BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,181,298	A→T	19.4%	H293L (CAT→CTT)	ygiC →	ATP‑Grasp family ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,181,298	0	A	T	19.4%	434.6 / 85.9	188	H293L (CAT→CTT)	ygiC	ATP‑Grasp family ATPase
Reads supporting (aligned to +/- strand): ref base A (90/61); new base T (19/18); total (109/79)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 194 total aligned reads displayed.

GAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGG  >  NC_000913/3181200‑3181397

  agagCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATcc                                                                                                  <  1:643958/100‑1 (MQ=255)

   gagCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGatcatc                                                                                                   <  2:4196423/98‑1 (MQ=255)

   gagCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCg                                                                                                 >  2:3079627/1‑100 (MQ=255)

       aTTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCaaa                                                                                             <  2:2654344/100‑1 (MQ=255)

        ttATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGATGATCATCTTCCGCaaaa                                                                                            <  1:2677189/100‑2 (MQ=39)

        ttATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAAt                                                                                            <  1:3495374/100‑1 (MQ=255)

         tATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGATGATCATCTTCCGCaaaat                                                                                           <  2:244288/100‑3 (MQ=39)

         tATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATg                                                                                           <  1:2113020/100‑1 (MQ=255)

          aTCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGATGATCATCTTCCGCaaaata                                                                                          <  1:4164305/100‑4 (MQ=38)

            ctcCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGATGATCATCTTCCGCaaaataag                                                                                        <  1:1141828/100‑6 (MQ=38)

             tcCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGATGATCATCTTCCGCaaaataagc                                                                                       <  1:2595743/100‑7 (MQ=38)

             tcCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGaaa                                                                                       <  1:494879/100‑1 (MQ=255)

             tcCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGAAAATGGaaa                                                                                       >  1:1302063/1‑100 (MQ=255)

              ccAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGATGATCATCTTCCGCaaaataagcg                                                                                      <  1:514245/100‑8 (MQ=37)

               caacaaGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGaaaaa                                                                                     >  1:4021266/1‑100 (MQ=255)

               caacaaGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGaaaaa                                                                                     >  1:2508904/1‑100 (MQ=255)

                aacaaGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGATGATCATCTTCCGCaaaataagcggg                                                                                    <  2:3589354/100‑10 (MQ=37)

                 acaaGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAata                                                                                   <  2:4032433/100‑1 (MQ=255)

                 acaaGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTCATTTTGCGGATGATCATCTTCCGCaaaataagcgggc                                                                                   <  2:2241059/100‑11 (MQ=25)

                  caaGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAatat                                                                                  >  2:682547/1‑100 (MQ=255)

                  caaGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAatat                                                                                  >  2:2325466/1‑100 (MQ=255)

                   aaGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATAtg                                                                                 <  2:1951185/100‑1 (MQ=255)

                    aGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATAtgt                                                                                >  2:2161894/1‑100 (MQ=255)

                    aGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATAtgt                                                                                >  1:3258075/1‑100 (MQ=255)

                     ggCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATAtgtg                                                                               <  1:2873873/100‑1 (MQ=255)

                     ggCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATAtgtg                                                                               >  1:183436/1‑100 (MQ=255)

                         cTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGtca                                                                           <  1:2846283/100‑3 (MQ=255)

                            cTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAAc                                                                        <  1:2633900/100‑1 (MQ=255)

                             tACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAAcc                                                                       >  2:3931102/1‑100 (MQ=255)

                              aCCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCg                                                                      <  2:2688152/100‑1 (MQ=255)

                               ccGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGa                                                                     >  2:3168741/1‑100 (MQ=255)

                               ccGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTACCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGa                                                                     >  2:4166284/1‑100 (MQ=255)

                                cGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGAt                                                                    >  1:395695/1‑100 (MQ=255)

                                 gCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGAtc                                                                   <  1:2974350/100‑1 (MQ=255)

                                 gCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGAtc                                                                   <  2:846799/100‑1 (MQ=255)

                                 gCTACTGTGGGAGATGTTCCCGAATCACCCGAACCGGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGAtc                                                                   <  2:2489563/100‑1 (MQ=255)

                                     cTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACGGAtcttc                                                                >  2:2909849/1‑99 (MQ=255)

                                      tgtgGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCACATGGAAAAATATGTGGTTAAACCGATCTtctc                                                              >  2:1842711/1‑100 (MQ=255)

                                       gtgGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTcc                                                             >  1:3594296/1‑100 (MQ=255)

                                       gtgGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTcc                                                             <  2:1207723/100‑1 (MQ=255)

                                         gggAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCg                                                           >  1:2220756/1‑100 (MQ=255)

                                           gagaTGTTCCCGAATCACCCGAACCTGCTCCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGTAAAAATATGTGGTTAAACCGATCTTCTCCCGTg                                                         <  2:2523523/100‑1 (MQ=255)

                                               tgtcgccGAAGCACGCGAACCTGCTGCCTGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAAGATGTGGTTAAACCGATCTTCTCCCGTGAAgg                                                     <  2:3635023/95‑1 (MQ=255)

                                               tGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAgg                                                     >  2:3117692/1‑100 (MQ=255)

                                               tGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAgg                                                     <  1:3617558/100‑1 (MQ=255)

                                               tGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAgg                                                     >  1:542183/1‑100 (MQ=255)

                                               tGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAgg                                                     <  1:3523652/100‑1 (MQ=255)

                                               tGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAgg                                                     <  2:4125934/100‑1 (MQ=255)

                                               tGTTCCCGAATCACCCGAACCTGCAGCCCGCTTATTTTGCGGAAGATGATCACCCGCAAATGGGAAAATACGTGGATAAACCGATCTTCTCCCGTGAAgg                                                     >  2:2787335/1‑100 (MQ=255)

                                                gTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGgc                                                    >  1:2157820/1‑100 (MQ=255)

                                                   cccGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCa                                                 >  1:2522162/1‑100 (MQ=255)

                                                    ccGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCaa                                                >  2:3816130/1‑100 (MQ=255)

                                                         tCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACgtg                                           <  1:2487136/100‑1 (MQ=255)

                                                          cACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACgtgt                                          >  1:1416867/1‑100 (MQ=255)

                                                          cACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACgtgt                                          >  2:2977646/1‑100 (MQ=255)

                                                           aCCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTc                                         <  1:2744666/100‑1 (MQ=255)

                                                            cccGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTCAACCGATCTTCTCCCGTGAACGCGCAAACGTGTCg                                        >  1:1572074/1‑100 (MQ=255)

                                                            cccGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCg                                        >  1:3392314/1‑100 (MQ=255)

                                                             ccGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGa                                       >  1:827532/1‑100 (MQ=255)

                                                              cGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGAt                                      >  2:3266886/1‑100 (MQ=255)

                                                                 aCCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCAt                                   <  2:2173295/100‑1 (MQ=255)

                                                                   ctgctgCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTg                                 >  2:620356/1‑100 (MQ=255)

                                                                   ctgctgCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTg                                 <  2:1698777/100‑1 (MQ=255)

                                                                    tgctgcCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTga                                >  2:1649250/1‑100 (MQ=255)

                                                                    tgctgcCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTga                                <  1:917212/100‑1 (MQ=255)

                                                                    tgctgcCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTga                                >  1:1218215/1‑100 (MQ=255)

                                                                       tgaccGCTTATTTTGCGGATGATGTTCATCCACAAATGGAAAAATATGAGGTTAAACCGATCTTCTCCCGTGATGGCGGAAACGTGTCGATCATTg                                 <  2:3602711/93‑1 (MQ=255)

                                                                        gcCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAAc                            >  1:1288356/1‑100 (MQ=255)

                                                                           cGCTTATTTTGCGGAAGATGATCATCCGCAAATGGACAAATATGTGGCTAAACCGCTCTTCTCCCGTGAAGGCGCAACCCTGTCGATCATTGAGAACg                           >  2:1099159/1‑98 (MQ=255)

                                                                           cGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGc                         >  1:3141350/1‑100 (MQ=255)

                                                                           cGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGc                         >  1:3905368/1‑100 (MQ=255)

                                                                           cGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGc                         >  1:3467010/1‑100 (MQ=255)

                                                                            tttccatttGCGGATGATCATCTTCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCa                        >  2:159339/7‑100 (MQ=37)

                                                                            gCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCa                        >  2:113195/1‑100 (MQ=255)

                                                                              tccatttGCGGATGATCATCTTCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCaaa                      >  1:7144/5‑100 (MQ=38)

                                                                               ccatttGCGGATGATCATCTTCCGCGAATGGAAAAATATGTGGTTAAACCGATCGTCTCCCGTGAAGGCGCAAAGGTGACAATCATTGAGAACGGCaga                      >  2:1017302/4‑97 (MQ=37)

                                                                                catttGCGGATGATCATCTTCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAAc                    >  1:3329323/3‑100 (MQ=38)

                                                                                catttGCGGATGATCATCTTCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAAc                    >  1:1506575/3‑100 (MQ=38)

                                                                                catttGCGGATGATCATCTTCCGCAAATGGAAAAATATGTGGTTAAAACGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAAc                    >  2:3796302/3‑100 (MQ=38)

                                                                                catttGCGGATGATCATCTTCCGCAAATGGAAAAAGATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTAGATCATTGAGAACGGCAAAAc                    >  2:1771656/3‑100 (MQ=38)

                                                                                aTTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAAc                    >  1:1096019/1‑100 (MQ=255)

                                                                                 atttGCGGATGATCATCTTCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAAcc                   >  2:91612/2‑100 (MQ=39)

                                                                                   ttGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCAt                 <  2:1506697/100‑1 (MQ=255)

                                                                                    tGCGGATGATCATCTTCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCAtt                >  2:3449743/1‑100 (MQ=255)

                                                                                    tGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCAtt                <  1:1909930/100‑1 (MQ=255)

                                                                                     gCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTg               <  1:37775/100‑1 (MQ=255)

                                                                                     gCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTg               <  2:669346/100‑1 (MQ=255)

                                                                                       ggAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGaa             >  2:3395234/1‑100 (MQ=255)

                                                                                        gAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAag            >  1:88285/1‑100 (MQ=255)

                                                                                        gAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAag            >  1:372303/1‑100 (MQ=255)

                                                                                        gAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAag            <  1:3074243/100‑1 (MQ=255)

                                                                                        gAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAag            >  1:2933947/1‑100 (MQ=255)

                                                                                         aaGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAagc           <  2:1190429/100‑1 (MQ=255)

                                                                                         aaGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAagc           <  1:113195/100‑1 (MQ=255)

                                                                                             tgatCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCg       <  2:1722949/100‑1 (MQ=255)

                                                                                             tgatCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCg       >  2:1398050/1‑100 (MQ=255)

                                                                                             tgatCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCg       <  1:2544465/100‑1 (MQ=255)

                                                                                             tgatCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCg       >  1:1791148/1‑100 (MQ=255)

                                                                                                 catcCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAg   >  1:2926565/1‑100 (MQ=255)

                                                                                                  atcCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAgg  >  2:3456444/1‑100 (MQ=255)

GAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGG  >  NC_000913/3181200‑3181397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`