Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,344,033 A→G 16.1% L105P (CTG→CCG)  yciT ← global regulator of transcription; DeoR family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,344,0330AG16.1% 369.5 / 57.3 161L105P (CTG→CCG) yciTglobal regulator of transcription; DeoR family
Reads supporting (aligned to +/- strand):  ref base A (76/59);  new base G (18/8);  total (94/67)
Fisher's exact test for biased strand distribution p-value = 2.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

Only 100 of 169 total aligned reads displayed.

ACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCA  >  NC_000913/1343934‑1344132
                                                                                                   |                                                                                                   
acGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCa                                                                                                     >  1:1848711/1‑100 (MQ=255)
   ccACCGAGCAAAATAACTTCACAAGTCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCagag                                                                                                  <  1:344489/100‑1 (MQ=255)
   ccACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCagag                                                                                                  <  2:2189376/100‑1 (MQ=255)
   ccACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCagag                                                                                                  <  1:940152/100‑1 (MQ=255)
   ccACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTATTCTGCTCGCCCagag                                                                                                  >  2:2454655/1‑100 (MQ=255)
   ccACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCATGATGATAGTGCCATTTTTCTTCTGCTCGCCCaga                                                                                                   >  2:3413431/1‑99 (MQ=255)
     aCCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATACGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTc                                                                                                >  2:2293178/1‑100 (MQ=255)
        gAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCgg                                                                                             <  1:2064365/100‑1 (MQ=255)
         aGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCggg                                                                                            <  1:3860082/100‑1 (MQ=255)
           cAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCg                                                                                          >  2:2361324/1‑100 (MQ=255)
           cAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTGCTGCTCGCCCAGAGTCCGGGc                                                                                           <  2:701201/99‑1 (MQ=255)
            aaaaTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGACGTAGCTGCTGACCGTGATGATACTGACATTTTTCTTCTGCTCGACCAGAGTCCg                                                                                              >  2:398802/1‑95 (MQ=255)
             aaaTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAg                                                                                        >  2:790820/1‑100 (MQ=255)
             aaaTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAg                                                                                        >  2:3783805/1‑100 (MQ=255)
               aTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTa                                                                                      <  2:4099901/100‑1 (MQ=255)
                tAACTTCCCAAGGCGCGTCTTTCAGCAAATGCGCGATCTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTACTCGCCCGGACTCTGGGCGAGCAg                                                                                     <  2:1032464/100‑1 (MQ=38)
                tAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAg                                                                                     >  1:858886/1‑100 (MQ=255)
                tAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAg                                                                                     >  1:4050134/1‑100 (MQ=255)
                  aCTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTACTCGCCCGGACTCTGGGCGAGCAgaa                                                                                   <  2:2191813/100‑3 (MQ=38)
                   cTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATTTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTACTCGCCCGGACTCTGGGCGAgcagaag                                                                                  <  1:3582827/100‑7 (MQ=37)
                   cTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAggg                                                                                   >  1:4059870/1‑99 (MQ=255)
                   cTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGc                                                                                  >  2:2989832/1‑100 (MQ=255)
                   cTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGc                                                                                  >  1:676598/1‑100 (MQ=255)
                    ttCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCGGACTCTGGGCGAgcagaaga                                                                                 <  2:1165965/100‑8 (MQ=38)
                     tCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCAt                                                                                >  2:1558628/1‑100 (MQ=255)
                     tCACAAGGCGCGTCTTTCAGCAAATGCGCGATGAAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCAt                                                                                >  2:2730842/1‑100 (MQ=255)
                      caccAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCAt                                                                                <  1:3481139/99‑1 (MQ=255)
                      cacaAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCAtt                                                                               >  1:1196372/1‑100 (MQ=255)
                        caAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgc                                                                             >  1:3396983/1‑100 (MQ=255)
                        caAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgc                                                                             <  2:1992161/100‑1 (MQ=255)
                        caAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgc                                                                             >  1:3058424/1‑100 (MQ=255)
                             cgcgTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgctgctg                                                                        >  1:2344298/1‑100 (MQ=255)
                             cgcgTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgctgctg                                                                        <  1:1358268/100‑1 (MQ=255)
                             cgcgTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgctgctg                                                                        <  1:1499723/100‑1 (MQ=255)
                              gcgTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgctgctgc                                                                       <  1:1980688/100‑1 (MQ=255)
                              gcgTCTTTCAGCAAATGCGCGATGTAACTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgctgctgc                                                                       >  1:2558770/1‑100 (MQ=255)
                                     tCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTc                                                                >  1:1747715/1‑100 (MQ=255)
                                         ccgatctgcgATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATa                                                            <  1:29938/93‑1 (MQ=255)
                                         cAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCAGCTGCCATTTTCGATa                                                            >  2:2451343/1‑100 (MQ=255)
                                           aaTGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATaaa                                                          >  1:1754736/1‑100 (MQ=255)
                                            tctcgcgATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAg                                                         <  2:512683/97‑1 (MQ=255)
                                                gcgATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATaagatcgg                                                     >  2:29938/1‑94 (MQ=255)
                                                   aTGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGttt                                                  >  1:730275/1‑100 (MQ=255)
                                                       aGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGcc                                              <  1:3524792/100‑1 (MQ=255)
                                                        gctgctGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCCAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGccc                                             <  1:2417874/100‑1 (MQ=255)
                                                          ttctgACCGTGATTATAGTGACATTTTTCTTCTTCTCTCCCATAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGTTAAAGATGGTTTCGCCCgg                                           <  2:2775268/98‑1 (MQ=255)
                                                           gctgACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGt                                          <  2:1919652/100‑1 (MQ=255)
                                                            ctgACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGtt                                         >  1:505613/1‑100 (MQ=255)
                                                            ctgACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGtt                                         <  1:2451348/100‑1 (MQ=255)
                                                            ctgACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGtt                                         >  1:3092887/1‑100 (MQ=255)
                                                              gACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGtt                                         >  2:2112552/1‑98 (MQ=255)
                                                              gACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGa                                       >  1:1122593/1‑100 (MQ=255)
                                                               aCCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGTGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTg                                        <  2:2547554/98‑1 (MQ=255)
                                                               aCCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGaa                                      <  2:646753/100‑1 (MQ=255)
                                                                ccGTGATGATAGTGACATTTTTCTTCTGCTCGCCCATAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAAc                                     <  1:2946081/100‑1 (MQ=255)
                                                                  gTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCa                                   >  2:67967/1‑100 (MQ=255)
                                                                   tgatgaTAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAg                                  <  2:1169619/100‑1 (MQ=255)
                                                                    gatgatAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGc                                 <  2:4151791/100‑1 (MQ=255)
                                                                    gatgatAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGc                                 <  2:3349235/100‑1 (MQ=255)
                                                                    gatgatAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCACGGTTGAACCAGc                                 <  2:3907131/100‑1 (MQ=255)
                                                                      tgatAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGa                               >  1:819194/1‑100 (MQ=255)
                                                                      tgatAGTGAAATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGTCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCTCGCGGTGGAACCATCGa                               >  2:1813796/1‑100 (MQ=255)
                                                                        atAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCAATTTTCGATAAAGATGGTTTCGCACGGTTGAACCAGCGAcg                             >  2:4032203/1‑100 (MQ=255)
                                                                           gTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCgg                          >  1:3895343/1‑100 (MQ=255)
                                                                           gTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCgg                          >  1:510838/1‑100 (MQ=255)
                                                                           gTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCgg                          >  1:1803033/1‑100 (MQ=255)
                                                                              aCATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCaa                       <  2:3396967/100‑1 (MQ=255)
                                                                                aTTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAAc                     <  2:1749686/100‑1 (MQ=255)
                                                                                aTTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAAc                     <  1:2913084/100‑1 (MQ=255)
                                                                                aTTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAAc                     <  2:1747711/100‑1 (MQ=255)
                                                                                  ttttCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTc                   >  2:3999421/1‑100 (MQ=255)
                                                                                   tttCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCg                  <  1:396554/100‑1 (MQ=255)
                                                                                     tgccctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAAACAGCGACGCGGCAAACTCGGc                >  2:3177155/8‑100 (MQ=37)
                                                                                      gccctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCg               >  2:3627994/7‑100 (MQ=38)
                                                                                      cttctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCg               <  1:17305/100‑1 (MQ=255)
                                                                                       ttctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACGAGCGACGCGGCAAACTCGGCGa              <  2:2435565/100‑1 (MQ=255)
                                                                                       ccctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGa              >  1:1483152/6‑100 (MQ=38)
                                                                                        tctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAg             <  1:1097357/100‑1 (MQ=255)
                                                                                        tctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAg             >  1:3069718/1‑100 (MQ=255)
                                                                                        cctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAg             >  2:3244007/5‑100 (MQ=255)
                                                                                        cctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAg             >  2:3194512/5‑100 (MQ=255)
                                                                                        cctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAg             >  1:581771/5‑100 (MQ=255)
                                                                                         ctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGt            >  1:3907625/4‑100 (MQ=255)
                                                                                         ctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGt            >  2:2381612/4‑100 (MQ=255)
                                                                                         ctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACCCg                  >  2:3817382/4‑94 (MQ=38)
                                                                                         ctacTCGCCCGGACTCTGGGCGAGTAGGACATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACTCGGCAAGCTCGGGGAg             >  2:3495978/4‑99 (MQ=255)
                                                                                          tacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTGTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAggt           >  1:3342867/3‑98 (MQ=255)
                                                                                          tGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGtt           >  2:1418938/1‑100 (MQ=255)
                                                                                          tGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGtt           >  1:2288980/1‑100 (MQ=255)
                                                                                           gCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTc          >  1:1218594/1‑100 (MQ=255)
                                                                                           gCTCGCCCAGAGTCCGGGCGAGTAGAGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTc          >  1:1672954/1‑100 (MQ=255)
                                                                                           acTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTc          >  1:4121588/2‑100 (MQ=255)
                                                                                            cTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCa         >  1:3275371/1‑100 (MQ=255)
                                                                                            cTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCa         <  2:3021101/100‑1 (MQ=255)
                                                                                              cGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACg       >  1:3561953/1‑100 (MQ=255)
                                                                                              cGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACg       >  2:2649989/1‑100 (MQ=255)
                                                                                               gCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGc      >  1:2693867/1‑100 (MQ=255)
                                                                                                cccAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCt     >  1:4063207/1‑100 (MQ=255)
                                                                                                  cAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTc   >  1:347579/1‑100 (MQ=255)
                                                                                                   agagTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCa  >  1:1101653/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
ACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCA  >  NC_000913/1343934‑1344132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑