Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,483,906 T→A 16.8% I51N (ATT→AAT)  evgA → response regulator in two‑component regulatory system with EvgS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,483,9060TA16.8% 374.8 / 64.0 150I51N (ATT→AAT) evgAresponse regulator in two‑component regulatory system with EvgS
Reads supporting (aligned to +/- strand):  ref base T (58/66);  new base A (14/11);  total (73/77)
Fisher's exact test for biased strand distribution p-value = 5.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

Only 100 of 158 total aligned reads displayed.

TTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGA  >  NC_000913/2483808‑2484005
                                                                                                  |                                                                                                   
 ttATTGATCAAAAACTATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTg                                                                                                   <  1:4128762/100‑1 (MQ=255)
 ttATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTg                                                                                                   <  2:1320261/100‑1 (MQ=255)
 ttATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTg                                                                                                   <  1:2069256/100‑1 (MQ=255)
  tATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGa                                                                                                  <  1:373205/100‑1 (MQ=255)
   aTTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGAt                                                                                                 <  1:2328512/100‑1 (MQ=255)
    ttGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATg                                                                                                <  2:1957855/100‑1 (MQ=255)
    ttGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATg                                                                                                <  1:2235489/100‑1 (MQ=255)
     tGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGt                                                                                               >  2:1835039/1‑100 (MQ=255)
      gATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTc                                                                                              >  2:4101283/1‑100 (MQ=255)
       aTCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCg                                                                                             <  1:4007783/100‑1 (MQ=255)
        tCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGa                                                                                            >  1:809878/1‑100 (MQ=255)
        tCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGa                                                                                            <  2:896897/100‑1 (MQ=255)
        tCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGa                                                                                            >  2:549238/1‑100 (MQ=255)
         cAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGat                                                                                           <  2:1102839/100‑1 (MQ=255)
          aaaaaCGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGata                                                                                          >  2:3319314/1‑100 (MQ=255)
           aaaaCGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGatat                                                                                         >  1:3202834/1‑100 (MQ=255)
            aaaCGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATc                                                                                        >  1:916199/1‑100 (MQ=255)
             ccgatctttGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACGATATca                                                                                       <  2:3530839/93‑2 (MQ=38)
             aaCGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATcc                                                                                       <  1:2019113/100‑1 (MQ=255)
               cGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATcccc                                                                                     >  1:3520267/1‑100 (MQ=255)
               cGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACGATATcagg                                                                                     <  1:1549350/100‑4 (MQ=38)
               cGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACGATATcagg                                                                                     <  2:1748781/100‑4 (MQ=38)
               cGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACGATATcagg                                                                                     <  2:1411962/100‑4 (MQ=38)
               cGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACGATATcagg                                                                                     <  1:3748507/100‑4 (MQ=38)
                 atatTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCgg                                                                                   <  2:2322211/100‑1 (MQ=255)
                   atTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAg                                                                                 >  1:1845080/1‑100 (MQ=255)
                    ttGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGt                                                                                >  1:1109647/1‑100 (MQ=255)
                    ttGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGt                                                                                >  2:213646/1‑100 (MQ=255)
                    ttGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGt                                                                                <  1:3420892/100‑1 (MQ=255)
                    ttGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGt                                                                                >  1:2707830/1‑100 (MQ=255)
                     tGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGtt                                                                               >  1:825529/1‑100 (MQ=255)
                        aaTCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAAc                                                                            <  1:963067/100‑1 (MQ=255)
                        aaTCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAAc                                                                            >  1:627920/1‑100 (MQ=255)
                         aTCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACg                                                                           <  1:2897137/100‑1 (MQ=255)
                          tCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAActg                                                                          <  1:2845450/100‑3 (MQ=255)
                          tCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACgg                                                                          <  1:3383502/100‑1 (MQ=255)
                          tCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACgg                                                                          <  2:706500/100‑1 (MQ=255)
                           cTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGt                                                                         >  2:72226/1‑100 (MQ=255)
                             tAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTAt                                                                       <  1:2182508/100‑1 (MQ=255)
                              aGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATc                                                                      <  1:72226/100‑1 (MQ=255)
                              aGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCAGAGTTAACGGTATc                                                                      >  2:2855927/1‑100 (MQ=255)
                               gCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATcc                                                                     <  1:482823/100‑1 (MQ=255)
                                      tGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGtt                                                              <  1:2034020/100‑1 (MQ=255)
                                        aCTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAg                                                            <  2:3966306/100‑1 (MQ=255)
                                         cTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGa                                                           >  1:1705430/1‑100 (MQ=255)
                                         cTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGa                                                           <  1:1436319/100‑1 (MQ=255)
                                             aGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACg                                                       >  2:3117718/1‑100 (MQ=255)
                                             aGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACg                                                       >  2:372252/1‑100 (MQ=255)
                                              ggCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGc                                                      <  2:2384716/100‑1 (MQ=255)
                                               gCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCt                                                     >  1:2804789/1‑100 (MQ=255)
                                                cGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTg                                                    <  1:1496165/100‑1 (MQ=255)
                                                 ggAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGa                                                   <  1:213646/100‑1 (MQ=255)
                                                  gAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAg                                                  <  1:3261952/100‑1 (MQ=255)
                                                  gAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAg                                                  <  2:409057/100‑1 (MQ=255)
                                                  gAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAg                                                  <  1:381372/100‑1 (MQ=255)
                                                   aaGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAgg                                                 >  1:4098173/1‑100 (MQ=255)
                                                      tGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAg                                              >  1:1856696/1‑100 (MQ=255)
                                                       gCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAgc                                             <  1:686993/100‑1 (MQ=255)
                                                         cGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAgcgc                                           >  2:1462678/1‑100 (MQ=255)
                                                          gTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGcc                                          <  2:1125221/100‑1 (MQ=255)
                                                           ttCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCa                                         <  1:1669102/100‑1 (MQ=255)
                                                              aGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAata                                      <  1:559896/100‑1 (MQ=255)
                                                               gCGGGTGGAAACACTTAAGCCTGATATCGTCATCCTTGATGTCGATATCACCGGAGTTAACGGTATCCCGGTGTTAGAAACGCTGAGGAAGCGCCAatat                                     >  2:175717/1‑100 (MQ=255)
                                                               gCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAatat                                     >  1:3347922/1‑100 (MQ=255)
                                                                cGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAAtata                                    <  1:2386122/100‑1 (MQ=255)
                                                                  ggTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGc                                  <  2:2955096/100‑1 (MQ=255)
                                                                   gTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCg                                 <  2:42620/100‑1 (MQ=255)
                                                                   gTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCg                                 >  1:4018904/1‑100 (MQ=255)
                                                                    tGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCgg                                <  2:1968712/100‑1 (MQ=255)
                                                                     ggAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGa                               >  1:2670447/1‑100 (MQ=255)
                                                                      gAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGaa                              >  1:1769647/1‑100 (MQ=255)
                                                                       aaaCACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAat                             >  1:571274/1‑100 (MQ=255)
                                                                       aaaCACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAat                             <  1:3563347/100‑1 (MQ=255)
                                                                        aaCACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAatt                            <  1:2217227/100‑1 (MQ=255)
                                                                            cTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAattatta                        >  2:136195/1‑100 (MQ=255)
                                                                            cTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAattatta                        >  2:3459082/1‑100 (MQ=255)
                                                                             ttAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAattattat                       <  2:2878371/100‑1 (MQ=255)
                                                                              tAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAattattatt                      >  2:3860050/1‑100 (MQ=255)
                                                                                ccggggATATCGACATCAATGATGACGATATCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATc                   >  1:1029811/6‑100 (MQ=37)
                                                                                aGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATtattattat                    >  1:3635619/1‑100 (MQ=255)
                                                                                 gCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATc                   <  1:1994921/100‑1 (MQ=255)
                                                                                  ggggATATCGACATCAATGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCg                  >  1:3439819/4‑100 (MQ=38)
                                                                                  ggggATATCGACATCAATGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCg                  >  1:1150087/4‑100 (MQ=38)
                                                                                    ggATATCGACATCAATGATGACGATATCCCCGGTGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTACTATTATCGtc                >  2:2746931/2‑100 (MQ=38)
                                                                                    ggATATCGACATCAATGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATtattattat                    >  2:238148/2‑96 (MQ=39)
                                                                                      atatCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGtctc              <  2:2034337/100‑1 (MQ=255)
                                                                                       tatCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTcc             <  2:3202820/100‑1 (MQ=255)
                                                                                       tatCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTcc             >  2:471140/1‑100 (MQ=255)
                                                                                       tatCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTcc             >  2:3012379/1‑100 (MQ=255)
                                                                                       tatCGACATCAATGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTcc             >  2:3672247/1‑100 (MQ=255)
                                                                                        atCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCg            <  1:2459013/100‑1 (MQ=255)
                                                                                         tCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCGGGTGTTAGAAACGATGAGGAAGCGCCAATATAGCGGAATGATTATTATCGTCTCCGc           >  1:2439873/1‑100 (MQ=255)
                                                                                         tCGTCAGCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGc           <  2:1198394/100‑1 (MQ=255)
                                                                                          cGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCt          >  2:3134144/1‑100 (MQ=255)
                                                                                          cGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCt          <  2:1974592/100‑1 (MQ=255)
                                                                                          cGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCt          >  2:2915184/1‑100 (MQ=255)
                                                                                            acatcaaTGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTaa        >  2:3150935/2‑100 (MQ=255)
                                                                                             catcatTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTaaa       >  2:3057934/1‑100 (MQ=255)
                                                                                              atcatTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTaaaa      >  2:1963819/1‑100 (MQ=255)
                                                                                                  ttGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGa  <  2:3256487/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
TTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGA  >  NC_000913/2483808‑2484005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: