Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,505,785 A→T 16.4% I79N (ATT→AAT)  yhfS ← FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,505,7850AT16.4% 513.9 / 89.5 214I79N (ATT→AAT) yhfSFNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base A (96/82);  new base T (13/22);  total (109/105)
Fisher's exact test for biased strand distribution p-value = 9.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.98e-03

Only 100 of 220 total aligned reads displayed.

CCATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCA  >  NC_000913/3505687‑3505884
                                                                                                  |                                                                                                   
 cATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATc                                                                                                   <  2:2175429/100‑1 (MQ=255)
 cATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATc                                                                                                   >  1:2372838/1‑100 (MQ=255)
      gCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGcc                                                                                              <  1:4017856/100‑1 (MQ=255)
      gCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGcc                                                                                              >  2:1374712/1‑100 (MQ=255)
      gCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGcc                                                                                              >  2:570224/1‑100 (MQ=255)
        tCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAg                                                                                            >  1:2105560/1‑100 (MQ=255)
        tCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGATTGAGACAAGCCGCGCGCCCGGCGCGAATCGCGCCAg                                                                                            >  2:2826923/1‑100 (MQ=255)
         cAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGATTCGCGccggg                                                                                           <  2:1018599/100‑4 (MQ=255)
         cAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGATTCGCGccggg                                                                                           <  1:478570/100‑4 (MQ=255)
         cAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGATTCGCGccggg                                                                                           <  2:2107362/100‑4 (MQ=255)
         cAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGATTCGCGccggg                                                                                           <  1:1964018/100‑4 (MQ=255)
         cAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAACCGCGCCAGt                                                                                           >  2:304012/1‑100 (MQ=255)
          aataatAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGATTCGCGccgggc                                                                                          <  2:3646201/100‑5 (MQ=255)
          aataatAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGATTCGCGccgggc                                                                                          <  2:474398/100‑5 (MQ=255)
          aataatAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGATTCGCGccgggc                                                                                          <  2:2543722/100‑5 (MQ=255)
          aataatAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGACCGGCGCGATTCGCGccgggc                                                                                          <  2:856811/100‑5 (MQ=255)
          aataatAACCCGTGTCGTCGGGGAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGt                                                                                           >  2:2386154/1‑99 (MQ=255)
            taataaCCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTccc                                                                                        <  1:2471527/100‑1 (MQ=255)
             aataaCCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGATTCGCGccgggctgg                                                                                       <  1:2882489/100‑8 (MQ=255)
              ataaCCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCcg                                                                                      >  1:526229/1‑100 (MQ=255)
                  cccGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGcc                                                                                  <  2:750851/100‑1 (MQ=255)
                      tgtCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGc                                                                              <  2:4129412/100‑1 (MQ=255)
                       gtcgtcGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCa                                                                             <  1:404979/100‑1 (MQ=255)
                       gcagtcgGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGCGTGAGCAAAGCCGCCCGCCCGGCGCGAATCGCGCCAGTCCCCGCGccc                                                                                 >  2:3427580/4‑96 (MQ=255)
                          gtcgGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCa                                                                          >  2:2629594/1‑100 (MQ=255)
                          gtcgGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCa                                                                          >  1:1515117/1‑100 (MQ=255)
                             gggTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAgcg                                                                       <  2:2460668/100‑1 (MQ=255)
                             gggTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAgc                                                                        >  2:430165/1‑99 (MQ=255)
                                tAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGcgccg                                                                    <  1:3991940/100‑1 (MQ=255)
                                  aaCAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCa                                                                  >  1:755608/1‑100 (MQ=255)
                                   aCAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAg                                                                 <  1:2337217/100‑1 (MQ=255)
                                   aCAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAg                                                                 <  2:1091721/100‑1 (MQ=255)
                                    cAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGc                                                                >  2:3314211/1‑100 (MQ=255)
                                    cAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGc                                                                >  2:195986/1‑100 (MQ=255)
                                      ggCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCt                                                              >  2:2928521/1‑100 (MQ=255)
                                      ggCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCt                                                              >  1:1018569/1‑100 (MQ=255)
                                         gcgTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCtgtg                                                           <  2:1572473/100‑1 (MQ=255)
                                         gcgTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCtgtg                                                           <  1:1249617/100‑1 (MQ=255)
                                         gcgTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCtgtg                                                           <  1:3035249/100‑1 (MQ=255)
                                           gTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCg                                                         <  2:3933826/100‑1 (MQ=255)
                                              aTGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGa                                                      <  1:3151/100‑1 (MQ=255)
                                               tGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGaa                                                     >  1:1177319/1‑100 (MQ=255)
                                               tGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGCGaa                                                     >  2:694861/1‑100 (MQ=255)
                                                gCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGaa                                                     <  2:3605257/99‑1 (MQ=255)
                                                 cACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGaaa                                                    >  2:1990403/1‑99 (MQ=255)
                                                 cACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAAt                                                   <  2:4172333/100‑1 (MQ=255)
                                                 cACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAAt                                                   <  1:2690024/100‑1 (MQ=255)
                                                 cACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAAt                                                   >  2:875176/1‑100 (MQ=255)
                                                 cACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAAt                                                   <  1:137180/100‑1 (MQ=255)
                                                 cACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAAt                                                   >  2:2364891/1‑100 (MQ=255)
                                                  aCCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATg                                                  >  2:1651281/1‑100 (MQ=255)
                                                   ccAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGc                                                 >  2:4100447/1‑100 (MQ=255)
                                                   ccAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGc                                                 >  2:1804433/1‑100 (MQ=255)
                                                   ccAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGc                                                 >  1:1040881/1‑100 (MQ=255)
                                                    cAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCa                                                <  1:875176/100‑1 (MQ=255)
                                                    cAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCa                                                >  1:431338/1‑100 (MQ=255)
                                                    cAGAAGACGCGGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCGAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCa                                                <  2:3512719/100‑1 (MQ=255)
                                                     agaagaCGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATGGCGACAGTCCACGCGCACTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCa                                                >  2:3180706/1‑99 (MQ=255)
                                                     agaagaCGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCAt                                               >  2:2299201/1‑100 (MQ=255)
                                                      gaagaCGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATc                                              <  1:3267467/100‑1 (MQ=255)
                                                        agaCGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATcag                                            >  1:3918151/1‑100 (MQ=255)
                                                          aCGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATcagcc                                          <  2:3423084/100‑1 (MQ=255)
                                                          aCGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATcagcc                                          <  1:4148167/100‑1 (MQ=255)
                                                            gCTTCCCCGTTTTGAGCACATCCGCCACCCCTGCGCGAATCGCGCCATTCCCCGCGCCCTTCACCAGCGCCGCAGCCTGTGCGTGAAATGCATcagc                                           <  2:3450774/97‑1 (MQ=255)
                                                              tGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCa                                      >  2:269001/1‑100 (MQ=255)
                                                               gCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCAc                                     >  1:3074921/1‑100 (MQ=255)
                                                               gCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCAc                                     >  2:2590869/1‑100 (MQ=255)
                                                                 cccGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCt                                   <  1:3633369/100‑1 (MQ=255)
                                                                   cGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGt                                 >  1:1600276/1‑100 (MQ=255)
                                                                    ggTTTGAGCAAAGACGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGtt                                >  1:2846123/1‑100 (MQ=255)
                                                                      tttGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCTCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTcc                              <  2:4055783/100‑1 (MQ=255)
                                                                      tttGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTcc                              <  2:1465164/100‑1 (MQ=255)
                                                                        tGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCCGCCCGTGCGTGAAATGCATCAGCCAGCACCTGTTcc                              <  1:740547/98‑1 (MQ=255)
                                                                         gAGCAAAGCCGTCAGCCCGGCGCAAATCGCGCCAGGCGCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCcaca                           <  2:1202399/100‑1 (MQ=255)
                                                                         gAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCcaca                           >  1:3541382/1‑100 (MQ=255)
                                                                         gAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCcaca                           <  1:2625331/100‑1 (MQ=255)
                                                                           gCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACg                         >  2:3248585/1‑100 (MQ=255)
                                                                           gCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACg                         >  1:3201853/1‑100 (MQ=255)
                                                                           gCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACg                         >  1:378035/1‑100 (MQ=255)
                                                                           gCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCCTGAAATGCATCAGCCAGCACCTGTTCCACACg                         >  2:2941843/1‑100 (MQ=255)
                                                                             aaaGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCt                       >  2:545317/1‑100 (MQ=255)
                                                                              aaGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTg                      <  1:3473471/100‑1 (MQ=255)
                                                                               aGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTgg                     <  1:1797287/100‑1 (MQ=255)
                                                                                       gactggCGCGATTCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAAcgcg             >  2:629530/5‑100 (MQ=255)
                                                                                       gactggCGCGATTCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAAcgcg             >  2:490393/5‑100 (MQ=255)
                                                                                       gCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAAcgcg             <  1:992153/100‑1 (MQ=255)
                                                                                        cccGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAAcgcgc            <  1:3324696/100‑1 (MQ=255)
                                                                                        actggCGCGATTCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAAcgcgc            >  2:3956299/4‑100 (MQ=255)
                                                                                        actggCGCGATTCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAAcgcgc            >  1:2804880/4‑100 (MQ=255)
                                                                                         ctggCGCGATTCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAAcgcgcg           >  1:3315815/3‑100 (MQ=255)
                                                                                         ctggCGCGATTCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAAcgcgcg           >  1:2985812/3‑100 (MQ=255)
                                                                                          cGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCgg          <  1:336577/100‑1 (MQ=255)
                                                                                           ggCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGt         >  2:1861586/1‑100 (MQ=255)
                                                                                           ggCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGt         >  1:2174206/1‑100 (MQ=255)
                                                                                             cgcgAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTg       <  1:2782322/100‑1 (MQ=255)
                                                                                             cgcgAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTg       >  1:932875/1‑100 (MQ=255)
                                                                                              gcgAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGa      <  1:157173/100‑1 (MQ=255)
                                                                                               cgAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTg       >  2:1387970/1‑98 (MQ=255)
                                                                                               cgAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGAt     >  1:3869889/1‑100 (MQ=255)
                                                                                               cgAATCGCGCCAGTCCCCGCGCCCCGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGa      <  2:4181832/99‑1 (MQ=255)
                                                                                                  |                                                                                                   
CCATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCA  >  NC_000913/3505687‑3505884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: