Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,790,300 A→T 16.3% intergenic (‑219/+20) waaH ← / ← tdh LPS(HepIII)‑glucuronic acid glycosyltransferase/L‑threonine 3‑dehydrogenase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,790,3000AT16.3% 461.0 / 74.8 185intergenic (‑219/+20)waaH/tdhLPS(HepIII)‑glucuronic acid glycosyltransferase/L‑threonine 3‑dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base A (70/84);  new base T (15/15);  total (85/100)
Fisher's exact test for biased strand distribution p-value = 6.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.52e-01

Only 100 of 190 total aligned reads displayed.

TGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGA  >  NC_000913/3790201‑3790399
                                                                                                   |                                                                                                   
tGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGa                                                                                                     >  1:3581012/1‑100 (MQ=255)
 gagaGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGaa                                                                                                    >  1:2251419/1‑100 (MQ=255)
    agcagcTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGTAttc                                                                                                 <  2:3715954/100‑3 (MQ=255)
    agcagcTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATAc                                                                                                 >  2:2395382/1‑100 (MQ=255)
       agcTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAg                                                                                              >  1:2765413/1‑100 (MQ=255)
       agcTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAg                                                                                              <  1:2910698/100‑1 (MQ=255)
       agcTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAg                                                                                              <  1:4007098/100‑1 (MQ=255)
         cTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGcc                                                                                            <  1:3016243/100‑1 (MQ=255)
          tatataAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGcc                                                                                            <  1:469008/99‑1 (MQ=255)
           atataAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGTATTCCAGCCCt                                                                                          <  1:766043/100‑1 (MQ=255)
           atataAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCt                                                                                          >  1:3694559/1‑100 (MQ=255)
           atataAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCt                                                                                          >  1:2146455/1‑100 (MQ=255)
           atataAGAGATCAGCACAATTACCAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCt                                                                                          <  2:1326739/100‑1 (MQ=255)
           atataAGAGATAAGCCAAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACGGATTATCCTCAGATAAAAGGGCTGGTATTCCAGCCCt                                                                                          <  1:615635/100‑1 (MQ=255)
             ataAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTg                                                                                        >  2:1705931/1‑100 (MQ=255)
              taAGAGCTCCGCGCAATTACGAACTCTTTATGACACTGACAGGATTTTACATTTGTACTAGATTATCCTCAGAAAAAAGGGCTGGAAAAGCAGGCCtt                                                                                         >  2:766577/1‑98 (MQ=255)
              taAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTg                                                                                        <  1:836103/99‑1 (MQ=255)
              taAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGt                                                                                       >  2:2681759/1‑100 (MQ=255)
               aaGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGTATTCCAGCCCtttta                                                                                      <  2:499020/100‑4 (MQ=255)
               aaGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGtt                                                                                      <  1:2056131/100‑1 (MQ=255)
                 gaggTCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGTATTCCAGCCCttttatc                                                                                    <  1:856350/100‑6 (MQ=38)
                 gagaTCAGCACACTTACGAACTCGTTATTACACTGACAGAATTTTACATATTTAACAGACTATCCTCAGATAAAAGGGCTGGTATTCCAGCCCtttt                                                                                       <  2:2244552/97‑3 (MQ=255)
                 gagaTCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGTATTCCAGCCCttttatc                                                                                    <  1:1095812/100‑6 (MQ=39)
                 gagaTCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGTATTCCAGCCCttttatc                                                                                    <  1:2495035/100‑6 (MQ=39)
                  agaTCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCgt                                                                                   <  2:3231067/100‑1 (MQ=255)
                    aTCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCgtgt                                                                                 <  1:2536429/100‑1 (MQ=255)
                    aTCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCgtgt                                                                                 <  2:3601895/100‑1 (MQ=255)
                       aGCACAATTACGACCTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGtt                                                                                <  2:3256538/98‑1 (MQ=255)
                       aGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTaa                                                                              >  1:2991835/1‑100 (MQ=255)
                          acaATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATTCCAGCCCTTGTTCGTGTTAATc                                                                            <  2:2437043/99‑1 (MQ=255)
                            aaTTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGTATTAGAATACCAGACCTTGTTAGTGTTAATccaa                                                                         >  2:17142/1‑98 (MQ=255)
                             aTTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAg                                                                        >  1:1054573/1‑100 (MQ=255)
                             aTTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAg                                                                        >  1:1618425/1‑100 (MQ=255)
                              ttACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGc                                                                       <  1:2382049/100‑1 (MQ=255)
                                    acattctattaCACTGACAAGATTTTATCTATTAAACGGGTCATCCTCAAATAAAAGCGCTGGAATAGCAGCCCTTGTTCGTGTTAATCCGCGCTCAg                                                                   <  2:338085/92‑1 (MQ=255)
                                    aCTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGCATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGaa                                                                 <  1:2965982/100‑1 (MQ=255)
                                      tctTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAg                                                                   <  2:1044169/96‑1 (MQ=255)
                                       ctTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGTGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATaa                                                              >  2:1471663/1‑100 (MQ=255)
                                        tttATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAAc                                                             <  2:2990848/100‑1 (MQ=255)
                                        tttATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAAc                                                             <  1:1288713/100‑1 (MQ=255)
                                        tttATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAAc                                                             <  2:3928406/100‑1 (MQ=255)
                                        tttATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAAc                                                             <  2:1145062/100‑1 (MQ=255)
                                         ttattaCACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACt                                                            >  1:3131051/1‑100 (MQ=255)
                                         ttattaCACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACt                                                            >  2:1760264/1‑100 (MQ=255)
                                           attaCACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACttt                                                          >  2:2873656/1‑100 (MQ=255)
                                           attaCACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACttt                                                          >  1:58164/1‑100 (MQ=255)
                                            ttaCACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTc                                                         >  1:4016485/1‑100 (MQ=255)
                                              acacTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTccc                                                       >  2:2353016/1‑100 (MQ=255)
                                               cacTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCg                                                      <  1:1462146/100‑1 (MQ=255)
                                               cacTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCg                                                      >  2:89627/1‑100 (MQ=255)
                                                 cTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGa                                                    >  2:804460/1‑100 (MQ=255)
                                                  tGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCTTGTTAATCCCAGCTCAGAATAACTTTCCCTGa                                                    >  2:3818884/1‑99 (MQ=255)
                                                      aGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGc                                               >  1:2578558/1‑100 (MQ=255)
                                                       ggATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACt                                                  >  2:400316/1‑96 (MQ=255)
                                                       ggATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGcc                                              <  1:3651660/100‑1 (MQ=255)
                                                       ggATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGcc                                              <  1:1526235/100‑1 (MQ=255)
                                                        gATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGccc                                             <  2:2903194/100‑1 (MQ=255)
                                                          ttttACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGa                                           >  1:875320/1‑100 (MQ=255)
                                                              aCATATTTAACAGATTATCCTCAGATAAAAAGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACg                                        <  2:3361283/99‑1 (MQ=255)
                                                               cATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCa                                      <  2:4140197/100‑1 (MQ=255)
                                                                atatTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCAt                                     >  1:2856262/1‑100 (MQ=255)
                                                                  atTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAg                                   <  2:4014747/100‑1 (MQ=255)
                                                                  atTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAg                                   <  1:2938952/100‑1 (MQ=255)
                                                                   tttAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGc                                  <  1:480645/100‑1 (MQ=255)
                                                                    ttAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCg                                 <  1:1705935/100‑1 (MQ=255)
                                                                    ttAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCg                                 <  1:2681766/100‑1 (MQ=255)
                                                                    ttAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCg                                 <  1:1835823/100‑1 (MQ=255)
                                                                    ttAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCg                                 <  1:3730323/100‑1 (MQ=255)
                                                                     tAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGt                                <  1:1498430/100‑1 (MQ=255)
                                                                       aCAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCGCGAACGCATAGCggc                               >  2:3263994/1‑97 (MQ=255)
                                                                        cAGATTATCCTCAGATAAAAGGGGTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCa                              <  2:201514/99‑1 (MQ=255)
                                                                          gATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAg                           <  2:2776710/100‑1 (MQ=255)
                                                                          gATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTCTTCGTGTTAATCCCAGCTCTGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAg                           <  2:2284031/100‑1 (MQ=255)
                                                                                 cTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCt                    >  2:3675307/1‑100 (MQ=255)
                                                                                 cTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCt                    >  1:3262774/1‑100 (MQ=255)
                                                                                 aacacgaacaaGGGCTGGTATTCCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCt                    >  2:1609087/10‑100 (MQ=38)
                                                                                  tCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTg                   >  2:2339706/1‑100 (MQ=255)
                                                                                  tCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTg                   <  2:3805119/100‑1 (MQ=255)
                                                                                  acacgaacaaGGGCTGGTATTCCAGCCCTTTTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGAACGAACGCATAGCGTCAAAGACCTTCTg                   >  2:1903525/9‑100 (MQ=25)
                                                                                   cAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTgg                  >  1:1728305/1‑100 (MQ=255)
                                                                                   cAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTgg                  <  2:2060931/100‑1 (MQ=255)
                                                                                     cgaacaaGGGCTGGTATTCCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGaa                >  2:1286376/6‑100 (MQ=39)
                                                                                      aTAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGaaa               <  1:2439209/100‑1 (MQ=255)
                                                                                       tAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAat              <  1:2030917/100‑1 (MQ=255)
                                                                                       tAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAat              <  1:1141184/100‑1 (MQ=255)
                                                                                       aacaaGGGCTGGTATTCCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAat              >  1:421256/4‑100 (MQ=255)
                                                                                        acaaGGGCTGGTATTCCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAatc             >  1:3530141/3‑100 (MQ=255)
                                                                                        aaaaGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAatc             <  1:3789539/100‑1 (MQ=255)
                                                                                        aaaaGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGACCGAACGCATAGCGTCAAAGCCCTCCTGGACatc             >  1:1960922/1‑100 (MQ=255)
                                                                                         caaGGGCTGGTATTCCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAatca            >  1:3503255/2‑100 (MQ=255)
                                                                                          aaGGGCTGGTATTCCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCGTAGCGTCAAAGCCCTTCTGGAAatcat           >  2:1098689/1‑100 (MQ=255)
                                                                                          aaGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAatcat           >  2:731694/1‑100 (MQ=255)
                                                                                            gggCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCg         >  2:1945423/1‑100 (MQ=255)
                                                                                               cTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATa      >  2:1817106/1‑100 (MQ=255)
                                                                                               cTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATa      >  1:2095790/1‑100 (MQ=255)
                                                                                               cTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATa      <  2:3932915/100‑1 (MQ=255)
                                                                                                tGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATag     >  1:3180851/1‑100 (MQ=255)
                                                                                                 ggAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATaga    >  1:1795692/1‑100 (MQ=255)
                                                                                                 ggAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATaga    <  2:295502/100‑1 (MQ=255)
                                                                                                   aaTACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAgaga  <  1:1950292/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
TGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGA  >  NC_000913/3790201‑3790399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: