Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 681,700 T→A 17.7% intergenic (+37/+23) djlC → / ← hscC J domain‑containing HscC co‑chaperone; Hsc56/Hsp70 family chaperone Hsc62; RpoD‑binding transcription inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913681,7000TA17.7% 463.5 / 82.6 192intergenic (+37/+23)djlC/hscCJ domain‑containing HscC co‑chaperone; Hsc56/Hsp70 family chaperone Hsc62; RpoD‑binding transcription inhibitor
Reads supporting (aligned to +/- strand):  ref base T (84/73);  new base A (19/15);  total (103/89)
Fisher's exact test for biased strand distribution p-value = 8.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 202 total aligned reads displayed.

ATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCA  >  NC_000913/681601‑681799
                                                                                                   |                                                                                                   
 tCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTa                                                                                                    >  2:3976133/1‑100 (MQ=255)
   aGTTTAACTGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTAcg                                                                                                  >  2:1659529/1‑100 (MQ=255)
    gTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTAcgc                                                                                                 >  2:3156871/1‑100 (MQ=255)
    gTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTAc                                                                                                   <  2:2061464/98‑1 (MQ=255)
      ttAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTAcgccg                                                                                               <  1:2963837/100‑1 (MQ=255)
        aaCGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCa                                                                                             >  1:2800504/1‑100 (MQ=255)
          cGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATc                                                                                           <  1:4163751/100‑1 (MQ=38)
           ggTGCTGGTGATAGGTTATGCTCTCGGCGAAATCGCGATGTTGTTTATCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATcc                                                                                          <  1:167098/100‑1 (MQ=38)
           ggTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATcc                                                                                          <  1:1837332/100‑1 (MQ=255)
           ggTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTGGTTTAGCTGACGGTGTGTTTTTTGCGCCGATGCCGGATGGAGCGTTTACGCCGCATcc                                                                                          <  2:1449506/100‑1 (MQ=255)
            gTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCg                                                                                         <  1:1508265/100‑1 (MQ=38)
             tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCgg                                                                                        <  2:3203758/100‑1 (MQ=38)
             tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCgg                                                                                        <  1:3188875/100‑1 (MQ=38)
             tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCgg                                                                                        <  1:3419942/100‑1 (MQ=38)
             tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCgg                                                                                        <  1:1924053/100‑1 (MQ=38)
             tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCgg                                                                                        <  1:1114392/100‑1 (MQ=38)
              gCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGc                                                                                       <  1:922661/100‑1 (MQ=255)
               cTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCGGCa                                                                                      <  1:1437325/100‑1 (MQ=38)
                tGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAg                                                                                     >  1:237094/1‑100 (MQ=255)
                 ggTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCGGCatc                                                                                    <  1:2462905/100‑3 (MQ=37)
                     aTAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCgtg                                                                                <  2:92466/100‑1 (MQ=255)
                       aGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGtt                                                                              <  1:233668/100‑1 (MQ=255)
                       aGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGtt                                                                              <  1:699636/100‑1 (MQ=255)
                         gTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTa                                                                            <  1:1656099/100‑1 (MQ=255)
                           tATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATg                                                                          <  1:786595/100‑1 (MQ=255)
                           gatcttctcGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATg                                                                          <  2:4082912/95‑1 (MQ=255)
                             tGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATggg                                                                        <  2:432933/100‑1 (MQ=255)
                             tGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATggg                                                                        <  1:2198145/100‑1 (MQ=255)
                              gCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTTTTTTTTTTTCTGATGCAGAGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGa                                                                       >  2:1776409/1‑100 (MQ=255)
                               ctctCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGAt                                                                      >  1:3361338/1‑100 (MQ=255)
                               ctctCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGAt                                                                      >  1:1714893/1‑100 (MQ=255)
                                 ctcGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGAtct                                                                    >  1:3145472/1‑100 (MQ=255)
                                 ctcGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGAtct                                                                    >  2:3821596/1‑100 (MQ=255)
                                   cGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCa                                                                  <  2:133851/100‑1 (MQ=255)
                                    ggCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGAtctcca                                                                 >  2:1207105/1‑98 (MQ=255)
                                     gCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGTGtctc                                                                   >  2:1804571/1‑97 (MQ=255)
                                     gCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCa                                                                  >  2:2606027/1‑98 (MQ=255)
                                     gCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAAt                                                                >  2:3460269/1‑100 (MQ=255)
                                      cAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATg                                                               <  2:1967533/100‑1 (MQ=255)
                                      cAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATg                                                               >  2:795132/1‑100 (MQ=255)
                                       aaaaTCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATgg                                                              <  2:615363/100‑1 (MQ=255)
                                         aaTCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCt                                                            >  1:2113589/1‑100 (MQ=255)
                                            cgcgATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTaaa                                                         <  1:3494454/100‑1 (MQ=255)
                                            cgcgATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTaaa                                                         <  1:1832175/100‑1 (MQ=255)
                                             gcgATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAaat                                                        >  1:2384424/1‑100 (MQ=255)
                                              cgATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAaata                                                       >  2:1282795/1‑100 (MQ=255)
                                                 tgttgtTTAGCTGACGATGTGTTTTTTTTTTTTTTTCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCTATGGCTAAATAAtc                                                    >  2:3098271/1‑100 (MQ=37)
                                                  gttgttTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcg                                                   <  1:2254867/100‑1 (MQ=255)
                                                   ttgttTATCTGACGATGTGTTGTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcgc                                                  <  2:3807160/100‑1 (MQ=255)
                                                   ttgttTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcgc                                                  >  1:4075915/1‑100 (MQ=255)
                                                    tgttTAGCTGACGACGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcgct                                                 <  2:2858697/100‑1 (MQ=255)
                                                       ttAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcgctcgc                                              <  1:3460285/100‑1 (MQ=255)
                                                         aGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcc                                             >  2:2578780/1‑99 (MQ=255)
                                                          gCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcccg                                           >  1:2047931/1‑100 (MQ=255)
                                                          gCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcccg                                           <  2:526412/100‑1 (MQ=255)
                                                          gCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGAAGCATCCGGCAGCCGTGTTAATGGGATCTCAATGGCTAAATAATCGCTcgcccg                                           >  1:2051426/1‑100 (MQ=255)
                                                          gCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTAACGCCGCATCCGGCAGCCGCGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcccg                                           >  2:2278534/1‑100 (MQ=255)
                                                           cTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcccgc                                          >  1:3060034/1‑100 (MQ=255)
                                                             gACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCccgccg                                        >  2:106619/1‑100 (MQ=255)
                                                               cGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCccgccgcc                                      >  1:869247/1‑100 (MQ=255)
                                                               cGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCccgccgcc                                      <  1:3913722/100‑1 (MQ=255)
                                                                  tgtgTTTTTTTCTTTTATTCCGGATGCAGCGCTTACGCCGCATCCGGCAGTCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTTGCCCGCCGCCGaa                                   >  2:859161/1‑100 (MQ=255)
                                                                    tgtTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCGGTGTTTATGGGATCTCAATGGATAAATACTCGCTCGCCCGCCCCCGAAc                                  >  2:3853400/1‑99 (MQ=39)
                                                                    tgtTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACt                                 >  1:3016141/1‑100 (MQ=255)
                                                                    tgtTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACt                                 >  1:3486859/1‑100 (MQ=255)
                                                                     gtTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACtt                                >  1:3607299/1‑100 (MQ=255)
                                                                     gtTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACtt                                >  2:2790598/1‑100 (MQ=255)
                                                                     gtTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACtt                                <  2:2118648/100‑1 (MQ=255)
                                                                     gtTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACtt                                >  1:3561498/1‑100 (MQ=255)
                                                                       tttttGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCa                              <  1:2585474/100‑1 (MQ=255)
                                                                        ttttGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCAt                             >  1:3056195/1‑100 (MQ=255)
                                                                        ttttGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCAt                             <  2:2018727/100‑1 (MQ=255)
                                                                             cgcCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCAcg                        >  1:1049716/1‑100 (MQ=255)
                                                                             cgcCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCAcg                        >  1:3252994/1‑100 (MQ=255)
                                                                                cGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTGTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGTCGCCGAACTTCATCCAcgcg                      <  2:143005/99‑1 (MQ=255)
                                                                                  ctGCCGGAGGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATATCAATCGCTAACTATCCGCTCGCCCGCCGCCCACGTTCTTCCAACCGGGc                   >  2:1896693/2‑100 (MQ=12)
                                                                                    gCCGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCa                 >  2:3488905/1‑100 (MQ=38)
                                                                                    gCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGcc                  <  2:2472569/99‑1 (MQ=255)
                                                                                      cGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCata               >  2:393287/1‑100 (MQ=38)
                                                                                      cGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCata               >  1:472080/1‑100 (MQ=38)
                                                                                      cGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCGCGCCCGCCGCCGAACTTCATCCACGCGGGCCata               >  1:1011731/1‑100 (MQ=38)
                                                                                       ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCatat              >  1:1368785/1‑100 (MQ=38)
                                                                                       ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCatat              >  1:2016271/1‑100 (MQ=38)
                                                                                       ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCatat              >  1:375796/1‑100 (MQ=38)
                                                                                       ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCatat              >  2:3800848/1‑100 (MQ=38)
                                                                                        gATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCatatn             <  1:3843090/100‑2 (MQ=255)
                                                                                         aTGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCg            <  1:648589/100‑1 (MQ=255)
                                                                                          tGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGt           <  2:540337/100‑1 (MQ=255)
                                                                                          tGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGt           <  1:1282795/100‑1 (MQ=255)
                                                                                           gCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGtt          <  1:381284/100‑1 (MQ=255)
                                                                                            cAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGttt         >  2:3687923/1‑100 (MQ=255)
                                                                                            cAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCCAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGttt         >  2:626014/1‑100 (MQ=255)
                                                                                             aGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg        <  1:4027391/100‑1 (MQ=255)
                                                                                             aGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg        <  2:3016128/100‑1 (MQ=255)
                                                                                             aGCGTTCACGCCGCATACGGCAGCCGTGTTTATGGGATCTCCATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg        <  1:2282119/100‑1 (MQ=255)
                                                                                              gCGTTTACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCCCCGAACTTCATCCACGCGGGCCATATCGTTTGa       <  2:2749238/100‑1 (MQ=255)
                                                                                               cGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGAc      >  2:2461453/1‑100 (MQ=255)
                                                                                                 tttACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTg    <  2:1142730/100‑1 (MQ=255)
                                                                                                 tttACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTg    >  1:2728900/1‑100 (MQ=255)
                                                                                                 tttACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTg    <  1:2039216/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
ATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCA  >  NC_000913/681601‑681799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: