Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,288,033 T→G 15.6% intergenic (+23/‑57) yraH → / → yraI putative fimbrial‑like adhesin protein/putative periplasmic pilin chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,288,0330TG15.6% 458.3 / 72.8 180intergenic (+23/‑57)yraH/yraIputative fimbrial‑like adhesin protein/putative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base T (82/69);  new base G (15/14);  total (97/83)
Fisher's exact test for biased strand distribution p-value = 8.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 189 total aligned reads displayed.

TATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTA  >  NC_000913/3287934‑3288132
                                                                                                   |                                                                                                   
tATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCt                                                                                                     >  1:1529097/1‑100 (MQ=255)
tATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCt                                                                                                     >  1:3695441/1‑100 (MQ=255)
   cAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTc                                                                                                  >  1:1011205/1‑100 (MQ=255)
      ccGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGa                                                                                               >  2:3871058/1‑100 (MQ=255)
      ccGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGa                                                                                               <  1:3747491/100‑1 (MQ=255)
       cGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGAt                                                                                              >  1:3292405/1‑100 (MQ=255)
         tAGTTCCCTCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTATCGTAACGCTGGATTACCGTTAATACGTTAGCGCGTTATCTGACAGGTCAGATaa                                                                                            <  2:2650475/100‑1 (MQ=255)
          aGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACAGGTCAGATAAc                                                                                           <  1:2938421/100‑1 (MQ=255)
          aGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACAGGTCAGATAAc                                                                                           <  1:3219725/100‑1 (MQ=255)
           gTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGATTACCGTTAATACGTTAGGGCGTTATCTGACAGGTCAGATAACGc                                                                                         <  2:3909807/100‑1 (MQ=38)
              ccATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACAGGTCAGATAACGccc                                                                                       <  1:2786462/100‑1 (MQ=255)
              ccATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACAGGTCAGATAACGccc                                                                                       <  2:1953337/100‑1 (MQ=255)
               cATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCt                                                                                      >  1:2610375/1‑100 (MQ=255)
               cATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCt                                                                                      >  1:2214947/1‑100 (MQ=255)
               cATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCt                                                                                      >  1:1461308/1‑100 (MQ=255)
               cATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCt                                                                                      >  1:536365/1‑100 (MQ=255)
               cATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCt                                                                                      <  1:807532/100‑1 (MQ=255)
                aTCAGACATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACAGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCtt                                                                                     >  2:3967332/1‑100 (MQ=255)
                aTCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCtt                                                                                     >  2:2709320/1‑100 (MQ=255)
                    gAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTttcc                                                                                 >  2:3320571/1‑100 (MQ=255)
                    gAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTttcc                                                                                 >  1:2403053/1‑100 (MQ=255)
                     aaaTCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTttcct                                                                                <  1:3018033/100‑1 (MQ=255)
                        tCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTttccttcc                                                                             <  1:4027930/100‑1 (MQ=255)
                        tCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCATTATCTGACCTGTCAGATAACGCCCTTttccttcc                                                                             >  2:1538134/1‑100 (MQ=255)
                         cACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTCATACGTTGGGGCGTTATCTGACCTGTCAGATAACGCCCTTTtccttcct                                                                            >  1:1575742/1‑100 (MQ=255)
                         cACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTtccttcct                                                                            <  1:2031182/100‑1 (MQ=255)
                           ccccAGGGGAAGCAGACGCTATCGTTAACGTCACGCTAGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCctct                                                                          <  2:674117/100‑1 (MQ=255)
                           ccccAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCctct                                                                          <  2:1342326/100‑1 (MQ=255)
                           ccccAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCctct                                                                          >  1:1911841/1‑100 (MQ=255)
                           ccccAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCctct                                                                          >  1:1860093/1‑100 (MQ=255)
                           ccccAGGGGAAGCAGACGCTATCGTTAACGTAACGCAGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCctct                                                                          >  1:2962869/1‑100 (MQ=255)
                            cccAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCtt                                                                         <  1:456191/100‑1 (MQ=255)
                              cAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTtc                                                                       <  2:3434184/100‑1 (MQ=255)
                                  ggAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTTACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCg                                                                    <  2:2242676/99‑1 (MQ=255)
                                   gAAGCAGACGATATCGTTAACGTAACGATGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATCACGCCCTTTTCCTTCCTCTTTCTCGtt                                                                  <  2:1659018/100‑1 (MQ=255)
                                      gCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTa                                                               >  1:6767/1‑100 (MQ=255)
                                      gCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTa                                                               <  1:1058245/100‑1 (MQ=255)
                                      gCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTa                                                               >  1:1303615/1‑100 (MQ=255)
                                        aGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATc                                                             <  2:2410305/100‑1 (MQ=255)
                                         gACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCa                                                            >  1:4169211/1‑100 (MQ=255)
                                          aCGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAg                                                           <  2:3023021/100‑1 (MQ=255)
                                            gCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGt                                                         <  2:1527717/100‑1 (MQ=255)
                                            gCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGt                                                         <  2:1303614/100‑1 (MQ=255)
                                             caaTCGTTAACGTGACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGtt                                                        >  1:2847249/3‑100 (MQ=255)
                                              tATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGTGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTg                                                       <  2:1854401/100‑1 (MQ=255)
                                               aTCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGa                                                      <  2:2300649/100‑1 (MQ=255)
                                                 cGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGaaa                                                    >  2:3539512/1‑100 (MQ=255)
                                                  gTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGaaaa                                                   >  2:696910/1‑100 (MQ=255)
                                                  gTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGaaaa                                                   <  1:1610688/100‑1 (MQ=255)
                                                  gTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGaaaa                                                   >  1:1684016/1‑100 (MQ=255)
                                                    atctgtaacgCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAAt                                                 <  2:1004334/96‑1 (MQ=255)
                                                        gtaacgCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATagat                                             >  1:1004334/1‑97 (MQ=255)
                                                        gtaacgCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACt                                             <  1:3828355/100‑1 (MQ=255)
                                                        gtaacgCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACt                                             <  1:1973989/100‑1 (MQ=255)
                                                         taacgCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTa                                            <  1:409773/100‑1 (MQ=255)
                                                          aacgCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTAt                                           <  2:3695420/100‑1 (MQ=255)
                                                           acgCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATg                                          <  1:78929/100‑1 (MQ=255)
                                                            cgCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGt                                         >  1:703546/1‑100 (MQ=255)
                                                             gCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTc                                        >  1:1249655/1‑100 (MQ=255)
                                                               tGGATTACAGTTAATACGTTAGAGCGTTATCTGACCTGTCAAATAACGAACTTTTCCTTCCTCTTTCTCGTTGAAAAAAAAAAAAAAATAACTATGTCa                                       <  2:1409561/99‑1 (MQ=255)
                                                                   ttACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACg                                  <  1:892063/100‑1 (MQ=255)
                                                                     aCCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGaa                                >  2:2371800/1‑100 (MQ=255)
                                                                       cGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACa                              >  1:2674226/1‑100 (MQ=255)
                                                                       cGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACa                              <  2:4183305/100‑1 (MQ=255)
                                                                        gTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACa                              >  2:4017905/1‑99 (MQ=255)
                                                                        gTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAAAGCCCTTTTCCTTCCTCTTTCTCGTTGTAAAAAATTGAAAAATAACTATGTCAAAACGAACa                              <  2:118828/99‑1 (MQ=255)
                                                                               cGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGt                      >  2:2170239/1‑100 (MQ=255)
                                                                               cGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGt                      >  1:2262857/1‑100 (MQ=255)
                                                                               cGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGt                      >  1:3846390/1‑100 (MQ=255)
                                                                                gTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTg                     <  2:3243105/100‑1 (MQ=255)
                                                                                gTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTg                     >  1:2181402/1‑100 (MQ=255)
                                                                                 ttAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGa                    <  2:3817045/100‑1 (MQ=255)
                                                                                  tAGGGCGTTATCTGATCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGat                   <  1:4133952/100‑1 (MQ=255)
                                                                                     ggCGTTATCTGACAGGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATAtt                >  1:3012530/1‑100 (MQ=255)
                                                                                     ggCGTTATCTGACAGGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATAtt                >  1:3632469/1‑100 (MQ=255)
                                                                                     ggCGCTATCTTACGTGTCACATAACGCGCTTCTCCTCCCTCGTTCTCGTTGTAGGGGGTTGAAAAATAACTATGTCAAAACGAACATTCGTGGTGat                   <  2:3496126/97‑1 (MQ=255)
                                                                                      gCGTTATCTGACAGGTCAGATAACGCCCTTTTCCTTTATCTTTCTCGTTGGATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGGGGTGATATTa               >  2:751555/1‑100 (MQ=38)
                                                                                      gCGTTATCTGACAGGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTa               >  1:450584/1‑100 (MQ=255)
                                                                                      gCGTTATCTGACAGGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTa               >  1:1673372/1‑100 (MQ=255)
                                                                                       cGTTATCTGACAGGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTaa              >  1:2981012/1‑100 (MQ=255)
                                                                                        gTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAAc             <  2:1461305/100‑1 (MQ=255)
                                                                                         ttATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAAcc            <  2:3916128/100‑1 (MQ=255)
                                                                                         ttATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAAcc            <  2:1596755/100‑1 (MQ=255)
                                                                                          tATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCt           >  1:607598/1‑100 (MQ=255)
                                                                                           aTCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCtt          >  2:1759500/1‑100 (MQ=255)
                                                                                           aTCTGACCTGTCAGATAACGCACTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCtt          <  2:947605/100‑1 (MQ=255)
                                                                                            tCTGACCTGTCAGATACCGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCttg         <  2:2446807/100‑1 (MQ=255)
                                                                                             cTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCttgt        <  1:954597/100‑1 (MQ=255)
                                                                                              tGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCttgtt       >  1:3510178/1‑100 (MQ=255)
                                                                                              tGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCttg         >  2:3725083/1‑98 (MQ=255)
                                                                                               gACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCttgttg      <  1:1754590/100‑1 (MQ=255)
                                                                                               gACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCttgttg      <  1:877828/100‑1 (MQ=255)
                                                                                               gACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATAAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCttgttg      <  2:3107184/100‑1 (MQ=255)
                                                                                                 ccTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTCTCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTtgttgt     >  1:3985812/1‑99 (MQ=255)
                                                                                                 ccTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAACCGAACATTCGCGGTGATATTAACCTTGTtgtg    >  2:611516/1‑100 (MQ=255)
                                                                                                  cTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTgtgt   >  1:59574/1‑100 (MQ=255)
                                                                                                  cTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTgtgt   >  2:1828034/1‑100 (MQ=255)
                                                                                                   tGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTa  >  1:2558045/1‑100 (MQ=255)
                                                                                                   tGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTa  >  1:3814827/1‑100 (MQ=255)
                                                                                                   tGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTCGTTGTGTa  >  2:1377911/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
TATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTA  >  NC_000913/3287934‑3288132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: