Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 78,831 T→A 17.7% intergenic (+32/+17) setA → / ← leuD broad specificity sugar efflux system/3‑isopropylmalate dehydratase small subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091378,8310TA17.7% 505.1 / 92.6 214intergenic (+32/+17)setA/leuDbroad specificity sugar efflux system/3‑isopropylmalate dehydratase small subunit
Reads supporting (aligned to +/- strand):  ref base T (93/83);  new base A (10/28);  total (103/111)
Fisher's exact test for biased strand distribution p-value = 3.85e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.61e-01

Only 100 of 219 total aligned reads displayed.

TCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGAC  >  NC_000913/78732‑78930
                                                                                                   |                                                                                                   
  tACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTg                                                                                                   <  1:1032263/100‑1 (MQ=255)
  tACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTg                                                                                                   <  2:1907436/100‑1 (MQ=255)
   aCTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGa                                                                                                  >  1:2053277/1‑100 (MQ=255)
    cTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGAc                                                                                                 >  1:4005368/1‑100 (MQ=255)
     tGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGAcc                                                                                                <  2:4063317/100‑1 (MQ=255)
     tGGGTAATGGCGGTGTTTTCTGTTGTCGCAGTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGAc                                                                                                 <  1:3499937/99‑1 (MQ=255)
      gggTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCg                                                                                               >  2:3081021/1‑100 (MQ=255)
      gggTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCg                                                                                               >  2:224020/1‑100 (MQ=255)
        gTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCggg                                                                                             <  1:655624/100‑1 (MQ=255)
        gTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCggg                                                                                             <  1:273189/100‑1 (MQ=255)
        gTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCggg                                                                                             <  2:3001607/100‑1 (MQ=255)
        gTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCggg                                                                                             <  2:3564276/100‑1 (MQ=255)
        gTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCggg                                                                                             <  2:3980856/100‑1 (MQ=255)
         tAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCTGGTCATTTGACCGGGc                                                                                            >  1:771754/1‑100 (MQ=255)
         tAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCGGGc                                                                                            <  2:387269/100‑1 (MQ=255)
         tAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCGGGc                                                                                            <  1:2156485/100‑1 (MQ=255)
         tAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCGGGc                                                                                            <  1:2311130/100‑1 (MQ=255)
         tAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCGGGc                                                                                            <  1:891741/100‑1 (MQ=255)
         tAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCGGGc                                                                                            <  2:2661280/100‑1 (MQ=255)
         tAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCGGGc                                                                                            <  2:1534480/100‑1 (MQ=255)
          aaTGGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCGGGc                                                                                            <  2:1064476/99‑1 (MQ=255)
           aTTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCGGGctt                                                                                          <  2:1783398/100‑3 (MQ=255)
            ttGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAg                                                                                         >  1:2489395/1‑100 (MQ=255)
              gCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAggg                                                                                       >  1:3182808/1‑100 (MQ=255)
               cGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCTCGGTCATTTGACCGGGCAAgggg                                                                                      >  2:383713/1‑100 (MQ=255)
               cGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAgggg                                                                                      >  1:3337348/1‑100 (MQ=255)
               cGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCGGGctttccg                                                                                      <  1:3228733/100‑7 (MQ=39)
                ggTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGa                                                                                     >  1:3868149/1‑100 (MQ=255)
                ggTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGa                                                                                     >  1:2563107/1‑100 (MQ=255)
                ggTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGa                                                                                     <  2:2053274/100‑1 (MQ=255)
                ggTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCGGGctttccgc                                                                                     <  1:579771/100‑8 (MQ=38)
                ggTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCGGGctttccgc                                                                                     <  2:1724623/100‑8 (MQ=38)
                 gTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGAt                                                                                    <  1:2628031/100‑1 (MQ=255)
                  ttATTTCTGTTGGCGCATTATTTTTAACCGCATAGGTTAAAGACCTTTTATGACGTGGACGATAGCAGAAAGCCCGGTCATTTGACCGTTCAACGGGAtt                                                                                   <  2:2773682/100‑1 (MQ=255)
                       tCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTc                                                                              >  1:1520802/1‑100 (MQ=255)
                       tCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTc                                                                              >  1:1699372/1‑100 (MQ=255)
                        cTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCa                                                                             <  2:2067678/100‑1 (MQ=255)
                        cTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCa                                                                             >  2:2115403/1‑100 (MQ=255)
                         tgttgtCGCATTATTTTTAACCGCAAAGGTTAAAGAGGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCAt                                                                            <  2:2165942/100‑1 (MQ=255)
                         tgttgtCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCAt                                                                            >  2:481861/1‑100 (MQ=255)
                         tgttgtCGCATTATTTTTAACCGCAAAGGGTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCa                                                                             <  1:1190501/99‑1 (MQ=255)
                           ttgtCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGTAAAGCCCGGTCATTTGCCCGGGCAAGGGGATTAATTCAt                                                                            <  2:256366/98‑1 (MQ=255)
                            tgtCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATaaa                                                                         <  1:3701034/100‑1 (MQ=255)
                             gtCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAAc                                                                        >  2:3709537/1‑100 (MQ=255)
                             gtCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAAc                                                                        >  1:2262594/1‑100 (MQ=255)
                             gtCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAAc                                                                        >  2:77931/1‑100 (MQ=255)
                             gtCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGACCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAAc                                                                        >  2:143155/1‑100 (MQ=255)
                             gtCGCATTATTTTTAACCGCAAAGATTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCTGGTCATTTGACCGGGCAAGGGGATTAATTCATAAAc                                                                        >  2:2018680/1‑100 (MQ=255)
                              tagCATTTTGTTTAACCACAAAGGTTAAAGATGTTTGAAATCGTGGATGATAGCGGAAAGCCTGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACg                                                                       <  2:3523689/98‑1 (MQ=255)
                              tCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACg                                                                       <  2:3104981/100‑1 (MQ=255)
                              tCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACg                                                                       <  2:134455/100‑1 (MQ=255)
                               cGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGc                                                                      >  2:1639774/1‑100 (MQ=255)
                                 cATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAg                                                                    <  1:3233090/100‑1 (MQ=255)
                                  attattTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAgg                                                                   >  1:1559756/1‑100 (MQ=255)
                                  attattTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAgg                                                                   >  1:3719207/1‑100 (MQ=255)
                                     attTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGgttg                                                                >  1:1479293/1‑100 (MQ=255)
                                        tttAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGttt                                                             >  1:1616464/1‑100 (MQ=255)
                                         ttAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGtttt                                                            <  1:2062874/100‑1 (MQ=255)
                                         ttAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGtttt                                                            <  1:255360/100‑1 (MQ=255)
                                          tAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTg                                                           <  1:3280769/100‑1 (MQ=255)
                                           aaCCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGc                                                          <  2:964438/100‑1 (MQ=255)
                                           aaCCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGc                                                          <  1:1879412/100‑1 (MQ=255)
                                            aCCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCt                                                         <  1:1260388/100‑1 (MQ=255)
                                            aCCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCt                                                         <  1:2415296/100‑1 (MQ=255)
                                             ccgtacaGGTAAAAGATGTGTGAGGACGAGGACGATGGCGGTAAGCCCGGTCATTTGATCGGGCAAGGGGATTAATGCATAAACGCAGGTTGTTTTGc                                                          <  2:2681530/92‑1 (MQ=255)
                                             ccGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCTGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCt                                                         <  2:366644/99‑1 (MQ=255)
                                             ccGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCtt                                                        >  2:542304/1‑100 (MQ=255)
                                              cGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGcata                                                       >  1:985470/1‑97 (MQ=255)
                                              cGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTc                                                       >  1:2800744/1‑100 (MQ=255)
                                                     gTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCg                                                <  1:2857331/100‑1 (MQ=255)
                                                      ttAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTAATAAACGCAGGTTGTTTTGCTTCATAAGCg                                                >  2:904779/1‑99 (MQ=255)
                                                         aaGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTACTTCATAAACGCAGGATGTTTTGCTTCATAAGCGGCaa                                            >  2:2828487/1‑100 (MQ=255)
                                                           gACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGAGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATg                                          <  2:295157/100‑1 (MQ=255)
                                                             cGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGc                                        >  1:2881598/1‑100 (MQ=255)
                                                                    tgaCGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGcgtcgtcg                                 >  1:2328658/1‑100 (MQ=255)
                                                                       cGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGtgc                              >  1:1564343/1‑100 (MQ=255)
                                                                       cGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGtgc                              <  2:1680125/100‑1 (MQ=255)
                                                                       cGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTAATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGtgc                              >  2:2419750/1‑100 (MQ=255)
                                                                        gTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGtgct                             >  2:2750792/1‑100 (MQ=255)
                                                                        gTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGtgct                             <  1:2281908/100‑1 (MQ=255)
                                                                             cGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAg                        >  1:2130426/1‑100 (MQ=255)
                                                                             cGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGAAAATAATACATAAAAGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAg                        >  2:254821/1‑100 (MQ=255)
                                                                                  gCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAg                   <  2:2216739/100‑1 (MQ=255)
                                                                                    ggAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGcc                 >  2:1937595/1‑100 (MQ=255)
                                                                                    ggAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGcc                 <  1:1370333/100‑1 (MQ=255)
                                                                                    ggAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGcc                 <  2:125320/100‑1 (MQ=255)
                                                                                     gAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGccc                <  2:3337333/100‑1 (MQ=255)
                                                                                     ccttgCCCGGTCAAATGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGccc                >  1:1419064/5‑100 (MQ=255)
                                                                                      cttgCCCGGTCAAATGACAGGGCACGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGcaca               >  2:1059748/4‑100 (MQ=255)
                                                                                        tgCCCGGTCAAATGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAAt             >  1:3591461/2‑100 (MQ=255)
                                                                                         gCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATa            <  1:2602893/100‑1 (MQ=255)
                                                                                         gCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCAGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATa            >  2:2314320/1‑100 (MQ=255)
                                                                                          cccGGTCAAATGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATAc           >  2:1850549/1‑100 (MQ=255)
                                                                                           ccGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACt          >  1:939296/1‑100 (MQ=255)
                                                                                            cGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTg         >  1:819702/1‑100 (MQ=255)
                                                                                               tCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTcc      <  1:4186361/100‑1 (MQ=255)
                                                                                                cATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCa     <  1:2997336/100‑1 (MQ=255)
                                                                                                cATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCa     >  2:1297060/1‑100 (MQ=255)
                                                                                                 aTTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAg    <  2:771754/100‑1 (MQ=255)
                                                                                                 aTTTGACCGGGCAAGGGGATTAATCCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCTCAATACTGTCCAg    <  1:2823201/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
TCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGAC  >  NC_000913/78732‑78930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: