Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,705,970 G→A 28.8% intergenic (‑180/+128) dppB ← / ← dppA dipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,9700GA28.8% 481.4 / 207.3 264intergenic (‑180/+128)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base G (94/94);  new base A (66/10);  total (160/104)
Fisher's exact test for biased strand distribution p-value = 1.28e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.88e-03

Only 100 of 274 total aligned reads displayed.

CAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGC  >  NC_000913/3705871‑3706068
                                                                                                   |                                                                                                          
  nggAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcg                                                                                                          >  2:3149952/2‑100 (MQ=255)
  gggAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcg                                                                                                          <  1:3698137/100‑1 (MQ=255)
  gggAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcg                                                                                                          >  2:2605210/1‑100 (MQ=255)
  gggAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcg                                                                                                          <  2:2500132/100‑1 (MQ=255)
      aTGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGt                                                                                                      <  1:1804447/100‑1 (MQ=255)
       tGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGtt                                                                                                     >  2:1426230/1‑100 (MQ=255)
       tGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGtt                                                                                                     >  2:1832657/1‑100 (MQ=255)
        gAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACTGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTa                                                                                                    >  2:2329423/1‑100 (MQ=255)
        gAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGCTGCGGCGTTa                                                                                                    >  2:3289313/1‑100 (MQ=255)
        gAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTa                                                                                                    <  1:3558612/100‑1 (MQ=255)
          aGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAAc                                                                                                  <  2:2844427/100‑1 (MQ=255)
          aGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAAc                                                                                                  >  1:1242193/1‑100 (MQ=255)
            tATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGc                                                                                                <  2:2808506/100‑1 (MQ=255)
             tctgctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGcc                                                                                               <  2:220927/98‑1 (MQ=255)
              ttGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt                                                                                              <  1:3823233/100‑1 (MQ=255)
                gctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTa                                                                                            >  1:2078527/1‑100 (MQ=255)
                 ctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTAt                                                                                           >  2:3816725/1‑100 (MQ=255)
                  tgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATc                                                                                          >  1:719202/1‑100 (MQ=255)
                   gcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATcc                                                                                         <  1:2383856/100‑1 (MQ=255)
                     cGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCgg                                                                                       <  2:1055623/100‑1 (MQ=255)
                      ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGc                                                                                      >  1:1719889/1‑100 (MQ=255)
                        aTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGcan                                                                                    <  1:3383131/100‑3 (MQ=37)
                         tGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTa                                                                                   >  1:1436854/1‑100 (MQ=255)
                         tGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGCATa                                                                                   <  2:266267/100‑1 (MQ=37)
                          ggCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGCATaa                                                                                  <  2:1746596/100‑2 (MQ=25)
                           gcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACa                                                                                <  1:3050886/100‑1 (MQ=255)
                            cgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaa                                                                               >  1:2723297/1‑100 (MQ=255)
                              cTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaaa                                                                             >  1:2452794/1‑100 (MQ=255)
                               tGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAAt                                                                            >  1:1706231/1‑100 (MQ=255)
                                  aCGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCg                                                                          <  2:2531056/99‑1 (MQ=255)
                                   cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                        <  1:2513006/100‑1 (MQ=255)
                                   cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                        >  2:203146/1‑100 (MQ=255)
                                   cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                        >  2:3014917/1‑100 (MQ=255)
                                   cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                        >  1:649584/1‑100 (MQ=255)
                                    gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                       >  1:1012826/1‑100 (MQ=255)
                                    gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                       <  2:3953558/100‑1 (MQ=255)
                                     cAGAGGCAAAGGGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATACAGGAAGCGGCGTTAACGCCTTATACGGCCTACAAAATCg                                                                          >  2:3057430/1‑96 (MQ=18)
                                       gagGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCaaa                                                                    <  1:1319929/100‑1 (MQ=255)
                                         ggCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAtt                                                                  >  1:36542/1‑100 (MQ=255)
                                         ggCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAtt                                                                  >  2:439764/1‑100 (MQ=255)
                                           cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                                >  1:4195175/1‑100 (MQ=255)
                                             caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                              >  2:3571757/1‑100 (MQ=255)
                                               gcgcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGACTTATCCGGCCTACAAAATCGTGCAAATTCAATAc                                                            >  2:180282/1‑100 (MQ=255)
                                                cgcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                              <  2:3464693/97‑1 (MQ=255)
                                                  catccGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAtt                                                         <  2:3993677/97‑1 (MQ=255)
                                                      cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAg                                                     >  1:2885079/1‑100 (MQ=255)
                                                            taCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAtt‑‑‑tt                                          <  1:2567488/100‑1 (MQ=255)
                                                              cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑g                                      <  1:923343/100‑1 (MQ=255)
                                                                   tgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGc                                 <  1:860543/100‑1 (MQ=255)
                                                                    gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGcc                                >  1:2627761/1‑100 (MQ=255)
                                                                    gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGcc                                >  1:1093793/1‑100 (MQ=255)
                                                                     ttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCGTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCt                               <  2:3725793/100‑1 (MQ=255)
                                                                     ttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCt                               >  1:3679319/1‑100 (MQ=255)
                                                                       gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGa                             >  1:1554201/1‑100 (MQ=255)
                                                                       gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGa                             >  1:3346544/1‑100 (MQ=255)
                                                                       gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGa                             >  2:3358654/1‑100 (MQ=255)
                                                                        aCAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGAt                            >  2:836076/1‑100 (MQ=255)
                                                                         cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATa                           <  2:2174638/100‑1 (MQ=255)
                                                                         cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATa                           >  2:308829/1‑100 (MQ=255)
                                                                            aGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGc                        <  1:3944774/100‑1 (MQ=255)
                                                                              tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCAGAtcggaag                      >  1:1856687/1‑94 (MQ=255)
                                                                                cgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAg                    <  2:512306/100‑1 (MQ=255)
                                                                                cgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAg                    <  1:1347262/100‑1 (MQ=255)
                                                                                cgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACACTGCAGAATT‑‑‑TTC‑‑GTAGGTCTGATAAGCgggg                    <  1:2825256/100‑3 (MQ=255)
                                                                                 gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAg                    <  1:1042068/99‑1 (MQ=255)
                                                                                  cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGACTT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgc                   >  2:1645393/1‑99 (MQ=255)
                                                                                    ttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCa                <  2:36542/100‑1 (MQ=255)
                                                                                     tGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCAt               >  2:1606354/1‑100 (MQ=255)
                                                                                      gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATc              >  1:2118781/1‑100 (MQ=255)
                                                                                      gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATc              >  1:484109/1‑100 (MQ=255)
                                                                                       tgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg            >  1:1495145/6‑100 (MQ=18)
                                                                                       tgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAACATATTGC‑‑AATT‑‑‑CTCTTGTAGGCCTGATAAGCGTAGCGCATCa             >  1:3240502/6‑100 (MQ=18)
                                                                                        gtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg           >  2:2969127/5‑100 (MQ=18)
                                                                                        gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCATACAAAAATCTTGCCAATTCAATATATTGCAGGACC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg            >  2:1031472/5‑100 (MQ=18)
                                                                                         tATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAg            <  2:1484001/99‑1 (MQ=255)
                                                                                          aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAAC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc          >  1:2978924/3‑100 (MQ=18)
                                                                                          aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAAC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc          >  2:1979436/3‑100 (MQ=18)
                                                                                          aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTT‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc          >  1:2418585/3‑100 (MQ=17)
                                                                                           tGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCa         >  2:1385350/1‑100 (MQ=255)
                                                                                           tGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCa         >  2:2714127/1‑100 (MQ=255)
                                                                                           ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAt        >  1:3603086/2‑100 (MQ=18)
                                                                                           ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAt        >  2:1352664/2‑100 (MQ=18)
                                                                                           ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATGCATTGCACGGGC‑‑‑CAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa         >  2:1137533/2‑100 (MQ=17)
                                                                                           ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTT‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa         >  1:3904255/2‑100 (MQ=17)
                                                                                           ggCCGGATAAGGCGTTTACGCCGCATCCGGCATACAAAATCGTGCAAATTCAACATATTGCAATTCT‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg           >  2:776051/2‑98 (MQ=18)
                                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACGAAATCGTGCTAATTCAATATATTGTGGGCTC‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa         >  1:4050688/2‑100 (MQ=21)
                                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATC‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa         >  1:472568/2‑100 (MQ=21)
                                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGCAATCTC‑‑‑CGT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa         >  2:1888515/2‑100 (MQ=18)
                                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGC‑‑AATC‑‑‑TCCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCa         >  1:3767970/2‑100 (MQ=18)
                                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCATACAAGTTCGTGCAAATTCAATAAATTGCAATAT‑‑‑GAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGcga        >  1:3519790/2‑98 (MQ=21)
                                                                                            gCCGGATAAGGCGTTTACGCCGCATCTGGCCTACAAATTCTTCCAAATTCAATATATTGCAGAAAT‑‑‑CAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaa        >  2:4136408/1‑99 (MQ=18)
                                                                                            gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCT‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGctg        >  2:3868412/1‑98 (MQ=18)
                                                                                            gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGC‑‑AATTCAACTT‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCa         >  1:2267381/1‑100 (MQ=21)
                                                                                            gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCATGAGC‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag        >  2:1309585/1‑99 (MQ=17)
                                                                                            gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATC‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaa        >  1:2177069/1‑99 (MQ=21)
                                                                                            gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATT‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaa        >  1:1199639/1‑99 (MQ=17)
                                                                                            gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAGT‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtt       >  1:3139524/1‑100 (MQ=21)
                                                                                             ccGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtt       >  2:1190945/1‑100 (MQ=255)
                                                                                                gATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAttttt    <  2:3679299/100‑1 (MQ=255)
                                                                                                 aTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg   <  1:116989/100‑1 (MQ=255)
                                                                                                   |                                                                                                          
CAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGC  >  NC_000913/3705871‑3706068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: