BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,810,759	G→A	100%	intergenic (+80/‑11)	ygaH → / → mprA	putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,810,759	0	G	A	100.0%	506.3 / ‑6.7	147	intergenic (+80/‑11)	ygaH/mprA	putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (73/72); minor base C (2/0); total (75/72)

Only 100 of 147 total aligned reads displayed.

GTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTT  >  NC_000913/2810662‑2810853

gTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATc                                                                                              <  2:3150155/100‑1 (MQ=255)

 tGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCa                                                                                             <  2:3171975/100‑1 (MQ=255)

 tGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCa                                                                                             >  1:1674288/1‑100 (MQ=255)

 tGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCa                                                                                             <  1:3707276/100‑1 (MQ=255)

     ggCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATAcc                                                                                         >  1:2314921/1‑100 (MQ=255)

     ggCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATAcc                                                                                         >  1:1054618/1‑100 (MQ=255)

     ggCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATAcc                                                                                         >  2:2335084/1‑100 (MQ=255)

     ggCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTCGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATAcc                                                                                         >  1:2627364/1‑100 (MQ=255)

       cGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCa                                                                                       >  2:1783922/1‑100 (MQ=255)

       cGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCa                                                                                       >  1:1729698/1‑100 (MQ=255)

       cGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCa                                                                                       >  2:3122409/1‑100 (MQ=255)

             tataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACACAAATgg                                                                                  >  2:2930595/1‑99 (MQ=255)

              ataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGAt                                                                                <  2:1976709/100‑1 (MQ=255)

              ataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGAt                                                                                <  1:443804/100‑1 (MQ=255)

               taACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATa                                                                               <  2:2470346/100‑1 (MQ=255)

                 acacacaACAATCATTTTTCTAAACAAATACATTTACTTTATTTGTCACTGTCCAT‑CTACCGTCGGCTTAAATCAATGAGCTAATACCCAAATgg                                                                                  >  2:199088/4‑95 (MQ=255)

                 aCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGt                                                                             >  2:3267757/1‑100 (MQ=255)

                  cccACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGtt                                                                            >  1:1994159/1‑100 (MQ=255)

                   ccgatctaaTCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTc                                                                           <  1:3770819/93‑1 (MQ=255)

                    cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCg                                                                          >  2:1653938/1‑100 (MQ=255)

                         gAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTa                                                                      <  1:2881118/99‑1 (MQ=255)

                         gAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTAc                                                                     >  1:2178298/1‑100 (MQ=255)

                          aaTCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTcagatcgg                                                                    >  2:3770795/1‑93 (MQ=255)

                           aTCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGc                                                                   >  1:2253100/1‑100 (MQ=255)

                           aTCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGc                                                                   <  2:678126/100‑1 (MQ=255)

                            tCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGcc                                                                  <  1:2930605/100‑1 (MQ=255)

                            tCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGcc                                                                  <  2:3410373/100‑1 (MQ=255)

                            tCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGcc                                                                  <  1:1440253/100‑1 (MQ=255)

                             cATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGcc                                                                  <  1:1078879/99‑1 (MQ=255)

                             cATTTTTCTAAAACAATACATTCACTTTATTTGTCACTGTCGTTACTA‑TAGCGGCTGAAATTAATGAGATGATATCCAAATGGATAGTTAGTTTACGccc                                                                 >  2:186314/1‑100 (MQ=255)

                              ntttttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCa                                                                >  1:2364453/2‑100 (MQ=255)

                              aTTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCa                                                                >  2:1934838/1‑100 (MQ=255)

                                 tttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTg                                                             <  1:1953262/100‑1 (MQ=255)

                                   tCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGaa                                                           >  1:1294812/1‑100 (MQ=255)

                                    cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAAc                                                          <  2:2672488/100‑1 (MQ=255)

                                    cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAAc                                                          <  1:317739/100‑1 (MQ=255)

                                    cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAAc                                                          <  2:1966445/100‑1 (MQ=255)

                                     tAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACa                                                         >  2:617529/1‑100 (MQ=255)

                                      aaaaCAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaa                                                        >  2:1682762/1‑100 (MQ=255)

                                      aaaaCAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaa                                                        >  1:557812/1‑100 (MQ=255)

                                      aaaaCAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaa                                                        >  2:403380/1‑100 (MQ=255)

                                       aaaCAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaaa                                                       <  1:1462770/100‑1 (MQ=255)

                                          cAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGc                                                    >  1:1456987/1‑100 (MQ=255)

                                            aTACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTa                                                  >  2:474925/1‑100 (MQ=255)

                                              aCATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaaa                                                <  2:4090829/100‑1 (MQ=255)

                                                aTTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAAt                                              >  1:907845/1‑100 (MQ=255)

                                                 tttACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAAtt                                             <  1:3236519/100‑1 (MQ=255)

                                                  ttACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAAttt                                            >  2:1192950/1‑100 (MQ=255)

                                                    aCTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                          <  1:634969/100‑1 (MQ=255)

                                                    aCTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                          >  1:3891019/1‑100 (MQ=255)

                                                     cTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                         <  2:3276763/100‑1 (MQ=255)

                                                     cTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                         >  1:3809664/1‑100 (MQ=255)

                                                      tttATTTTTCACTGTCGTTACTA‑TATCTGCTGAAATTAATTAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                         <  2:493496/99‑1 (MQ=255)

                                                      tttATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgcg                                        >  2:76474/1‑100 (MQ=255)

                                                      tttATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgcg                                        <  2:2576711/100‑1 (MQ=255)

                                                       ttATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCgcgc                                       >  2:1684244/1‑100 (MQ=255)

                                                        tATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGcc                                      >  2:1516291/1‑100 (MQ=255)

                                                          tttGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAg                                    >  1:2033589/1‑100 (MQ=255)

                                                            tGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgcc                                  >  2:1327195/1‑100 (MQ=255)

                                                               cACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgccgcc                               >  2:416605/1‑100 (MQ=255)

                                                               cACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgccgcc                               <  2:1059730/100‑1 (MQ=255)

                                                                aCTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCa                              >  2:2142165/1‑100 (MQ=255)

                                                                 cTGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCAc                             <  1:1101888/100‑1 (MQ=255)

                                                                  tGTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACg                            <  2:557811/100‑1 (MQ=255)

                                                                   gTCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGa                           <  2:2186537/100‑1 (MQ=255)

                                                                    tCGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGaa                          >  2:1005936/1‑100 (MQ=255)

                                                                     cGTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAg                         <  1:3757289/100‑1 (MQ=255)

                                                                      gTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGa                        >  2:238155/1‑100 (MQ=255)

                                                                      gTTACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATCGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGa                        >  2:3734114/1‑100 (MQ=255)

                                                                       ttACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAt                       <  1:1854690/100‑1 (MQ=255)

                                                                       ttACTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAt                       <  1:2710193/100‑1 (MQ=255)

                                                                          cTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAtttt                    >  1:4105483/1‑100 (MQ=255)

                                                                          cTA‑TATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAtttt                    >  2:788712/1‑100 (MQ=255)

                                                                            a‑tatCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                  <  2:1116746/100‑1 (MQ=255)

                                                                            a‑tatCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                  <  1:1934841/100‑1 (MQ=255)

                                                                            a‑tatCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                  <  1:3252955/100‑1 (MQ=255)

                                                                            a‑tatCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                  <  2:1628119/100‑1 (MQ=255)

                                                                            a‑tatCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                  <  1:366975/100‑1 (MQ=255)

                                                                            a‑tatCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                  <  2:1287336/100‑1 (MQ=255)

                                                                            a‑tatCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                  >  1:3829307/1‑100 (MQ=255)

                                                                            a‑tatCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCAAGCCGCCACGAAGATTTTcc                  <  2:4059929/100‑1 (MQ=255)

                                                                              tatCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACCAATGCTAAACTTTCGCGCCAGCCGCCACGAAGATTTTCCt                 >  2:3359158/1‑100 (MQ=255)

                                                                               atCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCtt                <  1:395645/100‑1 (MQ=255)

                                                                                tCGGGTGAAAGTAATGAGATCATACCTTAATGGATAGTTTCTTTACGCCCATTGAACAAATGCTCAAATTTCGCGCCAGCCGCCACTAAGATTTTcc                  <  2:1820847/97‑1 (MQ=255)

                                                                                   gCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATcn            <  2:1773240/100‑2 (MQ=255)

                                                                                    ntGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAg           >  1:3843028/2‑100 (MQ=255)

                                                                                     tGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAgg          <  1:3859697/100‑1 (MQ=255)

                                                                                     tGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAgg          <  1:3300830/100‑1 (MQ=255)

                                                                                     tGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAgg          <  1:2454392/100‑1 (MQ=255)

                                                                                      gAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGga         >  1:1216562/1‑100 (MQ=255)

                                                                                       aaaTTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGgag        <  1:3091064/100‑1 (MQ=255)

                                                                                       aaaTTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGgag        <  1:3090726/100‑1 (MQ=255)

                                                                                        aaTTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGgaga       <  2:173608/100‑1 (MQ=255)

                                                                                         aTTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGAt      <  2:1454501/100‑1 (MQ=255)

                                                                                           tAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGCTTTTCCTTATCAGGAGATcc    <  2:2565869/100‑1 (MQ=255)

                                                                                           tAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATcc    <  1:3525432/100‑1 (MQ=255)

                                                                                           tAATGAGATCATACCCAAATGGATAGTTCGTTTACGACCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATcc    >  1:2959993/1‑100 (MQ=255)

                                                                                            aaTGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCt   >  1:1370036/1‑100 (MQ=255)

                                                                                            aaTGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCt   >  2:801563/1‑100 (MQ=255)

                                                                                             aTGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCtt  >  2:3345274/1‑100 (MQ=255)

GTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTA‑TATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTT  >  NC_000913/2810662‑2810853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`