGTCATTATAGCCAGAAAGCAGTTGTAATTACCGGCGCTGGAGATGGAACCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTc                                                                   <  2:2408635/95‑1 (MQ=255)
                                     gTATCTCATTAAGAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATa                                                               >  1:2748983/1‑100 (MQ=255)
                                       aTCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAgc                                                             >  1:3002857/1‑100 (MQ=255)
                                         ctcATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAgcgc                                                           <  2:3838014/100‑1 (MQ=255)
                                         ctcATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAgcgc                                                           >  2:3163795/1‑100 (MQ=255)
                                            aTTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGa                                                         >  1:1153660/1‑99 (MQ=255)
                                             ttAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGAtt                                                       >  2:1824083/1‑100 (MQ=255)
                                               aaaaaCAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGc                                                     <  2:1164400/100‑1 (MQ=255)
                                                aaaaCAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGcc                                                    <  2:701094/100‑1 (MQ=255)
                                                aaaaCAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGcc                                                    <  1:1295425/100‑1 (MQ=255)
                                                aaaaCAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGcc                                                    <  1:40533/100‑1 (MQ=255)
                                                 aaaCAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCg                                                   <  1:2868828/100‑1 (MQ=255)
                                                  aaCAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCgg                                                  <  2:565251/100‑1 (MQ=255)
                                                    cAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGAt                                                <  2:4044502/100‑1 (MQ=255)
                                                    cAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGAt                                                >  1:891843/1‑100 (MQ=255)
                                                    cAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGAt                                                <  2:4047449/100‑1 (MQ=255)
                                                     aGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATg                                               <  1:3501372/100‑1 (MQ=255)
                                                      gAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGc                                              >  1:569983/1‑100 (MQ=255)
                                                         aGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTCCAATAGCGCGATTGCCGGATGCAAc                                           <  1:1588510/100‑1 (MQ=255)
                                                            aGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTGCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCt                                        >  1:2417854/1‑100 (MQ=255)
                                                            aGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCt                                        >  1:900110/1‑100 (MQ=255)
                                                             gTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCtt                                       >  1:730793/1‑100 (MQ=255)
                                                              tggTCAGTGCTGGCGCTGGAGATGGGCACGGAGAATAAATTCTCCGGCAAGATGCTGTAAAAATTATTTGCAATAGCGCGATTGCCGGATGCAACGCTTa                                      <  2:4159171/98‑1 (MQ=255)
                                                                 tCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATa                                   <  1:2792557/100‑1 (MQ=255)
                                                                  cAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATAc                                  >  2:3472006/1‑100 (MQ=255)
                                                                   aGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATAc                                  >  1:3852364/1‑99 (MQ=255)
                                                                    ggACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGt                                >  1:4135239/1‑100 (MQ=255)
                                                                     gACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGtt                               <  2:3131615/100‑1 (MQ=255)
                                                                      aCTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGttt                              <  2:3117308/100‑1 (MQ=255)
                                                                      aCTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGttt                              <  1:1371711/100‑1 (MQ=255)
                                                                      aCTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGttt                              >  2:2054605/1‑100 (MQ=255)
                                                                        tGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTa                            <  1:412084/100‑1 (MQ=255)
                                                                         ggCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTa                            <  1:3163809/99‑1 (MQ=255)
                                                                         ggCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTAt                           >  1:1898828/1‑100 (MQ=255)
                                                                         ggCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTAt                           >  2:2656138/1‑100 (MQ=255)
                                                                         ggCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTAt                           >  1:757175/1‑100 (MQ=255)
                                                                         ggCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTAt                           >  1:3237562/1‑100 (MQ=255)
                                                                           cgcTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATcc                         >  2:189804/1‑100 (MQ=255)
                                                                           cgcTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATcc                         >  1:3698204/1‑100 (MQ=255)
                                                                           cgcTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATcc                         >  2:2173920/1‑100 (MQ=255)
                                                                           cgcTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATcc                         >  1:3567314/1‑100 (MQ=255)
                                                                            gcTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCg                        >  1:3660244/1‑100 (MQ=255)
                                                                             cTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCgg                       <  1:1027476/100‑1 (MQ=255)
                                                                             cTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCgg                       <  1:681931/100‑1 (MQ=255)
                                                                               ggAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTc                     <  2:334297/100‑1 (MQ=255)
                                                                               ctgcatcatcccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTc                     >  1:3491798/10‑100 (MQ=38)
                                                                                gagaTGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCt                    <  1:400916/100‑1 (MQ=255)
                                                                                cgcatcatcccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCt                    <  2:3491781/92‑1 (MQ=38)
                                                                                 agaTGGACCCGGAGAATAAATTCTCCGGGATGATGCATTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTa                   >  2:2234435/1‑100 (MQ=255)
                                                                                 agaTGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTa                   >  1:1282047/1‑100 (MQ=255)
                                                                                 agaTGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTa                   >  2:491087/1‑100 (MQ=255)
                                                                                     ggACCCGGAGAATAAATTCTCCAGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACaaa               <  1:745794/100‑1 (MQ=255)
                                                                                     gatcccGGAGAATTTATTCTGCGGGATGATGCAGTAAAAATTATTTTCAATATCGCGATTGCCGGTGGCAACGCATAATCCGCATTATCCCGTcccc                  >  2:779589/4‑94 (MQ=255)
                                                                                      atcccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAAt              >  1:553937/3‑100 (MQ=255)
                                                                                       tcccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCACTAGCGCGATCGCCGCATGCAACGCTTCATAAGTTTTATCCGGTCTACAAAt              >  2:3444622/2‑99 (MQ=255)
                                                                                       tcccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTTTACAAAt              >  2:3444609/2‑99 (MQ=255)
                                                                                       tcccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATc             >  1:3991146/2‑100 (MQ=255)
                                                                                       aCCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATc             >  1:2926295/1‑100 (MQ=255)
                                                                                        cccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCg            >  2:3528231/1‑100 (MQ=255)
                                                                                        cccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCg            >  1:3405307/1‑100 (MQ=255)
                                                                                        cccGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAAt              <  2:3597956/98‑1 (MQ=255)
                                                                                         ccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGa           >  1:2693145/1‑100 (MQ=255)
                                                                                         ccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGa           >  1:1767789/1‑100 (MQ=255)
                                                                                         ccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGa           >  2:861972/1‑100 (MQ=255)
                                                                                         ccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAAGACGTTTTATCCGGTCTACAAATCGa           >  2:1462773/1‑100 (MQ=255)
                                                                                          cGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGCTTGCCGGATGCAACGCTTAATAAGTTTTATGCGGTCCACAAATCGa           >  2:2846269/1‑99 (MQ=255)
                                                                                          cGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAg          <  1:923663/100‑1 (MQ=255)
                                                                                           ggAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTagatcggaaga         >  1:3845770/1‑90 (MQ=255)
                                                                                                aTAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTAc    >  2:1256257/1‑100 (MQ=255)
                                                                                                 tAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCAGTCTACAAATCGAGCATTACg   >  1:820222/1‑100 (MQ=255)
                                                                                                  aaaTTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGc  >  1:1911828/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
TCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGC  >  NC_000913/2255893‑2256090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: