Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,424,468 A→C 15.2% intergenic (+82/+48) ulaF → / ← yjfY L‑ribulose 5‑phosphate 4‑epimerase/YhcN family protein, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,424,4680AC15.2% 470.6 / 81.2 189intergenic (+82/+48)ulaF/yjfYL‑ribulose 5‑phosphate 4‑epimerase/YhcN family protein, periplasmic
Reads supporting (aligned to +/- strand):  ref base A (88/72);  new base C (14/15);  total (102/87)
Fisher's exact test for biased strand distribution p-value = 5.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.49e-01

Only 100 of 189 total aligned reads displayed.

TTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTT  >  NC_000913/4424371‑4424565
                                                                                                  |                                                                                                 
ttaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAg                                                                                                 >  1:556559/1‑100 (MQ=255)
 taCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAgg                                                                                                <  1:351670/100‑1 (MQ=255)
 cgatctcAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAgg                                                                                                <  2:4058762/94‑1 (MQ=255)
    gggCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAggggn                                                                                             <  2:4122563/100‑2 (MQ=255)
    gggCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAggggg                                                                                             >  2:934403/1‑100 (MQ=255)
    gggCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAggggg                                                                                             >  2:1421867/1‑100 (MQ=255)
     ggCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAggggga                                                                                            >  1:368917/1‑99 (MQ=255)
     ggCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGgc                                                                                            >  1:2464296/1‑100 (MQ=255)
     ggCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGgc                                                                                            >  1:3726394/1‑100 (MQ=255)
     ggCAGAAGTAGAACACGCGCTGCGGAAATTTCCTACATCGAGAGATAACTGGTCTAATTCCGCAGAAAAAAAAAAAAAAAAAGCCCCCTGCGAAggggg                                                                                             <  2:368917/99‑1 (MQ=255)
       cAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGgcaa                                                                                          >  1:929656/1‑100 (MQ=255)
         gAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCTGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGgcaaa                                                                                         <  2:718363/99‑1 (MQ=255)
         gAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGgcaaag                                                                                        <  1:954059/100‑1 (MQ=255)
          aaGTAGAACACGCGCTGCGTAATTTTCCTTCATCGTGAGATAACTGGCCTAATTCCGCAGCCTTTTTTCAAAAAAAAGCCCCCTTCGCAGGGGgct                                                                                           <  2:3774882/96‑2 (MQ=255)
          aaGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTTCGCAGGGGgcttttt                                                                                       <  2:1086648/100‑6 (MQ=255)
          aaGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTTCGCAGGGGgcttttt                                                                                       <  1:3091020/100‑6 (MQ=255)
            gTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTTCGCAGGGGgcttttttt                                                                                     <  1:1915413/100‑8 (MQ=255)
            gTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTTCGCAGGGGgcttttttt                                                                                     <  2:1555014/100‑8 (MQ=255)
            gTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGgcaacgcaa                                                                                     >  1:3173780/1‑100 (MQ=255)
             tAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTTCGCAGGGGgctttttttt                                                                                    <  1:2264139/100‑9 (MQ=38)
             tAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTTCGCAGGGGgctttttttt                                                                                    <  2:3666319/100‑9 (MQ=38)
             tAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTTCGCAGGGGgctttttttt                                                                                    <  1:2789581/100‑9 (MQ=38)
              aGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTTCGCAGGGGgcttttttttg                                                                                   <  1:2187777/100‑10 (MQ=38)
              aGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTTCGCAGGGGgcttttttttg                                                                                   <  1:1413284/100‑10 (MQ=38)
              aGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAAc                                                                                   >  1:23310/1‑100 (MQ=255)
              aGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAAc                                                                                   >  2:2190969/1‑100 (MQ=255)
                aaCACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAACCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTa                                                                                 >  1:1819134/1‑100 (MQ=255)
                 acacGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCGAAAAAAAGCACCCTGAGAAGGGGGCAAAGCAAACTAt                                                                                >  1:833210/1‑100 (MQ=255)
                 acacGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTAt                                                                                >  1:3945265/1‑100 (MQ=255)
                 acacGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGCGGCAAAGCAAACTAt                                                                                >  1:4125047/1‑100 (MQ=255)
                   acGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATg                                                                               <  1:3540698/100‑1 (MQ=255)
                   acGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATgg                                                                              <  2:2612494/100‑1 (MQ=255)
                    cgcgCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGc                                                                             <  1:3962344/100‑1 (MQ=255)
                    cgcgCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGc                                                                             >  1:1263923/1‑100 (MQ=255)
                        cTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGAAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATg                                                                         <  1:3819726/100‑1 (MQ=255)
                         tGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGt                                                                        >  2:1991842/1‑100 (MQ=255)
                          gCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATg                                                                         <  1:1432375/98‑1 (MQ=255)
                          gCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGtt                                                                       >  1:3052989/1‑100 (MQ=255)
                           cGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGttt                                                                      >  1:1047199/1‑100 (MQ=255)
                             gAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCg                                                                    <  1:2934670/100‑1 (MQ=255)
                                aTTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTg                                                                 >  2:1194541/1‑100 (MQ=255)
                                 tttCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTTGTTgg                                                                <  1:427728/100‑1 (MQ=255)
                                 tttCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTgg                                                                >  1:3297998/1‑100 (MQ=255)
                                 tttCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTgg                                                                >  1:3992589/1‑100 (MQ=255)
                                 tttCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCACTGCGAAGGGGGCAAAGCAAACTATGGACATGTTTCGTTgg                                                                >  2:834033/1‑100 (MQ=255)
                                  ttcctttcTCGGGAGTGAACTGGTCGACATCTTCAGCCATTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGt                                                               <  1:327169/100‑1 (MQ=255)
                                  ttccttccTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGt                                                               <  1:3019781/100‑1 (MQ=255)
                                   tccttcctCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGtt                                                              <  2:3889594/100‑1 (MQ=255)
                                   tccttcctCGGGAGATAACTGGTCTAATTACGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGtt                                                              <  2:2325356/100‑1 (MQ=255)
                                    ccttcctCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTta                                                             <  2:2471967/100‑1 (MQ=255)
                                    ccttcctCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTta                                                             >  1:164403/1‑100 (MQ=255)
                                      ttcctCGGGAGATAACTGGTCTAATTCCGCAGCCGCTTTTCAAAAAAAAGCCCCCTGCGAAAGGGGCAAAGCAAACTATGGcgatggttcgatggtt                                                              >  2:2769567/1‑97 (MQ=255)
                                            gggAGATAACTGGTCTAATTCCGCAGCCGTTTTTCTAATAAATTCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGt                                                     >  2:53165/1‑100 (MQ=255)
                                            gggAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGt                                                     >  1:1105760/1‑100 (MQ=255)
                                            gggAGAGAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGt                                                     <  2:4037607/100‑1 (MQ=255)
                                             ggAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTgg                                                      <  2:1686117/98‑1 (MQ=255)
                                             ggAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTg                                                    >  1:2435028/1‑100 (MQ=255)
                                              gagaTAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGc                                                   <  1:72048/100‑1 (MQ=255)
                                               agaTAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCt                                                  >  1:3750533/1‑100 (MQ=255)
                                                gaTAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTa                                                 >  1:1513822/1‑100 (MQ=255)
                                                  tAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGc                                               >  2:380902/1‑100 (MQ=255)
                                                   aaCTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAATCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCg                                              >  1:1662149/1‑100 (MQ=255)
                                                   aaCTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCg                                              >  1:1292087/1‑100 (MQ=255)
                                                   aaCTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGAGCAAAGCAAACTATGGCAATGTTCGGTTGGTTATACCTGGTGCTAGCg                                              >  2:2046295/1‑100 (MQ=255)
                                                         tCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAat                                        <  1:1458365/100‑1 (MQ=255)
                                                          cTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAata                                       <  2:3953796/100‑1 (MQ=255)
                                                           tAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAatat                                      >  1:782592/1‑100 (MQ=255)
                                                             aTTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATcc                                    >  1:2231374/1‑100 (MQ=255)
                                                             aTTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATcc                                    >  1:3801634/1‑100 (MQ=255)
                                                              ttCCGCCGCCGTTTTTCAACAAAAAGCCCCCTGCGAAGGGGGCAAAGCCAACTATGGCAATTGTTCGTTGGTTATACCTGTTGCTATCGATAAATATCcg                                   >  2:1633942/1‑100 (MQ=255)
                                                              ttCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTAGGGCAATGTTTCGTTGGTTATTCCTGGTGCTAGCGATAAATATCcg                                   <  2:1158317/100‑1 (MQ=255)
                                                                ccGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCcgcg                                 >  2:3302831/1‑100 (MQ=255)
                                                                ccGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCcgcg                                 >  2:2533754/1‑100 (MQ=255)
                                                                 cGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCgcgc                                <  1:3191025/100‑1 (MQ=255)
                                                                  gCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCt                               >  2:3083414/1‑100 (MQ=255)
                                                                   cAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCt                               >  1:392045/1‑99 (MQ=255)
                                                                      ccGTTTTTCAAAAAAAAGGCCACTGCGAAGCGGGGAAAGCAAGCTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCg                           <  2:4181744/100‑1 (MQ=255)
                                                                           tttCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCat                      <  1:283958/100‑1 (MQ=255)
                                                                            ttCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCata                     >  2:1824495/1‑100 (MQ=255)
                                                                              cAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATAtt                   >  1:453792/1‑100 (MQ=255)
                                                                                aaaaaaaaGGCCCCTGCGAAGGGGGCAAAACAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCg                           >  2:1607041/1‑91 (MQ=255)
                                                                                aaaaaaaaGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTg                  >  2:3822559/1‑100 (MQ=255)
                                                                                 aaaaaaaGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGc                 >  2:2406369/1‑100 (MQ=255)
                                                                                 aaaaaaaGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGc                 <  1:2920896/100‑1 (MQ=255)
                                                                                 aaaaaaaGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGc                 >  2:1669031/1‑100 (MQ=255)
                                                                                 aaaaaaaGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGc                 >  2:1234311/1‑100 (MQ=255)
                                                                                   gctttgCCCCCTTCGCAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGCGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCg               >  2:2277234/6‑100 (MQ=255)
                                                                                    ctttgCCCCCTTCGCAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGt              >  1:2533777/5‑100 (MQ=255)
                                                                                    ctttgCCCCCTTCGCAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGt              >  1:1569279/5‑100 (MQ=255)
                                                                                    ctttgCCCCCTTCGCAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGt              >  2:727498/5‑100 (MQ=255)
                                                                                     tttgCCCCCTTCGCAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGtt             >  1:644681/4‑100 (MQ=255)
                                                                                     aaaGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGtt             <  1:2298480/100‑1 (MQ=255)
                                                                                     aaaGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGtt             <  1:2699021/100‑1 (MQ=255)
                                                                                     aaaGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGtt             <  1:3006013/100‑1 (MQ=255)
                                                                                      ttgCCCCCTTCGCAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTa            >  1:2601048/3‑100 (MQ=255)
                                                                                      ttgCCCCCTTCGCAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCACTGGCGTGCATATTGCCGTTa            >  2:621045/3‑100 (MQ=255)
                                                                                        gCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACt          <  1:3852752/100‑1 (MQ=255)
                                                                                         cccccTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTc         >  2:3575837/1‑100 (MQ=255)
                                                                                         cccccTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTc         >  1:3657856/1‑100 (MQ=255)
                                                                                            ccTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTcccc      <  1:753034/100‑1 (MQ=255)
                                                                                                  |                                                                                                 
TTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTT  >  NC_000913/4424371‑4424565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: