Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,814,175 T→A 19.0% intergenic (+21/+43) emrB → / ← luxS multidrug efflux system protein/S‑ribosylhomocysteine lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,814,1750TA19.0% 466.8 / 95.6 198intergenic (+21/+43)emrB/luxSmultidrug efflux system protein/S‑ribosylhomocysteine lyase
Reads supporting (aligned to +/- strand):  ref base T (68/92);  new base A (14/24);  total (82/116)
Fisher's exact test for biased strand distribution p-value = 5.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.03e-01

Only 100 of 202 total aligned reads displayed.

ATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTT  >  NC_000913/2814076‑2814274
                                                                                                   |                                                                                                   
  tCCTCGTCCTGCTGGGACTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTg                                                                                                   >  1:3411775/1‑100 (MQ=255)
         ccTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGc                                                                                            >  2:1782977/1‑100 (MQ=255)
          ctgctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCt                                                                                           <  2:2120570/100‑1 (MQ=255)
          ctgctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGCt                                                                                           <  1:444331/100‑1 (MQ=255)
           tgctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCtt                                                                                          >  1:155642/1‑100 (MQ=255)
           tgctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGCtt                                                                                          <  1:3129821/100‑1 (MQ=255)
           tgctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGCtt                                                                                          <  2:1041157/100‑1 (MQ=255)
           tgctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGCtn                                                                                          <  2:1356052/100‑2 (MQ=255)
            gctgctgCTGGCGTGGTTTGCTAAACCGCCATTTGGCGCAGTTGGCGGCCGAGGCGTTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGCtt                                                                                          <  2:1043886/93‑1 (MQ=255)
            gctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGCtta                                                                                         <  1:3096153/100‑2 (MQ=255)
            gctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGCtta                                                                                         <  1:3748475/100‑2 (MQ=255)
            gctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGCtta                                                                                         <  2:1922228/100‑2 (MQ=255)
            gctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGCtta                                                                                         <  1:1899990/100‑2 (MQ=255)
             ctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCtttt                                                                                        >  1:2696673/1‑100 (MQ=255)
             ctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCtttt                                                                                        >  2:3219840/1‑100 (MQ=255)
              tgtggCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGCttagt                                                                                       <  2:279098/97‑4 (MQ=38)
              tgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGCttagt                                                                                       <  1:1112301/100‑4 (MQ=39)
                gggCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTc                                                                                     >  2:858958/1‑100 (MQ=255)
                 ggCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCa                                                                                    >  2:2913397/1‑100 (MQ=255)
                  gcgggTGTGGGTTGCTAAGCCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGCttagttg                                                                                     <  2:1014397/95‑6 (MQ=37)
                  gCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCaa                                                                                   <  1:2012112/100‑1 (MQ=255)
                  gCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCa                                                                                    <  1:597224/99‑1 (MQ=255)
                   cTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCaat                                                                                  <  1:3319305/100‑1 (MQ=255)
                   cTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCaat                                                                                  <  1:3223001/100‑1 (MQ=255)
                   cTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCaat                                                                                  <  2:3115450/100‑1 (MQ=255)
                    tGGGGTGGTTTGCTAAACCGCCATTTGGCGAAGGGGGCGGCGGAGGCGCTACGCACTAAGTACAACTAAGCCAGGTGATTGGCACTGGATTTTTTCactt                                                                                 >  2:959164/1‑100 (MQ=255)
                         tgGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATtg                                                                            <  1:1329091/100‑1 (MQ=255)
                              tGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAg                                                                       >  2:3466872/1‑100 (MQ=255)
                                cTAAACCGCCATTTGGCGCAGGTTGCGGCGGAGGCGATTGGCACTAAGTAGAACTCCGCGCGTTCCTATGAACTGTCTTTTTTAAATAAATtgtccag                                                                       >  2:1511006/1‑94 (MQ=255)
                                cTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGAt                                                                     >  1:579074/1‑100 (MQ=255)
                                    aCCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGtt                                                                 <  2:3102082/100‑1 (MQ=255)
                                     ccGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGttt                                                                >  2:3564209/1‑100 (MQ=255)
                                     ccGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGttt                                                                >  2:1627899/1‑100 (MQ=255)
                                      cGCCATTTGGCGCAGGTGGCGGCTGATGCCGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGTCTTTTTTCAATTAATTGTGAAGATAGttt                                                                <  2:267766/99‑1 (MQ=255)
                                      cGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTa                                                               <  2:726103/100‑1 (MQ=255)
                                         cATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTg                                                            <  1:2630256/100‑1 (MQ=255)
                                          aTTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGa                                                           <  1:2070642/100‑1 (MQ=255)
                                           tttGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGAc                                                          <  2:3167415/100‑1 (MQ=255)
                                           tttGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGAc                                                          <  1:98836/100‑1 (MQ=255)
                                            ttGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACt                                                         <  2:1687043/100‑1 (MQ=255)
                                              ggCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCGTGTTCAATTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAg                                                       <  2:219497/100‑1 (MQ=255)
                                               gcgcAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGa                                                      >  1:1282785/1‑100 (MQ=255)
                                               gcgcAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGa                                                      <  2:2628428/100‑1 (MQ=255)
                                                 gcAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGaa                                                     >  2:2539579/1‑98 (MQ=255)
                                                 gcAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGAtg                                                    <  2:3172829/100‑1 (MQ=255)
                                                    ggTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCCATTAATTGTGAAGATAGTTTACTGACTAGATGTGc                                                 >  2:1115236/1‑100 (MQ=255)
                                                     gTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCa                                                <  2:1724488/100‑1 (MQ=255)
                                                     gTGGCGGCGGAGGCGGTGCACACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCa                                                <  2:2358078/100‑1 (MQ=255)
                                                      tGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAg                                               <  1:1257701/100‑1 (MQ=255)
                                                        gcggcgGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGtt                                             <  2:3897810/100‑1 (MQ=255)
                                                         cggcggAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTc                                            >  2:3686984/1‑100 (MQ=255)
                                                          ggcggAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTcc                                           >  1:535950/1‑100 (MQ=255)
                                                          ggcggAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTcc                                           <  2:259438/100‑1 (MQ=255)
                                                          ggcggAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTcc                                           <  1:3948201/100‑1 (MQ=255)
                                                           gcggAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCt                                          <  2:2430879/100‑1 (MQ=255)
                                                           gcggAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCt                                          <  1:1719028/100‑1 (MQ=255)
                                                           gcggAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTATTACAATAAAATAAAAAGCTAGTTTCCCAACTACCTGTGCCGTTTCt                                          <  2:1394560/100‑1 (MQ=255)
                                                            cggAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTg                                         >  2:1086439/1‑100 (MQ=255)
                                                             ggAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCGTTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGc                                        >  1:1012496/1‑100 (MQ=255)
                                                              gAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCa                                       <  1:896551/100‑1 (MQ=255)
                                                              gAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCa                                       <  1:973730/100‑1 (MQ=255)
                                                              gAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCa                                       <  2:2477084/100‑1 (MQ=255)
                                                              gAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAGTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCa                                       >  1:2963783/1‑100 (MQ=255)
                                                                ggCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAAc                                     <  1:311137/100‑1 (MQ=255)
                                                                  cagTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACtt                                   >  1:2829461/3‑100 (MQ=255)
                                                                   ggTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTtc                                  >  1:2198356/1‑100 (MQ=255)
                                                                   ggTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTtc                                  >  2:3864907/1‑100 (MQ=255)
                                                                     tGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTtctc                                <  1:826155/100‑1 (MQ=255)
                                                                         cACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTc                            <  1:2625451/100‑1 (MQ=255)
                                                                          aCTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCg                           <  1:1784497/100‑1 (MQ=255)
                                                                          aCTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCg                           <  1:3605723/100‑1 (MQ=255)
                                                                          aCTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCg                           >  1:3358335/1‑100 (MQ=255)
                                                                           cTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCgg                          <  1:2413123/100‑1 (MQ=255)
                                                                              aGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAg                       <  2:3437821/100‑1 (MQ=255)
                                                                               gTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGt                      >  1:240071/1‑100 (MQ=255)
                                                                               attgaaaaaaGCCAGTTCAAATGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGt                      >  1:972773/9‑100 (MQ=38)
                                                                                 tgaaaaaaGCCAGTTCAAATGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGc                    >  1:3039702/7‑100 (MQ=38)
                                                                                  gaaaaaaGCCAGTTCAAATGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGCTGTTCAGTTACTGCAACTTCTTTTTCGGCa                        >  2:1025320/6‑95 (MQ=25)
                                                                                  gaaaaaaGCCAGTTCAAATGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTCACAGACTAGATGTGCAGTTCCTGCAACTTCACTATAGGCAGTGc                    >  2:3125232/6‑99 (MQ=25)
                                                                                   aaCTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCa                  >  2:4182076/1‑100 (MQ=255)
                                                                                       ctgCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGttc              <  1:2375779/98‑1 (MQ=255)
                                                                                        aGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGGTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGttct             <  2:2618721/100‑1 (MQ=255)
                                                                                        aGCCAGTTCAAATGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGttct             >  1:2108480/1‑100 (MQ=255)
                                                                                        aGCCAGTTCAAATGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCCCTTTCGGCAGTGCCAGttct             >  1:3697064/1‑100 (MQ=255)
                                                                                         gCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGttctt            >  2:3059467/1‑100 (MQ=255)
                                                                                         gCCAGTTCAAATGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGttctt            >  1:3014909/1‑100 (MQ=255)
                                                                                           cAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCg          <  1:2494007/100‑1 (MQ=255)
                                                                                           cAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCg          <  1:3076510/100‑1 (MQ=255)
                                                                                            aGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGt         <  2:487307/100‑1 (MQ=255)
                                                                                            aGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGt         <  1:856818/100‑1 (MQ=255)
                                                                                             gTTCATTTGAACTGGCTTTTTTTTTTTTTTTTTTAAGATAGTAAACTGACTAGATGTTTAGTGTGTGCGACTTCTCTGTCGGCAGTTCCACCTCTTCGt         >  2:1813667/1‑99 (MQ=255)
                                                                                             gTTCATTTGAACTGGCTTTTTTTTTTTTTTTTTGAAGATAGTTTACTGACTAGATTTTCTGTTTCTGCAAGTTCTCTTTCGGCAGTGCCAGTTCTTCGtt        >  2:3284292/1‑100 (MQ=255)
                                                                                             gTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGtt        >  1:3519707/1‑100 (MQ=255)
                                                                                             gTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGtt        >  1:1405915/1‑100 (MQ=255)
                                                                                               tcgtttGAACTGGCTTTTTTCAATGAATGGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTgn      <  2:1773449/97‑2 (MQ=255)
                                                                                               tCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGc      <  1:3567939/100‑1 (MQ=255)
                                                                                               tCATTTGAACTGGCTTTTTTCAATTAATTCTGAAGATACTTTACTTACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGt         <  2:4117200/97‑1 (MQ=255)
                                                                                                 aTTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCt     <  2:1851753/99‑1 (MQ=255)
                                                                                                 aTTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTg    <  1:1633702/100‑1 (MQ=255)
                                                                                                 aTTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTg    <  2:2172161/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
ATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTT  >  NC_000913/2814076‑2814274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: