BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,392,617	C→G	15.5%	noncoding (35/76 nt)	glyY →	tRNA‑Gly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,392,617	0	C	G	15.5%	445.2 / 76.8	181	noncoding (35/76 nt)	glyY	tRNA‑Gly
Reads supporting (aligned to +/- strand): ref base C (90/63); new base G (19/9); total (109/72)
Fisher's exact test for biased strand distribution p-value = 4.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.25e-01

Only 100 of 187 total aligned reads displayed.

TCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTT  >  NC_000913/4392518‑4392716

  gcgAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCa                                                                                                   <  1:3917418/100‑1 (MQ=255)

  gcgAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCa                                                                                                   <  1:2136097/100‑1 (MQ=255)

  gcgAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCa                                                                                                   >  1:3873566/1‑100 (MQ=255)

  gcgAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCa                                                                                                   >  2:2344885/1‑100 (MQ=255)

   caaGTTCAAGTCTCGTTTCCCGCTCCAAAATTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCa                                                                                                   <  1:2503265/97‑1 (MQ=255)

    gAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAg                                                                                                 <  1:3929240/100‑1 (MQ=255)

    gAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAg                                                                                                 >  1:4168673/1‑100 (MQ=255)

     aGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAgg                                                                                                >  2:3567665/1‑100 (MQ=255)

       ttggattctcGTTTCCGCCTCCAAAATTGGA‑AAGTGCTGGAAGGCACAGACCACCCTAGCGGGAATATCTCAGTTGGTAGAGCACCACCTTGCCAAgtt                                                                                               <  1:2834816/93‑3 (MQ=14)

         cGAGTCTCGTTTCCCGCTCCAAAATTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCgg                                                                                            >  1:755388/1‑100 (MQ=255)

         cGAGTCTCGTTTCCCGCTCCAAAATTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCg                                                                                             <  1:2293285/99‑1 (MQ=255)

          gAGTCTCGTTTCCCGCTCCAAAATTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGGCAAGGTCgtg                                                                                           <  1:1506289/100‑3 (MQ=255)

          gAGTCTCGTTTCCCGCTCCAAAATTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGGCAAGGTCgtg                                                                                           <  1:1543800/100‑3 (MQ=255)

           gattcggcgtCCCCCGCCAAAAATTCAGAAGTGCTGTAATGCACAGACCGCCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCgg                                                                                            <  2:1466236/90‑1 (MQ=1)

           aGTCTCTTTTCCCGCTCCAAAATTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGGCAAGGTCgtgc                                                                                          <  1:3329605/100‑4 (MQ=255)

           aGTCTCGTTTCCCGCTCCAAAATTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCgggg                                                                                          <  1:2973344/100‑1 (MQ=255)

            gTCTCGTTTCCCGCTCCAAAATTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCgggg                                                                                          <  1:518661/99‑1 (MQ=255)

            gTCTCGTTTCCCGCTCCAAAATTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGt                                                                                         <  1:607520/100‑1 (MQ=255)

            gTCTCGTTTCCCCCTCCAAAATTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGGCAAGGTCgtgct                                                                                         <  2:639669/100‑5 (MQ=14)

              gtcctttCCTGCTCCAATATTTAA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGGCAAGGTCgtgctc                                                                                        <  2:3708756/95‑6 (MQ=14)

              ctcGTTTCCCGCTCCAAAATTTGA‑AAGTTCTGTAAGGCACAGACCACCCAATCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGGCAAGGTCgtgctct                                                                                       <  1:1404770/100‑7 (MQ=14)

                cGTTTCCCGCTCCAAAATTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcgcg                                                                                     <  1:3587669/100‑1 (MQ=255)

                cGTTTCCCGCTCCAAAATTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcgcg                                                                                     >  1:1062518/1‑100 (MQ=255)

                cGTTTCCCGCTCCAAAATTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcgcg                                                                                     >  2:1206874/1‑100 (MQ=255)

                cGTTTCCCGCTACAAAATTGAA‑AAGTGCTGTAAGGAACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcgc                                                                                      <  1:459311/99‑1 (MQ=11)

                     cccGCTCCAAAATTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTc                                                                                <  1:2245303/100‑1 (MQ=255)

                      ccGCTCCAAAATTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCg                                                                               >  1:1958990/1‑100 (MQ=255)

                       cGCTCCAAAATTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGa                                                                              >  2:1104246/1‑100 (MQ=255)

                        gCTCCAAAATTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAg                                                                             <  1:878361/100‑1 (MQ=255)

                         cTCCAAAATTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAg                                                                             >  2:2121570/1‑99 (MQ=255)

                           ccAAAATTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtct                                                                          >  1:3704305/1‑100 (MQ=255)

                           ccAAAATTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtct                                                                          >  1:372639/1‑100 (MQ=255)

                            cAAAATTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtctc                                                                         >  1:3654399/1‑100 (MQ=255)

                            cAAAATTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtctc                                                                         >  2:1651611/1‑100 (MQ=255)

                             aaaaTTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGGTCGAGTCTCg                                                                        >  2:3725019/1‑100 (MQ=255)

                              aaaTTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGt                                                                       >  2:1565812/1‑100 (MQ=255)

                                 tttGA‑AAGTGCTGCAAGGCACAGACCTCCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTc                                                                    <  1:2060568/100‑1 (MQ=16)

                                    gA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCg                                                                 >  1:1917361/1‑100 (MQ=38)

                                     gagaGTGCTGTAACGAACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTcc                                                                   <  1:3175723/95‑1 (MQ=255)

                                          tGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCa                                                            >  2:761639/1‑100 (MQ=38)

                                           gCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCaa                                                           >  1:1371030/1‑100 (MQ=38)

                                            cTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCaaa                                                          >  1:3201578/1‑100 (MQ=35)

                                             tGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAt                                                         >  2:3252664/1‑100 (MQ=38)

                                              gTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAtt                                                        >  1:107378/1‑100 (MQ=38)

                                              gTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAtt                                                        >  1:2075064/1‑100 (MQ=38)

                                                aaGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAttct                                                      >  1:2113974/1‑100 (MQ=38)

                                                aaGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAttct                                                      <  1:822623/100‑1 (MQ=38)

                                                   gCACAGACCTCCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCTGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAtt                                                        <  2:2249007/95‑1 (MQ=9)

                                                    cacaGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCCCGACCTTGCCAAGGTCGGCGTCGCGAGTTCGAGCCTCGTTTCCCCCCCCAAAttcttccc                                                  >  2:4058316/1‑98 (MQ=11)

                                                    cacaGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGGGAGTTAGAGTGTCGTTTCCGGCTCCAAAttct                                                      >  2:115927/1‑96 (MQ=11)

                                                     acaGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTtctct                                                 <  1:2670181/100‑1 (MQ=40)

                                                     acaGAACACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTtctc                                                  <  1:1217729/99‑1 (MQ=255)

                                                      caGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTtctctc                                                <  1:3130751/100‑1 (MQ=40)

                                                       aGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCGAAGGTCGGGGTCGCGAGTGCGAGACTCGTTTCCGGCTCCAGATTCTtctct                                                 >  2:744474/1‑98 (MQ=14)

                                                         accaccCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAAt                                             >  1:649920/1‑100 (MQ=255)

                                                          ccaccCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATa                                            >  1:96961/1‑100 (MQ=255)

                                                            accCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATaaa                                          >  2:1727814/1‑100 (MQ=255)

                                                             cccAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATaaaa                                         >  2:3624643/1‑100 (MQ=255)

                                                             cccAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTATCGCGATataa                                         >  2:2770601/1‑100 (MQ=11)

                                                               cAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTGATCTCACTgaaa                                         >  2:3422583/1‑98 (MQ=11)

                                                                  gCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTTTTAACTCAATAAAatatac                                    >  2:1073763/1‑98 (MQ=14)

                                                                   cGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCca                                   >  1:1644986/1‑100 (MQ=255)

                                                                     tgAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATCTGCTCGCACTGAAatgcgc                                    >  2:1938340/2‑93 (MQ=11)

                                                                     ggAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCcaca                                 >  1:3155610/1‑100 (MQ=255)

                                                                         tAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCg                              <  2:606284/99‑1 (MQ=255)

                                                                         tAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGa                             <  2:1829484/100‑1 (MQ=255)

                                                                         tAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGa                             >  2:1412541/1‑100 (MQ=255)

                                                                                gTTGGTAGAGCACGACCTTGCCAAGGACGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATg                      >  1:2548667/1‑100 (MQ=255)

                                                                                  tGGTAGAGCACGACCTTGCCAAGGTCGTGGTCGCTAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCg                    <  2:2668216/100‑1 (MQ=255)

                                                                                  tGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCg                    >  2:3224571/1‑100 (MQ=255)

                                                                                  tGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAACACAATATCATATCCACAGCGCCGCGATGCg                    >  2:2466789/1‑100 (MQ=11)

                                                                                   ggTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAACTCTTCTCTCAATAAAATATCCACACCGACGCGATGCGt                   <  2:2584045/100‑1 (MQ=255)

                                                                                        agCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTg              <  1:705299/100‑1 (MQ=255)

                                                                                        agCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTg              <  1:1938343/100‑1 (MQ=255)

                                                                                        agCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTg              <  1:2466794/100‑1 (MQ=255)

                                                                                        agCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTg              >  1:4193917/1‑100 (MQ=255)

                                                                                        agCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTg              >  2:1592653/1‑100 (MQ=255)

                                                                                        accccGACCTTGGCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTg              >  2:2114205/5‑100 (MQ=255)

                                                                                        accccGACCTTGGCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTg              >  1:1120890/5‑100 (MQ=255)

                                                                                         gCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGc             >  2:920759/1‑100 (MQ=255)

                                                                                         gCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGc             >  2:2086208/1‑100 (MQ=255)

                                                                                         gCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATCTTCTATTGCTGAAATATCCACAGCACCGCAATg                      >  2:3025725/1‑91 (MQ=14)

                                                                                         ccccGACCTTGGCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGc             >  1:887114/4‑100 (MQ=255)

                                                                                         ccccGACCTTGGCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGc             >  1:1796362/4‑100 (MQ=255)

                                                                                         ccccGACCTTGGCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGc             >  2:4040168/4‑100 (MQ=255)

                                                                                         ccccGACCTTGGCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGc             >  1:4066652/4‑100 (MQ=255)

                                                                                         ccccGACCTTGGCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGc             >  2:3476841/4‑100 (MQ=255)

                                                                                         ccccGACCTTGGCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGc             >  1:538954/4‑100 (MQ=255)

                                                                                            cGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTgg          >  1:3282151/1‑100 (MQ=255)

                                                                                            cGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTgg          >  1:2391800/1‑100 (MQ=255)

                                                                                            cGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTgg          >  1:2346292/1‑100 (MQ=255)

                                                                                            cGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTgg          <  2:1975550/100‑1 (MQ=255)

                                                                                               ccTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGttt       >  2:1897142/1‑100 (MQ=255)

                                                                                               ccTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGttt       >  1:540227/1‑100 (MQ=255)

                                                                                                cTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGtttt      <  2:2373991/100‑1 (MQ=255)

                                                                                                 ttGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGttttt     >  2:2851962/1‑100 (MQ=255)

                                                                                                 ttGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGttttt     >  2:1492300/1‑100 (MQ=255)

                                                                                                  tGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTttg    <  1:1056082/100‑1 (MQ=255)

                                                                                                    ccAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTttgtt  >  2:981713/1‑100 (MQ=255)

                                                                                                    ccAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTttgtt  >  2:2152696/1‑100 (MQ=255)

TCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGA‑AAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTT  >  NC_000913/4392518‑4392716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`